Objective To investigate the effect of pegylated interferon combined thymopeptideenteric-coated tablets on CXCR3, CCR6 and serum IL-2 and soluble ligand in patients with chronic hepatitis.Methods 125 patients with chronic hepatitis B patients from June 2015 to June 2016 in our hospital were selected and divided into observation group 62 cases and control group 63 cases.The observation group was treated with pegylated interferon combined thymopeptideenteric-coated tablets, and the control group was treated with thymopeptideenteric-coated tablets, the expression of T cells in patients with CXCR3 and CCR6 was detected by flow cytometry, the serum levels of interleukin-2 and soluble interleukin-2 receptorwere measured by enzyme-linked immunosorbent assay, and compared the treatment of two groups of patients.Results After treatment, the levels of IL-2 and sIL-2R in the observation groupwere (16.4 ±5.6), (9.4 ±2.1), significantly lower than that in the control group, while CXCR3 and CCR6 were (5.8 ±0.9), (5.54 ±1.2), significantly higher than the control group, the difference was statistically significant (P<0.05), the levels of HBsAg, HBeAg and HBV-DNA in the observation group were (1.5 ±0.4), (1.6 ±0.9), (1.6 ±0.3), significantly lower than those in the control group, the difference was statistically significant (P<0.05), the levels of ALT, AST, TBIL, IBIL in the observation groupwere (34.5 ±8.7), (31.4 ±6.9), (14.6 ± 6.9), (9.4 ±2.9), significantly lower than those in the control group, the difference was statistically significant (P<0.05).Conclusion Interferon combined thymopeptideenteric-coated tablets therapy for chronic hepatitis B is helpful to improve the level of chemokine receptor and inhibit the degree of inflammatory reaction.It can help to improve liver function and inhibit viral replication in patients with hepatitis B.It is better than the treatment of chronic hepatitis with thymosin alone.

Objective To investigate the clinical characteristics of children with dyspepsia diarrhea and the effect of Clostridium butyricum powder combined with changweikang granules on the treatment of the children with this diseases.MethodsFrom February 2013 to October 2015 in department of pediatrics, Tonglu County the First People's hospital, 76 cases with dyspepsia diarrhea as the research object, were randomly divided into the experimental group and the control group, 38 cases in each group.The two groups were given changweikang granules, at this basis, the observation group were given clostridium butyricum powder.3 days for a course of treatment, the two groups were treated for 2coureses continuously.ResultsBefore treatment, there were no significant differences in clinical symptom scores between the two groups.After treatment, the scores of loss of appetite, abdominal pain, diarrhea, abdominal distension and stool in the experimental group were lower than those in the control group, the difference was statistically significant (P<0.05);Before treatment, the serum motilin, gastrin and somatostatin levels in the two groups were not statistically significant.After treatment, the serum motilin and gastrin levels in the experimental group were lower than those in the control group, and the level of serum somatostatin was higher than that of the control group, the difference was statistically significant;After treatment, the total effective rate in the experimental group was 92.11% higher than that in the control group (73.68%), and the difference was statistically significant (P<0.05).ConclusionPediatric indigestion diarrhea often showed loss of appetite, abdominal pain, diarrhea, abdominal distension and stool.The effect is good which clostridium butyricum powder combined with changweikang granules on the treatment of infantile indigestion diarrhea, it is worthy of clinical application.

Objective To observe the outcome of Nimodipine and Salvia Miltiorrhiza Composite in treating newborn hypoxic ischemic encephalopathy(HIE ) . Methods 128 HIE patients were randomly divided into four groups;-Salviae Miltiorrhiza Compsite treated group (n=32) , Nimodipine treated group (n 30) , Nimodipine plus Salviae Miltiorrhiza Composite treated group ( n =35) , and control group ( n =31) , in which the symptoms and signs , the reactions following treatment were observed .Results The total effective rates of Salviae Miltiorrhize Composite treated group , Nimodipine treated group , Nimodipine plus Salviae Miltiorrhiza Composite treated group were 81.3% (26/32) , 80% (24/30) and 97.1% (34/35) respectively , and the markedly effective rates were 53.1% (17/32) , 53.3% (16/30) and 88.6% (31/35) respectively . The total effective rates and the markedly effective rates of control group were 51.6% (16/31) and 6.5% (2/31) respectively ,the treated groups were higher than that of control group ( P

The article tries to discuss the usage of "modthod of strengthening qi" in treating minimal residual leukemia(MRL). We believe that the mechanisms for MRI are qi weakness and pathogenic factors staying in yinfen. By using the method of strengthening qi, we hope to achieve the effects of regulating yin and yang, qi and blood, and physiological functions of visceras and meridians, and enhance body immunity. Thus, we can prevent and treat MRL and resist tumor indirectly.

Objective To discuss the changes of plasma TXA2 and PGI2 levels in the rabbit model of pulmonary hypertension (PHA)induced by monocrotaline (MCT)when transplanted with endothelial progenitor cells (EPCs). Methods The rabbit pulmonary hypertension model was constructed by MCT induction. Rabbit endothelial progenitor cells were induced,separated and identified in vitro.50 New Zealand white rabbits were randomly divided into 3 groups,normal control group and model group were given M199 culture medium by tail vein injection,EPCs treatment group were given EPCs marked with fluorescent though tail vein injection. The levels of plasma TXB2 and 6-keto-PGF1αwere detected and their ratio were calculated after three weeks. Results Compared with model group,the content of 6-keto-PGFlαin plasma in EPCs group was (130.67 ±17.54)u mol/L,which was increased sinnificantly. The content of TXB2 was (402.89 ±27.98 )u mol/L,which was decreased significantly.The ratio of TXB2 to 6-keto-PGF1 alpha between two groups was statistically significant(P<0.01 ). Conclusion EPCs transplantation can reduce the content of TXA2 and increase the content of PGI2 in the pulmonary hypertension rabbits induced by monocrotaline,then reverse the damage of pulmonary hypertension.

Objective:To observe the effect of calcitonin gene-related peptide (CGRP) on the expression of osteoprotegerin (OPG) and receptor activator of nuclear factor-kappaB ligand (RANKL) in osteoblast cells through an in vitro breast cancer cell and osteoblast cell co-culture system. Methods:The metastatic breast cancer MDA-MB-231 or MDA-MB-435 cells were co-cultured with osteoblast MG63 cells to establish an in vitro microenvironment of bone metastasis of breast cancer. After treated with CGRP(1?108 mol/L),OPG and RANKL mRNA and protein expressions in osteoblast MG63 cells were examined by RT-PCR and Western blotting. Results:Expression of RANKL in osteoblast MG63 cells was up-regulated at both mRNA and protein levels when osteoblast MG63 cells were co-cultured with breast cancer MDA-MB-231 or MDA-MB-435 cells,while those of OPG in osteoblast MG63 cells were both down-regulated (P

Objective To assess the efficiency and safety of alendronate for the prevention and treatment of glucocorticoid-induced osteoporosis (GIOP).Methods The electronic databases of PubMed,EMBASE,Cochrane Library,Web of Science,Chinese BioMedical Literature Database (CBM) and Wanfang Data were searched for all randomized controlled trials (RCT) of alendronate vs.placebo.Two reviewers independently selected trials for inclusion,assessed trial quality using Jadad's scale and extracted the data.RevMan 5.1 software was used for data synthesis and Meta-analysis.Results Seven studies with 1111 patients were included.Compared with placebo,alendronate significantly increased bone mineral density (BMD) at the lumbar spine[MD ＝3.35,95％CI (2.67-4.02),P =0.000] and the femoral neck[MD =1.90,95％ CI (0.89-2.92),P =0.000] after 12 months of therapy.After 24 months of therapy,alendronate significantly increascd BMD at the lumbar spine [MD =3.91,95％ CI (2.37-5.45),P =0.000],but not at the femoral neck [MD =1.91,95％ CI (-1.15-5.02),P =0.22].Compared with placebo,no significant reduction was found by the use of alendronate in the incidence of vertebral fractures [RR =1.00,95％ CI (0.49-2.07),P =0.99] or nonvertebral fractures[RR ＝ 1.02,95％ CI (0.49-2.14),P =0.95].No difference was shown with the adverse event between the two groups[RR =0.97,95％ CI (0.90-1.05),P =0.47].Conclusions Alendronate is effective for the prevention and treatment of glucocorticoid-induced bone loss at the lumbar spine and the femoral neck with relatively good safety profile.Yet,there is no significant difference between the two groups in reducing the incidence of vertebral fractures and non-vertebral fractures.Large-scale RCT designed to observe whether different lengths of alendronate therapy will influence the efficiency should be conducted in the future and to further explore whether it can reduce the incidence of fractures.

Crocetin, a naturally occurring carotenoid, possesses antioxidant and antiatherosclerotic properties, of which the underlying mechanism remains unclear. In the present study, we examined the effects of crocetin (0.1, 1, 10 μmol·L(-1)) on angiotensin II (Ang II, 0.1 μmol·L(-1)) induced expression of vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs) and monocyte-endothelial cell adhesion. The effects of crocetin on the activation of nuclear factor kappa B (NF-κB) and intracellular reactive oxygen species (ROS) were also observed. The results demonstrated that crocetin notably suppressed Ang II induced NF-κB activation (P<0.01) and VCAM-1 expression (P<0.05, P<0.01) in HUVECs, accompanied by a markedly reduced monocyte-endothelial cell adhesion (P<0.05, P<0.01). In addition, preincubation with crocetin resulted in a significant enhancement of cellular antioxidant capacity (P<0.05, P<0.01), while Ang II induced intracellular ROS decreased markedly (P<0.05, P<0.01). These results indicated that crocetin was capable of suppressing Ang II induced VCAM-1 expression and monocyte-endothelial cell adhesion by suppression of NF-κB activation, which might be derived from the enhancement of antioxidant capacity and subsequent reduction of intracellular ROS.

Objective To evaluate the clinical efficacy and complication rate by open or retroperitoneal laparoscopic radical nephrectomy for renal tumor with stages T1N0M0 or T2N0M0.Methods Between October 2003 and October 2006,90 patients with renal cell carcinoma,which were clinically localized stages T1N0M0 or T2NOM0 Based on the patients' options to undergo retroperitoneal laparoscopic radical nephrectomy (group A,49 patients)and open radical nephrectomy(group B,41 patients).The clinical efficacy were compared between group A and group B,retrospectively.Results In group A,the operations of 46 patients were successful,4 cases occurred major complications(8.7%)during the follow-up visit which lasted for (21.9±6.1)months.The operations in group B were all successful.9 cases occurred major complications (22.5%)during the follow-up visit which lasted for(24.9±7.8)months.All cases were renal malignant tumors with pathologically confirmed stages T1N0M0 or T2N0M0 and there were no renal pedical lymph node metastasis.The age,weight,body mass index(BMI),tumor size,operating time and the time of follow-up were no statistically significant differences between the two groups(P＞0.05),while the blood loss,amount of postoperative drainage,time to ambulation,recovery of intestinal function after operation,hospital stay,use of analgesic and transfusion blood or plasma in group A were significantly reduced than those in group B(P＜0.01). Conclusions As compared with open radical nephrectomy,retroperitoneal laparoscopic radical nephrectomy afords patients with renal cell carcinoma an impreved postoperative course with minimal invasion,less pain,quicker recovery and less complications,while providing equally effective cancer control for patients with T1N0M0 or T2N0M0 tumor.

Background and purpose:Bone metastases leads to the destruction of bones by changing the level of bone turnover markers.The purpose of this study was to assess the clinical application value of bone turnover markers in bone metastases for non-small cell lung cancer,which included the diagnosis and spread behavior of bone metastases.Methods:AKP,β-CTx,OST and BALP were measured in 76 NSCLC with bone metastases patients and 44 normal people.Results:The level ofAKP,β-CTx and BALP in patients with bone metastasis was significantly higher than in the subjects without bone metastases.There were significant correlations among the bone turnover markers.The levels of BALP and OST were significantly positively correlated with the extent of bone metastasis.Patients with high-levels of CTx and low-levels of BALP had a higher risk of pathologic fracture.Conclusion:In patients with bone metastases from NSCLC,bone turnover markers can help make diagnoses and evaluate severity of disease.It potentially has a wide range of uses in clinical practice.