Objective To explore the effects of parents'participatory functional training on the prognosis of newborn babies with hypoglycemic brain injury.Methods Functional training was given to 32 parents of newborn babies with hypoglycemic brain injury and then carried out on their babies for three months.The clinical symptoms and clinical efficacy of the babies and parents'satisfaction with nursing care were then observed.Results The clinical symptoms of the babies were improved significantly after training, and the parents had significantly higher satisfaction with nursing care as compared to pretraining (p<0.01).After training, 22 babies showed significant clinical efficacy (68.75%) , 8 babies had the targeted effect (25.00%);and the total effect rate was 93.75%.Conclusion Parents'participatory functional training helps to improve the prognosis of newborn babies with hypoglycemic brain injury and reduce the incidence of neurological sequelae.

6 months (n=12), control group, without AF (n=8). Atrial muscle samples of the left and right atrial appendage were cut off during heart valve replacement. Expressions of Cx40 and Cx43 were detected by immunoblotting and immunohistologic analyses. Results ①No obvious change of Cx43 and Cx40 in the left and right atrium of each patient in each group was measured. ②Compared with that in the control group, the level of Cx40 was decreased signficantly in GroupⅠ (P

6 months; Control group (n=8) without AF. Atrial muscle samples of the left and right atrial appendage were cut off during heart valve replacement. Morphological changes were observed by light microscopy and electron microscopy. Results ① The incidence rates of Aschoff body were 37.5% (3/8), 46% (5/11) , and 50% (6/12) in Group Ⅰ, Group Ⅱ, and the control group. There was no difference in Aschoff body between the left and right atrial myocytes. ② There was no difference in myocytic degeneration, interstitial hyperplasia, and lymphocytes infiltration between the left and right atrial myocytes in each group. ③ Myolysis, accumulation of glycogen, and fibrosis were detected by electron microscopy in the 3 groups. Changes of myolysis and fibrosis were more obvious in patients with AF than patients without AF. Conclusion There are similar pathological changes in the left and right atria in rheumatic heart disease patients with or without AF. Fibrosis of atrial myocytes may be involved in the occurrence and maintenance of AF.

Objective: To explore early diagnostic value of combined detection of ischemia-modified albumin and other biochemical markers for acute coronary syndrome (ACS). Methods: A total of 156 patients, who received treatment within 4h because of chest pain onset, were divided into ACS group (n=112) and non-ischemic chest pain group (NICP group, n=44) according to coronary angiography (CAG). Blood sample was taken within 4h and during 4~8h after chest pain to measure cardiac biochemical markers, including ischemia-modified albumin (IMA), LDH, CK, CK-MB and cardiac troponin I (cTnI), comprehensive analysis was performed by detected results of above biochemical markers and CAG. Results: For diagnosis of ACS, within 4h after onset IMA possessed highest sensitivity (87.50%) and highest accuracy (80.77%), cTnI possessed highest specificity (95.45%); In 4～8h IMA still possessed highest sensitivity (91.07%), cTnI still possessed highest specificity（97.73%）, CK（85.90%），and IMA（85.25%）possessed highest accuracy （both CK and IMA had no significant difference）; According to comprehensive analysis, regardless of within 4h or during 4~8h after chest pain, IMA possessed highest sensitivity and highest accuracy, cTnI possessed highest specificity. Within 4h after chest pain, detection of IMA combined above-mentioned other four indexes increased sensitivity (89.28%), specificity （95.45%） and accuracy （91.02%）to highest level. Conclusion: For diagnosis of ACS, IMA possesses highest sensitivity and highest accuracy, cTnI possesses highest specificity; IMA combined above-mentioned other four indexes may increase sensitivity, specificity and accuracy to highest level.

Objective:To investigate the expression of miR-126-3p in thyroid cancer and the biological function.Methods:The expression of miR-126-3p in thyroid carcinoma,adjacent tissues and three types of thyroid cancer cells(TPC-1,FTC-133,8505C) were detected by RT-PCR;thyroid cancer cells were divided into analogue group(mimic) and control group(NC),which were respectively with the transfection of miR-126-3p mimic and negative control plasmid.The proliferation and apoptosis in two groups were respectively detected by Brdu-ELISA and flow cytometry.The migration and invasion were detected by Transwell Chambers method.Results:The expression of miR-126-3p in thyroid carcinoma was significantly lower than adjacent tissues(0.384±0.028 vs 0.981±0.039,t=10.291,P<0.05);the expression of miR-126-3p in TPC-1 was the lowest among three types of thyroid cancer cells.Compared with NC group,the proliferation of TPC-1 in mimic group was significantly inhibited,the same with migration(26.68±4.48 vs 82.21±3.65,t=17.789,P<0.05)and invasion(12.28±1.03 vs 34.43±2.10,t=8.103,P<0.05),which the apoptosis was significantly increased[(15.32±3.20)% vs (8.12±1.17)%,t=4.623,P<0.05].Conclusion:The miR-126-3p expression is reduced in thyroid cancer tissue,overexpression of miR-126-3p significantly suppresses the proliferation,migration and invasion of thyroid cancer cells,and promotes the apoptosis,miR-126-3p can play an important biological function as a cancer suppressor gene in thyroid cancer.

Objective To study the effects of treating limbs pain of patients with depression after cerebral apoplexy with self-designed hemiparalysis-washing formula.Methods 80 patients with limbs pain after cerebral apoplexy in our hospital from May 2013 to November 2013 were recruited into a control group and a treatment group randomly,40 patients in each group.The control group was treated with 30mg edamvone and 100ml 0.9％ sodium chloride or 0.5％ glucose injection,Ⅳ in 30min,and twice daily.Meanwhile,regular functional rehabilitation training was adopted for the patients.The treatment group was additional treated with self-designed hemiparalysis-washing formula on the basis of the control group.VAS,HAMD-24,MESSS,STREAM and therapeutic effects were evaluated in both groups after one month treatment.Results ① clinical therapeutic effects comparison:the total effective rate was 90％ and 70％ in the treatment group and the control group respectively,with statistical difference between the two groups (t=3.58,P＜ 0.01).② VAS,HAMD-24,MESSS,STREAM comparison:VAS,HAMD-24,MESSS,STREAM score was (2.28 ± 0.54)、(11.34 ± 3.02)、(13.49±4.27)、(44.76±20.71)in the treatment group respectively,and(2.90 ± 0.75) 、(14.24 ± 3.35)、(17.55 ±4.91) 、(34.56 ±15.56) in the control group respectively.Both groups showed obviously improvement than the before treatment,and the effects were better in the treatment group than the control group (all P＜0.05).Conclusion Self-designed hemiparalysis-washing formula improve limbs pain in patients with cerebral apoplexy and relieve their depression.

Objective To evaluate the feasibility and safety of the modified panceaticojejunostomy called Kissing panceaticojejunostomy by animal experiment,and to explore the pathological process of primary healing between the pancreatic duct and the jejunal mucous.Methods The tolerance pressure at the anastomotic intestine was measured during surgery to ensure close contact suture of Kissing panceaticojejunostomy.Exploratory laparotomy,pancreatic ductography and pathology was performed at 2,4 weeks after panceaticojejunostomy.Results Kissing panceaticojejunostomy was performed successfully on 6 Beagle dogs.Tolerance pressure in all dogs was 90 cm H2O and above.No bleeding、fistula or contrast extravasation was found,and healing anastomosis was identified by laparotomy and pancreatic ductography.Cicatricial healing was found between pancreatic duct and jejunal mucosa 4 weeks after anastomosis.Conclusions Kissing panceaticojejunostomy is easy to perform,ensuring close-up cicatricial healing without hydrops or abscess between pancreatic duct and jejunal mucosa.

Objective To study the effect of ischemia reperfusion(IR) on electrophysiological function of sinoatrial node(SAN) in rabbits. Methods Ninety healthy adult rabbits were divided randomly into control group, ischemia groups (I) 10(I10 min), 30(I30 min), 60(I60 min) and 120 min(I120 min) and IR groups (10, 30, 60 and 120 min ischemia followed by 4 h reperfusion respectively)(I10 minR4 h, I30 minR4 h, I60 minR4 h and I120 minR4 h). There were 10 rabbits in the control group and each subgroup. IR injury model of SAN was established by occluding and loosening the start section of right coronary artery(RCA). The changes of AA interval and arrhythmia were recorded by electrocardiography and cardiac chamber electrography synchronously. Results ① There was no significant difference in AA interval between different time points in the control group. ② Sinus arrest(SA), sinus bradycardia with arrhythmia(SB), or atrial tachyarrhythmia(AT) were found in 51 rabbits (63.75%) out of 80 rabbits in I and IR groups in ischemic period. The incidence rate of arrhythmia increased in a time dependent manner. The AA intervals also increased by at least 40 ms in 54 rabbits (67.5%). ③ Sinus or atrial arrhythmia during ischemic period was found in 26 out of the 40 rabbits in IR group, but 15 returned normal after reperfusion. ④ Increased AA intervals were found in 27 out of the 40 rabbits in IR group during ischemic period. Most of them recovered to pre occlusion level within 10 min after reperfusion, but the AA intervals prolonged again in I60 minR4 h and I120 minR4 h groups as the reperfusion elongated. Conclusion ① These findings suggest that about 2/3 of the rabbit sinus node arteries may stem from right coronary artery. ② Electrophysiological changes due to ischemia of SAN resemble the electrocardiogram of sick sinoatrial node syndrome. ③ Reperfusion arrhythmia can be induced by reperfusion after a long time of ischemia.

Objective:To investigate if in vitro chemotherapy can induce the EMT progress in gastric cancer (GC) cells. Method:The GC cell line, SGC7901, was treated using 5-Fu at a concentration of 30 μg/mL. The residual cells after four cycles of 5-Fu therapy were named as SGC7901/Fu. The morphological changes and malignant biological features, including the invasiveness and clone formation ability and the characteristics of cancer stem cell and biomarkers of EMT between SGC7901 and SGC7901/Fu, were compared. Results:The SGC7901/Fu cells displayed a mesenchymal appearance, decreased the expression of epithelial markers, and increased the expression of mesenchymal markers. The 50% inhibitory concentrations in the SGC7901/Fu and SGC7901 cells were (43.8 ± 7.2) and (64.6 ± 5.5)μg/mL, respectively. The number of cells that migrated through the basement-membrane of the Transwell chamber was 51.4 ± 8.7 and 93.2 ± 9.5, respectively. The rate of clone formation was 5.2%± 1.0%and 13.2%± 2.2%, respectively. The portions of the CD44+/CD24-cells were 4.13%±0.81%and 7.97%±0.50%, respectively. All differences were statistically significant (P<0.05). Conclusion:The residual GC cells underwent EMT progress after 5-Fu treatment, with increased chemoresistance and ability of invasiveness and acquired the property of cancer stem cells.

Objective　To study the effect of acute serious smoke inhalation injury to unilateral lung on contralateral lung and the therapeutic efficacy of massive bronchoscopic lavage with saline to the primary injured bronchoalveolus at early stage after smoke inhalation on mitigating the secondary damage of the other lung and so as to confirm the therapeutic validity of lung lavage to smoke inhalation injury. Methods　Fifteen mongrel dogs were insufflated with sawdust smoke into left lung and then randomized into 2 groups. The dogs in group A were maintained intravenous glucose saline transfusion and breathed air freely for 24 h after injury. Those in group B received a massive bronchoscopic lavage with 250 ml of saline to injured lung at 1 hour after injury and then were administrated similarly to those in group A. Gas exchange function of bilateral lungs in the process were observed and pathologic and phyiopathologic changes of the lung specimen were examined after the process. Results　While the total lavaging volume was kept to 250 ml, the liquid volume remained in left lung after each operating was from 90 ml to 140 ml. All animals had a significant decline in pulmonary function after smoke inhalation injury. PaO2 in group B decreased significantly soon after the lavage and then increased gradually to exceeding that in group A. The left lung showed serious edema similarly in two groups but in right lung the water content was less, dynamic and static compliance was more in group B than in group A. Conclusion　Bronchoalveolar massive lavage at early post-injury stage to injured lung after unilateral lung smoke inhalation injury can mitigate secondary damage of the non-injured lung. Lung lavage can decrease the intensity of secondary systemic inflammatory reaction and show a therapeutic validity to smoke inhalation injury.