Objective To investigate rhein on podocyte nephrin gene expression and nephrin on glomerular in diabetic nephropa-thy (DN)rats .Methods DN rats mode were made by Intraperitoneal injection of streptozotocin (STZ) 60 mg/kg once .the DN model was divided into 2 groups:rhein treatment group (A) ,model group (B) ,and in addition ,set up a control group (C) .body weight ,kidney weight ,relative kidney weight ,24-hour urinary protein excretion ,serum creatinine ,blood urea nitrogen of rats were detected after 8 weeks of intervention .kidney pathological changes were observed by using light microscope ,electron microscope . immunohistochemical technique used to nephrin protein expression .Results Both the DN rats 24 -hour urinary protein excretion and podocyte nephrin gene expression of group A decreased significantly (P<0 .05) ,improvement in renal function .Conclusion Rhein on DN kidney has a protective effect ,which may be related to its podocyte nephrin gene expression.

Objective To investigate the influence of long-term blood purification on atherosclerosis(AS) in maintenance hemodialysis(MHD) patients by measuring serum levels of homocysteine(Hcy) and carotid artery intima-medial thickness(IMT).Methods Seventy-two MHD patients visiting our hospital from June 2006 to December 2007,according to their blood purification mode,were assigned to three groups: hemodialysis(HD,n=25),hemodialysis+hemodiafiltration(HD+HDF,n=30),hemodialysis+continuous renal replacement therapy(HD+CRRT,n=17).Another 25 healthy subjects served as controls.Serum Hcy was detected before and after one-year treatment and meanwhile color Doppler ultrasonography was performed to check IMT of carotid artery and atheromatous plaques.Results The serum levels of Hcy,IL-6,MCP-1,hs-CRP in MHD patients were significantly higher than those in healthy controls(P

Objective To estimate the impact of cold wave on daily death of cerebra-cardiovascular diseases in Beijing by using case-crossover design. Methods The data of death counts was provided by Beijing Center for Disease Control and Prevention while the meteorological data was provided by Institute of Urban Meteorology,China Meteorological Administration (CMA),Beijing. The impact of six cold waves from Jan 1,1998 to Jun 30,2000 on daily death of cerebra-cardiovascular diseases were analyzed by using 1∶1 one-directional retrospective design and 1∶2 bi-directional symmetry control design. The cases were the people who dead during winter. Controls were the seventh day before or after the date of cases (or the fourteenth day). Results There were six cold waves in the investigation duration. The third cold wave in which minimum temperature declined obviously with decreasing humidity and increasing air pressure had significantly statistical significance in OR and the lag time of this cold wave was 0 day,the OR values were 1.500 (95%CI:1.032-2.181) for cardiovascular disease (CVD),respectively 1.913 (95%CI:1.066-3.432) for acute myocardial infarction,1.679 (95%CI:1.139-2.474) for cerebra-vascular disease(CBD) respectively. The other five cold waves do not have significant impact on cerebra-cardiovascular diseases. Conclusion Most cold waves studied in Beijing do not impact the daily death counts caused by cerebra-cardiovascular diseases. The cold wave with significant increased air pressure may increase the risk of death of cerebra-cardiovascular diseases without any lag time in Beijing.

Through analyzing the teaching effect,collected disagreements and suggestions by questionnaire,combining our teaching rules,we proposed efficient innovative methods and suggestions in probation of obstertrics-gynecology.

Objective To evaluate the accuracy of methods in echocardiographic measurements of right ventricular (RV) volume.Methods Forty-six healthy volunteers were examined by two dimensional echocardiography (2-DE),real time three-dimensional echocardiography (RT-3DE) and cardiac magnetic resonance imaging (cMRI) within 24 hours.2 DE adopts three methods to measure RV volume,the first is Simpson,the second is the half ellipsoid method,and the last is biplane area-length method.In RT 3DE,RV volume were calculated respectively by tri-plane method and TomTec 4D RV-function CAP software.In cMRI,RV volume was calculated by Argus software.Right ventricular end diastolic and end-systolic volume (RVEDV,RVESV) were measured respectively.Then the echo measurement were compared to cMRI.Consistency of two methods was evaluated by Bland-Altman analysis.Results ①As compared to cMRI,Simpson,the half ellipsoid method and tri-plane method underestimated RV volume,with statistical significance(P ＜0.05).Biplane area-length method and TomTec 4D RV-Function CAP had no significant statistical difference (P ＞ 0.05).② The result of correlation analysis was as follows:RV volumes from Simpson and tri-plane method had bad correlation with those from cMRI (r =0.3-0.4).RV volumes from TomTec,half ellipsoid method and biplane area length method correlated highly with those from cMRI(r =0.7-0.8).③The Bland-Altman analysis of echo methods with cMRI showed that each measurement by TomTec and biplane area-length method had a smaller limit of the agreement and a smaller difference average.Conclusions Tom-Tec 4D RV-Function CAP in RT-3DE and biplane area length method in 2-DE can measure RV volumes accurately.

Animals ; Beer ; adverse effects ; Blood Glucose ; metabolism ; Diabetes Mellitus, Experimental ; metabolism ; Lipid Metabolism ; Male ; Physical Conditioning, Animal ; physiology ; Rats ; Rats, Wistar

Objective To investigate the relationship between eosinophil and in-stent restenosis in acute coronary syndrome patients. Methods One hundred and fifity-one ACS cases werenenrolled in this study. According to the results of coronary angiography (the stented segment lumen loss over 50% was judged to be ISR), patients were divided into the restenosis group and the non-restenosis group. Results Based on the logistic analysis, no significant association was found between eosinophil and ISR, and even after adjustment of related risk factors (P > 0.05). The stratification analysis showed that the high level of eosinophil might increase the risk of ISR in ACS patients with history of hypertension (P = 0.038) and myocardial infarction (P = 0.032). Conclusion Eosinophil may be associated with the risk of ISR in ACS patients with history of hypertension and myocardial infarction. The underlying mechanisms need to be elucidated in further study.

Objective To investigate the useful parameters of transthoracic echocardiography (TTE) for the diagnosis of stroke subtypes in patients with acute cerebral infarction.Methods One hundred and one acute ischemic stroke patients met all of the following criteria including ≥50 years of age,normal sinus rhythm on admission,and transesophageal echocardiography (TEE) within 7 days from the onset.The clinical significance of the TEE parameters on admission was examined for identifying intracardiac thrombus formation as follows; left atrial dimension,left ventricular end-diastolic dimension,percentage fractional shortening,left ventricular mass index,ratio of the transmitral inflow velocities (E/A),and the deceleration time of the E wave.Results There were 28 patients with E/A ≥ 1.0 (70 ± 12) years old and 73 with E/A ＜ 1.0 (73 ± 10) years old.No patients showed pulmonary congestion on chest radiography.There were no significant differences in age,TTE parameters,and plasma levels of brain natriuretic peptide between the two groups.Patients with E/A≥ 1.0 had higher incidence of left atrial appendage (LAA)thrombus formation and/or spontaneous echograhic contrast than those with ＜ 1.0 (25.0％ vs 5.5％,x2 =7.95,P ＜0.01).A significant relationship was found between E/A and emptying flow velocity of the LAA (r =-0.569,P ＜0.01).Multivariate logistic regression analysis showed E/A was an independent predictor for LAA thrombus (risk ratio 1.531 per0.1 increase,95％ confidence interval 1.129-2.076,P =0.0002).Conclusions Increased level of E/A on admission was associated with the occurrence of left atrial appendage thrombus formation in patients with acute ischemic stroke.

Objective To explore the effect of the transtheoretical model of health behavior change on initial insulin injection in elderly patients with type 2 diabetes mellitus. Methods Thirty elderly patients with type 2 diabetes, receiving traditional training of insulin injection , were recruited as control group ( n = 30 ) between December 2013 and March 2014 . Another 30 elderly patients with type 2 diabetes, who received an education program based on the transtheoretical model of health behavior change for insulin injection training ( 30 minutes for each time and 4 times in total ) , were recruited as experiment group ( n=30 ) between April 2014 and July 2014. The knowledge of insulin injection and the operational skills in the two groups were compared between the two groups. Result Both the knowledge and operational skills at insulin injection in the experiment group were significantly better than those in the control group (P<0.01). Conclusion The transtheoretical model of health behavior change can be significant for improving the operational skills at insulin injection and therefore it can be effective in controling of blood sugar.

OBJECTIVE:To analyze the problems about the prescription review by the monitoring system for rational drug use (PASS 1.6.1.7) and discuss the countermeasures. METHODS:A retrospective method was adopted. A total of 4 942 prescriptions of our hospital in the first quarter of 2015 were selected at random,and reviewed by PASS and the pharmacist respectively. The re-view results were analyzed,and then countermeasures were put forward for the problems found to exist in the software review. RE-SULTS & CONCLUSIONS:The conformity rates of prescriptions which had been determined as acceptable through PASS review and pharmacist review were 81.20% and 97.40% respectively. PASS misjudged 929 (18.80%) rational prescriptions as irrational ones,mainly concerning administration and dosage,simultaneous existence of Western medicine and Chinese patent medicine on one prescription,repeated drug use and use with caution,etc. PASS misjudged 122(2.47%)irrational prescriptions as rational pre-scriptions,mainly concerning administration and dosage,inconformity between diagnosis and drug use,etc. The above-said prob-lem was attributed to imperfect hospital management and insufficient software intelligence. It is suggested that the hospital should strengthen the data maintenance and management of information system and PASS developer should improve software rules to in-crease the accuracy rate of prescription review.