Objective To explore the distribution and clinical significance of hepatitis B core antigen( HBcAg) in the hepatocytes of chronic hepatitis B virus infected patients. Methods Paraffin sections were made from 41 liver biopsy samples obtained from chronic hepatitis B virus infected patients. The immuno-fluorescence confocal technique was utilized to analyze the expression level and localization of HBcAg in hepatocytes. The data were analyzed by using Kruskal Wallis test and chisquare test. Results HBcAg expression were detected in 36 (87. 8%) patients, among whom 23 cases had moderate abnormal liver function, 10 with mild abnormal liver function and 3 with normal liver function. Among the cases with moderate abnormal liver function, 6 cases showed the simple membrane-type HBcAg expression, 17 cases showed mixed cytosolic-type and membrane-type HBcAg expression without the nuclear-type expression. Twelve cases with mild abnormal liver function only showed simple cytosolic-type HBcAg expression without membrane-type or nuclear-type expression. In the three patients with normal liver function, HBcAg was expressed in cytoplasm and nuclear but not on membrane. The correlation between HBcAg expression pattern and liver function was statistically significant (χ2 =10. 60, P＜0.01). Conclusion HBcAg expression is directly correlated with liver injury in chronic hepatitis B virus infected patients, which indicates that membrane expressed HBcAg is the target antigen during the immuno-attack of liver.

By reviewing name, type, function and indication of Chinese herbTing-Li-Zi (Lepidii seu Descurainiae) in literatures, there were northernTing-Li-Zi and southernTing-Li-Zi from Song dynasty. It was also known that northernTing-Li-Zi has strong effect and southernTing-Li-Zi had moderate effect. However, there was no strict classification between two types in current clinical practice. The disease prevention and treatment effect of Chinese herbs were depended on their internal features. Disease treatment must be based on syndrome differentiation with corresponding selection of herbs to achieve desired results. Therefore, it is necessary to combine properties with actions in the study of Chinese herbs to provide guidance in clinical practice.

Objective:To explore the role of MVD, bFGF and VEGF in OMFS and the relationship between them. Methods: Immunochemistry streptavidin peroxidase method was used to detect the MVD and the bFGF, VEGF expressions in 57 cases of OMFS samples. Results: MVD, bFGF and VEGF expressions were different between OMFS and benign. The close correlations were found among the expressions of VEGF, bFGF and MVD. Conclusion: The expressions of VEGF and bFGF are associated with MVD which indicated that VEGF and bFGF may play important roles in angiogenesis.

Objectiv e To evaluate the mid-term and long-term clinical outcome of total hip re-pl ace ment in patients with hip congenital dislocation.Methods From June198 3to Decem ber1998,36hips in32patients di agnosed as congenital disloca tion of hip(CDH),aged22to69years old(with the mean of 48.5years), were treated with total hip replacement (THR).Thirty-one hips of 28patient s were fol lowed-up with the duration of 9.5years(3.5to18years).Ac c ording to our system,the congen ital dys-pla sia of hip was divid ed into th ree degrees:the first degree was subluxation in15hips,the second degree was in termedi ate dislo cation in11hips,and the third degree was high dislocatio n in5hips.The fixation of the prosthetic compo nents was as follows :10cup s and12stems with cement,21cups and19stems with un-cement.In acetab ula r preparation,most of the patients with subluxation(13hips)underwent deep ening of the acetabu lum and were installed with larger cups;deepening of the acetabulum,smaller cup and auto femoral grafting on the su perior lip fixed w ith screws were performed in2hips with subluxation and all of hips with interm e di ate and high dislocation.Results The complications included intra operativ e lesser trochanter fracture in1case,deep vein thrombosis and thromboembolism of femoral artery in1case re spec-tively,limb discrepancy in3cases,asepti c loosening in4cases(1with trochanteric nonunion and migration,1with stem l oosening and2with both stem and cup loosening).The Harrisscore was85in th e other27hips(48scores before surgery).Con clusion Total hip replacement in patients with congenital dislocation of hip could be some what difficult be cause of the liability to complications.The techni cal difficulties en coun-t ered during surgery in cluded the correction of the length of bilateral lower extremi ty,the balance of the abductor muscles,the re lease of the soft tis sue,the dealing of the su perior segmental defect of acetabulum and selectio n of the compo nents.

Objective To investigate the effect of nalmefene on the cerebral ischemia-reperfusion (I/R) injury in rats.Methods Forty-eight male Sprague-Dawley rats, aged 3-4 months, weighing 220260 g, were randomly allocated to control group (group C), sham operation group (group S), cerebral I/R group (group I/R), or nalmefene group (group N) using a random number table, with 12 rats in each group.Cerebral I/R was induced by occlusion of bilateral common carotid arteries for 20 min followed by reperfusion.Group C received no treatment.Group S underwent 20 min exposure of bilateral common carotid arteries and then received suture.In group N, nalmefene 0.1 mg/kg was injected intraperitoneally immediately after reperfusion.At 6, 24 and 72 h of reperfusion, venous blood samples were collected for determination of the concentrations of S-100β protein and neuron-specific enolase (NSE) in plasma by enzyme-linked immunosorbent assay.After the last blood sampling, the rats were sacrificed, and brains were removed for determination of interleukin-1beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) contents in brain tissues by enzyme-linked immunosorbent assay.Results Compared with group C, the plasma S-100β protein and NSE concentrations at each time point of reperfusion, and TNF-α and IL-1βcontents in brain tissues were significantly increased in S and I/R groups (P＜0.01).Compared with group S, the plasma S-100β protein and NSE concentrations at each time point of reperfusion, and TNF-α and IL-1β contents in brain tissues were significantly increased in group I/R (P＜0.01).Compared with group I/R, the plasma S-100β protein and NSE concentrations at each time point of reperfusion, and TNF-α and IL-1β contents in brain tissues were significantly decreased in group N (P ＜ 0.01).Conclusion Nalmefene can mitigate cerebral I/R injury in rats.

The article,taking into account basic content of hierarchical theory of needs,human structure theory and mark theory,analyzed the idea and method of procedural education,environmental education and self-governed education for college students.

Objective To summarize the expreiences of management of triplet and quaternion pregnancy mothers and their in our hospital. Methods The clinical data of seven cases of multiple pregnancy (6 case of triplet and 1 case of quaternion) in our department from 1992 to 2003 were analyzed retrospectively. Results All seven patients had middle or severe anemia and hypoproteinemia in second and third trimester. The preterm sign happened in 2/3 cases in 32-36 gestation weeks. The patients were admitted in hospital in 27-34 gestation weeks because of obstetric complication. 6 triplet cases delivered during 35th-37th gestation weeks, except the quaternion one delivered at 33 gestation weeks. There were 22 newborns in those cases, the all babies Apgar's scores were above 9, the birth weights of all babies were above the 3th percent of Chinese singleton gestation and newborn estimated curves, and the birth weights of half babies were above the 10th percent position. Conclusion The key points for managing the multiple pregnancies, especially larger than 3 babies were the following ⑴All pregnant women had a good prenatal care. ⑵Pregnant women rested well, anemia must be corrected, and the adequate nutrition was supplied, especially in third trimester. ⑶Using ultrasound to determine the cervix length and evaluate the risk of preterm delivery, when the preterm delivery sign happened, the inhibiting drugs must be used cautiously and rationally. ⑷ After the antenatal dexamethasone therapy was applied around 36 gestation weeks, selective cesarean section was preferred to deliver the babies. If good prenatal care can be applied, a good outcome for triplets and quaternion can be achieved, close to that of same gestation weeks singleton.

Objective To study the long term results of condylar reconstruction using pedicled patella in the treatment of giant cell tumor of lower femur. Methods From August 1964 to December 1996, 6 patients with giant cell tumor in the lateral condyle of femur were treated with unicondylar resection curettage and reconstruction with pedicled patella autograft. There were 3 males and 3 females. The average age of the pateints was 30.5 years. The operative indications were as the following: tumor was located in one condyle, complicated with pathological fracture or the tumor had invaded underneath cartilage which was difficult for curettage. Surgical procedure included: Lateral condyle resection according to X ray or CT image, curettage of the possible remaining tumor tissues in the medial condyle. A muscle pedicle of 2.5 cm wide from the quadriceps was raised with the patella, then the articular surface of patella was used to reconstruct the articular surface of femoral condyle, ilium graft(sometimes allograft) was used to fill the defect proximal to the patella. The grafts was fixed by screws and(or) plates. Anterior cross ligament was fixed to the patella. Results 6 patients were followed up for a mean period of 136 months. There were no infection, recurrence, metastasis and instability. All patients could walk for a long distance. The range of motion of the involved knees was between 80? and 120?. There was no varus deformity under stress test, but about 5 degrees valgus deformity under stress test could be found in some, muscle strenth was 5 grade. Conclusion Pedicled patella autograft is a good method for the treatment of giant cell tumor that invades unilateral condyle of lower femur, it is specially suitable to the patients with pathological fracture or severe destruction underneath the cartilage. Thorough resection and curretage of the tumor, preserving the blood supply of patella, refixing the cross ligament, and lower placement of patella are the key points to obtain the satisfactory results.

Objective To summarize the achievement and experience in leprosy control in Jiangxi Province and provide scientific basis for formulation of leprosy strategies. Methods Based on the National Leprosy Recording and Reporting System, epidemiological data of leprosy in Jiangxi Province were analyzed using computer. Results Jiangxi Province was a leprosy medium endemic area with a higher distribution of leprosy in the south than the north. The prevalence, incidence and detection rates were highest in the 1959s or 1960s and had decreased annually since then. Average incidence rate for the recent 5 years (0.1604 per 100 000) and prevalence rate (0.049 per 10 000) in 1998 reached the criteria of basic elimination of leprosy at provincial level, however, still 15 counties or cities not reaching the criteria. Conclusion The comprehensive measures including early detection of cases, immediate treatment with multidrug therapy and effective health education are helpful and important for leprosy control.

Objective To evaluate the effect of one stage resection combined with chemotherapy for the treatment of colorectal cancer with hepatic metastasis.Methods In this study 17 out of 36 colorectal cancer patients with hepatic metastasis underwent one stage tumor resection embolization and chemotherapy via hepatic artery and portal vein through pumps in 10 cases. The remaining 19 cases in which metastatic tumors judged as nonresectable received embolization and chemotherapy alcohol injection and electrothermotherapy for the metastatic tumors.Results Recurrence developed in 6 out of 7 patients not receiving primary combined embolization and chemotherapy. The diameter of tumors in the 19 patients decrased by 57% on average, among which 4 patients underwent secondary resection of the metastatic tumors. The 1,2,3 year′s survival rate was 94%, 82%, 65% in combined group, and 74%, 53%, 32%(P