Objective To quantify the percentage of CD8+ T cells and their expressions of cytotoxic molecules and homing-related chemokine receptors in peripheral blood from patients with atopic dermatitis (AD).Methods Peripheral blood was obtained from 15 patients with AD and 14 healthy controls.Flow cytometric analysis was performed to determine the percentages of CD8+ T cells and CD8+CLA+ T cells in the peripheral blood samples,as well as the expression levels of cytotoxic molecules and homing-related chemokine receptors on these cells.Differences in these parameters were analyzed using t test,and relationship between these parameters was evaluated using Pearson correlation coefficient.Results No significant difference was observed between the patients with AD and healthy controls in the percentage of CD8+ T cells,the expressions of perforin,granzyme B,CCR10,CCR6 or FasL on CD8+ T cells,or the expressions of CCR4,CCR10,CXCR6 or FasL on CLA+CD8+ T cells (all P ＞ 0.05).A significant increase was noted in the percentage of CLA+CD8+ T cells (3.80％ ± 1.46％ vs.2.18％ ± 0.85％,t =3.636,P ＜ 0.01) and expression rates of CCR4 on CD8+ T cells (13.86％ ± 4.42％ vs.9.50％ ± 2.14％,t =3.738,P ＜ 0.01) as well as perforin and granzyme B on CLA+CD8+ T cells (74.27％ ± 15.94％ vs.57.20％ ± 14.64％,t =2.998,P ＜ 0.01; 70.90％ ± 13.85％ vs.56.41％ ± 11.00％,t =3.104,P ＜ 0.01) in the patients with AD compared with the healthy controls.Conclusions The proportion of CLA+CD8+ T cells is increased with enhanced expressions of cytotoxic molecules such as perforin and granzyme B in peripheral blood of patients with AD,which may contribute to the pathogenesis of AD.

OBJECTIVE: To find the characteristic of tympanogram results obtained from normative neonates 2 - 7 days about low frequency (226 Hz) probe tone, and to determine the normative values for tympanometric variables. METHOD: Transient evoked otoacoustic emission (TEOAE) screening were performed by using AccuScreen Pro instrument for 135 neonates. Among them, 105 neonates passed the TEOAE screening in both ears. The 226 Hz probe tone tympanograms and their normative values were obtained from them using GSI-33 middle ear analyzer. RESULT: The 226 Hz tympanometric data for the 105 neonates (210 ears) passed the TEOAE screening in both ears showed a double-peaked tympanogram in 202 ears (96.19%), a single-peaked tympanogram in 8 ears (3.81%). For double-peaked tympanogram in 202 ears, the tympanometric normative values was below: the first peak admittance is (0.91 +/- 0.18)mmho, Tpp is about (18.02 +/- 12.26)daPa; the second peak admittance is (1.05 +/- 0.23)mmho, Tpp is about (-35.05 +/- 16.80) daPa; the admittance of notch between the two peaks is (0.74 +/- 2.57)mmho, its pressure is about (0.37 +/- 7.61) daPa. Vec is about (0.50 +/- 0.08)ml. CONCLUSION The 226 Hz tympanograms obtained from this cohort may serve as a guide for evaluating middle ear function in neonates.
Acoustic Impedance Tests ; methods ; Acoustic Stimulation ; Evoked Potentials, Auditory, Brain Stem ; Female ; Humans ; Infant, Newborn ; Male ; Neonatal Screening ; Otoacoustic Emissions, Spontaneous ; Reference Values