A method for measurement of neutrophil function and opsonic activity by chemiluminescence (CL) was reported in this paper.The factors influencing the emission of CL were discussed. The experimental results suggested that CL depends on the concentration of luminol, the dilution of serum opsonin, the number of neutrophils and the environmental temperature. The optimum conditions defined in present study were: the concentration of luminol was 1?105M, the number of neutrophils 2?106/mml, the reactive temperature 37℃. According to the relationship between CL emission and the bactericidal activity of phagocytizing neutrophils, the measurement of CL may be used as a rapid, simple and usefull tool to determine the funetion of humoral-phagocyte axis.

Objective To analyze the effects of dopamine and norepinephrine on blood oxygen,heart rate and internal environment in patients with septic shock.Methods 80 patients with septic shock were collected.The patients were randomly divided into two groups.The control group was given dopamine treatment,the observation group was given dopamine and norepinephrine.Results The heart rate (HR)[(90.39±15.29)/min],ScVO2 index[(77.03±7.68)％] of the observation group were better than those of the control group (t=4.122 5,2.141 5,all P＜0.05).The urine volume[(94.05±22.39)mL/h],creatinine clearance rate[(53.12±14.45)mL/min] of the observation group were better than those of the control group (t=3.660 6,2.023 2,all P＜0.05).ConclusionDopamine combined with norepinephrine has significant effect on patients with septic shock,which can effectively improve the HR,ScVO2,urine volume,creatinine clearance rate and other indicators,thus to restore the patients'' internal environment.

Objective To explore the clinical effectiveness and safety of percutaneous vertebral augmentation with the Vessel-X bone void filling container system (Vesselplasty). Methods Three cases of fresh osteoporotic vertebral compression fracture were treated with Vesselplasty. After procedure, the pain relief, the fracture reduction, and the cement distribution in the vertebra were observed. Results All the 3 cases were treated with the unipediclar injection technique. The operative time was 45, 32 and 30 min, respectively. The hemorrhage volume was

Patients with ischemic stroke is often accompanied by inflammtory response. Studies have suggested that toll-like receptor 4 (TLR4) has induced the occurrence,development and of ischemic stroke and secondary brain injury. This article reviews the toil-like receptor 4 signal transduction pathway and its roles in ischemic stroke in order to provide certain basis for the development of TLR4-targeted medication.

Objective To investigate clinical significance and related factors of fluid-attenuated inversion recov?ery vascular hyperintensities (FVH) in transient ischemic attack (TIA) of carotid system. Method Data including general information and TIA risk factors was continuously collected from 142 patients with carotid system TIA from the depart?ment of neurology of Sheng jing Hospital affiliated China Medical University from January 2012 to February 2014.All pa?tients completed brain MRI including FLAIR and diffusion-weighted imaging (DWI)and MRA examinations within 72 hours after TIA. All patients were followed up for one month. Risk factors and FVH situations were analyzed based on clinical manifestations and DWI results. Result There were 87 male cases (61.27%)and FVH positive 57 cases (40.14%) among 142 cases with carotid system TIA (mean age 63.2±11.5). Logistic regression analysis revealed that the large intra?cranial carotid artery stenosis≥50%(OR=2.44,95%CI:1.09~5.49, P=0.03) and prior history of ischemic stroke (OR=3.88,95%CI:1.04~14.5, P=0.04) were independently associated with positive FVH. At one month followed-up, 40 cas?es (28.17%) of 142 patients progressed to acute cerebral infarction. Vulnerable plaque number in the contralateral carot?id artery (P=0.018), contralateral intracranial large vessel stenosis in MRA≥50%(P=0.007) and contralateral FVH oc?currence rate (P=0.001) were significantly higher in cerebral infarction group than in non-cerebral infarction group. Con?clusion FVH is common in carotid TIA patients, which is associated with intracranial carotid artery stenosis ischemic and previous history of ischemic stroke. Vulnerable plaque number of contralateral carotid artery, contralateral intracranial large vessel stenosis≥50%and the rate of occurrence of contralateral FVH may be associated with short-term progress leading TIA to acute infarction.

This paper explored factors influencing initiatives of clinical teachers in millitary medical university for training clinical undergraduates including work pressure after being transferring to civilian,work pressure,policy orientation,economic pressure,student factor and psychological factor,etc. Meanwhile,this paper explored the incentive mechanism of clinical teachers in millitary medical university for training clinical undergraduates including training and evaluating clinical teach-ing teachers, innovating evaluation criterion and creating sustainable teaching atmosphere (building examples and establishing various incentive systems). All measures taken above was mean to inspire the initiatives of clinical teaching teachers and to ensure the quality of clinical teaching.

At present, the formal bariatric surgery approved by the American Society for Metabolic and Bariatric Surgery (ASMBS) includes adjustable gastric banding (AGB), sleeve gastrectomy (Sleeve gastrectomy, SG). ), Roux-en-Y gastric bypass (Roux-en-Y gastric bypass, RYGB), biliopancreatic diversion with duodenal switch (BPD-DS), mini gastric bypass (Mini-gastric bypass, MGB), single anastomosis gastric bypass (OAGB) and partial endoscopic surgery. According to the Chinese Society for Metabolic and Bariatric Surgery (CSMBS), the Chinese Obesity and Metabolic Surgery Database (COMES Database), and the Chinese Obesity and Metabolic Surgery Database (COMES Database), the Chinese Society for Metabolic and Bariatric Surgery (CSMBS) Chinese Obesity and Metabolic Surgery Collaborative, COMES Collaborative), Shanghai Weight Loss and Diabetes Surgery Data Management System, Shandong Province Weight Loss and Metabolic Surgery Case Registration System, Jiangsu Province Weight Loss and Metabolic Surgery Data Registration System, Great North China Weight Loss and Metabolic Surgery Statistics from the clinical data database and the Greater China Weight Loss and Metabolic Surgery Database. The domestically developed and approved bariatric surgery methods include RYGB, SG, MGB, OAGB, BPD-DS, AGB, combined sleeve gastrectomy (SG Plus), Jejunoileal Bypass (Jejunoileal Bypass, JJB), single stoma duodenal transposition (SADI-S), intragastric balloon (Balloon). However, there are still some irregular bariatric surgeries in China, which will cause some uncommon complications and bring great pain to patients. At the same time, it is also a huge challenge for regular bariatric surgeons who perform corrective surgeries. This article will review the revision surgery after bariatric surgery in China in the past 20 years, discusses a series of problems caused by non-standard bariatric surgery and the development of regular bariatric surgery.

OBJECTIVE To analyze the antimicrobial activity of recombinant human ?-defensin 3(rhBD-3) on clinically isolated multidrug-resistant bacterial strains.METHODS The antimicrobial activity of rhBD-3 on clinically isolated multidrug-resistant Staphylococcus aureus,Enterococcus faecium,Acinetobacter baumannii and Pseudomonas aeruginosa from the wards of burns department was measured by turbidity method.RESULTS rhBD-3 Demonstrated antimicrobial activity against all the strains in a dose-dependent manner.The minimal inhibitory concentration(MIC) to Gram-positive strains and Gram-negative strains was 4 ?g/ml and 8 ?g/ml,respectively.CONCLUSIONS rhBD-3 Has significant antimicrobial activity against clinically isolated multidrug-resistant strains and thus implies therapeutic potential as an effective substitute for the present drug-resistant bacteria.

Objective To explore the method of obtaining and expanding epithelial stem cells(ESCs) from plucked hair follicle in vitro and clarify that fibroblasts are necessary for ESCs growth.Methods A amount of ESCs from plucked hair were obtained,the epithelial outgrowth of human plucked hair follicle was studied and compared between different culture conditions including no coating well,rat tail-collagen coating well,collagen Ⅳ coating well and 3T3 fibroblasts feeder.The characteristics of these cells were detected by RT-PCR and immunofluorescent staining for CK15.Results After compared between different culture conditions including no coating well,rat tail-collagen coating well,collagen Ⅳ coating well and 3T3 fibroblasts feeder,the results indicated that the plucked hair fragment B3(bulge area of hair follicle) placing on 3T3 fibroblasts feeder was the optimum condition.RT-PCR and immunofluorescent method confirmed that these cells expressed CK15 which was the special marker of ESCs.Conclusion ESCs can be obtained from bulge area of hair follicle which grows on the 3T3 fibroblasts feeder and this method is the optimum condition for ESCs growth after compared between different conditions.

Objective Intranuclear forkhead/winged helix transcription factor p3(Foxp3) plays a key role in T cell-mediated immunosuppression.The present study was performed to investigate the effects of high mobility group box-1 protein(HMGB1) on Foxp3 gene as well as protein expressions in splenic regulatory T cells(Tregs) and their potential regulating mechanisms in mice.Methods CD4+CD25+Tregs isolated from the spleens of male BABL/c mice by magnetic beads were seeded on 96-well(1?105 cells/well) cell culture plates coated with anti-CD3(1 ?g/ml) and soluble anti-CD28(1 ?g/ml),and the cells were stimulated with HMGB1 at various intervals or at different concentrations.After being stimulated,the Foxp3 mRNA/protein expressions in the Tregs were determined.The time-dependent and dose-dependent responses between HMGB1 and intranuclear Foxp3 expression were analyzed by flow cytometry,and the expressions of Foxp3 mRNA of Tregs were analyzed by quantitative PCR of SYBR GREEN.Results After stimulation with HMGB1,the intranuclear Foxp3 protein and mRNA expressions of splenic Tregs in mice were markedly down-regulated in 24 h to 72 h(P