Objective To evaluated the clinical significance of inversely pressing method with cotton stick after extraction of infusion needle to avoid blood contamination,hemorrhage and stagnation.Methods We randomized 356 patients into the experiment group(170 cases)and the control group(186cases).In the experiment group we put the cotton stick inversely on the puncture point and press the point immediately after extraction of the needle with the left thumb.In the control group we put cotton stick on puncture point and proximal vessel after extraction.The conditions of hemorrhage and stagnation were recorded.Results The hemorrhage rate and stagnation rate in the two groups were statistically different (P<0.01).Conclusion To inversely put eotton stick on the puncture point after extraction of infusion needle could lessen hemorrhage and stagnation at the puncture point and avoid contamination.

The chemical constituents of Desmodium sambuense were studied. Chromatographic techniques were applied to isolate and purify the constituents, and the structures were identified on the basis of physico-chemical and spectroscopeic methods. Thirteen compounds were isolated from the 75% ethanol extract of Desmodium sambuens and elucidated as beta-amyrin(1), betulic acid(2), daucosterol(3), triacontanoic acid(4), lup-20(29)-en-3-one(5), tetracosanoic-2,3-dihydroxypropylester(6), stigmast-5-ene-3beta, 7alpha-ol (7),methyl phaeophorbidea(8), o-hydroxy benzoic acid(9),beta-sitosterol(10),d-catechin(11), luteolin (12), epigallocatechin (13). All of the compounds were isolated from this plant for the first time.
Fabaceae ; chemistry ; Plant Extracts ; analysis ; isolation & purification