OBJECTIVE:To evaluate the therapeutic effect of Alprazolam on drug-induced intractable hiccup.METHODS:The relevant drugs which induced hiccup were first discontinued and the underlying diseases were actively treated.The trial group received Alprazolam tablets and control group Qiyeshenan tablets.RESULTS:The effective rate was84.4%in trial group and66.7%in control group with significant difference(P

This article describes a Multifunctional Microwave Treatment Instrument.It is an improved model of the general microwave physiotherapy instrument.Equipped with the microwave curative head and adopted a suitable thermostat control circuit,its microwave-transformed thermal energy released from the cura- tive oven and the accessory treatment of certain dose of radiation provide a new way for the surgical treatment of tumors.Besides,the article gives a discussion of the result of the simulation experiment of the instrument. The clinical application of the instrument for the treatment of giioma proves that the instrument enjoys satisfac- tory performance.

BACKGROUND:Stem cel transplantation is increasingly hoped to promote osteoblast differentiation and inhibit osteoclast proliferation in the treatment of osteoporosis.
OBJECTIVE: To study the therapeutic effect of exogenous adipose-derived stem cel (ADSC) transplantation on osteoporosis in ovariectomized rats.
METHODS:Thirty Sprague-Dawley female rats were equivalently randomized into sham, model, ADSC transplantation groups. Rats in al groups except the sham group underwent bilateral ovariectomy to make osteoporosis models. Surrounding adipose tissues instead of the ovary were removed in the sham group. After modeling, rats were given 2×106ADSCs at passage 4via the tail vein in the transplantation group and the same volume of normal saline in the model group, once a week. After 6 weeks, levels of serum calcium, phosphorus, and alkaline phosphatase as wel as bone mineral density and histomorphometry indicators were detected in rats.
RESULTS AND CONCLUSION:Compared with the sham group, the trabecular bone volume fraction was significantly decreased in the model group (P < 0.01), but remarkably increased after ADSC transplantation (P < 0.05). After modeling, the bone trabecular absorption surface percentage and rate of bone trabecular formation were elevated significantly (P < 0.05 orP < 0.01), while these increases were improved by ADSC transplantation (P < 0.05). Additionaly, the levels of serum calcium and alkaline phosphatase and bone mineral density were significantly decreased after modeling, but were increased after ADSC transplantation. In contrast, the serum level of phosphorus was significantly increased in the model group (P < 0.05) but decreased markedly in the ADSC transplantation group (P < 0.05). To conclude, ADSC transplantation can reduce the loss of bone mass in osteoporosis rats by ovariectomy.

Objective To evaluate efficacy of propofol compared with sevoflurane on inflammatory factor and liver function after hepatectomy. Methods 40 cases with hepatectomy were divided into propofol group ( P group) and sevoflurane group ( S group) , 20 cases in each group.The P group and S group respectively were given propofol or sevoflurane maintained anesthesia.The alanine aminotransferase( ALT) , total bilirubin( TBil) , aspartate amino transferase(AST), albumin(ALB), plasma tumor necrosis factor(TNF-α), interleukin-6(IL-6), interleukin-10(IL-10)and complication rate were compared between two groups.Results Postoperation 1d, 3d and 7d, the AST, ALT, and TBiL levels of S group were significantly lower than those of P group (P<0.05).There was no significant difference of ALB level between two groups.The plasma TNF-α, IL-6 and IL-10 levels of S group were significantly lower than those of P group (P<0.05).There was no significant differece of the rate of postoperative complications between S group and P group [6(30.0%) vs.10 (20.0%); χ2 =1.67, P >0.05].Conclusion Sevoflurane inhalation could effectively inhibit the plasma inflammatory cytokines secretion after hepatectomy, and promote liver function recovery, its effect is better than that of propofol.

Objective To study the pretreatment with prostaglandin E1 ( PGE1 ) on acute superior mesenteric ar-tery ( SMA) ischemia reperfusion injury of intestinal cell in rats. Methods 18 healthy male SD rats were randomly divided into control group, sham operation group and experimental group. IRI of SMA model was made by clamping the SMA for one hour and two hours after reperfusion in the control group and the experimental group,respectively. PGE1(20 μg/kg) was injected from the tail vein in the control group and the experimental group. Character of pa-thology of small intestine was examined. The expression of Bax and Bcl-2 in small intestine cells and change of IF-ABP and DAO in serum were detected. Results Pathologic changes showed that there was no change in the control group;while in the sham operation group,chorionic epithelium mucosa ulceration and hemorrhage and necrosis oc-curred more seriously than that in the experimental group. Expression of Bax and Bcl-2 was higher in the sham op-eration group and the experimental group than that in the control group(P<0. 05), and it was obviously lower in the experimental group than in the sham operation group(P<0. 05). The content of IFABP and DAO in blood ser-um:it was higher in the sham operation group and the experimental group than in the control group ( P<0. 05 ) , and it was lower in the experimental group than in the sham operation group(P<0. 05). Conclusion PGE1 can relieve the alvine necrosis caused by rats' mesenteric reperfusion injury after acute artery ischemia and thus protect damaged mucosa of small intestine.

Objective The purpose of this study was to compare the whole body oxygen consumption determined by reverse Pick method and indirect calorimetry in mechanically ventilated patients after cardiac surgery. Methods Eight NYHA II-III patients (2 male, 6 female ) aged 43-68 yr, undergoing aortic valve replacement, mitral valve replacement or coronary artery bypass grafting(CABG) under CPB. Swan-Ganz catheter was placed via right internal jugular vein. Anesthesia and operation were carried out uneventfully. The patients were placed in ICU after operation and mechanical ventilation was continued (IPPV, FiO2 40%-50% , PEEP 5 cm H2O) . PaCO2 was maintained at 35-45 mm Hg by adjustment of VT and RR. T was maintained at 37.0 1C+0.5C .Total body oxygen consumption was measured by the reverse Fick method and indirect calorimetry simultaneously at 2h and 6h after operation. Results The mean oxygen consumption (VO2 ) value determined by indirect calorimetry( 162 + 30 mlmin-1m-2) was significantly higher than that determined by reverse Fick method (127+23 ml min-1m-2)(P

Objective To observe the E cadherin gene abnormality in non small cell lung cancer(NSCLC). Methods E cadherin gene mutations in exons 6, 7, 8 and 9 in 24 cases of non small cell lung cancer were studied by PCR SSCP and DNA sequence analysis. Results Abnormality of the E cadherin gene was found in exon 9 by means of insertion, deletion and mutation in 2 cases. Conclusion Abnormality of E cadherin gene is present in NSCLC by means of insertion and deletion in extracelluar matrix. The main cause of the decrease in epithelial adhesion may be resulted from the abnormality gene change of the E cadherin.

Objective To assess the efficacy of NaClO irrigation of root canal at different temperatures.(Methods Thirty) human teeth with single root-canal mandible premolar were instrumented using standard technique,then were divided into 3 groups,carrying on root-canal irrigation.group A: 5.25% NaClO+System B,group B:5.25% NaClO+15% EDTA,group C:5.25% NaClO+System B+15% EDTA.After the teeth root were split,the scanning electron microscope was used to observe the coronal third,middle third and apical third parts.(Results The) amount of remaining debris on root canal wall in group C decreased significantly,compared with group A and B.The differences of coronal third and middle third between group A and B,group B and C,group A and C were significant(P0.05),but there were significant differences between group A,B and C(P

OBJECTIVE：To explore the possible mechanism of astragalus in improving cardiac function.METHODS：To observe the effects of astragalus,added to basic anti-heart-failure therapy,on cardiac function and TNF in 45 cases of thronic heart failure.RESULTS：In comparison with basic therapy group,the improvement of cardiac function was more obvious and the level of serum TNF-α was lower in astragalus group.CONCLUSION：Astragalus probably has the action of decreasing TNF resulting in improvement of cardiac function.

BACKGROUND:Studies have shown that 26RFa plays an important regulatory role in bone formation, pain, endocrine, cardiovascular disease and energy metabolism. OBJECTIVE:To observe the effects of 26RFa on the proliferation and differentiation of human bone marrow mesenchymal stem cells. METHODS:In order to obtain the most efficient concentration of 26RFa, human bone marrow mesenchymal stem cells were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide analysis. cells were inoculated into 6-wel plates and then divided into two groups:experimental group treated with 10-11 mol/L 26RFa and control group with no 26RFa. After 8, 12 and 16 days of osteogenic induction, alkaline phosphatase activities in induced cells were detected using alkaline phosphatase kit. After 21 and 28 days of osteogenic induction, alizarin red staining and Von Kossa staining were performed. The number of calcified nodules over each coverslip was calculated, and the expression of cbfa1 protein was detected by western blot assay. RESULTS AND CONCLUSION:After 8, 12, and 16 days of osteogenic induction, the alkaline phosphatase activities were higher in the experimental group than the control group (P<0.05, P<0.01, P<0.05). After 21 and 28 days of osteogenic induction, alizarin red staining and Von Kossa staining showed that the number of calcified nodules was higher in the experimental group than the control group, and the expression of cbfa1 protein was also higher in the experimental group (P<0.05). These findings indicate that 26RFa can promote the osteogenic differentiation of human bone marrow mesenchymal stem cells under appropriate culture conditions.