Objective To compare the effect of low-temperature plasma ablation and YAG laser treatment on persistent allergic rhinitis(PAR) after septoplasty and to seek adjunct surgical procedures to tackle PAR.Methods Follow-up was performed three years after randomized grouping on PAR patients ( Low-temperature plasma ablation group 127 cases and YAG laser group 1 18 cases).Visual Analog Scale (VAS) was used to evaluate symptoms,such as nasal congestion,running nose,sneezing,nasal itching,eye itching etc preoperatively and postoperatively in order to compare the treatment effectiveness and intergroup difference.Results The efficacy rate of Low-temperature plasma ablation group was 87.4％,and YAG laser group was 66.1％.The difference was statistically significant( x2 =15.74,P ＜ 0.01 ).The VAS scores for postoperative symptom relief in both groups were significantly different ( t =21.24,20.56,all P ＜ 0.0 1 ).Conclusion As an adjunct treatment approach,low-temperature plasma ablation was superior to YAG laser in the treatment of PAR with preferable safety.

Surface plasmon resonance (SPR) sensing technology is a high-tech optical detection technolo-gy developed quickly in recent years, which combines biology, polymer chemistry and sensing technologies to form a rapid, sensitive, specific, portable and easy to operate detection technology. This paper outlines the mech-anism of SPR sensing technology for detection of low molecular weight analytes, the main application methods and research progress, discusses the advantages and shortages of the method, and foretastes the development prospect of this technology.

0.05). Conclusions Ultrasonography is an objective, accurate, non-invasive and utility method to evaluate the saphenous artery of saphenous skin flap before operation.

Objective To investigate the changes of monoamine neurotransmitters of the brain in physical dependence induced by morphine in rats.Methods A physical dependent rat model was established with morphine in a gradually in creasing doses and the withdrawal syndrome was scored after naloxone precipitation.The conditioned place preference(CPP)in rats induced by morphine was used to investigate psychic dependence in rats.Contents of norepinephrine(NE0,dopamine(DA)and serotonin(5-HT)in hypothalamus of rats were assayed with a fluorescent method. Results (1)In naloxone-precipitated withdrawal test of morphine-dependent model rats,after morphine had been withdrawn,morphine-abstinent rats presented marked withdrawal symptoms and signs,their withdrawal scores were significantly increased,and the levels of NE and 5-HT in the rat brain were obviously enhanced,but the content of DA was reduced.(2)In CPP test,morphine caused a marked place preference in rats and the levels of DA and 5-HT in the rat brain were obviously enhanced,but the content of NE was reduced.Conclusiion Morphine dependence development and withdrawal are closely connected with monoamine neurotransmitters in CNS.In the physical dependent model induced by morphine in rats,the rising of NE and 5-HT in the rat brain were significant,but in psychic dependent model induced by morphine in rats,the levels of DA in the rat brain were enhanced p-redominately.

OBJECTIVE: To study on clinical significance of acoustic rhinometry and nasal resistance checks by low temperature radiofrequency ablation on nasal endoscopy operation. METHOD: Application instrument of acoustic rhinometry and nasal resistance meter on 106 cases with moderate to severe persistent nasal obstruction symptoms. These patients are not obvious or invalid after regular drug treatment. The preoperative and postoperative six months, with the nasal mucosa contraction before and after by inspect respectively,also record nasal resistance (NR), nasal minimum cross-sectional area (NMCA), distance of the minimal cross-sectional area to the nostril (DCAN), mean nasal cross-sectional area (MNCA), nasal cavity from volume (NCV). The other 36 cases of healthy people as a control group. Before and after nasal mucosa contraction by inspect. Two sets of data were statistically analyzed with SAS6.12. The efficacy evaluation of radiofrequency ablation by improve the extent and visual analog scale (VAS) score, with the patient's subjective symptoms. RESULT: The group of preoperative rhinitis treatment NR higher than the controls significantly, the NMCA significantly lower than the control group (P< 0.01). The group of postoperative by radiofrequency ablation and rhinitis treatment was 100%, postoperative VAS scores were decreased compared with pre operative (P < 0.01). The group of rhinitis treatment NR was significantly lower than the preoperative, and NMCA significantly increased compared with the preoperative (P < 0.01). Before and after nasal contraction in the same group, NR and NMCA was no difference (P > 0.05). The NR and NMCA of the postoperative in rhinitis treatment group was no difference compared with the control group (P > 0.05). CONCLUSION Low temperature radio frequency ablation by nasal endoscopy operation had a significant improvement of nasal ventilation functions for patients with moderate to severe chronic rhinitis, acoustic rhinometry and nasal resistance can be an objective and accurate evaluation to this operation.
Adult ; Airway Resistance ; Case-Control Studies ; Catheter Ablation ; methods ; Endoscopy ; Female ; Humans ; Hypothermia, Induced ; Male ; Middle Aged ; Nasal Obstruction ; physiopathology ; surgery ; Nasal Surgical Procedures ; methods ; Rhinitis ; physiopathology ; surgery ; Rhinometry, Acoustic ; Treatment Outcome ; Young Adult

Objective To investigate the correlation of the middle cerebral artery stenosis (MCAS) and the mild cognitive function impairment (MCI),and the clinical efficacy of statins in patients with MCI. Methods Six hundred and thirty-six patientse,who received transcranial color doppler ultrasound (TCD)assay, were enrolled in our hospital hospitalization or outpatients. The simple mental state examination (MMSE) and clinical dementia rating scale (CDR) were used as cognitive function assessment indexes. Forty-four cases of MCI with MCAS and 58 cases of MCI with NMCAS were used as the treatment group , who received the atorvastatin 20 mg every day , 56 cases of MCI with NMCAS were used as the control group , who only received the routine and basic diseases treatment. One yearlater,we determined the changes of MMSE and CDRagain. Results We detected 124 patients with MCAS, 512 patients with NMCAS, and 44 cases of MCAS patients with MCI, the prevalence was 35.5%,114 cases of NMCAS in patients with MCI, with the prevalence of 22.3%, the prevalence between the two groups was statistically different. One year later, the patients in the treatment group, MMSE score was improved, the score of MCI of the MCAS group improved more significantly. Conclusion The middle cerebral artery stenosis correlated with the occurrence of MCI. Atorvastatin could improve cognitive function in patients with MCI, especially for MCI which was caused by middle cerebral artery stenosis.

BACKGROUND:hIL-24, a tumor suppressor gene, can stimulate immune responses, inhibit the growth of tumor cel s, and the formation of tumor vessels, and induce cel apoptosis. OBJECTIVE:To explore the effects of hIL-24 gene on the proliferation and apoptosis of fibroblasts in the keloid and the underlying mechanisms. METHODS:Al the keloid specimens col ected from 13 patients were used for fibroblast culture and indentification. Fibroblast of the keloid was transfected with or without hlL-24 lentivirus. Subsequently, mRNA expressions of transforming growth factor-β, Smad3, proliferating cel nuclear antigen, matrix metal oproteinase-2,-9, and metal opeptidase inhibitor 1 were determined. RESULTS AND CONCLUSION:Immunofluorescent staining and flow cytometry showed that vimentin antibody was expressed positively in cytoplasma of fibroblast cultures, and the purity was more than 97.8%. Western blot assay showed that hIL-24 expression was significantly increased in the transfected fibroblasts. Quantitative PCR showed that the overexpression rate of hIL-24 in fibroblasts was 81.7%and mRNA expressions of transforming growth factor-β, Smad3, proliferating cel nuclear antigen, matrix metal oproteinase-2, and-9 were significantly decreased, while metal opeptidase inhibitor 1 mRNA expression was significantly increased in hIL-24 transfection group compared with control group (P<0.05). These findings suggest that hIL-24 gene inhibits the expressions of proliferating cel nuclear antigen, matrix metal oproteinase-2, and-9 in fibroblasts, and the underlying mechanism may involves TGF-β/Smad3 pathway.

Objective To study the effect of Ro60 on migration and invasion of lung adenocarcinoma A 549 cells. Methods We knocked down Ro60 and analyzed the ability of migration and invasion of A 549 cells.Quantitative real time ( RT)-PCR and Western blotting were performed to detect mRNA and protein levels of selected molecules associated with migration and invasion of neoplasm .Results When Ro60 was downregulated , the migration and invasion of A 549 cells decreased markedly.Meanwhile,the mRNA expression of matrix metalloproteinase (MMP)9,c-Src etc was observably reduced.Furthermore, downregulation of Ro60 diminished the expression of Src protein and the activation of MMP-9 protein.Conclusion Downregulation of Ro60 inhibits migration and invasion of A549 cells by regulating Src protein and activating MMP-9 protein.

Objective: Enriching secoiridoid glycoside in Gentiana manshurica kitag. Methods: Gentiana manshurica kitag. was extracted with 70% EtOH by cold macerating and purified with AB 8 macroreticular resin. Results: The content of gentiopicrin in the extract is 28.69%. Conclusion: AB 8 macroreticular resin fits in purification of water soluble secoiridoid glycoside in Gentiana manshurica kitag.

Objective To investigate behavior and hippocampal cytoskeletal alterations following re-exposure to chronic unpredictable mild stress(CUMS) and acute swimming stress,and explore the possible mechanism.Methods 40 Male Sprague-Dawley (SD) rats were divided into five groups,with 8 exposed to 21 consecutive days of chronic unpredicted mild stresses (CUMS) and treated with vehicle,8 exposed to CUMS and treated with fluoxetine,8 exposed to CUMS and treated with fluoxetine and re-exposed to acute swimming stress after washout,8 exposed to CUMS and treated with fluoxetine and re-exposed to CUMS after washout,and 8 as normal controls treated with vehicle.Behavioral changes in these rats were analyzed.The expressions of hippocampal cytoskeletal microtubulin were analyzed using Western Blot.Results ( 1 ) Compared with the control and CUMS group,sucrose preference (43.38 ± 7.84 ) ％,total traveling distance ( 859.21 ± 653.62 ) cm,velocity ( 2.05 ± 0.60 ) cm/s and frequencies of rearing(0.12 ±0.30) were reduced (P＜0.01 ) in the re-exposure to CUMS group.After re-exposed to acute stress,these behaviors did not differ from control rats.(2)Compared with the control and CUMS group,the Acet-Tub expression (244.24 ± 8.90 )％ of re-exposure to CUMS group showed a significant increase (P＜ 0.01 ) in rats submitted to re-exposure to CUMS and Tyr-Tub expression (30.92 ± 11.01 )％ was significantly decreased following re-exposure to CUMS (P＜0.01).At the same time,MAP-2 expression did not change while phospho-MAP-2 expression (24.75 ± 8.83 )％ decreased significantly.After re-exposed to acute stress,these prorein expressions did not differ from control rats.Conclusion These findings provide evidence that rats re-exposed to CUMS showed more impairment of cytoskeletal microtubular dynamic and structural neuronal plasticity,this effect appears to be mediated by the degree of phosphorylation of MAP-2.