Cytokines involve in many diseases. It' s reported that reduction or increase of cytokines is an important factor to lead to immunological diseases in nervous system. Other studies have found that certain cytokines in the different stages of the same disease play a different role. Understanding the specific mechanisms of cytokines in autoimmune diseases of the nervous system is necessary for providing theoretical basis of new ways to treat these diseases. This article will review the progress of some main cytokines in nervous systemic immunological diseases.

The incidence of carotid stenosis disease increases with age,and it is one of the major causes of ischemic stroke.In recent years,with the rapid development of endovascular treatment technology,the therapeutic means,including carotid angioplasty and stenting,has been widely carried out both at home and abroad.This article reviews the advances in endovascular treatment of carotid stenosis.

Objective To introduce the recent studies about the lymph node-targeted chemotherapy for gastric carcinoma. Methods The literatures on the lymph node-targeted chemotherapy for gastric carcinoma in recent years were collected and reviewed. Results The lymph node-targeted chemotherapy for gastric carcinoma was effective because it couled improve the drug concentrations in regional lymph node. Conclusion As a part of multiple treatments for gastric carcinoma, lymph node-targeted chemotherapy will be further developed.

Objective To investigate the inhibitory effect of cyclooxygenase-2 (COX-2) inhibitor celecoxib alone and in combination with somatostatin (SST) analog octreotide on human gastric cancer. Methods Fifty one gastric cancer patients were randomly divided into 3 groups. The patients in control group(n=15)took no medicine before gastric cancer resection. Celecoxib group(n=18)patients took celecoxib orally 0.2 g/d for 7 days before surgery. Combination group(n=18) patients took celecoxib orally 0.2 g/d and were injected octreotide 100 ?g/d subcutaneously for 7 days before surgery. The resected specimens were examined histologically, including status of tumor necrosis, inflammatory cells infiltration and fibrous tissue proliferation. The microvessel density (MVD) and the expression of COX-2 in gastric cancer were evaluated by immunohistochemical methods. The apoptosis of tumor cells was measured by terminal deoxynucleotidyl transferse-mediated-dUTP nick end labeling. The expression of somatostatin receptor (SSTR) -1,SSTR-2 and SSTR-3 mRNA in gastric cancer tissue obtained by biopsy in all patients were detected by using real time fluorescence quantitative RT-PCR. Results Compared with control group, gastric cancer tissue necrosis increased significantly(P

Objective To observe the effect and study intervenient action of Kuijieling Granules (KG) in treating ulcerative colitis (UC) during the active period by the clinical experiment. Methods The patients of active UC (damp-heat syndrome) were randomly divided into 2 groups (KG+SASP group and SASP group) to observe pathological changes of the mucosa,syndrome effect and main symptoms scores,and the protein expression of TLR4,CD14 and NF-?B p65 were detected by immunohistochemistry. Results KG+SASP group had better effect than SASP group in syndrome and mucosa (P0.05). There were outstanding differences among the pathologic grade of UC about IA of TLR4,CD14 and NF-?B p65. The expression of TLR4,CD14 and NF-?B p65 were decreased by treatment (P

Objective To establish the animal model of damp-heat syndrome(DHS) and explore the expression of aquaporin(AQP) 3,4 gene in gastric mucosa.Methods Thirty SD rats were randomized into 3 groups and observed for 20 days.Group A(normal group,NG) was fed with routine method.Group B(Pi deficiency syndrome group,PDS) was fed alternatively with routine method and aqua of cassia angustifolia Vahl.Group C(DHS) was fed with fat,honey liquid and treated with damp-heat environment.The expression of AQP 3,4 gene in the 3 groups were determined by fluorescence quantitative polymerase chain reaction(FQ-PCR).Results The symptoms and signs in Group C were simiar to the clinical DHS.Expression of AQP3 gene in DHS was statistically significant higher than that in PDS and NG(P

Aim To establish a method of hydrodynamic-based transfection(HBT) and provide a means in rats of the gene therapy of liver diseases,which allowed an efficient expression of rIL-10 gene in rat liver.Methods Using rIL-10 gene as a reporter gene,different volumes and doses of plasmid DNA solutions were rapidly injected into rat tail vein,then the serum and the tissue of liver,kidney,lung,spleen and heart in different time were collected and the expression of rIL-10 was detected by the methods of RT-PCR,ELISA and immunochemistry.Results Using rIL-10 gene as a reporter gene,the results demonstrated that an efficient transfer and expression of rIL-10 in rat liver could be achieved by a rapid injection of a large volume of rIL-10 DNA solution into rat via tail vein.Maintaining a stable expression of rIL-10 in serum could be assessed by repeated administration.Conclusion The HBT was a simple,convenient and efficient method of gene transfer and expression in rats,which could be used as an effective means to study further gene therapy of rIL-10 in liver diseases.

Objective:To analyze the characteristics and differences of upper airway forms in the adults with class Ⅱ sagittal facial type of different vertical facial types and investigate the correlation between craniofacial structures and upper airway forms.Methods:Cephalograms of 135 adults with classⅡsagittal facial type of different vertical facial types were collected.60 cases were males and 75 cases were females.All the cephalograms were measured in sagittal upper airway depths.The differences were compared between genders,and also among different vertical facial type groups.Results:Among different vertical facial type groups,only the sagittal depths of nasopharynx(PNS-Ba、PNS-R)of the males and PNS-Ba of the females in high angle groups were smaller than that in average and low angle groups(P0.05),but the values of V-LPW of the males were larger than that of the famales in all vertical facial type groups(P

A high-throughput method for screening drug multi residues was developed by quadrupole-time-of-flight( Q-TOF) mass spectrometry together with QuEChERS sample preparation technique. It has been used for the determination of 57 drugs, such as tetracyclines ( TCs ) , sulfonamides ( SAs ) , quinolones ( QNs ) , triphenylmethanes (TPMs), estrogens (ETs), androgens (AGs) and glucocorticoids (GCs) in fish. The optimized pretreatment conditions were examined. The target compounds were extracted with acetonitrile under the condition of Na2 EDTA-Mcllvaine buffer, and the usage of clean-up reagents was 25 mg anhydrous magnesium sulfate, 12. 5 mg octadecylsilane and 6. 25 mg N-Propylethylenediamine sorbent for extracted solvent of each milliliter. The positive results acquired by this high-throughput method were confirmed by liquid chromatography-tandem mass spectrometry ( LC-MS/MS) . The detection limit for these 59 drugs was in the range of 0. 5-5. 3 μg/kg. The method is time-saving, convenient, effective and wide coverage. Its sensitivity can meet the requirement of the detection of drug residues in aquatic products.