MicroRNA-451 (miR-451) is significantly lowly expressed in glioma,gastric cancer,colorectal cancer,liver cancer and other tumor tissues.Upregulation of miR-451 can significantly inhibit tumor cell proliferation,invasion and metastasis as well as increasing tumor cell apoptosis and improving radiotherapy and chemotherapy sensitivity and other biological effects.

Autoantibodies are useful laboratory parameters for diagnosis of autoimmune diseases (AID).Methods for the detection of autoantibodies have achieved quantitative or semi-quantitative to some extent.Furthermore,with the development of detection technology,high-throughout and quantitative technology has been the trend in autoantibody measurement.Compared with qualitative results,the quantitative ones may provide more values for diagnosis,prediction,prognosis and therapeutic monitoring for AID.Therefore,although quantitative technology of autoantibodies is still faced a number of challenges,the detection for autoantibodies has come into the era of quantitation.

Objective To analyze the mental health status and personality characteristic of hyperplasia of breast,so as to provide sound scientific basis for enhancing the quality of life of those patients.Methods Assessments were made by using Symptoms Checklist 90 (SCL-90)and 16 Personality Factor Questionnaire (16-PF) for the mammary gland hyperplasia patients and the normal,and data were analyzed with SAS 9.2.Results (1)Significant differences were found in the scores of somatization,compulsion,sensitivity,depression,terror and paranoid between the byperplasia of breast and heahhy subjects.(2)Multivariate and unconditional Logistic regression analysis revealed that the protective factor of psychosomatic symptoms is the warmth stability and perfectionism which factor regression coefficient was negative; the risk factor of psychosomatic symptoms was the dominance,suspicion,apprehension and tension which factor regression coefficient was negative.Conclusions There are various psychological problems which affected by personality characteristic in the patients with breast hyperplasia,treatment should be optimized based on the personality characteristics of patients with breast hyperplasia,including changing negative attribution model,disseminating preventive knowledge,and finally improve the mental health condition of patients with breast hyperplasia.

Objective To study the dilution model of the early pregnancy test strip for the quantitative detection of serumβ-hu-man chorionic gonadotropin(β-hCG)level by the Abbot AxSYM detection system and its application.Methods The detection re-sults of serumβ-hCG level in 5 612 outpatients and inpatients of the internal medicine department and the gynecology and obstetrics department during 5 years were retrospectively analyzed.In addtion,234 serum samples from the patients were collected for prelimi-narily determining the serumβ-hCG level by the early regnancy test strip and then the quantitative detection of serumβ-hCG level was detected by the Abbot AxSYM detection system.Results The retrospective analysis showed that 241 cases(4.6%)were low-age pregnant women.The qualitative screening was necessary.The screening model was successfully established.But one thing to note was that the part of serum sample needed to be taken out and put into another tube for determination.Conclusion Establishing the rational and effective dilution interpretation model of early pregnancy test strip for screening serumβ-hCG can bring the econom-ic and time benifits for the routine detection work of the Abbot AxSYM detection system.

Objective To investigate the role of PPAR? in the pathogenesis of colorectal cancer. Methods Literatures about PPAR? and the pathogenesis of colorectal cancer were reviewed and analyzed.Results PPAR? is expressed in the nucleuses of glandular epithelia lining colon and rectum. It is normally suppressed by wild-type adenomatous polyposis coli (APC) but is up-regulated by enhanced ?-catenin/T cell factor-4 (TCF-4) binding to TCF-4-responsive elements in the PPAR promoter when an inactivating APC mutation occurs, which indicates PPAR is a potential downstream target of the APC/?-catenin/TCF-4 signaling pathway in colorectal cancer. Consistent with PPAR’s role as an APC/?-catenin/TCF-4 target, some studies reported that PPAR mRNA is frequently overexpressed in colorectal cancers of both humans and rodent animals, which indicates that PPAR is relevant to the tumorigenesis of colorectal cancer. Conclusion PPAR? is closely related with the pathogenesis of colorectal cancer.

Burns ; therapy ; Fluid Therapy ; Humans

Objective To analyze the distribution and drug resistance of Escherichia coli which separated from clinical.Methods Collecting the bacteria specimens to separating and training Escherichia coli,and then screening and monitoring drug susceptibility by The Dark Horse Microbial Analysis System,from January 2010 to July 2011.Results The Escherichia coli 486 strains which separated from clinical,the male 297 cases,female 189 cases,secretion separation 236 strains was the highest rate (48.56％),sputum 165 strains (33.95％),urine 39 strains (8.02％),and the rest of the sample 46 strains (9.47％).ESBLs 198 strains,which resistance in 19 antibiotics were higher than Escherichia coli,and it was multiple antibiotic resistance such as Aminoglycoside,Quinolone etc; Escherichia coli strains were sensitive to imipenem.Conclusion Escherichia coli which separated in surgery,respiratory tract,and urinary had a large proportion,and Resistance of Escherichia coli was serious,we should according to the Drug sensitivity of Escherichia coli,choose reasonable application of antibiotics,especially the ESBLs strains.We should strengthen the resistance testing,to provide reference for clinical rational drug use.

Behavioral Medicine ; Humans ; Public Health ; methods

The diffuse large B cell lymphoma (DLBCL) is the most common adult non-hodgkin lymphoma.At present,rituximab in combination with CHOP program has significantly improved the prognosis of DLBCL,and about 50％ of the DLBCL can be cured.However,due to the heterogeneity of the tumor,the refractory and relapsed DLBCL is still lack of effective treatment methods.With the application of gene expression profile (GEP) and the thorough research of the activation of lymphoma cells signal way,a lot of potential therapeutic targets have been found.

Objective To investigate the expression of pro-apoptotic factor (Smac) and Survivin in gastric ulcer tissue.Methods Selected the 80 cases of gastric ulcer patients as the research object in the first people′s hospital of neijiang,in which no precancerous lesions of 40 cases of gastric ulcer (N group),40 cases of precancerous lesions of gastric ulcer(Y group),two groups of patients were Smac mRNA and Survivin mRNA were detected by using PCR method,immunohistochemical SP method of Smac and Survivin in specimens of table detect.Gastric ulcer patients were treated by triple therapy,and the apoptosis index was detected by TUNEL.Results Smac in N group(++) and (+++) in the expression accounted for 82.5％ was higher than that in Y group accounted for 47.5％,Survivin in group N (++) and (+++) in the expression accounted for 15.0％ was significantly lower than Y group accounted for 35.0％;And Smac mRNA in the N group relative expression the amount was significantly higher than that of Y group,while the expression of Survivin mRNA in the N group were significantly lower than Y group;N group the apoptosis index of the triple therapy after treatment than before treatment significantly decreased,the difference was statistically significant(P<0.05).Conclusion The clinical application of Smac and Survivin can be used as an auxiliary diagnostic index for the diagnosis of precancerous lesions in patients with gastric ulcer.Patients with gastric ulcer without precancerous lesion treated by triple therapy which can effectively control the apoptosis index of patients,improve the survival rate of patients.