Mesenchymal stem cells(MSCs) have became the hot spot in cell tissue engineering,cell replacement therapy,gene therapy and transplant research fields. Recent studies have shown that changes in oxygen concentrations affect many biological characteristics of MSCs. Under different oxygen concentrations,MSCs have different proliferation,differentiation,migration and chemotoxis abilities. Hypoxia is a kind of common pathophysiological status,which can promote the proliferation,apoptosis,migration and chemotoxis abilities of MSCs,while hypoxia impacts the differentiation ability depending on different cell types. The mechanism of these response might be involved in hypoxia-inducible factor-1(HIF-1) ,chemokines and their receptors,and matrix metalproteinases. Hypoxia can activate HIF-1 signaling pathway,which upgrades the expression of stromal-derived factor-1(SDF-1) ,and forms microenvironments which stem cells are adapted to and grow in. SDF-1 increases the adhesion,migration and homing of circulating CXCR4-positive progenitor cells to ischemic tissue,and promotes degradation of extracellular matrix,then enhances the migration ability of MSCs by modulating the expression of matrix metalloproteinase and its protein as well.

Objective To discuss MMP_(10) and CD_(44V6) in the thyroid carcinoma hit of give voice to and the thyroid carcinoma warlock if recur of connection.Methods The expressions of MMP_(10) and CD_(44V6) in the 69 cases thyroid carcinoma tissue were examined by appling immunochemist of technique.Results Among 69 cases thyroid carcinoma,the positive markers of expressions of MMP_(10) and CD_(44V6) were related to the thyroid carcinoma classification and the lymph gland transfering(P

Breast neoplasm shows high rate of incidence and mortality among females.Although thereare a variety of treatments for breast neoplasm,breast neoplasm metastasis is still an intractable medical problem.At present,the related mechanisms of breast neoplasm metastasis are as follows:epithelial-mesenchymal transition,dissolution of extracellular matrix,circulating tumor cells or disseminated tumor cells,tumor vessels and tumor microenvironment.Making clear of the related mechanisms of breast neoplasm metastasis has certain guiding significance for the prediction and treatmence of breast neoplasm metastasis.

Kupffer cells, the resident macrophages of the liver, play an important role in ischemia/reperfusion(L/R)injury in liver transplantation. 1schemic liver is reperfused through portal vein, and then Kupffer cells are activated. The liver is damaged by the released reactive oxygen species, inflammatory mediators and cytokines. On the other hand, upregulation of HO-1, induced by the activated kupffer cells, exerts a cytoprotective function for the IYR injury in liver transplantation models. Thus, Kupffer cells play the dual role on L/R injury in liver transplantation.

Radiotherapy is a vital treatment method for pancreatic cancer. However, the therapeutic effect of radiotherapy is significantly limited by the influence of pancreatic motion. More efficient radiotherapy for pancreatic cancer depends upon the improvement of motion management and high-quality image guidance. The emerging MR-guided radiotherapy (MRgRT) can perform functional imaging with high soft tissue resolution and no additional radiation. Multiple researches have demonstrated that MRgRT has significant advantages in terms of precise delineation of target area and organ at risk, motion management and adaptive radiotherapy, which assists radiotherapy to play a better role in the treatment of pancreatic cancer. In this paper, the application of MRgRT in pancreatic cancer was reviewed and prospected.

lschemic postconditioning(IPo)is a way that after long ischemia on liver graft,animals are given one or several brief reperfusion-ischemia before persistent reperfusion to improve the hepatic tolerance and relieve the ischemie reperfusion injury.It has been proved an effective and controlled method to attenuate the ischemic reperfusion injury.Protective mechanism of ischemic postconditioning on hepatic graft is related with protecting sinus hepaticus endotheliocyte and hepatic microcirculation,relieving hepatic cells injury and inflammatory reaction induced by oxygen free radicals,relieving calcium ovedoad in hepatic cells and mitochondria,regulating apoptosis genes,transforming ion channels condition in mitochondria.This article will makes a brief review on protective mechanism ofhepatic ischemic posteanditioning.

BACKGROUND:There was a variety of surgery treatment method in senile patients with unstable intertrochanteric fractures, such as hip replacement and internal fixation, but there was a great deal of controversy about advantages and disadvantages of different methods.
OBJECTIVE:To evaluate clinical effect and safety of total hip replacement, hemiarthroplasty and proximal femoral nail in senile patients with unstable intertrochanteric fractures.
METHODS:103 senile patients with unstable intertrochanteric fractures were selected, including 43 males and 60 females at the age of 62 to 80 years old. There were 28 cases of total hip replacement, 34 cases of hemiarthroplasty and 41 cases of proximal femoral nail. Perioperative complications and recovery of hip function after repair were compared among three groups.
RESULTS AND CONCLUSION:(1) The incidence of postoperative complications of internal medicine was higher in the proximal femoral nail group than in the total hip replacement and hemiarthroplasty groups (P < 0.05). No significant difference in the incidence of complications was found between the hemiarthroplasty and total hip replacement groups. (2) The incidence of prosthesis complications was lower in the total hip replacement group than in the hemiarthroplasty and proximal femoral nail groups (P < 0.05). No significant difference in the incidence of prosthesis complications was detectable between the total hip replacement and hemiarthroplasty groups. (3) The recovery of hip function was better in the total hip replacement and hemiarthroplasty groups than in the proximal femoral nail group during 12-month folow-up (P < 0.05), and no significant difference in the recovery of hip function was found between the total hip replacement and hemiarthroplasty groups. These results showed that total hip replacement and hemiarthroplasty had good curative effect on unstable intertrochanteric fractures in senile patients, and could effectively restore the hip function and reduce complications.

Objective To investigate the effects of dexamethasone on the growth of cervical cancer induced by HeLa cells transplanted into BALB/c mice. Methods HeLa cells were inoculated subcutaneously into BALB/c mice. Dexamethasone (15 mg?kg-1?d-1) was injected intraperitoneally from the 6th or the 16th day after tumor inoculation for 10 days continuously. Diameter of tumors was measured every two or three days. Apoptotic bodies were observed by transmission electron microscopy and counted by TUNEL. Results Dexamethasone repressed the growth of cervical cancer and induced tumor cell apoptosis, but the tumor volumes were of statistical difference between the two groups of different injection start time (P

Objective To investigate the effects of over physiological dose of dexamethasone on cervical carcinoma cell xenografted tumors in nude mouse.Methods HeLa cells were inoculated subcutaneously in BALB/c female mice aged 5-6 weeks.Over physiological dose of dexamethasone(62.5 mg?kg-1?d-1) was injected into the peritoneum from the 16th day after inoculation for 10 d.Diameters of tumor were measured every two or three days.The volumes and the inhibition rates of tumor were calculated.Apoptotic bodies were observed by transmission electron microscopy and counted by TUNEL.VEGF and TNF-? were detected by immunohistochemistry.Results In vivo,over physiological dose of dexamethasone can repress the growth of tumor and induce cell apoptosis.Compared with the control group,the tumor sizes were significantly smaller(P0.05).Conclusion Over physiological dose of dexamethasone can repress cervical tumor growth in vivo.This action may be related to apoptosis and VEGF,but have no relationship with TNF-?.

Objective To investigate the effects of intrathecal neostigmine on nitric oxide synthase (NOS) activity, nitric oxide (NO) production and cyclic guanosine 3',5' monophosphate (cGMP) content of the spinal cord in a rat model of incisional pain Methods Male SD rats weighing 250 300g were anesthetized with intraperitoneal pentobarbital sodium 40 mg?kg -1 Intrathecal(IT) catheter was implanted and the tip of the cathter reached the lumbar region, according to the method of Yaksh Incision was made in the plantar aspect of the left hindpaw under 1 4% isoflurane anesthesia, according to the method of Brennan 64 male SD rats were were divided randomly into four groups GroupⅠ received IT 0 9% NaCl 20 ?l and 30 min later inhaled 1 4% isoflurane for 5 min but no incision was made Group Ⅱ received IT 0 9% NaCl 20 ?l 30 min before incision Group Ⅲ received IT neostigmine 10?g(10?l) 30 min after incision GroupⅣreceived IT neostigmine 10?g(10?l) 30 min before incision In group Ⅲ and Ⅳ 0 9%NaCl 10 ?l was flushed through IT catheter after IT neostigmine administration A cumulative pain score was utilized to assess pain behavior The animals were decapitated 2h after incision and the spinal cord was immediately removed and lumbar enlargement of the spinal cord was dissected on ice NOS activity, NO production and cGMP content were measured by spectrophotometry and radioimmunoassy Results Cumulative pain score in group Ⅲ and group Ⅳ was significantly lower than that in groupⅡ(P