The position,length,size,and the site and pattern of ramification of the innominate arteries and common carotid arteries were studied in 76 specimens(♂44,♀32)of one- year-old to three-year-old Chinese infants. 1.The position relation between the innominate artery and trachea may be divided into three patterns:type Ⅰ:2 infants(2.63?1.8%),type Ⅱ:33 infants(43.42? 5.72%),type Ⅲ:41 infants(53.95?5.71%). 2.In 76 specimens the ramification region of innominate arteries of the Chinese infants were studied,among which in 57 specimens(75.0?4.96%)the site of ramifi- cation of innominate arteries is situated above the upper border of the sternoclavicular joint,and in 19 specimens(25.0?4.96%)behind the sternoclavicular joint.The region of ramification is higher in position in the infant than in the adult. 3.The average length of innominate arteries of all 76 specimens of 3 age groups: one,two,and three years old,is 20.7?0.42mm,19.2?0.56mm and 21.8?0.94mm respectively.The average circumference is 19.8?0.64mm,20.3?0.21mm and 21.0? 0.51mm respectively. 4.In 63 cases(82.9?4.32%)the innominate artery arises from the aortic arch by itself,in the other 13(17.1?4.32%)it arises from a common trunk,which is formed by the union of the innominate artery with the left common carotid artery.Among the 76 specimens,71 cases did not give off branches on its way,but in 4 specimens(5.26? 2.56%)the thyroidea ima is branched off directly from the innominate artery on its way,while in 1 specimen(1.32?1.31%)it arises from the common trunk. 5.The average site of ramification of the common carotid artery of the 76 Chinese infants is situated at the level of npper border of the intervertebral disc between the third and the fourth cervical vertebrae,but it is different on both sides:37 cases(48.7? 5.73%)with a higher level on the left,14 cases(18.4?4.44%)with.a higher level on the right side,25 cases(32.9?5.38%)with both sides on the same level. 6.The ramification angle of the common carotid arteries of the 76 infants fall into three patterns:60 sides(39.5?3.96%)belong to the acute angle pattern,40 sides(26.3? 3.57%)belong to the arcform pattern and 52 sides(34.2?84%)belong to the in- termediate pattern.The acute angle pattern is more common than the arcform pattern in the infants. 7.The average length of common carotid arteries of all 76 specimens of the three age-groups in one-、two-、three-year-old Chinese infants was 42.9?1.55mm,45.7?0.57 mm and 47.8?1.50mm on the right side and 59.0?1.10 mm,56.7?2.99mm and 64.9?1.62mm on the left side respectively,but the average circumference was 14.8 ?0.77mm,16.3?0.14mm and 16.6?0.19mm on the right side and 15.6?0.61 mm, 16.6?0.13mm and 17.3?0.40mm on the left side respectively.

The aortic arch and its ramifications were studied in 76 chinese infant specimens (♂44,♀52)We examined its position,length and circumference and the types of its ramifications. 1.In average,the superior border of infant's aortic arch is situated at the level of the intervertebral disc between the second and third thoracic vertebrae,and the inferior border is situated at the level of the upper one-third of the fourth thoracic vertebra. 2.The medial point between the superior and inferior borders of the aortic arch,in average,is situated at the level of lower one third of the third thoracic vertebra.In the 3 age-groups,1,2 and 3-year old infants,in 76 specimens,we found that the levels of the medial point of aortic arches,corresponding to 6.05,6.10and 6.19 in the 7 th region of the vertebral column respectively,Comparison of this figures with those of the adult revealed that its height in the infant is higher than in the adult. 3.The average lengths of aortic arch of the 3 age-groups of the Chinese infants were 20.6?1.31mm,19.3?0.59mm and 20.8?0.43mm respectively. 4.The circumferences of the aortic arch of the 3 age-groups of the Chinese infants before the aortic arch divided int?the innominate artery,in average,were 35.8?0.86 mm,36.4?1.04 mm and 38.6?0.83 mm respectively,and after dividing into the left subclavian artery,in average,they were 26.1?1.01mm,26.9?1.25 mm and 29.7?0.79 mm respectively. 5.Seven types can be distinguished in the patterns of the ramifications of the aortic arch.One type was not included in the twenty types of the ramification that have been suggested by Chang Wei-lung according to McDdonald and Anson's classification.This new type increases the number of the types of the human aortic arch to twenty one.The seven types viz: Type A:52.infants,68.42?5.33%.Type B:11 infants,14.47?4.03%.Type G: 4 infants,5.26?2.56%.Type D:1 infant,1.32?1.31%.Type F:4 infants,5.26? 2.56%.(One of the four cases is named thyro-thymus trunk).Type BF:1 infant,1.32 ?1.31%.(This type was composed of a common trunk,which consisted of the inno- minate and left common carotid,the left subclavian and the thyroidea ima arising from the common trunk.According to De Garis's classification,we combine type B with type F into type BF).Type J:3 infants,3.95?2.23%.(This type was more common in infants than in adults). 6.By comparing the types of ramification of aortic arch in 76 specimens with those of the adult,it reveals that the types of the ramifications of aortic arches vary with age.

Objective To investigate the diagnostic value of procalcitonin (PCT) ,C‐reactive protein(CRP) and white blood cell (WBC) count in children with respiratory tract infection .Methods A total of 358 children inpatients with respiratory tract infec‐tion in the pediatric department of our hospital from 2014 January to June 2015 were selected and divided into the bacterial infection group and non‐bacterial infection group according to the throat swabs and sputum culture results .The venous blood was collected before and after treatment for detecting PCT ,CRP and WBC count ;meanwhile 30 healthy children were selected as the control group .Results The PCT ,CRP and WBC levels in the bacterial infection group were significantly increased compared with the non‐bacterial infection group and control group(P<0 .01) ,while which in the non‐bacterial infection group were relatively close to those in the control group(P>0 .05);but the PCT ,CRP and WBC count levels after 1‐week treatment in the bacterial infection group were significantly decreased compared with before treatment (P<0 .01) ,while which in the non‐bacterial infection group had little change between before and after treatment (P>0 .05) .Conclusion The combined detection of PCT ,CRP and WBC count has an important application value in the differential diagnosis and medication guidance of the children′s respiratory tract infection .

Purpose Bronchial arterial embolization is the preferred hemostasis method for hemoptysis when medical treatment is invalid. This paper aims to discuss the safety and efficacy of inferior phrenic artery (IPA) embolization in the treatment of hemoptysis of tuberculosis when IPA is involved. Materials and Methods Twenty-eight patients who were confirmed that IPA got involved in the hemoptysis due to pulmonary tuberculosis by IPA angiography underwent embolization. CT scan was performed before the procedure and IPAs arteriography were performed during the interventional procedure. Once the blood supply was identified, catheterization and embolization was carried out with gelatin sponge particle, sodium polymannuronate microsphere or microcoil according to the patients' conditions. After the procedure, the IPA angiographic manifestations, clinical efficacy and complications were evaluated. Results Thirty-three IPAs were identified as getting involved in the blood supply in the 28 patients. Among those patients, 12 had left IPA involved, 11 had right IPA involved and the rest 5 had IPAs involved in both sides. The selective IPA angiography showed IPAs had enlargement, with numberous and disordered branches and hypervascularity. IPA-pulmonary artery shunt was found in 22 cases. None of the cases was found extravasation of contrast medium. The hemoptysis reoccurred in 4 patients on the second day, sixth day and 6 months after the first embolization, thus the procedures were performed three times in 1 patient and twice in the other 3 patients. Nine patients had fever as complication, 19 patients had chest pain and 5 suffered from mild dyspnea. These complications usually disappeared in 3-7 days after symptomatic treatment. Conclusion IPA embolization is technically feasible and safe, whose complications are likely to be few and self-restrictive.

Aim To explore the role of Rho-kinase in remote ischemic postcondi-tioning and its possible mechanism.Methods Thirty male Sprague-Dawley rats were divided into five groups(n=6): sham group(Sham), ischemia/reperfusion group(I/R), remote ischemic postconditioning group(RIPostC), I/R with Rho-kinase inhibitor fasudil group(I/R+Fas) and RIPostC with Rho-kinase activator lysophosphatidic acid group(RIPostC+LPA).Throughout the whole process of experiment, mean arterial pressure(MAP), heart rate(HR) and Ⅱ lead electrocardiogram were continuously monitored.At the end of the reperfusion, plasma creatine kinase(CK) and lactate dehydrogenase(LDH) were measured.Myocardial histopathologic changes were observed by hematoxylin and eosin(HE) staining.Infarct size was measured using 2,3,5-triphenyltetrazolium chloride(TTC) staining.The expressions of phospho-myosin light chain(p-MLC) were detected with Western blot analysis.Results Compared with Sham group, the MAP and HR of other groups decreased, while the amplitude of ST segment increased.Compared with I/R group, MAP and HR increased, the amplitude of ST segment decreased, plasma CK and LDH activity decreased, myocardial pathological morphology and infarct size were improved significantly, infiltration of inflammatory cells was reduced, and the expression of p-MLC decreased in RIPostC and I/R+Fas group.Compared with RIPostC group, RIPostC+LPA group attenuated the effects of RIPostC, and the recovery of the above indicators were inhibited.Conclusion Rho-kinase signaling pathway might mediate remote ischemia postconditioning against myocardial ischemia/reperfusion injury.

ObjectiveTo investigate the protective effect of ozone oxidative preconditioning (OzoneOP) on apoptosis induced by acute renal ischemia/reperfusion (I/R) injury in rats.Methods The right kidneys of rats in control group were excised.A rat I/R model was established in I/R group.In OzoneOP group,the renal OzoneOP was induced by rectal insufflation of 5.0～5.5 ml oxygen plus ozone (ozone 50 mg/L,1 mg· kg- 1 · d- 1,once every day).The parameters (blood urea nitrogen and creatine )of renal function were determined by auto-biochemical analyzer. The concentrations of malondialdehyde (MDA) and superoxide dismutase (SOD) were measured by using chromometry.Cytochrome c (CytC) was examined by using immunohistochemistry and Western blotting.The mRNA expression levels of TNF-α,IL-1β and IL-6 in the renal tissue were detected by using RT-PCR.ResultsCompared with the control group,the blood urea nitrogen,creatine,MDA and the release of CytC were increased significantly in I/R group.After OzoneOP,the levels of blood urea nitrogen,creatine,MDA,CytC and the mRNA expression levels of TNF-α,IL-1-β and IL-6were significantly decreased and SOD levels were significantly increased.ConclusionOzoneOP could relieve renal I/R injury of rats by improving antioxidation capability,cutting down cytokines contents and inhibiting the release of CytC from mitochondria.

Objective To establish a single-tube detecting system for the simultaneous identification of JAK2 V617F and JAK2 exon12 mutations.Methods Genomic DNA of cell line PC-3 was utilized as the wild type control,while genomic DNA of cell line HEL and plasmids with diverse JAK2 exon 12 mutations were used as the positive controls for JAK2 V617F and exon12 mutations.Multiplex PCR was performed to amplify the different amplicons combined with high-resolution melting (HRM) analysis,which established the multiplex detecting system for JAK2 V617F and exon12 mutations.Meanwhile 42 cases of polycythemia vera patients were collected to detect 2 kinds of JAK2 mutations by the above system and routine methods.Results The multiplex JAK2 mutations detecting system was successfully established by multiplex PCR combined with high-resolution melting curve analysis,which could simultaneously detect JAK2 V617F and JAK2 exon12 mutations.The analytical sensitivities of 2 mutations in this system were both up to 5％ and the precision (coefficient of variation) of intra-and inter-assay of the melting temperature (Tm) of 2 amplicons were separately less than 0.01％.37 cases were identified JAK2 V617F mutations from 42 polycythemia vera patients,while 2 JAK2 exon12 mutations cases were found from 5 JAK2 V617F negative patients.Compared with routine methods,the results matched the rate of 100％.Two cases of JAK2 exon 12 mutations were confirmed to the mutation types of H538K539delinsL and F537-I546dul10 + F547L by cloning and sequencing.Conclusions This method can simultaneously detect two kinds of JAK2 mutations in the peripheral blood and will contribute to the molecular diagnosis of myeloproliferative neoplasms,especially polycythemia vera.

ObjectiveTo establish a simple and sensitive method to detect MPL515 mutations in peripheral blood of ET and PMF patients,and investigate the frequencies of the MPL515 and JAK2V617F mutations in Chinese patients.MethodsTotallv 261 patients of ET and 25 PMF cases were collected from Huashan Hospital of Fudan University and DNA samples were isolated from peripheral blood of these cases.SYBR GreenⅠreal-time PCR was used to detect JAK2V617F mutation.Taqman probe was designed to be specific for the three types of mutations ( MPl515wt,MPLW515L and MPIW515K).Real-time PCR was used to detect MPL515 mutations.Tbe results were confirmed by sequencing after T-A cloning.Results Among 261 ET patients,119 cases (45.6％ ) were identified as JAK2V617F mutation carriers and 7 cases (2.7％ ) were detected to be MPl515 mutation carriers,including 5 cases with MPLW515L,1 case with MPLW515K and 1 ease with MPLW515L + K.Additionally 10 cases with JAK2V617F(40.0％ ) and 3 cases with MPL515 ( 12.0％ ) were screened out in 25 PMF patients,including 1 case with MPLW515L and 2 cases with MPLW515L + K.One ET patient was found to harbor concurrent JAK2V617F and MPL515 mutations.ConclusionJAK2V617F mutation is the major molecular marker of ET and PMF,meanwhile MPL515 mutation is important and useful complement.

Objective To evaluate the immediate and short term therapeutic efficacy of percutaneous transluminal septal myocardial ablation (PTSMA) in patients with hypertrophic obstructive cardiomyopathy (HOCM) and to investigate the prevention of the related complications. Methods PTSMA was conducted in 3 patients with HOCM refractory to medication. The immediate effect was evaluated by pressure monitor, but the follow up effect by observation of the clinical symptoms and ultrasonic cardiogram. Results The left ventricular outflow tract gradients triggered by ventricular premature beats decreased from 143 mmHg (ranging from 70 to 180 mmHg) to 53 mmHg (ranging from 30 to 80 mmHg). Complete atrioventricular block (Ⅲ?AVB) with atrioventricular node rhythm was found in 1 case, and complete right branch bundle block (CRBBB) in another one. Follow up of the 3 cases at 6 months after operation revealed remarkable improvement of the patients' cardiac function (NYHA), disappearance of angina pectoris, and obvious attenuation of ventricular septal hypertrophy and SAM (systolic anterior motion of mitral) phenomenon. Conclusion PTSMA is an effective method with satisfactory short term effect for the patients with HOCM refractory to medication.

Objective To observe the effects of Triptolide on the expression of toll-like receptor 4 (TLR4) in renal ischemia/reperfusion (I/R) injury in rats. Methods A renal I/R model was established. Rats were randomly separated into the following experimental groups. Group 1, shamoperated control (n = 15) : rats were subjected to surgical manipulation, without the induction of renal ischemia. Group 2, I/R (n = 18): rats were subjected to left renal ischemia for 45 min followed by reperfusion. Group 3, TRI + I/R (n = 18): Before the I/R procedure (as in group 2), rats were intraperitoneally injected with TRI (0.4 mg/kg), once every day, three times. Rats were killed at the 1st, 3rd, and 5th day after I/R injury. The parameters of renal function were determined by autobiochemical analyzer. The expression of TLR4 was detected by RT-PCR and Western blotting. Results As comparedwith the sham-operated control group, serum BUN and Cr levels were significantly increased in the rats undergoing I/R procedure at the 1st, 3rd, and 5th day (P＜0. 01). After the treatment with TRI, the levels of BUN and Cr and the expression of TLR4 in the renal tissues were significantly decreased (P＜0. 05). Conclusion TRI could relieve renal I/R injury in rats by inhibiting the TLR4 expression.