Objective: Primary and secondary school students in a unique stage of development, has a unique psychological age characteristics, their mental health screening should be appropriate for their psychological development characteristics. The contents of Middle School Students Mental Health Scale (MSSMHS) were explored and revised, and the reliability and validity of the revised scale were tested. Methods: The middle school and high school students in Shiyan city of Hubei province were selected by random cluster sampling, and the revised mental health scale for middle school students was used to investigate. The 57 items of the original scale were revised, and SPSS25.0 statistical software was used for statistical analysis of the data. Cronbach's Alpha coefficient under Alpha was used to test the reliability of the scale. Factor analysis was used for structural validity analysis. Results: The results of factor analysis, correlation analysis and internal consistency coefficient showed that the revised scale had high reliability, and its validity also met the requirements of psychological measurement. Conclusion The revised content of "MSSMHS" reasonably avoids cultural taboos and sensitive words of "suicide", and has good reliability and validity, which is worth popularizing in the practice of psychological assessment of middle school students.