2.Pilomatricoma: a retrospective study of 399 cases.
Yongwei GUO ; Xia ZHAO ; Zhongwen ZHOU ; Zhongchun CHEN ; Songmin LIANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2013;27(21):1211-1213
OBJECTIVE:
To study the clinical and pathological features of pilomatricoma.
METHOD:
The authors retrospectively investigated the clinical and pathological materials of 399 patients with pilomatricoma.
RESULT:
Single lesion occurred in most patients (99%) and 56.39% of them were younger than 30 years. The male-female ratio was 1:1.33. The lesions which sizes average 1.22 cm were commonly emerged in the head, neck, and upper extremity.
CONCLUSION
Pilomatricoma is a slowly developed benign cutaneous tumour, but it can aggravate sometimes. It's manifestation is diversed and easily misdiagnosed. Early complete excision is recommended for hard or tenacious nodules on head, neck and upper extremity.
Adult
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Extremities
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Female
;
Hair Diseases
;
pathology
;
surgery
;
Head and Neck Neoplasms
;
pathology
;
surgery
;
Humans
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Male
;
Middle Aged
;
Pilomatrixoma
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pathology
;
surgery
;
Retrospective Studies
;
Skin Neoplasms
;
pathology
;
surgery
;
Tumor Burden
3.Preliminary study of gastric carcinoma volume measurement using computed tomography for N staging evaluation
Nan JIANG ; Shunlin GUO ; Gang WANG ; Zhonghong XIN ; Zhongchun ZHOU ; Liang YIN ; Mei YANG ; Hao YUAN ; Ying FENG
Journal of Practical Radiology 2015;(1):87-90
Objective To evaluate the value of volume measured by multi-slice spiral CT in preoperative N staging of gastric canc-er.Methods CT data of 1 93 cases of gastric cancer proven pathologically were collected and analyzed.Volume of the tumor was cal-culated in the portal phase,and the correlation between the results and N staging was evaluated.ROC curve was used to get diagnos-tic value to differentiate N stages.Results Intra-observer Kappa value was 0.77 (P < 0.05 ),0.72 (P < 0.05 ),Inter-observer Kappa value was 0.69 (P <0.05).The tumor volume data was positively correlated with different N stages (r=0.568,P <0.05). ROC curve showed that the volume could help differentiate between stage N0 and stage N1 - N3 (cutoff 12.06 cm3 ,sensitivity 55%,specificity 95%),stage N0-N1 and stage N2-N3 (cutoff 22.35 cm3 ,sensitivity 66%,specificity 86%),stage N0-N2 and stage N3 (cutoff 25.95 cm3 ,sensitivity 62%,specificity 89%)respectively.Conclusion The volume of gastric cancer measured by CT plays an important part in predicting lymph node metastasis staging and optimizing individualized clinical strategy for patients.
4.Human umbilical cord-derived mesenchymal stem cells co-cultured with hepatocytes can differentiate into hepatocyte-like cells
Hua LI ; Feng WEN ; Zhongchun QI ; Jinjun ZHOU ; Yajie ZHU ; Peng CHENG ; Dong WEI ; Xiaomei SU ; Yong TAN ; Jingjing PENG ; Qiaoli LUO ; Dong LI ; Tao ZHANG
Chinese Journal of Tissue Engineering Research 2013;(32):5772-5777
BACKGROUND:The studies have shown that the mesenchymal stem cel s derived from bone marrow and umbilical cord can be continuously cultured in vitro, and maintain the characteristics of stem cel s. The mesenchymal stem cel s can differentiate into hepatocyte-like cel s after“cocktail”induction by various cytokines. OBJECTIVE:To further identify whether umbilical cord-derived mesenchymal stem cel s in vitro co-cultured with normal hepatocytes can differentiate into hepatocyte-like cel s, and to investigate the differentiation method. METHODS:Mesenchymal stem cel s were isolated from human umbilical cord with adherent method, and the surface markers of umbilical cord-derived mesenchymal stem cel s were detected with flow cytometry. The umbilical cord-derived mesenchymal stem cel s were co-cultured with liver LO2 cel s without adding exogenous inducers. The expressions of alpha-fetoprotein, albumin and human cytokeratin 19 mRNA of hepatocyte specific markers were detected with reverse transcription PCR at 7, 14 and 21 days after culture, and periodic acid-Schiff staining was used to identify the functions. RESULTS AND CONCLUSION:Mesenchymal stem cel s could isolated from human umbilical cord successful y, showing fibroblastic morphology and adherent cel characterization. Among these cel s, 96.02%cel s were CD29 positive cel s and 96.6%cel s were CD105 positive cel s. The percentage of CD34 negative cel s was 99.65%. The percentage of CD105+CD29+double positive cel s was 94.84%. The mRNA of alpha-fetoprotein was found on the 7th day after co-cultured with LO2 cel s, and the mRNA of albumin and human cytokeratin 19 were found on the 14th day. After co-cultured for 21 days, the alpha-fetoprotein mRNA could not be observed in the co-culture group. The expressions of albumin and human cytokeratin 19 were increased at 14 days. After co-cultured for 21 days, the glycogen staining was positive. Umbilical cord-derived mesenchymal stem cel s can differentiate into hepatocyte-like cel s after co-cultured with normal hepatocytes.