Objective To introduce a method and the clinical effects of treating the blocks of nasal-lachrymal ducts by the memory alloy stands without TV monitoring.Methods 15 blocks of nasal-lachrymal ducts of 12 cases were taken radiography of nasal-lachrymal ducts;to fix the position,to put into the conducting threads from the tear spots and introduce the dilators inversely along the threads.And then China-made titanium-nickel memory alloy stands were planted into the nasallachrymal ducts.Results To visit the patients for 1 to 12 months after operation,the radiography showed that the nasal-lachrymal ducts were clear.The symptoms of overflowing tears and pus disappear completely,11 blocks of 9 cases fully recovered,4 blocks of 3 cases were on the mend.Conclusion Interventional treatment of blocks of nasal-lachrymal ducts is easy,safe and effective.

Objective To investigate the correlation between PaCO2 and cerebral blood flow (CBF), cerebral metabolism of oxygen (CMRO2), glucose (CMRglu) and lactate (CMRlact) and intracranial pressure during intracranial surgery. Methods Twenty ASA Ⅰ - Ⅱ patients (6 male, 6 female), aged between 26-54yr, weighing (65 ? 11) kg scheduled for elective intracranial surgery were studied. The patients were unpremedicated. Before general anesthesia radial artery was cannulated and a catheter was inserted into internal jugular vein and advanced cranially until jugular bulb. Lumber puncture was performed at L3-4 and a catheter was inserted into subarachnoid space for 3 cm, for pressure monitoring and CSF sampling. Anesthesia was induced with diazepam 10mg, fentanyl 3-4?g?kg-1, propofol 2mg?kg-1 and vecuronium 0.08mg?kg-1 iv. The patients were mechanically ventilated with a mixture of oxygen and argon (O2 : argon = 3 : 1) after tracheal intubation. Anesthesia was maintained with sevoflurane and intermittent iv boluses of vecuronium. Arterial and cerebral venous blood gases, glucose and lactate levels, CBF, ICP and CSF level of lactate were determined before anesthesia when patients were awake(Ⅰ) and during anesthesia when PETCO2 = 40, 30, 20 mm Hg (Ⅱ,Ⅲ,Ⅳ). CBF was measured by modified Kety-Schmidt inert gas saturation technique with argon. CMRO2 and CMRglu were calculated based on the difference in their arterial -cerebral venous blood levels. Results At PETCO2 20mm Hg (Ⅳ) CBF decreased by 57.75% and CMRO2 by 58.70% as compared with the baseline; CMRglu decreased by 46.93% as compared with the baseline. There was no significant change in lactate level, jugular venous blood O2 saturation and pH. ICP decreased from (22.14 ? 7.88)mm Hg( Ⅰ) to (17.57?5.03)mm Hg( Ⅱ ),(13.43?4.89)mm Hg(Ⅲ) and (10.00? 2.31)mm Hg(Ⅳ) and the differences were significant. All measurements were done when MAP and HR were stable. PET CO2 was (10? 2) mm Hg lower than PaCO2 . Conclusions Cerebral blood flow, cerebral oxygen and glucose metabolism and intracranial pressure change with changes in PET CO2 . Cerebral vascular reactivity to CO2 is not impaired by 1.3 MAC sevoflurane. Mild hypocapnia is necessary during neurosurgery.

Objective To investigate the effects of mild hypothermia (32.5 - 33.5℃) on neuronal cell apoptosis , Bcl-2 and Bax protein expression in hippocampus following global cerebral ischemia-reperfusion (I/R) in rats. Methods Twenty-four male Wistar rats weighing 250-300 g were randomly divided into three groups of 8 animals : (A) control group-normothermia-sham operation; (B) normothermia-global cerebral I/R (N-I/R); (C) hypothermia-global cerebral I/R (H-I/R) . Global cerebral I/R was produced by occlusion of bilateral common carotid arteries combined with hypotension (MAP = 40mmHg) induced by exsanguinations. Global cerebral ischemia was confirmed by dilated pupils and EEG. Global cerebral ischemia was maintained for 20 min followed by 72 h reperfusion. Mild hypothermia was induced by alcohol spreyed over the rats combined with fanning and maintained at 32.5-33.51 for 3 h. At the end of 72 h reperfusion the animals were sacrificed and hippocampus was obtained for detection of neuronal cell apoptosis (TUNEL) and Bcl-2 and Bax protein expression (immuno -histochemistry technique) . Results The number of apoptotic neuronal cells increased significantly in group B (N-I/R) and C (H-I/R) compared with that in control group (A) (P

Objective:To research the effects and mechanism of Fuganning(FGN) on rats with hepatic fibrosis.Methods:Rat immuno hepatic fibrosis model was induced by intraperitoneal injection of porcine serum,to observe the effect of FGN on HA,LN,CⅣ,the pathological and ultrastructure chages of hepatic tissue.Results:The level of HA,LN,CⅣ decreased,the pathological chages and the collagen fibresis decreased significantly in FGN group,this groups had significanc difference to model group(P

Objective To describe the natural history of cervical intraepithelial neoplasia(CIN)Ⅰand the biologic factors associated with the progression of CINⅠ and to analyze the predictive values of p16INK4a protein for the progression of CINⅠ. Methods From August 2010 to July 2013, 104 patients referred for abnormal cytology [≤ low-grade squamous intraepithelial lesion (LSIL); including negative for intraepithelial lesion or malignancy (NILM), atypical squamous cells of undetermined significance (ASCUS), LSIL] and high-risk (HR) HPV positive,and were diagnosed CINⅠ by colposcopy-assisted biopsy and followed at 1-year intervals in the First Affiliated Hospital of Nanjing Medical University. In order to analyze the relationship between the progression of CINⅠ with clinical biologic factors, including patient age, cervical cytology before colposcopy, loads of HR HPV, HPV L1 capsid protein, p16INK4a protein,χ2 tests was used to compare the different frequencies of factors in groups of progressed and persisted/regressed CINⅠ, then five factors with progressed CINⅠwere processed into binary logistic regression analysis. Results (1) In the first year of follow-up, among 104 patients(including 15 cases NILM,78 cases ASCUS,11 cases LSIL), 52 cases of them were NILM and HR HPV negative, 30 cases were negative for intraepithelial lesion, 10 cases were CINⅠ, 5 cases were CINⅡand 7 cases were CINⅢ. In total, 82 cases (78.8%,82/104) cases had regressed, 10 cases (9.6%,10/104) persisted, 12 cases (11.5%,12/104) progressed [including 5 cases (4.8%,5/104) progressed to CIN Ⅱ, 7 cases (6.7%,7/104) progressed to CIN Ⅲ, none progressed to invasive cancer]. (2) All patients, 88 cases of them accepted immunohistochemical detection the expression of p16INK4a protein. The result shown that 30 cases (34%,30/88) were positive and 58 cases (66%,58/88) were negative. And 94 cases accepted immunocytochemical detection the expression of HPV L1 capsid protein, 49 cases (52%,49/94) were positive and 45 cases (48%,45/94) were negative. (3) Univariate analysis showed that age of the patient, loads of HR HPV, cervical cytology before colposcopy and the expression of HPV L1 capsid protein were not risk factors of the progression of CINⅠ(all P>0.05) except for the expression of p16INK4a protein (P<0.05). Multivariable analysis found that p16INK4a protein positive was associated with progression of CINⅠ(OR=5.1,95%CI:1.162-22.387,P=0.031). (4) Thirty-one cases were p16INK4a protein positive, 8 cases (27%,8/30) of them progressed,while 4 cases (7%,4/58) of 58 cases with p16INK4a protein negative progressed,in which there were significant difference (P<0.05). The sensitivity was 75%, the specificity was 71%, the positive predictive value was 27%and the negative predictive value was 93%for progression to CINⅡ-Ⅲof p16INK4a protein staining. Conclusions The progression rate of CINⅠwith abnormal cytology (≤LSIL) and HR HPV positive was lower, and there was no progression to invasion at 1-year intervals. Immunostaining of p16INK4a protein as the risk factors of CINⅠprogression could have a role in prediction of CINⅠand the management of high-risk CINⅠ.

Linolenic acid containing selenium was synthesized and brought into emul- sion(82). Its aatitumor effects were tested in vitro and in vivo. As a result, when 82 was injected peritoneally into mice(200mg?kg-1?d-1), its inhibitory rate on mouse S180 was 42.6%(P

Background Pregnancy-induced hypertension syndrome (PIHS) appears retinopathy.Optical coherence tomography (OCT) and contrast sensitivity (CS) has been widely used in ophthalmology, but the application of OCT and CS in PIHS fundus lesions is less reported.Objective This study was to assess fundus changes of PHIS by using OCT and CS.Methods Two hundred and thirty-three eyes of 116 PIHS patients with fundus diseases were enrolled from January 2011 to January 2014 in the Cangzhou Central Hospital.One hundred eyes of fifty normal pregnant women were collected at the same period.Best corrected visual acuity (BCVA), fundus examination, OCT, CS were performed on these subjects.The CS values and BCVA between normal pregnant woment and PIHS patients were compared by independent samples t test.The correlation between BCVA grade and CS grade, OCT grade and CS grade, OCT grade and BCVA grade was calculated by trend x2 test and Gamma rank correlation analysis.The study was approved by the Ethics Committee of Cangzhou Central Hospital, all the subjects signed informed consent.Results OCT results showed that 172 eyes were abnormal (65.15％) ,including 94 eyes with neurosensory serous retinal detachment (54.65％) ,40 eyes with pigment epithelium and the ellipsoid zone change (23.26％) and 38 eyes with optic disc edema,retinal hemorrhage and other changes (22.09％).The CS values in each spatial frequency and the BCVA in the PIHS group were significantly lower than those in the normal control group (all at P ＜ 0.05).The OCT examination result was positively correlated with BCVA in the PIHS group (G=0.807,x2=93.418,both at P =0.000).The CS value under the 6.0 c/d had the best correlation with BCVA (G=0.820,x2=97.397, both at P=0.000).The OCT examination result was positively correlated with CS under the 6.0 c/d(G=0.932,x2 =126.376,both at P=0.000).Conclusions Combination of fundus examination,OCT and CS can objectively and comprehensively assess the morphology and function of retinopathy in PIHS patients,which provide the basis for the clinical diagnosis and timely treatment.

Objective To observe the effects of Chinese Traditional Medicine on cognitive functions in the early stage of traumatic braininjury (TBI). Methods 49 inpatients with TBI were divided into treatment group (n=25) and control group (n=24) randomly. The controlgroup accepted routine rehabilitation, while the treatment group accepted Chinese Traditional Medicine in addition. They were assessed withLoewenstein Occupational Therapy Cognitive Assessment (LOTCA) before and after treatment. Results All the scores of LOTCA of treatmentgroup significantly improved after treatment (P<0.01), as well as in control group except "Categories". Most scores of LOTCA improvedmore in the treatment group than in the control group (P<0.05). Conclusion Chinese Traditional Medicine can improve the cognitivefunction after TBI.

Objective To investigate the effects of injection of citicoline into Zusanli (ST36) acupoint on neural function and expression of growth associated protein-43 (GAP-43) in rats with traumatic brain injury. Methods 40 healthy adult male Sprague-Dawley rats were randomly divided into 5 groups: sham-operated group (group A, n=8), acupoint injection of citicoline group (group B, n=8), acupoint injection of saline group (group C, n=8), intraperitoneal injection of citicoline group (group D, n=8) and intraperitoneal injection of saline group (group E, n=8). Opened brain trauma was induced with the modified Feeney method in the groups B, C, D and E, and were treated as design, once a day for 14 days. They were assessed with nervous function score and open-field test before and 8, 14, 15, and 22 days after injury. The expression of GAP-43 in the brain were detected with immunohistochemistry 28 days after injury. Results The nervous function scores and open-field test scores improved more significantly in the group B than in the groups C, D and E (P<0.05). The expression of GAP-43 increased in the group B than in the groups C, D and E (P<0.05). Conclusion Acupoint injection of citicoline into Zusanli may improve the expressions of GAP-43 to promote the recovery of neural function in rats after traumatic brain injury.

Objective To explore the effect of early Chinese medicine intervention on motor function and balance capability of patients with traumatic brain injury (TBI). Methods 55 cases of hospitalized TBI patients were randomly divided into observation group (n=27) and control group (n=28). The control group accepted routine rehabilitation program, while the observation group accepted Chinese medicine therapy in addition for 8 weeks. The motor function and balance capability were tested with Fugl-Meyer Assessment (FMA) and Berg Balance Scale (BBS) respectively before and after treatment. Results The scores of FMA and BBS significantly increased after treatment (P<0.01), and they were higher in the observation group than in the control group (P<0.05). Conclusion Chinese medicine combined with routine rehabilitation program is effective on motor dysfunction after TBI.