OBJECTIVE: To establish the quality standard of Codonopsis lanceolata.METHODS: C.lanceolata was identified by TLC and the content of ginsenoside in C.lanceolata was determined by HPLC.RESULTS: The TLC spots of syringin and echinocystic acid were clear and easy to identify.The linear range of ginsenoside in C.lanceolata were 2.176～21.76 ?g (r=0.999 8) with an average recovery of 96.54%(RSD=1.2%,n=9).CONCLUSION: Established quality standard can be used for the quality control of C.lanceolata.

The architecture and environment of hospital are closely linked with the health of patients,which are of important ethical meaning.The layout,ward,waiting room,planting and decoration of a hospital should be centered on patients-oriented value to promote people's health.

Objective To investigate the effect of rosiglitazone on the expression of platelet CD40 ligand (CD40L) in insulin-resistant rats, and to further determine the relationship between CD40L and insulin resistance. Methods 60 healthy male SD rats [(200±20)g] were randomly divided into 4 groups: control group (C), high fat group (HF), low dose rosiglitazone group (LR) and high dose rosiglitazone group (HR). Rats in group C were fed normal chow diet, and the others were given high fat chow diet. After 12 weeks, high dose of rosiglitazone (10mg/kg) was given to rats in group HR and low dose of rosiglitazone (5 mg/kg) was given to rats in group LR for 4 weeks. Rats in group HF and group C were given 0.9% sodium chloride solution. The level of sCD40L was measured by ELISA and the expression of platelet membrane CD40L was detected by immunoprecipitation and Western blot. The insulin resistance (IR) index was calculated by homeostasis model assessment (HOMA). Results HOMA-IR, sCD40L level and platelet membrane CD40L expression were higer in group HF than in group C (9.8±3.2 vs. 5.9±1.7, 367.3 ±35.3 vs. 232.3±120.6, 2.1±0.4 vs. 1.4±0.2, respectively, all P<0.05). Compared with the group HF, HOMA-IR, sCD40L level and platelet membrane CD40L expression were obviously decreased in group HR(5.4±1.1, 276.9±54.0, 1.4±0.3, respectively, all P<0.05), and there were no significant differences in HOMA-IR, sCD40L level and platelet membrane CD40L expression between group HF and group LR (P>0.05). Conclusions In insulin-resistant rats, the level of sCD40L and the expression of platelet membrane CD40L were higher. After treatment with high dose of rosiglitazone, sCD40L level and platelet membrane CD40L expression were decreased with the improvement of insulin resistance.

Objective To investigate the prognosis and its related factors of acute promyelocytic leukemia(APL).Methods Fifty-four patients with APL,from January 1994 to February 2005 in our hospital,were studied retrospectively.Univariate analysis of the potential factors influencing survival of these patients was carried out by comparison of the cases in same stage with chi square test and Log-Rank method.Prognostic indicators were demonstrated by CR rate,overall survival(OS)and event-free survival(EFS).Results 45 patients(83.3%)entered CR.The results showed that 1-year and 3-year OS of 54 patients were 81.5% and 73.3% respectively,1-year and 3-year EFS were 78.4% and 66.6% respectively.The estimated 5-year OS and EFS were 60.5?2.4% and 52.4?1.5% respectively.The analysis of prognostic factors showed that:Sex,age,initial WBC count,initial PLT count,marrow cellular morphology features,first induction regimen and time from induction therapy to CR were all important prognostic factors of APL.Conclusion Factors,including age

AIM:To analyze and compare the chemical constituents difference between single medicine decoction(SMD) and mixed medicine decoction(MMD) of Changweikang Granules. METHODS: TLC and HPLC methods were applied to comparing the chemical constituents in SMD and MMD of Changweikang. RESULTS: Spots of TLC between SMD and MMD similar,except for some difference in the HPLC. CONCLUSION: The analyses were carried out for the first time.We found that the plus of the chemical constituents in SMD equal to MMD of Changweikang,also during the course of MMD,new constituents may be generated.Based on these,we proposed that the material base of Changweikang were the rational preparation and complementation of the effective components and the generation of new constituents.

Bidirectional evaluation method is established for Standardized management of instructing & teaching of Radiology Technology. Four-year experience shows that it does good to the improvement of the teacher and the student.

Objective To evaluate performance of the AVE‐766 automated urine sediment analyzer(AVE‐766) based on the ma‐chine vision for detecting erythrocytes(RBCs) ,leukocytes(WBCs) ,epithelial cells(ECs)and CASTs in urine specimen .Methods The within‐run variable coefficients(CVs) ,linearities and carryover rates for RBC ,WBC ,EC ,and CAST in urine specimens detected by using the AVE‐766 were analyzed ,the results of RBC ,WBC ,EC ,and CAST count in urine specimens detected by using AVE‐766 and Fast‐Read102 counting plate(Fast‐Read102) were compared .Results The within‐run CVs for RBC ,WBC ,EC ,and CAST detected by using AVE‐766 and Fast‐Read102 were increased by decreases of concentration of urine sediment .Good linearities (R2 >0 .97 ,P<0 .05) were observed for RBC(in the range of 60 -1 255/μL) ,WBC(in the range of 68 -2 718/μL) ,EC(in the range of 28-296/μL) and CAST(in the range of 5-86/μL) detected by using the AVE‐766 .The carryover rates for RBC ,WBC , EC ,and CAST detected by using AVE‐766 was 0 .9% or less .The values detected by using AVE‐766 were correlated well with those detected by using Fast‐Read102 for RBC ,WBC ,and EC in urine specimens(0 .67< r<0 .75) .However ,for CAST ,the values detected by using AVE‐766 were poorly correlated with those detected by using Fast‐Read102(r=0 .183) .There were statistically significant differences between manual and automated urinalysis for RBC ,WBC ,EC ,and CAST in urine specimens(P<0 .05) .Con‐clusion The AVE‐766 could not take over microscopic examination ,only is suitable for the first screening to detect RBC ,WBC , EC ,and CAST in urine specimen .

Purpose:To investigate the relationship between HELICOBACTER PYLORI (Hp) and gastric carcinoma, as well as the possible carcinogenic mechanism by Hp. Methods: DNEL technique and immunohistochemical staining were used in the research to study the state of apoptosis, proliferation and p53 gene expression. Total 100 gastric mucosal biopsy specimens, including normal mucosa, Hp negative and Hp positive gastric precancerous lesions as well as gastric carcinomas were studied. Results:There were several apoptotic cells in the superficial epithelium and a few proliferative cells within neck of gastric glands, and no p53 protein expression in normal mucosa. In gastric carcinoma, apoptotic cells accounted for 1.62%, while proliferative cells 4.99%, and P53 protein significantly increased. in metaplasia, the apoptotic index (AI), proliferative index (PI) and positivity of p53 expression in Hp positive group were higher than those in normal mucosa ( P

Objective To reveal the change tendency of serum osteopontin(OPN) in carcinogenesis and development of gastric cancer(GC), and explore the usefulness of serum OPN in the diagnosis of GC and the differential diagnosis of early GC and advanced GC.Method Serum OPN levels were detected by enzyme-linked immunosorbent assay, including 70 advanced GC, 24 early GC, 47 dysplasia, 42 intestinal metaplasia, 46 chronic superficial gastritis patients and 38 normal controls.Results Serum OPN level in GC group (57.03 ng/ml) was significantly higher than those in other groups.Serum OPN level in advanced GC group (66.05 ng/ml) was significantly higher than that in early GC group (41.93 ng/ml), serum OPN level in early GC group was significantly higher than those in dysplasia, intestinal metaplasia and chronic superficial gastritis, and serum OPN level in the latter three groups was significantly lower than that in normal controls (P