The mechanism of complex unfolding process of Pseudomonas aeruginosa apoazurin is an arguing problem. Recent published results indicated that this problem could be resolved by hypothesizing two native conformations coexisting in solution.Urea-induced unfolding of apoazurin was investigated further using intrinsinc fluorescence and CD spectra. Equilibrium unfolding curves in urea could be depicted with an apparent two-state transition, but a biphase kinetic process. The fast unfolding process is finished within a few seconds as monitored by stopped-flow fluorescence intensity, whereas the slow process requires several hours for as well as the circular dichroism spectra just after manual mixing of protein and denaturant could be simulated by superposition of the spectra of the native and fully unfolded protein with the same coefficient of 0.37. This strongly suggests the three-state mechanism with a partially unfolded intermediate on its pathway is inadequate. The hypothesis of two native conformations coexistence is a reasonable selection.

Taking into account the near infrared spectra(NIR) on numerous predictor variables with serious collinearity and having nonlinear quantitative relationship with the chemical compositions, a novel nonlinear partial least squares(PLS) approach, termed as Spline-PPLS, was constructed by combining the penalized partial least squares(PPLS) regression with B-splines transformation. Firstly, the observed spectral predictors were considered as discrete observations of curves of the wavelength and were nonlinearly transformed using B-spline basis functions. The choice of the degree of the polynomial pieces and of the number of knots was performed using the cross-validation strategy. Then, the PPLS algorithm was performed on the high dimensional transformed data matrix to build the calibration model by imposing a penalty term to the optimization criterion of PLS. The roughness penalty term indeed controlled the curvature of the functions and its smoothing parameter could also be obtained by the cross-validation. Finally, the proposed Spline-PPLS approach was applied to the wheat NIR diffuse reflectance spectra reconstruction and quantitative analysis. The result indicates that the Spline-PPLS approach not only can yield high accuracy reconstructing spectrum, but also improves the model prediction accuracy in the case of nonlinear relationships.

Objective We discussed the application and clinical effect of serf nursing education in patients with infectious tuberculosis. Methods We divided 140 patients with infectious tuberculosis into the test group and the control group with 70 cases in each group. The control group adopted health education. The test group received individual and selective self nursing education, including serf mastering of tuberculosis, self monitoring of illness, completion of ther therapeutic process by oneself, serf management of diet, self regulation andf control of motion and education of self nursing technique. The intervention continued for 3 months. Pulmonary tuberculosis specific knowledge inventory, therapeutic compliance investigation inventory and general questionnaire on living (GQOL) were adopted in the two groups and the results were compared. Results The therapeutic compliance and life quality of the test group were evidently higher than those of the control group after self nursing education (P<0.01). The mastering of tuberculosis knowledge of the test group was also superior than that of the control group (P<0.01). Conclusions Self nursing education could increase the therapeutic compliance as well as the life quality of patients.

Objective To investigate the means which can prevent postoperative incision infection of elderly patients with obstructive colorectal cancer. Methods Sixty-seven cases of elderly patients with obstructive colorectal cancer adopting surgical treatment were divided into two groups, including experimental group with 37 cases and control group with 30 cases. Put self-made negative pressure drainage tube with side-hole into subcutaneous interspace of abdominal incision of experimental group alter operation, kept continuous negative pressure drainage 7-10 d and pull out the self-made subcutaneous negative pressure drainage tubes till drainage volume decreased obviously. The abdominal wall incisions of the control group were given conventional suture. Results Incision infection occurred in only one case (2.7%) and no incision dehiscence occurred in the experimental group, and incision healing time was (9.36 ± 2.18) d. Incision infection occurred in 7 cases (23.3%) in the control group, of which there were 3 cases (10.0%) of wound dehiscence, incision healing time was (12.25 ±4.06) d. Incision infection rate of the two groups had significant difference (χ2= 6.706,P < 0.05), incision dehiscence rate of the two groups had no significant difference (P 0.05), incision healing time of the two groups had significant difference (t = 6.676, P < 0.05). Conclusion Using the self-made subcutaneous negative pressure drainage tube and keeping it continuous negative pressure drainage after operation can be effective in preventing incision infection of elderly patients with obstructive colorectal cancer, and promote wound healing.

Objective] To sum up the clinical experience of professor Zhang Qin in treating menostaxis caused by PCSD. [Methods] From the TCM theory , therapy and successful case,it analyses the professor Zhang Qin's unique feature of PCSD.[Results] Professor Zhang considers that the disease is induced by the blades,which leads to blood stagnation-heat and Qi-deficiency,and puts forward the two-step traditional Chinese medicine: step one is to expel blood stasis using modified Jiawei Shixiaosan in the menstrual cycle from the first day to the fifth day ,step two is to stanch bleeding using modified Guchongtang in the menstrual cycle from the sixth day to the twelfth day. A case in point proves that two-step traditional Chinese medicine can recover the menstrual period normally. [Conclusion] Professor Zhang Qin's clinical experience in treating menostaxis caused by PCSD obtains the good curative effect,having enlightenment meaning to clinical practice,with promotion value.

Objective To analyse effect of Danhong injection on serum levels of bone markers and healing time in postoperative patients with tibial fractures.Methods 52 patients who were diagnosed with tibial fractures in our hospital were collected and randomly divided into control group and experimental group.All patients were treated with intramedullary nailing.After the operation, on the basis of routine treatment, patients in control group were treated with ossotide injection 50 mg, dissolved in 250 mL 0.9% sodium chloride solution, one times per day.Patients in experimental group was treated with Danhong injection 20 mL, dissolved in 250 mL 0.9% sodium chloride solution, one times per day.14 days for one period of treatment, two groups were treated for two cycles.After the treatment,the serum levels of bone Gla-protein (BGP), carboxyterminal propeptide of typeⅠprocollagen (PICP), bone alkaline phosphatase (BALP) and the racture healing time were detected in all patients.Results Compared with control group post-treatment, the serum BGP and PICP levels were higher in experimental group (P<0.05);the serum BALP level was higher in experimental group (P<0.05); the average fracture healing time of the patients in experimental group was shorter (P<0.05).Conclusion Danhong injection can significantly increase the serum BGP, PICP and BALP level in postoperative patients with tibial fractures, shorten fracture healing time.

Objective:Dual-track teaching mode in higher colleges and universities of Chinese medicine in clinical pathology teaching reform and practice, so as to change the phenomenon of university students of traditional Chinese medicine western medicine foundation weak, and improve the teaching quality of clinical pathology.Methods: We use the traditional teaching method (LBL) and teaching method of the problem-based learning (PBL) to make double-track recess internship teaching model in clinical pathology, and in practice use the access type, interactive type and potential mining methods to motivate the students’ learning.Results: The dual-track teaching model realizes complementary teaching effect of a variety of teaching methods, makes the teaching quality enhance unceasingly. It not only exercises the students’ ability of autonomous learning, cultivate the students’ clinical thinking, but also stimulates students’ imagination and improves the students’ ability to analyze and solve practical problems.Conclusion: The dual-track teaching mode is worth popularizing in the higher colleges and universities of traditional Chinese medicine, and it can improve the students’ comprehensive quality and innovation and make it better from students to the role of the doctor.

Objective To explore the effects ofDoctor-nurse Integratingmode on the operation room nursing quality improvement. Methods From July 2014 to Dec. 2014,11 423 operations in our hospital were chosen as control group,while 10 897 operations from Jan. 2015 to June 2015 as intervention group.An administrating group of Doctor-nurse Integrating mode was formed, with both doctors and nurses acting as quality controllers. A144 Regular Meetings System was also formed. The author retrospected and analyzed 11 423 negative accidents which occurred during operation. Management details and responsibilities were specified, and training was enhanced. All these were monitored, controlled, implemented and improved by operation doctors, anaesthesia doctors and operation room nurses. Results The rate of negative accidents dropped from 1.226%(140/11 423) to 0.340%(37/10 897) since the implementation of Doctor-nurse Integrating mode of operation quality control. Conclusions Doctor-nurse Integrating mode of operation quality control has positive effects on the improvement of operation nursing quality.

OBJECTIVE:To study the preparation of fluconazole eyedrops as well as its quality control.METHODS:The content of fluconazole in eyedrops was determined with HPLC.The quality control of eyedrops has done in accordance with ChP.RESULTS:The average recovery was100.4%,RSD=0.54%(n=5).CONCLUSION:The formula of this preparation is reasonable with simple and convenient technology.Moreover,its quality control is easy to handle.

Objective To explore the effect of glucocorticoid on disbalance of the Th 1/Th 2 cytokines to investigate the mechanism of treatment of breathing in glucocorticoid in asthmatic patients. Methods The peripheral blood was drawn and the plasma IL-4,IL-12 and IgE of control group and patients were detected by ELISA method before and after glucocorticoid treatment. The results were analysed statistically. Results ⑴In asthmatic group, plasma IL-4 and IgE levels, and PEF variant rate after treatment were lower than those before treatment (P0.05); and ⑷ Correlation analysis showed that IL-4 level positively correlated with the IgE level and PEF variant rate(r=0.64,r=0.52;P