Objective To explore the optimal conditions of the vacuum-belt drying process of Rhizoma Chuanxiong extract.Methods Influencing factors of the vacuum belt drying process of Rhizoma Chuanxiong extract were studied by using orthogonal test.The water content and ferulic acid content of dried product were used as the quality indicators.Results The optimal parameters of the vacuum-belt drying process were as follows:the heated region temperature being 95 ℃,the feeding speed at 10 Hz/s,and the belt speed at10 Hz/s.Conclusion The vacuum-belt drying techniquefor drying the extract brings high drying rate and high quality product.This study can provide a reasonable basis for industrial production in line with the GMP requirements.

Objective To observe the microfiltration of different types of ceramic membrane for aqueous extract of Rhizoma Chuanxiong,and to select the optimal type of ceramic membranes. Methods The aqueous extract of Rhizoma Chuanxiong was microfiltered by inorganic ceramic membranes in the pore diameter of 50 nm,200 nm,and 500 nm,respectively. The characters,total solids,and active ingredients of the aqueous extract were analyzed and compared before and after microfiltration. Results The aqueous extract turned into clarified liquid from turbid liquid after microfiltration with the three types of the inorganic ceramic membrane. The removal rate for total solids was 25.7 %,26.5 %and 25.9 %,and the loss rate for ferulic acid was 12 %,6 %and 4 %,respectively for the inorganic ceramic membranes in the pore diameter of 50 nm,200 nm,and 500 nm. Conclusion Both the yield of dry solid material and ferulic acid are stable during the three middle-scale tests,indicating that he aperture ceramic membrane in the pore diameter of 200 nm is a optimal selection for the purification and refining of aqueous extract of Rhizoma Chuanxiong.

AIM: To study the distribution of CYP2C9∗3 and VKORC1-1639G>A gene polymorphism in Han population in Anhui province and their influence on the stable dose of warfarin. METHODS: The blood samples of 1 169 patients from 6 tertiary general hospitals in 5 areas of Anhui province from January 2020 to December 2021 were selected, the genotype of CYP2C9∗3 and VKORC1-1639G>A was detected by fluorescent staining in situ hybridization technique. RESULTS: The distribution of CYP2C9∗3 genotypes in 1 169 patients: the frequencies of AA, AC and CC genes were 90.16%, 9.24% and 0.60%, respectively; The distribution of VKORC1 genotype: the frequencies of AA, AG and GG genes were 84.26%, 14.71% and 1.03% respectively; There was no significant difference between the two genotypes in gender, age and regional distribution (P>0.05). The average daily warfarin dose of CYP2C9∗3 AA genotype in 755 patients with stable warfarin dose was (3.02±0.59) mg/d, which was significantly higher than patients with AC genotype and CC genotype; The average daily warfarin dose of patients with VKORC1-1639AA genotype was (2.72±0.40) mg/d, which was significantly lower than that of patients with AG genotype and GG genotype (P<0.05). And the difference was statistically significant (P<0.05); There are significant differences in gender, age and clinical diagnosis between patients with stable dose of warfarin and those without stable dose (P<0.05). CONCLUSION: CYP2C9 and VKORC1 genotypes are associated with the stable dose of warfarin. Clinical anticoagulation therapy guided by CYP2C9 and VKORC1 genotypes can provide guidance for individualized medication of warfarin.