1.Determination of Bromate, Iodate, Nitrite in Cosmetics and Foods by Ion Chromatography
Daan DU ; Zhixiong ZHONG ; Jing YAO
Journal of Environment and Health 1992;0(02):-
Objective To establish an IC method for determination of iodate, bromate, nitrite in cosmetics, food and salt by suppressed anion-exchange chromatography and ion-exchange with the addition of post column reagent. Methods A high capacity anion exchange column, Ion Pac9-HC column was used by 9 mmol/L sodium carbonate as eluent, and the flow rate was set up at 1.3 ml/min,an auto-suppression external water mode was used, 0.5 g/L o-dianisidine. 2HCl(ODA)+5 g/L KBr+20%methanel+5.6% nitric acid was used as post column reagent , the flow was set up 0.7 ml/min. The post column reaction (PCR) temperature was 60 ℃ and the visible absorbance detection at 450 nm. Samples were pretreated and analysed. Results The influence of pH of samples and coexist anion on determination was discussed. The detection limit(MDL) of the present method was 0.008-0.10 mg/kg, the recovery rates of the method were 92.0%-109.0% and the relative standard deviation (RSD) were 0.1%-7.2%. Conclusion This method is accurate, sensitive and suitable for trace analysis (?g/kg) of cosmetics and foods samples.
2.Determination of Organic Acids in Cosmetics by Ion-exchange Chromatography Suppressed Conductivity Detection
Zhixiong ZHONG ; Chunsui LIANG ; Daan DU
Journal of Environment and Health 1992;0(04):-
0.999).The relative standard derivations (RSDs) were 0.2%-5.0%, the rates of recovery were 91.1%-108.5%. The detection limits for analysis ranged from 1.3 mg/kg to10 mg/kg. Conclusion The method presented in the paper is sensitive, accurate and suitable for the determination of organic acids cosmetic samples.
3.Determination of Free Fluoride in Toothpaste by Ion Chromatography
Zhixiong ZHONG ; Daan DU ; Xuxia LIANG
Journal of Environment and Health 2001;18(2):104-107
To determine fluoride in toothpaste samples by ion chromatography,the solution of NaHCO3 and the solution of Na2B4O7-NaOH were selected as eluents respectively to optimize chromatographic detection conditions. Either the 0. 45~1.0 mmol/L NaHCO3 solution or the 5.0~20. 0 mmol/L NaB4O7-1. 0~2.0 mmol/L NaOH solution as eluents could effectively separate the chromatographic peak of fluoride in toothpaste samples from acetate and formate and accurately quantify the contents of fluoride in toothpaste samples. The recovery rates,precision(relative standard deviation) and detection limit were 92.5%~100. 2% ,<0. 6% and 0. 208 mg/kg respectively. The data obtained by ion chromatography using NaHCO3 solution as eluent more approached to that by selective-fluoride-ion-electrode.
4.The study of risk factors bringing on death and the conversion of renal function in elder acute renal failure
Xixin WU ; Zhixiong ZHENG ; Yingxiong ZHONG ; Bin TONG
Journal of Chinese Physician 2009;11(4):472-473,476
Objective To investigate risk factors bringing on death and the conversion factors of renal function in the elder acute re-nal failure (ABF) and establish the prevention and treatment measure in clinic to reduce mortality. Methods The clinical information of 143 elder ARF cases which were treated by two hospitals for many years was studied. The risk factors possible bringing on death in the elder ARF were studied by using binary logistic regression pattern. The clinical factors possible affecting the conversion of renal function in the eld-er ARF were analyzed. Results Binary logistic regression pattern study showed that heart failure, respiratory failure, gastrointestinal bleed-ing, hepatic failure, coma, organ failure number and lower blood pressure among 25 study factors were found to be the risk factors bringing on death, and heart failure, respiratory failure, organ failure number and whether chronic renal insufficiency were the most important factors of affecting recover of renal function in the elder ARF. Conclusion The effective prevention and treatment measure should be adopted in the elder ARF with these risk factors so as to reduce mortality and increase the recover rate of renal function.
5.The effects of antiplatelet drugs on aggregation of platelet in patient with acute myocardial infarction
Shuhui HONG ; Junyi HUANG ; Zhixiong LUO ; Weiwen ZHONG
Chinese Journal of Primary Medicine and Pharmacy 2006;0(03):-
Objective To investigate the effect of combined antiplatelet drugs on clinic and aggregation activity of platelet in patient with aucte myocardial infarction(AMI).Methods Consecutive 97 cases with AMI were redomized to receive clopidogrel plus aspirin(treated group,n=48) or aspirin alone(placebo group,n=49) for 30 days.Before and after treatment,the clinical recurrent angina,improvement of heart function,death,ST segment changes of electrocardiogram and the rate of maximun aggregation of paltelet were tested and analysed.Results Recurrent angina occurred in 10.0%(5 cases) in the treated group and 20%(10 cases) in the placebo group.The total frequency of improvement of heart function occurred in 36.7%(18 cases) in the treated group and 22.4%(11 cases) in the placebo group(?~2=3.638,P=0.045).Death occurred in 4%(2 cases) in the treated group and 8%(4 cases) in the placebo group.ST segment of electrocardiogram decreased from (0.36?0.13)mV to (0.13?0.08)mV,compared with placebo from (0.35?0.14)mV to (0.16?0.90)mV(t=3.012,P=0.04).The rate of maximum aggregation of platelet induced by ADP reduced from (74.54?8.99)% to (34.09?9.23)%,the placebo from (72.30?7.78)% to (56.54?6.92)%(t=13.42,P
6.Therapeutic effect of balloon catheter dilatation with ice water on cricopharyngeal achalasia
Juan YANG ; Yinjin SHAO ; Zhixiong XU ; Zhihong LIU ; Ping ZHONG
Chinese Journal of Physical Medicine and Rehabilitation 2014;36(5):363-366
Objective To observe the therapeutic effects of balloon dilatation with ice water and room temperature water on cricopharyngeal achalasia after brainstem stroke.Methods Forty dysphagic patients with cricopharyngeal achalasia after brainstem stroke were recruited.Twenty patients assigned to experimental group were treated by balloon dilatation with ice water and low frequency elecrtrical stimulation.Twenty patients assigned to control group were treated by balloon dilatation with room temperature water and low frequency electrical stimulation.Results After treatment,36 out of the 40 patients of the two groups could eat pasty food independently without choking.Videofluoroscopic swallowing study (VFSS) showed that the cricopharyngeal sphincter relaxed and the foods passed successfully when swallowing bolus,and no aspiration happened.In comparison,the level of cricopharyngeal opening,the number of patients with nasogastric tube remained and eating normally,and the scores of functional oral intake scale (FOIS) between the two groups showed no significant difference (P > 0.05).The average treatment sessions,average hospitalization days and average treatment cost in treatment group were significantly less than those in the control group(P <0.05).Conclusion Cricopharyngeal achalasia can be treated effectively by balloon dilatation with ice water or with room temperature water combined with low frequency electrical stimulation,but treatment course of balloon dilatation with ice water were significantly shorter than that of balloon dilatation with room temperature water.
7.Analysis on current situation of depression and quality of life in patients with advanced gastric cancer and their correlation
Zhi TANG ; Zhennan WANG ; Shuhui LI ; Xianghui LIAO ; Feng XIAN ; Zhong XIE ; Zhixiong LIN
Chongqing Medicine 2016;45(8):1065-1067
Objective To understand the status of depression and quality of life in the patients with advanced gastric cancer , and to explore their relationship .Methods The depression self rating scale (SDS) and the Chinese Scale of World Health Organiza‐tion Quality of Life Scale‐Brief Form Questionnaire (WHOQOL‐BREF) were adopted to conduct the questionnaire investigation on 131 patients with advanced gastric cancer ,then the results were compared with those of the health population .Results Among 131 patients with advanced gastric cancer ,the prevalence rate of depression was 47 .33% ,and the average SDS score was 45 .99 ± 15 .47 ,which were higher than those of the health population .The scores of quality of life were 61 .61 ± 12 .66 for the physiologic health domain ,59 .54 ± 12 .49 for the mental health domain ,64 .95 ± 14 .16 for the social relation domain and 52 .93 ± 14 .07 for the surrounding environment domain .There was a significant correlation between the depression score with the four domains scores of life quality ,in which the correlation with the mental health domain was strongest (r= -0 .636 ,r2 =0 .405) .Conclusion High depression level and poor quality of life exist in the patients with advanced gastric cancer ,and there is a correlation between them , in which the correlation between depression with the mental health domain is strongest .
8.mRNA expression of neuroglobin in thalamus of DAI rats
Zhenyan LI ; Guangwei ZHONG ; Junyu WANG ; Yunsheng LIU ; Sue WANG ; Wei LI ; Zhixiong LIU
Journal of Chinese Physician 2009;11(4):436-438
Objective To study mRNA expression of neuroglobin (NGB) in thalamus of DAI rats and explore the relation between NGB and heed injury. Method 46 male SD rats were randomly divided into four groups. The DAI model was established according to modi-fied Mamamu method. The mRNA expression of NGB in thalamus at different time point after injury was detected by RT-PCR method. All data were analyzed with statistical software. Result The mRNA expression of NGB in thalamus increased obviously from 30 minutes after in-jury while decreased at the 24th hour after injury. But the expression increased again and reached peak at the 48th hour after injury. Conclu-sion The mRNA expression of NGB in thalamus increased after DM. Those changes suggested NGB might participate in the response mech-anism of stress, secondary ischemia and hypoxic brain injury after neuron injury.
9.Construction and identification of chromokinesin KIF4A knockdown gastric cancer cell lines
Kaize ZHONG ; Jian ZHAO ; Pei LI ; Weiwen CHEN ; Changjun ZHU ; Zhixiong DONG
International Journal of Biomedical Engineering 2014;37(1):-
Objective To construct stable chromokinesin KIF4A knockdown gastric cancer cell lines (SGC-7901) and study the mitotic spindle midzone formation in these cells.Methods Short hairpin RNA (shRNA) expression plasmids of pGPU-GFP-shKIF4A,pGPU-GFP-shNC specific targetting KIF4A and non-sense DNA sequences were constructed respectively and then transfected into SGC-7901 cells.The cells were cultured in selection medium containing G418 to form single clones.Stable KIF4A shRNA expressing SGC-7901 cells were picked up under an fluoroscent microscope and identified by Western Blot.The spindle midzone in these cells was analyzed after immunofluorescence staining.Results Three cell lines stably expressing KIF4A shRNA and one with shNC were successfully constructed.Compare to the control cell line,KIF4A knockdown cell lines represented remarkable elongation of spindle midzone and the less the KIF4A,the longer spindle midzone.Conclusions Stable KIF4A knockdown SGC-7901 cell lines were successfully constructed that can serve for further study of KIF4A ' s function and its role in proliferation and development of gastric cancer.
10.Practice and analysis of the research integrity construction at hospitals
Yun CHEN ; Zhixiong ZHONG ; Guobiao ZHANG ; Yuting GUAN ; Xiaole WANG ; Wei ZHONG
Chinese Journal of Medical Science Research Management 2021;34(5):328-331
Objective:To respond to the national requirements on the construction of research integrity, and improve the capacity building of research integrity at hospitals.Methods:Dealing with research integrity problems identified at hospital before 2018, measurements that include " policy and procedures development, standardization of the management of scientific research activities, and optimizing the scientific and technological evaluation system" were adopted to promote the construction of research integrity. Such corrective activities were monitored to summarize experiences and lessons for the continuing improvement of research integrity construction.Results:Both " heteronomy" and " self-discipline" of research integrity have been sublimated simultaneously, at the same time, a tailored research integrity management system with the characteristics of local hospitals has been established.Conclusions:Updating institutional policy and procedures, taking paper management as a priority, as well as research integrity training, these measurements played important roles in promoting the research integrity capacity building at hospitals.