Objective To discuss clinical nursing points of patients undergoing left-right subclavian artery bypass grafting. Methods Effective nursing measures for 11 patients undergoing left-right subcla-vian artery bypass grafting preoperation,during the operation and postoporation were summarized retrospec-tively. Results All 11 patients were cured after systematic nursing postoperation. Conclusions Sys-tematic perioperative nursing plays a pivotal role in ensuring surgical effect and improving rehabilitation.

Objective To study antidepressant effects of Epimedium brevicornum extracts.Methods(Behavioral) despair models of male mice,tails suspension test(TST),and forced swimming test(FST) were used to evaluate the effects of E.brevicornum extracts on behavioral,monoamine oxidas(MAOA) and monoamine oxidase B(MAO-B) activities in brain and liver tissue,and MDA level in liver tissue of mouse.Reserpine antagonistic model was also used to investigate possible antidepressant mechanisms of E.brevicornum extracts.Results The extracts of E.brevicornum(25,50, 100,and 200 mg/kg) significantly reduced the duration of murine immobility in TST and FST,inhibited MAO-A and MAO-B activities in brain and liver of mice,and reversed the elevated liver MDA level in mice in TST.There was no significant amelioration in the decreases of body temperature in mice of Reserpine antagonistic model.Conclusion(E.brevicornum) extracts possesses the definite antidepressant properties.

AIM:The different effect of bivalent immunoglobulin Yolk(IgY) was evaluated against snake venom between intragastric administration and intraperitoneal injection in mice with cobra or viper envenomation.METHODS:The venom of naja and viper was injected alternately into the leghorn hen.Bivalent anti-snake venom IgY was extracted by water dilution.The concentration of bivalent IgY in plasma was observed in indirect ELISA assay after bivalent anti-snake venom IgY taken orally.The gastric emptying function test was used for determining optimization time after gastric administration of IgY.The protective effect of bivalent anti-snake venom IgY was compared between intragastric administration and intraperitoneal injection in mice with cobra or viper envenomation.RESULTS:Bivalent anti-snake venom IgY was extracted from eggs laid in 28-42 d after the first immunization.The titers of Bivalent IgY against cobra and viper venom were 1:12 800 and 1:6 400.At the time of 2.5-3.5 h after bivalent anti-snake venom IgY was taken orally in three concentrations(75 mg,150 mg,300 mg?0.5 mL-1?20 g-1 BW),the gastric evacuation rate of mice was above 68.9%,with the plasma concentration of bivalent IgY in peak.The survival time of mice envenomation with snake venom was extremely prolonged(P

Objective To explore the application effects of simulation diagnosis teaching in practical teaching of obstetrics and gynecology.Methods Students were divided into simulation teaching group(n=46) and traditional teaching group(n=46).Survey was conducted among simulation teaching group and traditional teaching group concerning clinical teaching of obstetrics and gynecology.Teaching effects were evaluated by questionnaire and examination.SPSS 13.0 statistics software was used to do the analysis.Enumeration data was analyzed by chi-square test while measurement data by t test.P＜0.05 stands for statistically significant difference.Results There were significant differences in improving students' clinical thinking and practical ability between two groups(P＜0.05).Satisfaction degree of simulation teaching group was significantly higher than that of traditional teaching group.Examination results of simulation teaching group was significantly higher than that of traditional teaching group(theoretical exam:P=0.001; operational exam:P=0.000).Conclusions Simulation diagnosis teaching can provide students more opportunities to practice and improve students' clinical thinking ability and clinical practice ability.

Objective To observe the effect of humanistic nursing care in rehabilitation ward.Methods 440 inpatients in rehabilitation ward were randomly divided into the observation group(228 cases,treated with humanistic nursing)and control group(212 cases,treated with traditional nursing).40 cases in each group received the investigation of patients' satisfaction to the service.Results The satisfactory rate of the patients in the control group to the attitude of the service,the nursing skill and health education missionary were 72.5%,75% and 67.5% respectively,but that of the patients in the observation group were all 100%,there was a significant difference between two groups(P<0.01).Conclusion Humanistic nursing care in rehabilitation ward can improve nursing qualification and increase patients' satisfactory rate.

Humans ; Tachycardia, Ventricular ; genetics

Objective To observe the clinical efficacy of the combination of western medicine with acupuncture for treatment of polycystic ovary syndrome (PCOS) of infertility.Methods Totally 120 PCOS patients were randomly divided into western medicine group and acupuncture group, 60 cases in each group. All patients took Diane-35, and with whom resistant to insulin were added with Metformin. At the meantime patients increased the amount of exercise with adjustment and control of everyday diet. After three periods, the western medicine group used the method of Clomifene Citrate with Chorionic Gonadotropin to help ovulation induction. The acupuncture group used the method of acupoint selection including invigorating spleen and kidney with adjusting qi and blood to promote ovulation. The level of FSH, LH, FINS, E2 and T of the two groups were observed before and after treatment.Results After treatment, the levels of T, FINS, LH and BMI in the two groups decreased, with statistical significance (P<0.05). The levels of T and BMI in the acupuncture group were lower than the western medicine group after the treatment, with statistical significance (P<0.05). The total efficiency, gestation number, number of high-quality follicle, and basal body temperature of acupuncture group were all higher than the western medicine group, with statistical significance (P<0.05).Conclusion The combination of western medicine with acupuncture for treatment of PCOS of infertility has confirmed efficacy.

Objective To compare the biochemical and clinical features of antimitochondrial antibody (AMA) positive and negative primary biliary cirrhosis (PBC),and to compare the sensitivity of both the detection of M2 antibody using enzyme linked immunosobent assay (ELISA) with antigen expressed from human sources,and the detection of AMA using conventional indrect immunofluorecence for diagnosing PBC.Methods AMA and M2 antibodies were tested in 63 patients with diagnosed PBC.Results Serum AMA positive rate in PBC patients was 81%(51/63) compared with 100%(63/63) of M2 positive rate.Antinuclear and anti smooth muscle antibodies(ANA and ASMA) were more frequently positive in the AMA negative patients (83% vs 35%,and 42% vs 4%, P

Objectives:To investigate the effect of glutamine on the shape of residual intestine and colon in rats with short bowel syndrome.　Methods:23 male Sprague-Dawley rats,underwent a 80% small bowel resection,were randomly divided into three groups:food group(n=8) rats,fed rat chow and water libitum after operation;TPN group(n=8),infused with Gln-supplied TPN;and normal control group.On seventh day after operation,rats were weighted and remaining jejunum、remaining ileum and colon were harvested for histological observation(light microscopy and electron microscopy).　Results:There was significant difference in rat average weight between food group and Gln group after operation.Jejunal mucosal villus height(VH) and mucosal thickness(MT) and ileal mucosal VH in food group were significantly increased than those in control group.Jejunal mucosal VH and MT in control group were significantly higher than in TPN group.Ileal mucosal crypt depth(CD) and MT in control group were also significantly higher than in TPN group.Jejunal and ileal mucosal VH、CD and MT in Gln group were significantly higher than in TPN group.Colonic MT in food group was significantly higher than in control group.Colonic MT in Gln group was significantly bigger than in TPN group.　Conclusions:After 80% intestinal resection,the remaining intestine can develop the adaptation,but the adaptation is incomplete.TPN therapy can maintain body weight,but only TPN can not result in the adaption.Gln-supplied TPN can stop the remaining itestinal mucosal atrophy,and promote the remaining intestinal adaptation and colonic mucosal hypertrophy.

AIM: To study the effect of caffeine on the large conductance calcium activated potassium (K_(Ca)) channels by patch-clamp technique on smooth muscle cells enzymatically isolated from the porcine coronary artery (PCASMC),and to investigate the effect of ryanodine on K_(Ca) being activated by caffeine.METHODS: Using the single channel patch-clamp technique,single PCASMC was isolated by collagenase,the activity of single K_(Ca) channel was recorded in porcine coronary artery smooth muscle cells.RESULTS: Caffeine (0.1-10 mmol/L) enhanced the open probability (Po) of K_(Ca) channels in a dose-dependent manner in the intracellular side of inside-out patches and its effect was almost completely abolished by washout. Caffeine decreased the mean close time markedly,but had no effect on the amplitude of K_(Ca) channels. However,ryanodine (10-40 μmol/L) decreased Po of K_(Ca) channels activated by caffeine in a dose-dependent manner in cell-attached patches. The mean open time also decreased.CONCLUSION: Caffeine directly activates K_(Ca) channels of porcine coronary artery smooth muscle cells in inside-out patches,the activity of single K_(Ca) channel is inhibited by ryanodine indirectly in cell-attached patches.