In the present work, the bioavailability of heme iron has been studied by means of the hemoglobin regeneration method with iron-deficient rats model, which was conducted by feeding a low iron basal diet. Anemic rats were then divided into groups to which basal diets with various iron levels (7, 14, 28 ppm Fe) from heme iron (Group 1,2,3) and ferrous sulfate (Group 4, 5, 6, as a reference standard) were subsequently fed respectively.After 5-week regeneration period, the regenerated hemoglobin levels of the rats of corresponding groups were (1) 9.56→12.25, (2) 9.71→12.02, (3)9.51→ 11.09, (4) 9.74→10.81, (5) 9.66→11.24, and (6) 9.67→12.05, thereby the percentage of relative bioavailability (RBA) of heme iron were 117.7%, 124.8%, and 94.9% respectively. This result showed that the lower level of heme iron has a higher RBA. The reason why the effect of high level of heme iron was relatively lower was not fully understood.

Objective To introduce the clinical application of Tono-Pen tonometer.Methods The intraocular pressure was detected with Tono-Pen XL tonometer at different time points for sixteen patients went through laparoscopic gynecologic operation,then the statistical analysis of the results was performed.Results In processes of artificial pneumoperitoneum and low position,the intraocular pressure escalated gradually,and after artificial pneumoperitoneum,the intraocular pressure descended rapidly.Conclusion Tono-Pen tonometer is the only tonometer can be used for the patient of general anesthesia until now.

Objective To evaluate the effect of OTD(observation-teaching-discussion) method in teaching of kinesiology of rehabilitation medicine.Methods 110 students majoring in rehabilitation medicine were divided into two groups,routine teaching group(56 students) and OTD teaching group(54 students),and the teaching effect was evaluated by exam score and questionnaire.Results There were obvious difference in the exam score and questionnaire between two groups(P<0.01).Conclusion In the teaching of medical rehabilitation,OTD method is more advantageous than routine method in bring up students' study interest,the initiative spirit,and the ability of self-study and analyzing.

Objective To investigate the methods of treating great saphenous varicosity by inserting optical fiber straight into EVLT.Methods Wuhan Linyun Laser Therapeutic System was introduced to treat 205 cases (257 limbs)of great saphenous varicosity.Optical fiber instead of blood vessel sheath and catheter was inserted straight into endovenous laser treatment(EVLT)through arteriovenons permanent needle was adopted.Results Operations lasted 35～55 minutes,averaging 45 minutes.Patients were hospitalized for 5～7 days.Postoperative vis- its were followed for 3～12 months,averaging 6 months.Two months after the operation,appraisal according to the curative effect evaluation criteria was carried on.The cure rate for superficial varicosity is 97.3%(250/257),for lower extremities swelling asthenia is 93.7%(150/160),for skin pigmentation is 91.8%(56/61),and for swelling is 90.9%(20/22).10 cases(3.9%)suffered from skin ambustion.9 cases(3.5%)suffered from shank anesthesia and stabbing pain.15 cases(5.8%)suffered from hypodermic tubercle.The patients had not deep venous thrombosis after the operation and the wounds healed well.Conclusion Inserting optical fiber straight into EVLT through arte- riovenous permanent needle brings about more accurate treatment.It can be operated simply,causing small wounds and less pain.

Acupuncture Therapy ; Female ; Humans ; Middle Aged ; Syringomyelia ; therapy

Objective: To study the effects of MMP-9 (Matrix Metalloproteinase-9, MMP-9) in the pathogenesis of abdominal aortic aneurysms (AAAs) by localizing the expression of MMP-9 in the aneurysmal tissues. Methods: By means of immonohistochemistry, the frozen sections (5 μm) with aneurysmal tissues (n = 10) were incubated with MMP-9 antibody-added agents, then the sections were stained and observed under the microscope to localize the expression of MMP-9, which displayed a brown precipitate within the arterial walls. The normal arterial wall tissues (n = 10) and the diseased arterial wall tissues from the arterial occlusive diseases (AODs) (n = 15) were also immunized exactly the same way as control. Results: A quantity of positive granules which appeared within the aortic media showed the strong expression of MMP-9 in the AAAs, with the positive rate reaching 95%(19/20), while no expression of MMP-9 was observed in the normal artery. However, the scattered distributed positive granules were seen within the arterial wall of some cases of the AODs, implying the weak positive expression of MMP-9 in this disease with the positive rate of 26.7% (4/15). There was a significant difference of the expression of MMP-9 within the arterial wall between the AAAs and AODs(P<0.01). Conclusion: High expression of MMP-9 within the aortic media faciliatates the degradation of collagen and elastin fibres and subsequent dilation of the aortic artery , thus playing an important role in the pathogenesis of AAAs. To refrain MMP-9 from enhanced expressing within the aortic wall is of clinical significance in the prevention and treatment of AAAs.

Adult ; Biomarkers, Tumor ; metabolism ; Epididymis ; metabolism ; pathology ; surgery ; Humans ; Inhibins ; metabolism ; Leydig Cell Tumor ; metabolism ; pathology ; surgery ; Male ; Testicular Neoplasms ; metabolism ; pathology ; surgery ; Vimentin ; metabolism

Objective　The clinical manifestations and mechanism of pathological synkinesia were studied. Methods　Recurrent pathological synkinesia was measured by clinical and experimental nictitation reflex. The changes of nerve-muscle junctions were observed via scanning electron microscope. Results　All the 32 cases of pathological synkinesia were denervation of degree Ⅲ and severe facial palsy. Reaction undulatory forms of musculus orbicularis oculi and musculus orbicularis oris recorded simultaneously at the stimulated side in trigemial-facial nerve reflex test accounted for 81%(28/32) patients and 89% guinea pigs. Conclusion　Pathological synkinesia was the marker of severe facial palsy and was related with over-error in regeneration of nerve.

Objective To compare the influences of general anesthesia and intra spinal anesthesia on circulation, respiration, body temperature and anesthesia-related complications in patients undergoing percutaneous nephrolithotomy (PCNL), and assess the effectiveness and safety of both anesthesia. Methods Forty ASA Ⅰ - Ⅱ patients elective for PCNL surgery were divided into two groups by random digits table with 20 cases each:group Ⅰ (endotracheal general anesthesia) and group Ⅱ (intra spinal anesthesia). The temperature, heart rate (HR), mean arterial pressure (MAP), pulse oxygen saturation (SpO2) values and postoperative shivering, nausea, vomiting, back discomfort and the incidence of sore throat were observed and recorded. Results The anesthesia was stable, there were no changes in MAP, HR at different time in group Ⅰ . The anesthesia in group Ⅱ was effective, MAP at 15 min after anesthesia,and HR,MAP after lithotomy position and prone position were obviously changed in group Ⅱ compared with those before anesthesia and group Ⅰ (P ＜ 0.05 ). The temperature at 30,60,90, 120 min after anesthesia decreased compared with that before anesthesia in two groups (P ＜ 0.05 ), and the temperature at 30,60 min after anesthesia in group Ⅰ [(35.8 ±0.6), (34.8 ± 0.5)℃] was lower than that in group Ⅱ [(36.2 ± 0.6),(35.6 ± 0.5)℃](P＜ 0.05).During recovery,complications such as shivering, nausea,sore throat, back discomfort occurred to some extent, of which the incidence of shivering was the highest. Conclusion Both of two anesthesia are applicable to PCNL. When intra spinal anesthesia is used,the life indicators of patients need to be observed and general anesthesia is preferable for the obesity,less physical and the old with poorly compensatory function.

Guided bone regeneration technique (GBR) is regarded as one of the most important methods to increase the bone amount of alveolar ridge, which is often recommended to be applied in the dental implantation surgery. Barrier membrane is utilized in GBR technique to cover the bone defect and create a secluded space, which prevents the connective tissue from growing into the space and facilitates the growth priority of bone tissue. In recent years, along with the emerging of different kinds of barrier membranes and bone grafting materials, the scope of clinical application of this technology is continuously expanded. This article reviews the classification and property of the membranes and the application situation of guided bone regeneration technology in the field of dental implantation.