2.Establishment and Application of Information System for Hospital Infection Management
Chinese Journal of Nosocomiology 2006;0(02):-
OBJECTIVE To establish and apply the hospital infection information system,and study the risk factors and occurrence pattern hospital infection,to control the incidence of hospital infection.METHODS The hospital infection management software was developed by the hospital with the Hope-Bridge Technology Company of Guangzhou.RESULTS This system can joint with the hospital information system(HIS) seamlessly,as a subsystem of HIS,and be utilized in the real-time monitoring for all inpatients.It has the function of inquiry,statistics and analysis,it can monitor correctly and comprehensively on infection case,hospital environmental hygiene,usage of antibiotics and intervening operations.CONCLUSIONS The hospital infection management software is convenient and easy to operate,which can enhance the efficiency and effect of the management of hospital infection.
5.Primary culture of human vascular smooth muscle cells and their calcification mode
Xiao-zhong, CHENG ; Ting, SONG ; Bei, HUANG ; Hui, ZHONG
Bulletin of The Academy of Military Medical Sciences 2010;34(1):37-39
Objective To establish a calcification mode in vitro of human vascular smooth muscle cells (HVSMCs) induced by β-GP. Methods Primary HVSMCs were obtained from human embryo by plant method and confirmed by stain with α-sm-actin antibody. The cells after 4-6 passages were divided into two groups.The control group was incubated with normal DMEM medium while the calcification group was incubated with the medium containing 10 mmol/L β-glycerophosphate for 10 days.Calcification was confirmed by alizarin red S stain and alkaline phosphatase(ALP) assays. Results The primary cells observed by S-P stain were positive and the cells after being stained were pale yellow. After being induced with β-GP, the cells of calcification group began concentric growth and formed vesicles. Alizarin red S staining showed that the reaction of calcifying nodules was red,ALP activity was higher than that of controls at various time points(4 d,6 d,8 d and 10 d,P<0.01 ).Conclusion The HVSMCs could be induced into calcification in vitro by β-GP, and this model contribates to further studies of vascular diseases.
6.The significance of TFPI test in Pre-DIC state of rats
Zhong YUAN ; Xichun XIAO ; Min YANG
Journal of Chinese Physician 2008;10(4):487-488
Objective This pre-DIC rat models were constructed by injecting LPS in order to reveal the relationship and significance among tissue factor pathway inhibitor (TFPI),platelet, fibrinogen, prothrombin time and activated partial thromboplastin time. Methods Enzyme-labeled immunosorbent assay was used to detect blood plasma TFPI in pre-DIC state rats(n=16),DIC state rats(n=8)and health rats(n=8).Platelet, fibrinogen, prothrombin time and activated partial thromboplastin time in the same rat were detected. Results TFPI increased first(pre-DIC state)and then decreased(DIC state)in the blood plasma of rata, but the levels of TFPI were always higher than that in the normal state. Conclusions TFPI is more sensitive and accurate to monitor the pro-DIC state than routine examination, which is very useful in the early diagnosis of pre-DIC state.
7.A pulse sequence eliminating zipper artifact of MRI
Youlin PENG ; Rong XIAO ; Dafeng ZHONG
Chinese Journal of Tissue Engineering Research 2010;14(4):649-652
BACKGROUND: Various artifacts are found to be appeared in the nuclear magnetic resonance; the frequently appearing ones are the motion artifacts, plait artifacts, metallic artifacts, partial volume effect artifacts, chemical shift artifacts, phase artifacts, zipper artifacts and so on. However, there is still has no report on the controlling and revising method of the zipper artifacts which is produced in the spin-echo sequence imaging process. OBJECTIVE: Based on the discussion of the mechanism of the production of zipper artifact in the spin-echo sequence imaging process, a new impulse sequence is worked out to avoid the zipper artifact. METHODS: Standard SE sequence was complied to obtain the standard image, and compiling the artifact sequence to obtain the linear disturbance image and zipper artifact image, compiling the modified sequence to obtain the modified zipper artifact image. RESULTS AND CONCLUSION: Usually, the linear disturbance image could obtained in the instantaneous gradient of 5 degree with the echo sequence of 120 degree, and the zipper artifact will be obtained in the instantaneous gradient of 90 degree with the echo sequence of 120 degree. Modified sequence instantaneous gradient of 90 degree with the echo sequence of 120 degree can completely eliminate zipper artifact. The interesting result obtained in this paper is that the zipper artifact can be totally avoided with this new impulse sequence.
8.Research on cortical stimulation for treatment of stroke
Hongxin GUAN ; Yunshi XIAO ; Jun ZHONG
Journal of Shanghai Jiaotong University(medical Science) 2010;30(1):108-111
Stroke is characterized by a group of acute cerebral vascular diseases which attack acutely with focal neurological deficits. Residual motor deficits often sojourn after stroke. Cortical stimulation, which is a technique developed many decades ago, has recently re-emerged as a promising method for researchers in their quest to causally probe cortical representations of sensorimotor and cognitive functions and to facilitate the treatment of various neuropsychiatric disorders. The article summarizes the research progress of cortical stimulation in the promotion of motor function recovery after stroke, the method of operation, the possible mechanisms and the prospect.