1.Multislice spiral CT of congenital bridging bronchus
Sufang HAN ; Xiucheng GAO ; Zhongfu TIAN ; Wenwei TAGN ; Songyu WEI
Chinese Journal of Radiology 2012;46(2):121-123
Objective To assess the diagnostic value of MSCT in congenital bridging bronchus (BB).Methods Three-dimensional (3D) reconstructed CT images including MPR,MinIP,MIP,VR were respectively analyzed in 12 patients with congenital bridging bronchus on postprocessing workstation.Results Six of 12 BB patients were type Ⅰ bridging bronchus which originated from the left main bronchus at the level of fourth and fifth thoracic vertebral body,while the right bronchus was originated from the normal carina at the level of second and third thoracic vertebral body.The average angle of normal carina was about 59.2°,and the average angle of pseudocarina where BB originated from the left main bronchus was about 108.8°.The left main bronchus proximal to the origin of BB showed significant stenosis,with 1-2 mm width and 17 mm length in the involved segment.Six cases were type Ⅱ bridging bronchus,the right bronchus was absent in normal carina,BB originated at the level of fourth and fifth thoracic vertebral body,the average angle of pseudocarina was about 131°,the whole right lung was supplied by BB.The lower portion of trachea had stenosis in all 6 patients with 2-3 mm width and 30 mm length in the involved segment The lower portion of the trachea was found moving to the left in 4 patients and the left pulmonary artery sling was found in 2 patients.Conclusion MSCT can afford a definite diagnosis of BB by showing the morphology of trachea,bronchus,carina and relationship with surrounding organs with 3D reconstructions.
2.The overcrowding of osteal posterior cranial fossa in adults: multi-slice CT measurements and clinical significance
Zhongfu XIE ; Chao TIAN ; Song JIN ; Tong HAN ; Shimin CUI
Chinese Journal of Radiology 2010;44(3):260-264
Objective To assess the clinical value of MSCT in diagnosing the overcrowding of osteal posterior cranial fossa (PCF) in adults.Methods MSCT images of a cohort of 52 adult patients with foramen magnum osteal malformation confirmed by surgery (diseased group), and 100 healthy adults (control group) were retrospectively reviewed.Images post-processing techniques included multi-planer reformation (MPR) and volume rendering (VR).The posterior cranial fossa volume (PCFV), posterior cranial fossa height (PCFH), clivus length (CL), clivus gradient (CG), supraocciput length (SL), and anteroposterior diameter of the foramen magnum (FMD) were measured on sagittal images in 52 patients and 100 normal adults.Independent-sample student's t test was used to compare the differences between patients and normal adults.Results The results of PCFV, PCFH, CL, SL,FMD and CG, male of control group were (168.2 ±12.3) cm~3, (38.2 ±1.2), (47.1 ±2.8), (41.1 ±1.8), (36.6 ±4.9) mm, (51.5±3.6)°, female of control group were (157.5 ±10.2) cm~3, (36.5 ±1.4), (46.2 ±2.2), (39.7 ±1.3), (35.2 ±3.8), (49.6±3.1)° ;diseased group were (128.7 ±11.7) cm~3, (30.6 ±1.9), (36.2 ±1.4), (37.3 ±0.9), (33.9 ±3.5)mm, (44.5 ±2.8)° .There was significant sex difference in PCFV, PCFH, CL, SL and CG in control group (t =4.70, 6.44, 4.84, 4.43 and 2.81 respectively, P<0.01), but FMD was not significant(t=1.97,P>0.05); the results of PCFV, PCFH, CL, CG and SL were significant different between diseased group and male of control group (t=16.62, 24.04, 25.01, 14.17 and 10.99 respectively,P<0.01) ; the results of PCFV, PCFH, CL, CG and SL were significant different between diseased group and female of control group (t=13.23, 17.80, 27.50, 11.67 and 8.73 respectively,P<0.01) ;but there were no significant differences of FMD between diseased group and control group, both male and female (t=2.96,2.07, P> 0.05).Conclusions The overcrowding of PCF can be accurately measured by MCST.As a routine preoperative examination, MSCT is helpful in the therapeutic selection and the anatomic and pathologic study of PCF.
3.STRUCTURE ELUCIDATION OF GLYCAN OF A GLYCOCONJUGATE SPPA-1 ISOLATED FROM SPIRULINA PLATENSIS
Zhongfu WANG ; Zhiying PENG ; Xuemei PENG ; Linjuan HUANG ; Gengyuan TIAN
Acta Pharmaceutica Sinica 2001;36(5):356-359
AIM To elucidate the structure of the glycan of SPPA-1, a glycoconjugate isolated from Spirulina platensis. METHODS Methylation analysis, GC/MS, and 1D, 2DNMR techniques were used to determine the structures of the glycoconjugate (SPPA-1). RESULTS SPPA-1 was only composed of α-D-glucose and shown to be a (1→4) linked α-D-glucan to which a few glucosyl side chains are attached at O-6 of the glucosyl residues of the main chain. CONCLUSION The glycan of SPPA-1 is a new glucan.
4.STUDIES ON THE GLYCOCONJUGATES AND GLYCANS FROM LYCIUM BARBARUM L IN INHIBITING LOW DENSITY LIPOPROTEIN (LDL) PEROXIDATION
Linjuan HUANG ; Gengyuan TIAN ; Zhongfu WANG ; Jibin DONG ; Manping WU
Acta Pharmaceutica Sinica 2001;36(2):108-111
AIM To determine the effects of glycoconjugates and their glycans from Lycium barbarum L. on inhibiting low density lipoprotein (LDL) peroxidation. METHODS Using Cu2+-induced oxidation as a model, the oxidative production of thiobarbituric acid-reactive substances (TBARS) and the LDL electrophoresis migration on agarose gel were measured. RESULTS The effects of glycoconjugates and their glycans from Lycium barbarum L. on inhibiting LDL peroxidation were different, among them, glycoconjugate LbGp5 showed the best effect on inhibiting LDLperoxidation. CONCLUSION The glycoconjugates can inhibit LDL peroxidatin while their glycans showed no effects on inhibiting LDL peroxidation.
5.Predicting hemorrhagic transformation by microvascular permeability using perfusion CT in acute cerebral infarction in elderly patients
Chao TIAN ; Tianhao YANG ; Zhongfu XIE ; Shimin CUI
Chinese Journal of Geriatrics 2014;33(4):342-345
Objective To study the possibility of microvascular permeability (PS) value derived from perfusion CT (PCT) in predicting hemorrhagic transformation (HT) in acute cerebral infarction in elderly patients.Methods 52 consecutive patients with middle cerebral artery acute ischemic stroke who received thrombolytic therapy were divided into HT group and control group,and patients in HT group were further divided into hemorrhagic infarction (HI) group and parenchymal hematoma (PH) group.PCT data and Alberta stroke program early CT scores (ASPECTS) from CT angiography source images (CTA-SI) achieved within 6 hours after symptom onset of each group was retrospectively analyzed and statistically processed.Results In 52 patients,there were 22 cases developed HT,14 cases with HI,8 cases with PH.Cerebral blood flow (CBF) and cerebral blood volume (CBV) values were decreased and permeability surface area product (PS) was increased in ipsilateral in HT group and control group as compared with the contralateral (all P<0.05).PS value in ipsilateral was significantly higher in HT group than in control group (P<0.01),but CBF and CBV values in affected side showed no significant difference between the HT group and control group.The area under the receiver operator characteristic (ROC) curve of PS Az value was 0.968.When PS ≥5.77 ml · min-1 · 100 g-1,the sensitivity and specificity of predicting HT were 95.5% and 86.7% respectively.The ASPECTS was lower in HT group than in the control group (P<0.01),and ASPECTS was higher in HI group than in PH group (P<0.01).The differences in PCT results in affected side between the HI group and PH group was not significant.Conclusions The significantly increased PS value can be a reference in predicting HT risk and guiding thrombolytic therapy.
6.Isolation,purification,physico-chemical properties and biological activities of a polysaccharide SPPC-1 from Spirulina Platensis
Zhongfu WANG ; Zhiying PENG ; Linjuan HUANG ; Gengyuan TIAN ;
Chinese Journal of Marine Drugs 1994;0(03):-
A polysaccharide SPPC 1 was isolated from spirulina platensis by acetone fractional precipation, free protein was removed by trichloroacetic acid(TCA). Purification of the polysaccharide by Sephadex G 75 colume and CM Sephadex C 50 colume.The homogeneity of this polysaccharide was proved by HPLC and CE. molecular weight was 1.62?10 6. GC showed that the glycoconjugate was composed of Rha,Xyl,Man and Glc. Molar ratio is Rha:Xyl:Man:Glc=6.24:0.37:0.13:0.71. IR, 1HNMR spectrum indicated the presence of ? linkage glycoside. ESR experiment showed that SPPC 1 could eliminate O . 2 radicals.
7.Studies on chemical structure of polysaccharide Ⅰ obtained from Paecilomyces tenuipes
Rong LU ; Lisong SUN ; Zhongfu WANG ; Gengyuan TIAN ; Yoshida TAKASHI ;
Chinese Traditional and Herbal Drugs 1994;0(10):-
Object To study the isolation and purification of a polysaccharide, obtained from Paecilomyces tenuipes Samson, its molecular weight, sugar composition, and mode of linkage Methods Crude polysaccharide was extracted by water at ambient temperature and purified on Sephadex G 100 column Its monosaccharide composition was determined by ionic ion exchange column after complete hydrolysis with acid Their mode of linkage was determined by methylation and glycosidic linkage established by IR and NMR spectra Results HPLC spectrum showed that the polysaccharide was of homogeneous composition, which was also proved latter by GC MS and NMR Conclusion Polysaccharide obtained from P tenuipes Samson is ? (1→6) linked and composed of only D glucose The molecular weight was 2 05?10 4
8.Evaluation of osteal posterior cranial fossa in adults by multi-slice CT measurements before and after operation in basilar invagination patients
Zhongfu XIE ; Chao TIAN ; Song JIN ; Yi WANG ; Tong HAN ; Shimin CUI
Chinese Journal of Radiology 2011;45(12):1151-1154
ObjectiveTo evaluate the clinical effect of MSCT measurements in the pre- and postoperational osteal posterior cranial fossa for the adult patients of basilar invagination.Methods We reviewed the images of a cohort of 31 adult patients with basilar invagination,which were treated by surgical operation.According to the presence of atlantoaxial dislocation,the patients were divided into groups A and B.The basion-dens interval (BDI),atlanto-dental interval (ADI),space available of the spinal cord ( SAC),clivus-canal angle( CCA),Highly index( HI),and Chamberlian line(CBL) of the posterior cranial fossa were obtained in all the patients.Independent-sample Student's t test was used to compare the differences between groups A and groups B.Spearman correlations were analyzed between CT measurement data and effects of operations.ResultsIn Group A,the BDI,ADI,SAC,CCA,HI,CBL before and after surgery were 12.6 mm,8.3 mm,4.5 mm,3.3 mm;18.2 mm,20.8 mm,138.3°,150.4°,28.7 mm,43.4 mm,6.3 mm,3.3 mm respectively.There were significant differences ( t = 5.603,2.323,3.124,5.531,4.278 and 2.375,respectively,P <0.05 ).Preoperative JOA score in groups A was 10 points,and was 14 points after surgery.There was significant difference between the JOA scores before and after surgery ( t = 3.526,P < 0.05 ).There were 7 effective cases and 4 stable cases after surgery in group A.Before and after surgery,JOA score and BDI,ADI,SAC,CCA,HI,CBL were significantly correlated( r = -0.667,- 0.673 ; - 0.571,- 0.619 ; 0.642,0.513 ; 0.525,0.558 ; 0.587,0.511 ; - 0.532,- 0.596,respectively,P<0.05).The SAC,CCA,and CBL before and after surgery in group B were 18.3 mm,19.6 mm,146.8°,150.2°,2.7 mm,1.8 mm.The difference was statistically significant after operation ( t = 5.359,4.126,0.769,P <0.05).The BDI,ADI,and HI before and after surgery in group B were 7.2 mm,6.6 mm,2.4 mm,2.1 mm,39.3 mm,41.5 mm.And there were no significant differences (t = 1.482,2.374,0.153,P>0.05).The preoperative JOA score in groups B was 11 points,and the postoperative score was 16 points.JOA scores before and after surgery were significantly different (t =2.874,P <0.05).There were 14 effective cases and 6 stable cases after operation in group B.The JOA score before and after surgery and BDI,ADI,and HI had no correlation (r =0.341,0.387;0.154,0.182; 0.192,0.167,P >0.05),and CBL,SAC and CCA were correlated (r = -0.756,-0.728;0.651,0.672; 0.726,0.695,P <0.05).ConclusionMSCT measurements for basilar invagination before and after surgery are helpful for understanding changes of osteal posterior fossa anatomy and comprehensive evaluation of surgical treatment.
9.Diagnosis of horseshoe lung with multi-slice helical CT
Zhongfu TIAN ; Ming ZHU ; Suzhen DONG ; Xinrong ZHANG ; Xiaojun ZHANG ; Wenwei TANG
Chinese Journal of Radiology 2011;45(2):153-155
Objective To study the multi-slice helical CT (MSCT) features of horseshoe lung and to improve its diagnostic ability. Methods The clinical and imaging data were retrospectively reviewed in 5 patients. All of them underwent plain MSCT scan, and 4 patients also had enhanced MSCT scan and threedimensional reconstruction. Results In horseshoe lung, the basal segments of the right and left lungs were fused together by a isthmus of pulmonary parenchyma, across the midline behind the pericardium and before the esophagus, descending aorta and spine. All patients were companied with bronchopulmonary dysplasia,anomalous origin of the central bronchi, anomalous arterial supply. Anomalous pulmonary venous return to inferior vena cava and congenital cardiovascular malformations were also found in 4 cases. Conclusion MSCT and three-dimensional reconstruction are valuable for identification of horseshoe lung.
10.Pulmonary expression of HIF-1α and its relationship with GRP78 in the pathogenesis of hepatopulmonary syndrome in rats
Xujiong LI ; Huiying ZHANG ; Xiaoxia TIAN ; Yunxia CHENG ; Li MENG ; Lina LAI ; Zhongfu ZHAO ; Dewu HAN ; Ji CHENG
Journal of Xi'an Jiaotong University(Medical Sciences) 2016;37(4):513-517,524
ABSTRACT:Objective To explore the role of HIF‐1αin the pathogenesis of hepatopulmonary syndrome (HPS) and its relationship with GRP78 .Methods The HPS model in rats was induced by multiple pathogenic factor .The samples were assessed by using Western blotting analysis for HIF‐lα, GRP78 and VEGF164 . The expressions of VEGFR‐2 and CD105 were observed by using immunohistochemical staining .Results The protein level of HIF‐1αwas significantly increased in HPS group at week 8 compared with that at week 4 and 6 groups and corresponding normal control groups .With the development of HPS ,protein level of GRP78 was gradually increased at each time point significantly and reached the highest level at week 8 ;protein level of VEGF164 showed a similar change with GRP78 ,but the peak was at week 6 .Immunohistochemical results showed that the protein expressions of VEGFR‐2 and CD105 were gradually increased in lung tissue as HPS progressed .The protein level of GRP78 was positively correlated with HIF‐1α,VEGF164 ,VEGFR‐2 and CD105 ,respectively (P<0 .05) .Conclusion HIF‐1αis most likely together with GRP78 to play a critical role in promoting pulmonary microvascular remodeling in the pathogenesis of HPS in rats .