Objective To investigate the relieving effect of acupuncture plus medication on persistent right upper abdominal and scapular pains during acute exacerbation of chronic cholecystitis.Method One hundred patients with acute exacerbation of chronic cholecystitis were randomly allocated to treatment and control groups, 50 cases each. The control group received medication and the treatment group, acupuncture at points Yanglingquan, Dannang and Taichong in addition. Both groups were treated once daily, for a total of seven days. Liver function indicators (alkaline phosphatase (ALP), gamma-glutamyl transpeptidase (GGT), total bilirubin (TBIL) and direct bilirubin (DBIL)) were measured and the VAS score was recorded in the two groups before and after treatment. The clinical therapeutic effects were compared between the three groups.Result There were statistically significant pre-/post-treatment differences in the liver function indicators and the VAS score in the three groups (P<0.05). There were statistically significant post-treatment differences in TBIL and DBIL, and the VAS score between the treatment and control groups (P<0.05). The total efficacy rate was 98% in the treatment group and 50% in the control group; there was a statistically significant difference between the two groups (P<0.05).Conclusion Acupuncture plus medication can effectively relieve the pain induced by acute exacerbation of chronic cholecystitis and improve the liver function indicators in the patients.

Objective To investigate the effects of the 2,3,4′,5-Tetrahydroxystilbene -2-O-beta-D-glucoside on the prolif-eration of PASMCs induced by hypoxia , in orde to search new drugs for the treatment and prevention of hypoxic pulmonary vascular remod -eling.Methods 3%O2 hypoxia was used to induced the proliferation of PASMCs .After hypoxic and TSG treatment for 24h, cell growth was determined by cell counting kit -8 ( CCK-8 ) , cell cycle was analysed by flow cytometry , the mRNA expression of HIF -1αwas measured by quantitative real -time PCR, and the reactive oxygen species ( ROS) production was determined by the fluorescence micro-plate reader .Results TSG can block the proliferation of PASMCs through G 0/G1 to S phase of the cell cycle arrest without cell cytotoxic-ity.Further experiments showed that TSG blocking the proliferation of PASMCs was associated suppression the mRNA expression of HIF -1αand the production of intracellular ROS in hypoxia -stimulated-PASMCs.Conclusion TSG can inhibit the proliferation of pulmo-nary artery smooth muscle cells induced by hypoxia through suppression the mRNA expression of HIF -1αand the production of intracel-lular ROS.

AIM: To compare the effect of s. miltiorrhize bunge injection (SMBI) and s. przewalsii maxim injection (SPMI) on acute focal cerebral ischemia in rats, and to study the mechanism of the actions of two agents. METHODS: Acute focal cerebral ischemia model was made by middle cerebral artery occlusion (MCAO) in rats, and cerebral infarction volume was determined quantitatively by computer microscopical image analysis instruments. MDA content in serum was determined by TBA colorimetric analysis. SOD content in serum was studied by radioimmunity method. The scavenging effect of hydroxyl radical (?OH) was measured by electron spin resonance technique (ESR). RESULTS: SMBI and SPMI could markedly reduce infarction volume after occlusion of middle cerebral artery and MDA content in serum in rats. SMBI could elevate SOD content in serum. Two injections could markedly inhibit the formation of hydroxyl radical (?OH),and the effect of SPMI was better than that of SMBI. CONCLUSION: The effects of both SMBI and SPMI are similar, and it is possible that SPMI as a satisfactory substitute for SMBI.

AIM:To study the effect of levofloxacin(LVFX)on serum concentrations and pharmacokinetics of phenytoin sodium(PHT)in rabbits.METHODS:Using UV-spectrophotometry,the serum concentrations of PHT were determined in nine rabbits before oral feeding LVFS and after a single dose of PHT given iv 10 days later.The pharmacokinetic parameters were worked out with 3p87 program.RESULTS:The concentration-time curve of PHT was fit to open two-compartment model.After co-administration of LVFX,the serum concentrations of PHT were decreased to some extent and part of pharmacokinetic parameters obviously changed as well.CONCLUSION:Co-administration of PHT and LVFX will accelerate the metabolic process of PHT.

Objective To comparative analyze the CT enhanced scan and digital substraction angiography (DSA) in showing the residual and new lesions after treatment of transcatheter arterial chemoembolization (TACE).Methods 60 cases of patients with complete clinical information and imaging data from June 2010 to February 2013 were collected,these patients were diagnosed of primary hepatocelluar carcinoma and underwent TACE treatment.The sensitivity and specificity of CT and DSA to detect the residual and new lesions after TACE treatment were analyzed.By analysis of the main factors affecting the low detection rate to seek a method that can improve PHC residual new lesions detection rate after TACE.Results There were 86 lesions in 60 cases,CT enhanced scan clearly determined the diagnosis of 49 lesions (42 residues,7 new lesions).37 lesions did not prompted to tumor recurrence (residual and new lesions).DSA as the gold standard,the sensitivity of CT enhanced scan to check out the lesions was 84.5 ％ (49/58),specificity was 100.0 ％ (28/28),the false negative rate was 15.5 ％ (9/58),the accuracy was 89.5 ％ (77/86).Enhanced CT detection rate of tumor recurrence was 57.0 ％ (49/86),the DSA detection rate of tumor recurrence was 67.4 ％ (58/86).The difference of determining tumor recurrence between the CT enhanced scan and DSA was significant (x2 =7.11,P ＜ 0.05).Conclusions Three dynamic contrast-enhanced CT scan is the first choice for follow-up examination methods after TACE for hepatocellular carcinoma,but many factors will affect the detection rate of the residual and new lesions.Compared with CT examination,DSA examination has more advantages.DSA examination should be performed when the clinical suspicion of tumor recurrence is negative after enhanced CT scan.

Objective　To study the value and limitation of CT in the damage to multiple organs in children with Langerhans cell histiocytosis (LCH). Methods　Analysis of CT manifestations was performed in 13 cases of LCH proved by clinical features, laboratory data, and pathologic changes. Localized LCH was found in 4 cases, and disseminated LCH in 9 cases. All cases were examined by plain scan in craniocerebral, chest, liver, and spleen, and four cases by contrast-enhanced CT scan. Results　The male outnumbered female in this group. Cranial bones were involved in the sequence of temporal bone(8 cases), parietal bone (3 cases), occipital bone (2 cases), and frontal bone (1 case). CT findings were very sensitive in showing the early destructions of the bones. The characteristic manifestation on CT was multiple lytic lesions of the flat bones at any sites, without reactive sclerosis and soft tissue mass accompanied with the bony lesion. Imaging findings presented later than the clinical manifestations for LCH involving the hypothalamus/pituitary axis. Involvement of the lung at various stages by LCH could be demonstrated by CT scan. Conclusion　Imaging manifestations had no characteristics in LCH involvement. The sites of predilection and related CT manifestations could be suggestive of the diagnosis. In addition, CT findings can be helpful in the classification and evaluation of the therapy and prognosis in LCH.

Objective To evaluate the use of the kite flap (als o known as subcutaneous pedicled V-Y advancement flap)for the repair of moderate -sized anterior lamellar skin defects of eyelid. Methods Since 1994, kite flaps have been used to repair anterior lamellar skin defects of eyelids in 14 patients. Six patients were male and eight female, with a mean age of 43 years (range 15-64 years). The diameter of the largest defect reache d 1/3 length of the eyelid. The flaps were designed adjacent to the defects and the incision line corresponding to natural winkle lines on the eyelid. We underm ined the flap under the orbicular muscle, and advanced the flap to cover the def ect. The incisions were closed with 7-0 suture. Results After operation, all flaps survived with primary healing. Follow-up for 3 to 12 months showed that all cases had achieved satisfactory cosmetic effects without secondary deformity. Conclusions It is a simple, safe, an d reliable method to use kite flap for the repair of moderate-sized anterior la mellar eyelid skin defects that are too large to achieve primary approximation. Because kite-flap covers the defect through a direct advancement way without ro tation and twist, there are no dog-ear deformitys present at the pedical area a nd the repaired area looks smoothly. Because of its widely based muscle pedicle which incorporates venous and lymphatic drainage over most of its length, the un desirable pigment deposition is avoided.

Objective To study the distribution of As and other trace elements in drinking water,grain and soil of arsenism disease area in Tumotezuoqi of Inner-Mongloia and to provide theoretical bases for the prevention and treatment of endemic arsenism and improving water quality.Methods The concentrations of As and other trace elements in well-water,wheat,corn,sorghum and soil were determined using neutron activation analysis.Results The result showed that the arsenic contents in drinking water of Tumotezuoqi were from139to899?g /L,which were much higher than that of the allowance limit(

0.05).The level of TG,TC and LDL-C of high cholesterol group was higher than that of the other groups(P

Objective To investigate whether intestinal bacterial translocation in scalded rat with delayed fluid resuscitation (DFR) was partly due to the reduction of the expression of rat defensin (RD), which was the most important defensin in rat intestinal defensin family excreted by Paneth cells. Methods Fifty-six SPF rats were randomized to 3 groups: sham injury group (n=8); early resuscitation (ER) group (n=24), receiving fluid resuscitation immediately after scald (30% TBSA, third degree); delayed resuscitation group (n=24), receiving fluid resuscitation 6 hours after scald. The animals (n=6, at each time point) were sacrificed at 8, 24, 72 hours after injury. The expression of RD-5 in the terminal ileum was determined with PCR technic. Morphological changes in ileal Paneth cells were observed. Quantitative bacterial cultures of mesenteric lymph nodes (MLN), liver, spleen and lung were also done. Results In both ER group and DR group, ileal RD-5 mRNA expression level was significantly increased at 8 hours after injury, and it began to decrease at 24 hours until the end of the experiment, when the level was much lower than that of the sham group. When two experimental groups were compared, RD-5 mRNA expression level was significantly higher at 8 hours and significantly lower at 72 hours in DR group than in ER group. There were no obvious morphological changes in ileal Paneth cells in all 3 groups. Although high incidence of bacterial translocation was observed in both experimental groups, it was significantly higher in DR group than in ER group. Conclusions DFR did not cause Panth cell damage, and RD-5 changes did not contribute to bacterial translocation in 24 hours after DFR, as the increase in RD-5 mRNA might be an innate protective reaction. However, the decrease in RD-5 production at 72 hours may play a role in bacterial transloca- tion.