OBJECTIVE:To study conditions of disfunction of blood clotting induced by cefoperazoned/sulbactam.METHODS:Cases of disfunction of blood clotting induced by cefoperazoned/sulbactam in our hospital from Mar to May in2004were analyzed statistically.RESULTS:The chief clinical manifestation was alimentary tract hemorrhage,which may relate to the dosage and course of treatment of cefoperazoned/sulbactam.CONCLUSION:The application of cefoperazoned/sulbactam in senile patients should be more prudent.

OBJECTIVE:To provide into the regularity of anaphylactic shock induced by fosfomycin injection.METHODS:The reports of fosfomycin-induced anaphylactic shock from 1994 to 2004 were retrieved and analyzed.RESULTS:36 cases of anaphylactic shock were publicly reported in these 10 years,among which there were 3 cases of death.CONCLUSION:Fosfomycin may induce anaphylactic shock,which should arouse more attention in clinic.

Objective:To explore androgynous female college students' personality traits,physical and psycho- logical symptom and adaptation to college life.Methods:A cluster sample of 1081 female college students was tested with the Chinese Sex Role Inventory (CSBI),NEO Personality Inventory-Revised (NEO-PI-R),Symptom Checklist 90 (SCL-90),and China College Student Adjustment Scale (CCSAS).Results:(1) The percentage of four gender role stereotypes,including masculine,feminine,androgynous and undifferentiated,were 23.8%,24.1%, 25.1%,27.1% respectively.(2) Androgynous female college students' score of extraversion,openness,agreeable- ness,conscientiousness were highest,for example,androgynous female college students' score of extraversion was (3.7?0.4),but androgynous female college students' score (2.1?0.6) of neuroticism was lowest.In contrast,un- differentiated female college students' scores of extraversion (3.1?0.5),openness,agreeableness,conscientious- ness were lowest,androgynous female college students' score (2.8?0.6) of neuroticism was highest.For other gender role stereotypes,female college students' score was between androgynous and undifferentiated.(3) As to all factors of physical and psychological symptom,androgynous female college students' scores were lowest,but undifferentiated fe- male college students' scores were highest.For example,anxiety score of androgynous female college students was (1.4?0.4),and that of undifferentiated female college students was (1.6?0.6);depression score of androgynous female college students was (1.4?0.4),and that of undifferentiated female college students was (1.6?0.6). (4) Androgynous female college students' adjustment of interpersonal relationship (37.6?5.7) was better than that of undifferentiated female college students (29.4?6.5).Other gender role stereotypes' adjustment scores of interper- sonal relationship were between androgynous and undifferentiated female college students.Conclusion:Although an- drngynous female college students' personality trait,physical and psychological symptom and adaptation to college life are better than other gender role stereotypes statistically,these results need more deeply research.

AIM:To investigate the expression of estrogen receptors in myocardium of ovariectomized female rats.METHODS:One week after bilateral ovariectomy,the female SD rats were divided into 3 groups randomly:OVX +estrogen(0.15 mg/kg,s.c.),OVX + normal sodium and control.After 4 weeks of treatment,the expression of estrogen receptors in the myocardium were evaluated by Western blotting.RESULTS:After ovariectomy,serum estrogen decreased significantly,compared with control[(88?22 vs 403?59)pmol/L,P

Objective To investigate the effects of melatonin on oxidative stress and metabolisms of glucose and lipid of diabetic patients.Methods By means of random,single-blind,placebo-parallel control clinical trial,91 of diabetic patients were randomly divided into melatonin group and placebo group.The parameters of Glutathione Peroxidase (GSH-Px),Superoxide Dismutase(SOD)and Malondialdehyde(MDA),as well as the profiles of glycemia and lipidemia were observed before and after 8 weeks treatments,respectively.Results Before treatment,both the serum levels of GSH-Px and SOD of diabetic patients were decreased significantly,whereas the serum level of MDA increased significantly.After 8 weeks consecutive melatonin treatment,the serum levels of GSH-Px and SOD increased significantly,whereas the serum level of MDA decreased significantly compared with pre-treatment in the melatonin group.There were no significant variations of serum levels of GSH,SOD and MDA observed before and after treatment in the placebo group.There were significan benefit on FBG and HbA1c after 8 weeks treatment compared with pre-treatment.The other profile of glycemia and lipidemia such as PBG,TG,TC,LDL-C and HDL-C hed no significant variations compared with pre-treatment.There were no significant variations of the profiles of glycemia and lipidemia compared with pre-treatment in the placebo group.Conclusion Melatonin significantly inhibits oxidative stress provoked by hyperglycemia and has beneficial effects on the glucose homeostasis of diabetic patients.

Objective To study the neuropsychological behavior development of preterm infants and low birth weight infants,and to provide a reference to the early prevention and intervention on developmental retardations.Methods A total of 101 preterm infants and/or low birth weight infants received the infant development test of 0 ～ 6 year-old children intelligence developmental scale for neurological development and autism behavior checklist(ABC).Results 25 boys and 5 girls suffered from different psychological mental disorders.The occurrences were as follows:10 cases with mental retardation,9 cases with the language development delay,9 cases with motor retardation,1 case with cerebral palsy and 1 case with autism spectrum disorder.The incidence of intelligence problems were that language retardation (18.9％),the fine motor (16.8％),the adapative ability (12.6％),social action (9.5 ％) and the motor delay (3.2％).There were significant differences in the scores of social communication(x2=8.88,P=0.003),adaptive ability(x2=7.41,P=0.007),the fine motor(x2 =6.22,P=0.01) and total developmental quotient(x2 =5.58,P=0.02) between city children'and rural area.The behavioral problems more consisted in self-care ability and language retardation.Conclusion Preterm infants and low birth weight infants are exposed to language,fine motor,adaptive and communication ability problems,especially the children living in country.It is necessary to improve the early education and intervention for the rural preterm infants and low birth weight infants.

Column chromatography on silica gel was used to study the chemical constituents of traditional Chinese medicine Siegesbeckia pubescens. The chemical structures of the separated compounds were elucidated by spectroscopic data analyses. As a result, eighteen compounds were obtained and identified as 3, 4'-dimethoxy quercetin(1), 3, 3', 4'-trimethoxy quercetin(2), 3, 3'-dimethoxy quercetin(3), 7, 3', 4'-trimethoxy luteolin(4), ursolic acid(5), 2β,19α-dihydroxyursolic acid(6), 2β-hydroxyursolic acid (7), stigmasterol-7-one(8), 5α, 8α-epidioxy-24(R)-methyl-cholesta-6, 22-diene-3β-ol(9), β-sitosterol(10), 2, 6-di(3-hydroxy-4-methoxyphenyl)-3, 7-dioxacyclo [3. 3. 0] octane (11), aurantiamide acetate (12), 3-(m-hydroxyl-p-methoxy)-N-(2'-p-hydroxyl-phenethyl)-2E-acrylamide(13), p-hydroxy benzaldehyde (14), m-hydroxy-p-methoxy benzaldehyde (15), 3, 4, 5-trimethoxybenzoic acid(16), monoethyl malonate(17), and p-hydroxylcinnamic acid(18). Among them, compounds 1-9, 11-18 were isolated from this plant for the first time.
Asteraceae ; chemistry ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Luteolin ; chemistry ; isolation & purification ; Medicine, Chinese Traditional ; Plants, Medicinal ; Quercetin ; chemistry ; isolation & purification ; Sitosterols ; chemistry ; isolation & purification ; Triterpenes ; chemistry ; isolation & purification

In view of risk characteristics of surgical site infection,the risk factors of the infection were graded and classified while the risk indicators system and risk assessment matrix for surgical site infection were built,based on literature analysis,integrated use of documents analysis,Delphi method and risk quantification matrix.The study indicated that surgical skills of the surgeon and surgical time length are the most important risk factors,followed by insufficient maintenance of the operating room environment and poor baseline assessment of the patient.Risk control of surgical site infection needs to focus on these key factors,optimize utilization of resources and improve the prevention and control ability of surgical site infection.

BACKGROUND:Retinoic acid is the most promising inducer for neuroblastoma minimal residual lesion, and it can induce cel differentiationin vivo, accompanied by reducing tumor cel proliferation.
OBJECTIVE:To study the effect of nanoparticle labeling on biological characteristics of neuroblastoma stem cels, and the role of 13-cis retinoic acid to induce differentiation of neuroblastoma stem cels.
METHODS:Neuroblastoma stem cels were isolated and culturedin vitro using serum-free suspension culture method, labeled with polylysine-modified γ-Fe2O3 nanoparticles and induced in culture medium containing 13-cis retinoic acid. RT-PCR was used to detect the expression of Oct-4 before and after labeling as wel as before and after induction. Immunofluorescence method was used to detect the expression of nestin before and after labeling as wel as before and after induction.
RESULTS AND CONCLUSION:Neuroblastoma stem cels were successfuly cultured in the bone marrow samples from 5 of 20 cases. Polylysine-modified γ-Fe2O3 nanoparticle labeling did no influence the viability and proliferation ability of neuroblastoma stem cels, and also had no effect on Oct-4 mRNA and nestin expression. After cultured in the culture medium containing 13-cis retinoic acid, the cel shape changed and the growth rate slowed down. Moreover, the expression of Oct-4 mRNA and nestin was gradualy reduced. These findings indicate that polylysine-modified gamma-Fe2O3 nanoparticles can be used to label neuroblastoma stem cels, and 13-cis retinoic acid can induce the differentiation of neuroblastoma stem cels.

OBJECTIVE:To explore an effective,economical drug purchasing mode for hospital drug warehouse.METHODS:The inventory costs and stock turnover rates for the zero-inventory-ordering system and fixed-interval-ordering system once applied in the Chinese patent drug stockroom in our hospital were analyzed using the analytical method of stock turnover rate.RESULTS:The average inventory costs of 2 policies were 155 600 yuan and 103 800 yuan,respectively,and their average stock turnover rates were 199.23% and 301.16%,respectively.CONCLUSION:The zero-inventory-ordering system is more conducive to the reduction of inventory cost of stock drugs.