Kupffer cells, the resident macrophages of the liver, play an important role in ischemia/reperfusion(L/R)injury in liver transplantation. 1schemic liver is reperfused through portal vein, and then Kupffer cells are activated. The liver is damaged by the released reactive oxygen species, inflammatory mediators and cytokines. On the other hand, upregulation of HO-1, induced by the activated kupffer cells, exerts a cytoprotective function for the IYR injury in liver transplantation models. Thus, Kupffer cells play the dual role on L/R injury in liver transplantation.

Breast neoplasm shows high rate of incidence and mortality among females.Although thereare a variety of treatments for breast neoplasm,breast neoplasm metastasis is still an intractable medical problem.At present,the related mechanisms of breast neoplasm metastasis are as follows:epithelial-mesenchymal transition,dissolution of extracellular matrix,circulating tumor cells or disseminated tumor cells,tumor vessels and tumor microenvironment.Making clear of the related mechanisms of breast neoplasm metastasis has certain guiding significance for the prediction and treatmence of breast neoplasm metastasis.

Objective To discuss MMP_(10) and CD_(44V6) in the thyroid carcinoma hit of give voice to and the thyroid carcinoma warlock if recur of connection.Methods The expressions of MMP_(10) and CD_(44V6) in the 69 cases thyroid carcinoma tissue were examined by appling immunochemist of technique.Results Among 69 cases thyroid carcinoma,the positive markers of expressions of MMP_(10) and CD_(44V6) were related to the thyroid carcinoma classification and the lymph gland transfering(P

Mesenchymal stem cells(MSCs) have became the hot spot in cell tissue engineering,cell replacement therapy,gene therapy and transplant research fields. Recent studies have shown that changes in oxygen concentrations affect many biological characteristics of MSCs. Under different oxygen concentrations,MSCs have different proliferation,differentiation,migration and chemotoxis abilities. Hypoxia is a kind of common pathophysiological status,which can promote the proliferation,apoptosis,migration and chemotoxis abilities of MSCs,while hypoxia impacts the differentiation ability depending on different cell types. The mechanism of these response might be involved in hypoxia-inducible factor-1(HIF-1) ,chemokines and their receptors,and matrix metalproteinases. Hypoxia can activate HIF-1 signaling pathway,which upgrades the expression of stromal-derived factor-1(SDF-1) ,and forms microenvironments which stem cells are adapted to and grow in. SDF-1 increases the adhesion,migration and homing of circulating CXCR4-positive progenitor cells to ischemic tissue,and promotes degradation of extracellular matrix,then enhances the migration ability of MSCs by modulating the expression of matrix metalloproteinase and its protein as well.

Radiotherapy is a vital treatment method for pancreatic cancer. However, the therapeutic effect of radiotherapy is significantly limited by the influence of pancreatic motion. More efficient radiotherapy for pancreatic cancer depends upon the improvement of motion management and high-quality image guidance. The emerging MR-guided radiotherapy (MRgRT) can perform functional imaging with high soft tissue resolution and no additional radiation. Multiple researches have demonstrated that MRgRT has significant advantages in terms of precise delineation of target area and organ at risk, motion management and adaptive radiotherapy, which assists radiotherapy to play a better role in the treatment of pancreatic cancer. In this paper, the application of MRgRT in pancreatic cancer was reviewed and prospected.

Objective To study the effect of early intensive insulin therapy on the prognosis of critical old patients.MetlIods 76 critical old patients(＞65 years)were randomly divided into intensive insulin therapy group and control group,which were given intensive and routine insulin respectively,and biological parameters of organ func-fion and main prognostic indexes were recorded.Results The safety of intensive insulin therapy Was better.After bloed sugar was controlled strictly by intensive therapy,the incidences of aucte renal,heart and liver failure reduced obviously.There were obvious differences in mortality and the incidence of nosoeomial infection.Conclusion For critical old patients,early intensive insulin therapy can control blood glucose more effectively and promptly and im-prove the clinical curative effect.

lschemic postconditioning(IPo)is a way that after long ischemia on liver graft,animals are given one or several brief reperfusion-ischemia before persistent reperfusion to improve the hepatic tolerance and relieve the ischemie reperfusion injury.It has been proved an effective and controlled method to attenuate the ischemic reperfusion injury.Protective mechanism of ischemic postconditioning on hepatic graft is related with protecting sinus hepaticus endotheliocyte and hepatic microcirculation,relieving hepatic cells injury and inflammatory reaction induced by oxygen free radicals,relieving calcium ovedoad in hepatic cells and mitochondria,regulating apoptosis genes,transforming ion channels condition in mitochondria.This article will makes a brief review on protective mechanism ofhepatic ischemic posteanditioning.

Objective To study the preparation and quality control of Shehuang powder.Methods Medicinal materials were toasted,crushed into powder,and mixed in uniformity to make Shehuang powder.TLC was used to identify pronephrium triphyllum and rhubarb in the powder.Results TLC could identify effective reagents.The preparation method was simple and effective.Shehuang Powder prepared with this method was stable.Conclusion The compatibility of Shehuang powder was reasonable.The preparation method and quality Control was simple.

Objective To investigate the effects of intrathecal neostigmine on nitric oxide synthase (NOS) activity, nitric oxide (NO) production and cyclic guanosine 3',5' monophosphate (cGMP) content of the spinal cord in a rat model of incisional pain Methods Male SD rats weighing 250 300g were anesthetized with intraperitoneal pentobarbital sodium 40 mg?kg -1 Intrathecal(IT) catheter was implanted and the tip of the cathter reached the lumbar region, according to the method of Yaksh Incision was made in the plantar aspect of the left hindpaw under 1 4% isoflurane anesthesia, according to the method of Brennan 64 male SD rats were were divided randomly into four groups GroupⅠ received IT 0 9% NaCl 20 ?l and 30 min later inhaled 1 4% isoflurane for 5 min but no incision was made Group Ⅱ received IT 0 9% NaCl 20 ?l 30 min before incision Group Ⅲ received IT neostigmine 10?g(10?l) 30 min after incision GroupⅣreceived IT neostigmine 10?g(10?l) 30 min before incision In group Ⅲ and Ⅳ 0 9%NaCl 10 ?l was flushed through IT catheter after IT neostigmine administration A cumulative pain score was utilized to assess pain behavior The animals were decapitated 2h after incision and the spinal cord was immediately removed and lumbar enlargement of the spinal cord was dissected on ice NOS activity, NO production and cGMP content were measured by spectrophotometry and radioimmunoassy Results Cumulative pain score in group Ⅲ and group Ⅳ was significantly lower than that in groupⅡ(P

Objective To investigate the appropri ate changes of the central masking effect(CME) when there were pathologies in d ifferent segment of auditory pathway,so as to give some instructions in cli nical masking test. Methods We tested the subjects with one ear normal and th e other ear diseased. The diseased ears included conductive deafness in 30 ca ses?sensory deafness in 27 cases and retrocochlear deafness in 7 cases. Results The pure conductive deafness had very little effect on CME; secretory otistis media and chronic suppurative otistis medi a made CME lower than normal due to the secondary cochlear impairment .When su dden deafness companied with vertigo,CME appeared much higher than the normal and appeared great difference individually;but CME appeared lower than normal at other kinds of cochlear deafness ears.CME appeared differently at retrocochlear deafness ears. Conclusion Audiometry results should be corrected when th e masker higher than 40 dB EM,because the masker can make the CME higher than 5 dB in the conductive impairment ears.In the sudden de afness companied with vertigo ears,CMEs were too high and too individually to fi nd a suitable correction for PTT.Insert earphone should be used in measuring the air-conduction PTT,and bone-conduction PTT test might be given up.