Objective To explore the main X-ray feature of the non-metallic foreign body in lower respiratory tract in order to improve the diagnostic accuracy.Methods X-ray findings of non-metallic body in lower respiratory in 150 cases proved by tracheobronchoscopy were retrospectively analyzed.The changes of heartshadow,mediastinum pulmotransparency and diaphragm during the procedure of deepbreath were observed under fluoroscoopy in different position,based on that the location of foreign body was evaluated.Results The distribution of foreign bodies in all patients included:38 cases in trachea,81 cases in right bronchus (25 cases in right primary bronchus,4 cases in upper lobar bronchus,5 cases in middle lobar bronchus,47 cases in lower lobar bronchus and segmental brouchus),31 cases in left bronchus (13 cases in left primary bronchus,2 cases in upper lobar bronchus and 16 cases in lower lobar bronchus).Because of the anatomical difference,the foreign bodies usually saw in right lower lobar bronchus.Conclusion Those patients who were suspected with foreign bodies in lower respiratory tract should be routinly examined by X-ray including fluoroscopy which is of important value in localized diagnosis of non-metallic foreign body in lower respiratory tract.

Objective To improve the recognition of diabetic pedipathy through analyzing the X-ray findings of 20 cases.Methods Total 850 patients of diabetic mellitus were collected since 1985, of them,there were 20 cases with the complications of osteoarticulation or soft tissue of foot. The X-ray findings combined with clinical information were analysed.Results X-ray characters included:(1) osteoporosis in 20; (2) symmetrical atrophy of diaphysis in 14;(3) osseous absorption and destruction on the distal end of the bones in 11;(4) osteoarthritis in 7;(5) Charcot articulation in 2;(6) soft tissue swelling in 20, 5 of them having calcified vessels in area of metatarsus and phalanx.Conclusion For the patieuts with long-term diabetes without regular treatments, with repeating infection and even developing to obstinate ulceration on foot, the possible of diabetic pedipathy should be concerned. In order to obtain early diagnosis and effective therapy, it is necessary to take X-ray examination.

Objective:To evaluate whether oral administration of heat shock protein60(HSP60) could induce immune tolerance and its effect on the progression of aortic atherosclerotic plaque in hypercholesterolemic apolipoprotein(apo) E-/-mice.Methods:8 week-old mice were divided into two groups that were orally administrated PBS plus HSP60 and only PBS separately for 5 days,and then a high-cholesterol diet was started 2 days after the last treatment for 12 weeks.Pathological analysis of plaque was performed,and percentage of CD4+CD25+regulatory T cells in the splenocytes was analyzed,proliferation response of the splenocytes to HSP60 was detected and cytokines in the superanant were measured.Results:Compared with control animals,oral tolerance to HSP60 resulted in a significant decrease of atherosclerotic plaques in size.Tolerance to HSP60 induced a significant increase of CD4+CD25+ cells in the spleen.Specific proliferation response of splenocytes to HSP60 was significantly suppressed and TGF-? in the superanant increased while IFN-? decreased significantly.Conclusion:HSP60 specific tolerance can be induced via oral administration of HSP60 which in turn attenuates progression of plaque.This provides a new immunologic approach for treatment of atherosclerosis.

Objective To explore the in vivo killing effects of nanosecond pulsed electric fields on mouse breast cancer in tumor bearing BALB/c mice. Methods Twenty four BALB/c mice models with subcutaneous EMT-6 breast cancer were prepared and divided into experimental group and control group (n=12). In experimental group, the mice were treated by the pulsed electric fields with peak voltage of 700 V/cm, rise times of 57 ns, durations of 70μs and duty ratio of 50%on the tumor tissue with two parallel electrodes under anaesthesia. One hundred pulses were given and the energy was just 9.75 J with effective action period of 7 ms. Tumor volume, animal weight and histopathologic changes after 1, 3, 5 and 7 d were observed after irreversible electroporation. Results The tumor volume in experimental group increased more slowly than that in control group, especially at the 1-3 d after treatment with a standstill. Histopathological examination results showed that the tumor tissue had obvious coagulation necrosis and tissue edema after electrical stimulation treatment, and mass vacuole like structure appeared in tumor tissue from the third day after treatment and vascular injury occurred at the early stage. Conclusions In the given field conditions, steep pulsed electric field has inhibitory effect on tumor growth. In order to achieve more ideal tumor therapy effect, further studies on steep pulse dose are needed.

Objective: To establish a cell culture model of cervical-loop epithelial cells from Wistar rat lower incisors on culture bottle coated with rat tail collagen. Methods: The effect of self-made rat tail collagen on the culture of cervical-loop epithelial cells was observed. The cells were identified by immunohistochemistry staining. Results: Cervical-loop epithelial cells in Wistar rat lower incisors grew well in self-made rat tail collagen. The cervical-loop epithelial cells exhibited positive expression for integrin-β1 and monoclonal antibody CK in immunohistochemistry staining. Conclusion: The cell culture model of cervical-loop epithelial cells in Wistar rat lower incisors with self-made rat tail collagen can be helpful to research tooth development mechanism.

Objective To study the effect of thermo-radiotherapy on multidrug resistance (MDR) and levels of intracellular adriamycin (ADM) in tongue squamous cell carcinoma cell line Tca 8113 and its MDR cell line Tca 8113/CBDEA. Methods Samples of the two cell lines were treated with thermo-radiotherapy (42℃ for 0.5 h and 2 Gy of radiation). Four and 24 hours later, the expression of the MDR relative proteins P-glycoprotein (P-gp),multidrug resistance associate protein 1 (MRP1) and glutathione s-tranferase-π (GST-π) were detected using immu-nohistochemistry. Intracellular ADM concentrations were measured using an HTS 7000 Plus bioassay reader. Re-sults No change in the expression of P-gp was observed in the Tca 8113/CBDEA and Tca 8113 cell lines after 4 or 24 hours. Expression of MRP1 was not significantly altered in the Tca 8113/CBDEA cell line, but there was a signifi-cant drop in the Tca 8113 cell line 24 hours post-thermo-radiotherapy. Expression of GST-π was not altered in either the Tca 8113/CBDEA or the Tca 8113 cell line at 4 hours post-thermo-radiotherapy, but there was a significant de-crease at 24 hours. At both 4 and 24 hours, drug tolerance had decreased and intracellular drug concentration had in-creased significantly in both cell lines. Conclusions Thermo-radiotherapy can enhance the effects of chemotherapy and suppress the expression of MDR factors induced by radiation. The combination of hyperthermia and radiotherapy does not induce MDR.

Objective: To explore influence of sleep deprivation (SDP) on serum interleukin-1β (IL-1β) level in rats with coronary atherosclerosis (CAS). Methods: A total of 96 healthy SD rats were randomly and equally divided into normal control group, CAS group, SDP group and SDP + CAS group according to number table; rats in CAS group and SDP + CAS group were fed with high cholesterol diet to establish CAS animal model; SDP group and SDP + CAS group received SDP through small platform with water environment. Radioimmunoassay was used to measure serum concentration of IL-1β; and serum concentrations of total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) were measured respectively; above indexes were compared among four groups. Results: (1) Compared with serum IL-1β level of normal control group, there were significant increase in SDP group, CAS group and SDP + CAS group[(13.33±3.20) ng/L vs. (14.40±4.41) ng/L vs. (22.21±5.94) ng/L vs. (30.17±7.45) ng/L] (P<0.05～<0.01); (2) Levels of TC, TG and LDL-C in CAS group and SDP + CAS group were significantly higher, level of HDL were significantly less than those of normal control group and SDP group (P<0.05～<0.01), levels of above indexes in SDP + CAS group were significantly higher or less than those of CAS group (P<0.05 all). Conclusion: Sleep deprivation can significantly heighten serum interleukin-1β level and total cholesterol, triglyceride, low density lipoprotein-cholesterol levels, decrease HDL level in rats with coronary atherosclerosis or sleep deprivation.

Objective To observe the effect of Huoxue-Yixin decoction combined with atorvastatin on NT-pro and QTd in patients with chronic heart failure (CHF). Methods A total of 120 patients with chronic heart failure in our hospital from December 2016 to June 2017 were enrolled in this study. The subjects were randomly divided into the control group (n=60) and the treatment group (n=60). The control group were treated with conventional treatment,and the treatment group were treated with Huoxue-Yixin decoction combined with atorvastatin. The two groups were treated for 30 days. The NT-ProBNP, QTd, QTcd and LVESD, LVEDD, LVEF of the two groups before and after treatment were compared.Results The QTd at 2 d(45.6 ± 8.9 ms vs.64.0 ± 18.2 ms,t=-7.029),14 d(44.3 ± 6.8 ms vs.55.7 ± 12.8 ms,t=-6.092)in the experiment group were significantly lower than those of control group(P<0.01).The level of QTcd at 2 d(50.8 ± 10.1 ms vs.71.2 ± 19.8 ms,t=7.109), 14 d(48.1 ± 9.7 ms vs.63.1 ± 13.1 ms,t=7.128)of the experiment group were significantly lower than those of control group(P<0.01).After treatment,the NT-ProBNP(494.58 ± 331.61 ng/L vs.594.71 ± 382.65 ng/L,t=-1.532), LVESD(40.23 ± 3.13 mm vs.49.72 ± 3.99 mm,t=-14.495)and LVEDD(58.22 ± 3.30 mm vs.65.11 ± 3.95 mm, t=-10.720)of the treatment group were significantly lower than those of the control group(P<0.01).The LVEF (69.82% ± 4.72% vs. 50.34% ± 4.02%, t=-10.720) of the treatment group was significantly higher than the control group(P<0.01).Conclusions The Huoxue-Yixin decoction combined with atorvastatin can reduce the level of NT-ProBNP, QTd and QTcd in patients with CHF, can significantly improve cardiac function.