To study the dynamic changes and the significance of oxygen partial pressure in tissues in rats with traumatic shock, a traumatic shock model in Sprague Dawley rats were reproduced by limbs wounding, and oxygen partial pressure in tissues, including skeletal muscles,liver and small intestine, were assayed before trauma and after shock based on oxygen dependent quenching. Meanwhile, hemodynamic parameters of the rats were monitored. The results showed that oxygen partial pressure in tissues decreased significantly after traumatic shock( P

In order to investigate the dynamic changes in nitric oxide synthase (NOS) activity in organs during traumatic shock and to evaluate the effects of inhibitor of NOS and L Arginine (L Arg) on traumatic shock, rat model of traumatic shock was established, and the constitutive NOS and inducible NOS in the heart, small intestine, liver, lung , spleen were measured 0 5h, 3h, 5h after traumatic shock. Followed by intravenous injection with aminoguanidine (AG ), N G nitro L arginine methyl ester (L NAME), or L Arg, NOS were again measured, and the survival time and 24h survival rate were recorded. It was found that in normal rats cNOS was expressed in all organs, but only a weak iNOS expression in lung and liver. Half an hour after shock, cNOS was elevated in almost all organs in various degrees but there was no change in iNOS. 3h after shock, an increase in iNOS activity and a decrease in cNOS were observed. The iNOS was synthesized in large quantity 5h after shock. AG and L Arg markedly prolonged the survival time but L NAME did not in shock rats. AG inhibited the activity of iNOS but promoted the synthesis of cNOS, and both NOS were inhibited in L NAME group, but not in L Arg group. The results that iNOS was synthesized in large quantity only at later period of traumatic shock. To treat shock, the inhibitors of NOS and L Arg should be given according to the degree of shock, the dosage of drugs, and the time of administration

The aim of this study was to investigate the significance of low blood pressure (permissive hypotension) resuscitation in the treatment of uncontrolled traumatic shock. A traumatic shock animal model was replicated in Sprague Dawlay rats, the animals were randomly devided into non resuscitation group, traditional resuscitation group and low blood pressure resuscitation group. Prothrombin time(PT), activated pactial thromboplastin time(APTT) and oxygen partial pressure in tissues, including skeletal muscles, liver and small intestine, were determined before and after shock. Meanwhile, hemodynamic parameters of the rats and their survival time were monitored and recorded. The data showed that there were statistically significante difference in PT, APTT and amount of blood loss during shock between traditional resuscitation group and the other two groups. Low blood pressure (permissive hypotension) resuscitation could significantly improve oxygen partial pressure in tissues and prolong the mean survival time. The data suggested that low blood pressure resuscitation was a more rational strategy to improve the prognosis in rats with uncontrolled traumatic shock than traditional resuscitation strategy.

To study the role of endothelin(ET) in the pathogenesis of traumatic shock. Multiple injuries involving the leg bones, the soft tissue and hemorrhage in Sprague Daulay rat were produced by smashing its legs on both sides to induce traumatic shock. Dynamic changes in plasma ET, arterial blood gas analysis (ABGA) and oxygen partial pressure in tissues including skeletal muscles,liver and small intestine were detected at pre trauma and post shock periods. Meanwhile, hemodynamic parametars of the rats and their survival time were monitored and recorded. The data showed that plasma ET levels increased significantly after traumatic shock, reaching a peak of about 2 5 times of normal levels. There was statistically significant difference of ABGA values and tissues oxygen partial pressure between pre trauma and post shock periods. Metabolic acidosis appeared during traumatic shock. The changes in oxygen partial pressure in liver and small intestine were more significant than that of skeletal muscles. The data suggested that the significant increase in plasma ET contents after traumatic shock, might plays an important role in maintaining the blood pressure in the earty stage of shock, but might be an important cause of death in the late stage. The irreversible decrease in oxygen partial pressure in internal organs might be the key factor of refractoriness to resuscitation in an late stage of shock

Objective To study osteogenic courses and features of human osteoblasts cultured in vitro and seeded into alloplastic decalcified cancellous bone.Methods Human osteoblasts isolated from normal human perios- teum were cultured and amplified in vitro.Thy configuration and developing manners of osteoblasts were observed. Then osteoblasts were seeded into alloplastic decalcified cancellous bone.Thy composites were implanted and cultured in thy body of athymic mice.The specimens were obtained 8 weeks later.Osteogenic characteristics were observed by scanning electron microscope.Results Osteoblasts isolated from human periosteum developed well and proliferated rapidly in vitro.Island new bone formation could be observed histologically 8 weeks after the composites of os- teoblasts and alloplasric decalcified cancellous bone were implanted and cultured in the body of athymic mice.Conclu- sion Calcified bone can be formed by seeding osteoblasts into alloplastic decalcified cancellous bone,which may be a new kind of bone graft source to construct bone defects.

Based on the findings recently reported, cysteinyl leukotriene receptors, both CysLT (1) and CysLT(2) receptors, are involved in the ischemic and traumatic brain injury in vivo. CysLT(1)receptor regulates the increased permeability of blood-brain barrier and the related vasogenic brain edema, astrocyte proliferation, and inflammatory responses after brain ischemia; while CysLT(2)receptor regulates AQP4 expression and the related cytotoxic brain edema, and astrocyte injury. A new subtype of CysLT receptor, GPR17, is also involved in brain ischemic injury. The roles of CysLT receptors in brain injury or neuroprotection from the injury should be further understood. This understanding is necessary to accelerate the screening and development of the new drugs for the prevention and treatment of brain injury with the receptors as therapeutic targets.
Aquaporin 4 ; metabolism ; Brain Injuries ; metabolism ; Brain Ischemia ; metabolism ; Humans ; Receptors, G-Protein-Coupled ; metabolism ; Receptors, Leukotriene ; metabolism

OBJECTIVETo understand the status of occupational health of female migrant workers in different kinds of pharmaceutical industries in Gansu province and to provide the basis for improving occupational health condition.

METHODSOne thousand eight hundreds and one female workers from 16 enterprises were selected by cluster sampling in Gansu province and investigated by interviewing and questionnaires.

RESULTSThere were statistical significances of education level, status of residency registrations, employment relationship and occupational hazards among female workers in three types of enterprises (P < 0.05 or P < 0.01). The morbidities of skin disease in female workers for three kinds of enterprises were 4.46%, 2.53% and 3.70%, respectively. The morbidities of reproductive system disease in female workers for three kinds of enterprises were 48.57%, 36.70% and 36.11%, respectively.

CONCLUSIONThe levels of education and working conditions of female workers in the traditional Chinese medicine, western medicine plants are low. There are more severe occupational hazards in female workers of the traditional Chinese medicine plants.

Adolescent ; Adult ; Drug Industry ; Female ; Health Status ; Humans ; Middle Aged ; Occupational Exposure ; Occupational Health ; Surveys and Questionnaires ; Transients and Migrants ; Young Adult