Objective To investigate the protective effect of short-term moderate exercise on ischemic/reperfused myocardium and its correlation to the activation of protein kinase C(PKC).Methods Fourty Wistar rats were randomly divided into 4 groups(n=10 respectively): control group(CON group),exercise group(EXE group),exercise + PKC inhibitor group(E+C group) and PKC inhibitor group(CHE group).The occurrence of arrhythmia,the recovery of cardiac function,and infarct size were observed by using the Langendorff-ischemia/reperfusion model in isolated rat heart in vitro.Results Recovery rate of LVDP(on the 30th and 60th minute of reperfusion) and RPP(on the 20th,30th and 60 minute of reperfusion) of EXE group were higher than those of CON and E+C groups(P

Objective: Attempt to use generalized graded unfolding model to analyze the item of "Autonomy in Learning Rating Scale for College Students " adequacy of the scale designing and evidence probability of the usage of unfolding model in costructing tests. Methods: Based on randomly selected 600 graduates’ response of the scale, item analysis was carried on in 497 potent subjects. Results: Subjects whose trait continuum were between 2.5-4.0 and -3.5～-3.0 were short, and all the item locations were negative; all the item discriminations were above 0.5; the item information functions of 2, 6, 7, 19, 21, 25, 49, 50, 51 were below 0.4098; the fit plots of the item and test satisfied the requirements. Conclu- sion: Fit index of the whole scale is good; some items need to be revised or deleted; GGUM could be used in constructing attitude scales.

Objective To improve the efficienct of bone defect reparing,we produced a new shaped bone substitude.And we study the result from repairing rabbit radial segmental defect.Method Twenty mature rabbits were made segmental radial defect on both sides.15 received allogenic bone powder/FS/BMP in the left,and allogenic bone powder/FS in the right.5 samples received no implant in the left as control and allogenic bone powder/FS in the right.4,8,12 weeks after operation,result were assessed by gross,radiographic and histologcal examination.Result the study showed that allgonic bone powder/FS/BMP was much faster and better than allogenic bone powder/FS in repairing the defect(P<0.05),Conclusion It is suggest that allogenic bone power/fs/BMP is a kind of high bone induce ability ,shaped,and absorbable bone substitute.

Objective To compare the effect of two operative methods,median-rid-section and sub axillary median-rib-section.Methods 152 cases underwent by median-rib-section and among them 12 cases underwent by sub axillany median rib section.Results The median-rib-section procided a satisfact incision(the area of ineision was 14cm?30cm),without renobing the ribs,with the musculature.Complete,and less trauma.And the method of sub axillary median-rub-section had more intrathoracre esposure(the area of inclsion was 14cm?20cm),and the thoractomy could be done directly and satisfactory.Conclusion The median-rib section is a good method in thoraxoctomy which makes the ribs,made less trauma and provides satisfactory exposure,and after surgery,the chest is closed tighly.The sub axillary media rib section provides a wider application while remaining the good charactery of common sub axillary section.

Objective To study the feasibility of reconstructing trachea by implanting autogenous costal perichondrium tracheal prosthesis.Methods Ten dogs were used.Three pieces of costal perichondrium of every dog were wrapped on a silicon bar used as skeleton and implanted into sternohyoid muscle adjacent to trachea to establish blood supply.Four weeks later,silicon bar was removed and perichondrium ring served as tracheal prosthesis.Trachea resection and prosthesis replacement was performed.Viability of dogs,gross and microscopic pathological changes were studied to evaluate the effect of prosthesis replacement.Results The respiratory tracts of the 8 survival dogs were not obstructed.Their diet,activity and bark were not different from those of the normal dogs.With abundant blood supply,the prosthesis fused tightly with the surrounding tissue.Anastomotic orifice healed without cicatricial tissue and granulation tissue.The internal surfaces of prosthesis were smooth and glossy with white tunica mucosa covered.Under light microscope,internal surfaces of prosthesis were found covered by pseudostratified columnar epithelium with few cilia.Under tracheal mucous membrane,there were fibrous membrane,neonate chondrocytes and striated muscle cells stratum successively.Fibrous membrane was composed of collagen and fibroblasts with a few inflammatory cells scattered.A small number of neonate chondrocytes were found under fiber membrane.Plasma of these chondrocytes looked pale after staining,which indicated that these chondrocytes were neonate.Striated muscle cells connected with the neonate chondrocytes firmly. Neonate capillaries and micro blood vessels of various diameters were abundant in striated muscle stratum.Conclusion It is feasible to reconstruct dog trachea using prosthesis constructed with autogenous costal perichondrium.

Objective To explore the treatment effect of Zuogui pill (ZGP) on immune premature ovarian failure rats.Methods Female mice model of POF was established by multiple sites subcutaneous injection of zona pellucida 3,and treated with different dosage ZGP (low, middle and high),with prednisone and diaethylstilbestrol as positive control. We detecteed apoptosis rate of granulosa and oocyte of murine ovaries by tunel method and observd the change of sexual cycle and weight. Results Compared with the control group,the weight of model group rat in the third to eighth weeks was significant difference (P

Objective To investigate the clinical efficacy of bromhidrosis with botulinum toxin A by needle free injection.Methods A total of 40 patients with bromhidrosis were collected and divided into needle free group and needle group.A comparative study was carried out in those subjects.Patients in needle free group received needle free injection of botulinum toxin A by INJEX30;Needle group received traditional injection by 26 G needles.Results In needle free group,16 cases were cured,3 cases were effective,1 case was ineffective,no significant pain,hematomas or necrosis were found.In needle group,15 cases were cured,4 cases were effective,and 1 case was ineffective;localized hematoma occurred in 2 cases.And cases in group needle experienced pain more intensely than cases in needle free group.Conclusions The INJEX30 improves the security,comfort and therapy compliance of botulinum toxin A injection in the bromhidrosis patients,with similar efficacy with traditional injection.

Objective To investigate the clinical therapeutic effect of acupuncture on pulmonary infection after acute cerebral infarction.Methods Seventy patients with pulmonary infection after acute cerebral infarction were randomly allocated to treatment and control groups, 35 cases each. The control group received routine medication and the treatment group, acupuncture in addition. Pre-treatment and post-treatment National Institutes of Health Stroke Scale (NIHSS) scores and clinical pulmonary infection scores (CPIS) were compared between the two groups. The correlation between the NIHSS score and the CPIS score was observed.Results There were statistically significant pre-/post-treatment differences in the NIHSS score and the CPIS score in the two groups (P<0.05,P<0.01). There were statistically significant post-treatment differences in the NIHSS score and the CPIS score between the treatment and control groups (P<0.05). The correlation between the NIHSS score and the CPIS score was low in the treatment group after treatment (r=0.417,P<0.05).Conclusions Acupuncture plus medication is an effective way to treat pulmonary infection after acute cerebral infarction. It can improve the NIHSS score and the CPIS score in the patients.

Objective To evaluate the performance of trilobed flaps for reconstruction of nasal tip defects. Methods Totally, 23 patients with nasal tip defects after tumor resection were enrolled into this study. Skin defects varied from 1.5 cm × 1.5 cm to 2.5 cm × 2.5 cm in size. According to the location and size of skin defects, trilobed flaps were designed, and the angle between two lobes ranged from 45° to 50°. The size of the first lobe was approximately equal to that of the skin defect, while the size of the second and third lobes decreased successively. During flap design, the integrity of nasal subunits was sufficiently considered, and the incision lines were placed along the boundary lines of the nasal subunit as far as possible, so as to conceal the incision scar with natural skin folds. Results After the operation, skin flaps survived with primary healing of incisions in all the patients. After 6 - 24 months of follow up, the color, texture and thickness of flaps were similar to those of their surrounding skin, and incision scars were inconspicuous. No malformation occurred in the nasal ala, nostril, nasal columella or surrounding organs, and satisfactory nasal appearance and function were achieved. Conclusion Trilobed flaps can be used to effectively repair relatively large skin defects of the nasal tip with satisfactory nasal appearance and function.

Objective To investigate the incidence and risk factors for surgical site infection (SSI)in clean wound orthopedics surgery.Methods SSI among patients receiving clean wound orthopedic surgery from April 1 to Sep-tember 30 in 2014 were monitored,risk factors for SSI were analyzed.Results A total of 665 patients were moni-tored,14 cases(2.11 %)of SSI occurred.Univariate analysis showed that types of skin,skin preparation modes, and ASA score were risk factors for SSI in clean wound orthopedic surgery (all P <0.05 ).Multivariate logistic analysis revealed that conventional skin preparation,ASA score ≥ grade Ⅱ,and duration of operation ≥90 minutes were independent risk factors for SSI in patients receiving clean wound orthopedic surgery (OR [95%CI ],3.96 [1 .07-14.70],6.45[1 .97-21 .11],and 4.08[1 .35 -12.30]respectively).Conclusion The improved method of skin preparation,grade I ASA score,and shortening duration of operation can reduce the incidence of SSI in clean wound orthopedic surgery,on the basis of improving the autoimmunization of patients,strengthening prevention and control of healthcare-associated infection can reduce the incidence of SSI in clean wound orthopedic surgery.