Urinary system often requires the restoration and reconstruction due to the impairments resulting from the congenital abnormality or acquired damage. Previously, some of autologous tissues are used as the substitutes of the restoration, but they are unsatisfactory regarding the complicated sampling, limited resources and many complications, as well as the inefficient structure and function of original tissues and organs. With the development of tissue engineering techniques, the tissue-engineered scaffold materials have provides a novel method for the restoration and reconstruction of urinary system. According to the sources, the tissue- engineered scaffold materials can be divided into artificially synthesized scaffolds, native scaffolds and extracellular matrix materials. Native scaffold materials carry good biocompatibility and low antigenicity, but their sources are little. Artificially synthesized scaffold materials are excellent in the physico-mechanical property, but some of them such as polylactic acid are absent to the specific information recognized by the cells, thus losing the cellular affinity. In contrast, extracellular matrix materials are better in the biocompatibility for the local urinary tissues. However, they are observed for only a short term, further researches needs to focus on the side effect or adverse reaction in vivo. In this study, three kinds of tissue-engineered scaffold materials have been introduced and their application characteristics in the restoration and reconstruction of urinary system are also analyzed, as well as their application prospect.

BACKGROUND:Vascular grafts made of expanded polytetrafluoroethylene have been shown to have highly hydrophobic surface characterized by resistance to water penetration and biological durability. OBJECTIVE:To investigate the effects of vascular grafts made of expanded polytetrafluoroethylene for repair of peripheral arterial injuries. METHODS:Thirty-eight patients with peripheral arterial injuries, consisting of 30 males and 8 females, aged 17-61 years, were included in this study. After conventional fluid infusion, anti-shock treatment, anti-inflammation, and debridement, they received implantation of vascular grafts made of expanded polytetrafluoroethylene. A 12-month folow-up observation was performed to investigate the recovery of affected limbs and vascular patency in patients. RESULTS AND CONCLUSION:After implantation of vascular grafts made of expanded polytetrafluoroethylene, al vessels were unblocked and no cases died or had limbs amputated. Blood supply of al affected limbs recovered to normal and there was no vascular anastomotic infection. Imaging results showed that vascular patency rate was 100%. Ultrasound examination results showed that at the end of the folow-up period, the inner wal of the vascular grafts was smooth, obvious mural thrombus or anastomotic stenosis did not occur in any case, vascular patency rate was 92% (35/38), and no adverse reactions related to artificial vessels were observed. These findings suggest that vascular grafts made of expanded polytetrafluoroethylene for repair of peripheral arterial injuries exhibit striking surface anticoagulant property and long-term patency.

Purpose:To evaluate the relationship between po stoperative survival rate and three stage systems (Nevin,AJCC,JSBS) of gallbladd er carcinoma. Methods:From October 1988 to December 1998 a total of 30 patien ts with gallbladder carcinoma underwent radical resection in the Xinhua Hospital . All patients were classified by 3 different methods of staging.The 1-year,3- year ,5-year postoperative survival rates were evaluated according to the stage . Results:All patients with stage Ⅰ were alive. There were less cases in Nevin’s stageⅡ,Ⅲ. The 3-year,5-year survival rates in patients wit h Nevin’s stage Ⅱ,Ⅲ were better than the other two classifications. The 5-ye ar survival rates in patients with Nevin’s stage Ⅲ were better than those with Nevin’s stage Ⅳ. The 3-year,5-year survival rates in patients with Nevin’s stage Ⅳ/Ⅴ were significantly better than the other two classifications (P

Gallbladder cancer is the most common malignancy of biliary tract with a very poor prognosis. The therapeutic strategy of gallbladder cancer has been improved in some aspects. Identifying the accurate stage is the basis of surgical treatment. Radical resection is the only choice of treatment which provides patients long survival. For most patients in T1a stage, simple cholecystectomy is adequate, but dissection of hepatoduodenal ligament should be added when the lesions located in neck and duct of gallbladder. Patients in T1b stage often need radical cholecystectomy. Radical cholecystectomy with Ⅳb and Ⅴ segmentectomy and lymphadenectomy of N2 nodes should be performed to patients in T2 stage. Extended right lobe resection can improve the prognosis of selected T3 and T4 patients.The effect of chemotherapy for gallbladder cancer remains unsatisfactory, and current chemotherapeutic regimens were based on 5-FU, gemcitabine or S-1. The effect of a new chemotherapeutic sensitization scheme with continuous infusion of somatostatin,epirubicin 5-FU/CF and cisplatin is under research, and the preliminary results are promising. Radiation shows some benefits to patients with gallbladder cancer, but its effects are still uncertain.

Recently, heme oxygenase-1 (HO-1) has been emphasized in the area of intensive care unit (ICU) and other domains. HO-1 can catalyze the decomposition of heine and the products of its enzymatic activity, including carbon monoxide, biliverdin, and bilirubin and ferritin can play a significant cytoprotec-tive role in antagonizing inflammation, protecting cells from oxidative injury and cellular stresses. In the re-search of severe acute pancreatitis (SAP), researchers have realized that it is of great importance to antago-nize the overflow of cytokines in order to improve the therapeutic effect of SAP. In this article, we give a re-view of the research progress of HO-1 and its relationship with SAP.

One case of secondary bleeding repeatedly after tonsillectomy. Secondary bleeding after tonsillectomy seldom happen to us. When the event occurs medical staff has to take effect way to control the bleeding immediately. Accumulated quantity of bleeding must be controlled to the lowest point as soon as possible. Other wise the bleeding may lead to death. This point should be considered by medical staff.
Humans ; Postoperative Hemorrhage ; etiology ; Tonsillectomy ; adverse effects

Objective: To prepare a functional chitosan thermosensitive hydrogel for minocycline-HCl gelatin microspheres and observe its effects on repairing experimental rats gingivitis. Methods: Minocycline-HCl gelatin microspheres were prepared by using gelatin as core material and emulsion crosslinking method, then the latter was incorporated into the chitosan thermosensitive hydrogel. Established an experimental gingivitis model in SD rats and to observe the effect of hydrogel on gingivitis. Results: Microspheres thermosensitive hydrogel had obviously sustained effects on gingivitis. Results showed that GI,PD in experiment group were significantly improved. Conclusion: The chitosan thermosensitive hydrogel loading of minocycline-HCl gelatin microspheres have antiphlogistic effects on experimental rats gingivitis.

AIM:To study the microRNA profiling in the serum of insulin-resistant mice and the mechanism of insulin resistance induced by related microRNAs.METHODS:A high-fat diet was used to induce insulin resistance model in KM mice.The microRNA profiling in serum of insulin-resistant and normal mice was analyzed by microarray chip and were validated by real-time PCR.miRanda data base was used to forecast target genes.miRBase was used to obtain the se-quences of related microRNAs, based on which protein interactions were predicted using the online analytical tool STRING. RESULTS:In serum of insulin-resistant mice, the expression of miR-125, miR-126, miR-143, miR-30a, miR-199a, miR-127, miR-184, miR-30e, miR-134, miR-195, miR-206, miR-429, miR-212, miR-362, miR-382, miR-154 and miR-466h was significantly up-regulated.miR-211, miR-504, miR-877 and miR-1930 were significantly down-regulated. miR-143 associated with insulin resistance was able to bind to 3'-UTR of fat mass and obesity-associated protein (FTO), and FTO was found to interact with Rpgrip1l, Tmem18, Mc4r, Npy, Hhex, Tcf712, Cdkal1, Slc30a8, Igf2bp2 and Tha-da.CONCLUSION:Twenty-one microRNAs in the serum of insulin-resistant mice induced by a high-fat diet are signifi-cantly different from those of normal mice, in which 17 kinds were significantly up-regulated.miR-143 closely related to in-sulin resistance is able to regulate FTO protein expression, which interacts with other 10 proteins associated with the occur-rence and development of diabetes.The results are also useful for further study of the molecular mechanisms in insulin re-sistance.

Objective To compare the impact of conventional vs. zero-balanced ultrafiltration on serum pro-inflammatory factors,acute kidney injury and clinical prognosis after cardio-pulmonary bypass procedure. Methods Forty patients receiving cardiac surgery under cardio-pulmonary bypass procedures in Xiangyang Central Hospital during January 2013 to June 2013 were randomly divided into conventional ultrafiltration group(group A,n=20)and zero-balanced ultrafiltration group(group B,n=20). Blood and urine samples were collected on different time points( pre-operation,T0;pre-ultrafiltration,T1;immediately after ultrafiltration,T2;24 hours post-operation,T3;48 hours post-operation,T4;7 days post-operation, T5). TNF-α,IL-6,KIM-1,CysC,serum creatinine and urea nitrogen were detected and compared. Pre-and post-operative clinical data were also collected. Results There was no difference in baseline data or intra-operative data(p>0. 05). TNF-αand IL-6 started to increase when the operation began. Compared with conventional ultrafiltration,zero-balanced ultrafiltration alleviated the increase of TNF-α and IL-6,espe-cially on T2,T3,and T4(p<0. 05). Zero-balanced ultrafiltration also decreased CysC,Rbp,serum creati-nine and urea nitrogen on T2,T3,and T4 compared with conventional ultrafiltration(p<0. 05). A total of 11 patients experienced AKI( >grade I)in group A and 2 patients experienced in group B(p<0. 01). There were significant differences of ventilation time,total complication incidence and ICU stay time be-tween two groups. There was no difference in other complications,post-operative days in hospital or death rate within 30 days. Conclusion Though there is a trend of more patients receving renal replacement therapy,no statistical difference has been achieved. In conclusion,zero-balanced ultrafiltration can effec-tively decrease the concentration of serum pro-inflammatory factors,alleviate acute kidney injury and improve the clinical prognosis after cardio-pulmonary bypass procedures. It is a safe and reliable method valuable for promotion.

Objective To study the effect of Sirtuin1 (Sirt1) on the pathological process through its activity of deacetylation to improve the clinical outcome of acute sepsis. After searching data base, microRNA-211 (miR-211) was found to have action potentially targeting at Sirt1.The present study aimed to find the interaction between miR-211 and Sirt1 in the pathogenesis of hypoxic injury to cardiomyocytes in the presence of lipopolysaccharide ( LPS ) . Methods Primary neonatal rat cardiomyocytes ( NRC ) isolated from neonatal SD rats and H9c2 ( cardiomyocytes after culture with 10% fetal serum of cattle and DMEM under 37 ℃ and 5% CO2 ) cell line were used in the experiments.The miR-211 expression was quantified by qRT-PCR after LPS exposure for 4 hours, and the changes in Sirt1 protein level were also detected in both NRC and H9c2 by western blot.At the same time, CCK-8 assay and TUNEL staining were also performed to measure cell proliferation and apoptosis activation when either treated with LPS alone or followed by exposure to hypoxia.Results Compared to the control group, the doses of 20μg/mL, 40μg/mL LPS treatment for 4 hours had no significant effects on H9c2 cell proliferation at 24 h, 48 h, 72 h, but it could significantly induce the cell apoptosis of neonatal rat cardiomyocytes and H9c2 cells after hypoxia, and the apoptosis rate increased all over 100% in both NRC and H9c2.At the same time, LPS treatment could significant up-regulate miR-211 expression which was closely associated with decrease in Sirt1 protein levels.Conclusions LPS enhanced cardiomyocytes injury after exposure to hypoxia which was closely associated with up-regulating miR-211 expression and in turn to suppress Sirt1 expression.