Objective: To investigate the expression of α-B crystalline in triple-negative breast cancer (TNBC) and discuss its role in the prognosis and targeted therapy of TNBC by analyzing the correlation amongα-B crystalline and the clinico-pathological indi-ces of breast cancer. Methods:Immunohistochemistry SP assay was used to determineα-B crystalline expression in paraffin-embedded specimens of 30 cases of TNBC and 50 cases of non-TNBC tissues. The differences in protein expressions among tissues were com-pared, and correlation analysis was performed to determine the relationship between protein expression and clinico-pathological indi-ces. Results:The positive expression rate ofα-B crystalline was 66.67%in TNBC (20/30) and 42.00%in non-TNBC (21/50), which in-dicates that the expression is significantly higher in TNBC than in the non-TNBC tissues (χ2=4.566, P<0.05). The expression ofα-B crystalline in breast cancer has no significant difference in terms of age, tumor diameter, and cancer stage (P>0.05), but exhibits a differ-ence in terms of lymph node metastasis and the number of metastasized lymph nodes [P53, Ki67 (P<0.05)], which presents a positive correlation (r=0.251). Theα-B crystalline expression in TNBC has no significant difference among indices such as age, tumor diameter, nodal metastasis, number of metastasis, P53, cancer stage, and Ki67 (P>0.05). Conclusion:Significant differences were found inα-B crystalline expression between TNBC and non-TNBC.α-B crystalline is highly expressed in TNBC tissues, and the expression is signif-icantly higher in TNBC tissues than that in non-TNBC tissues.α-B crystalline is associated with poor prognosis of breast cancer. Conse-quently, the overexpression ofα-B crystalline in TNBC may be a reason for unfavorable TNBC prognosis.

Objective:To study the expression of Ki67 and P53 proteins in gingival tissue after wearing four kinds of temporary crowns.Methods:The experiment animal model was established by temporary prosthesis on dogs' teeth using chemical-curing resin,thermal-curing resin,DMG-TEMP crown material and SWIFT composite resin respectively.The immunohistochemistry Envision method was used to measure the expression of Ki67 and P53 proteins in gingival tissue before tooth preparation,after tooth preparation,1-and 2-week,1 month after wearing the crowns.The teeth without any treatment were surved as control.HE stained gingival tissue slices were observed under microscope.Results:The expression of Ki67 and P53 proteins in gingival tissue increased 1 week after wearing the chemical-curing resin crowns or thermal-curing resin crowns(P0.05).No pathological change was observed in all the samples.Conclusion:Chemical-curing resin crowns and thermal-curing resin crowns may increase the expression of Ki67 and P53 proteins in gingival tissue,but DMG-TEMP crowns and SWIFT resin crowns do not.None of the four kinds of temporary crowns may cause abnormal proliferation of gingival epithelia.

Objective To study the relationship between the retinal arteriosclerosis prevalence and related factors in in-service teachers from 3 universities. Methods The medical report of in-service teachers from 3 universities from July to October, 2012 were analyzed. Results and Conclusion 2680 cases were included, in which 520 cases (19.4%) were diagnosed as retinal arteriosclerosis. Retinal arteriosclerosis prevalence increased with age and cardiovascular risk factors. In the multi-factor logistic regression analysis, gender, age, hypertension,hyperlipidemia, impaired fasting glucose regulation, diabetes, hyperuricemia are independent risk factors for retinal arteriosclerosis

ObjectiveTo observe the effect of recombinant human interleukin-11 combined with ciclosporin in treatment of giucocorticoid invalid idiopathic thrombocytopenic purpura.MethodsFrom August 2009 to August 2011,35 cases with glucocorticoid invalid idiopathic thrombocytopenic purpura patients were divided into two groups according to the treatment method:13 cases in control group were treated with ciclosporin and 22 cases in observation group were treated with recombinant human interleukin-11 at basis of above method.The clinical effect and platelet counts between two groups were compared.Results The total effective rate in observation group was 90.9％ (20/22),which was significantly higher than that in control group [53.8％ (7/13)] (P ＜0.05).After treatment,the platelet counts in both two groups were significantly increased [control group:( 112.5 ± 15.4) × 109/t vs.(13.2 ± 1.8 ) × 109/L; observation group:( 132.7 ± 22.3 ) × 109/L vs.(12.9 ± 1.6) × 109/L] (P ＜ 0.05 ).Moreover,the platelet counts after treatment in observation group was significantly more than that in control group (P ＜ 0.05).ConclusionRecombinant human interleukin-11combined with ciclosporin in treatment of glucocorticoid invalid idiopathic thrombocytopenic purpura is a good treatment scheme,which can be applied in clinic.

Objective To establish an immature rabbit model of avascular necrosis of the femoral head in-duced by exercise. Methods Ten male, immature New Zealand white rabbits were subjected to large range, in- tense passive movement and concentric impingement on their right hips for 4 weeks. The left hips were used as self-controls. Then X-ray and magnetic resonance imaging, gross anatomical observation and histological examination were used to evaluate avascular necrosis of the femoral head. Result After 4 weeks, avascular necrosis of the femoral head was successfully replicated. Increased bone density, decreased osteoepiphysis height and indistinct bone trabec-ula were found in X-rays of the right hips. In MRI images obvious joint hydrops could be detected in all right hips, and schistic low signal areas in the femoral head could be seen in TIWI and T2WI images. Thin bone trabeculae of low density, with irregular and broken structures, were also found in H-E sections. Conclusion An immature rab-bit model of avascular necrosis of femoral head can be successfully induced through large range, intense, passive movement and concentric impingement.

Objective To explore the activity of Elemene for glioma cell from the cellular and molecular level. Methods The human glioma cell U251 was cultured. The effect of Elemene for human glioma cell proliferation was studied by MTT assay. Cell cycle, Fas, PCNA, bcl-2, intracellular Ca~(2+) and apoptosis were evaluated by flow cytometry analysis. Results Elemene exhibited antiproliferative effect on human glioma cell U251 markedly. The fifty percent inhibition on concentration (IC_(50)) of Elemene against glioma cells at different time points. 24 h was 40.60 μg/ml, the 48 h 38.14 μg/ml and the 72 h 34.35 μg/ml.Cell cycle was blocked in the S and G_2/M phases. The apoptosis ratio was increased by Annexin V staining markedly. Elemene decreased the gene expressions of PCNA and Fas, increased the intracellular Ca~(2+). There was no significant effect on the bcl -2 gene expression. Conclusion Elemene exhibits a marked antiproliferative effect on glioma cells and induces apoptosis by decreasing the expression of PCNA and increasing intracellular Ca~(2+). It also influences the expression of Fas. It might have no relationship with bcl-2 gene expression.

Objective To demonstrate the cause of visual loss (VL) caused by pituitary adenomas (PAs),through retrospective study on the magnetic resonance imaging (MRI) radiological materials of patients with PAs.Methods Forty eight patients with PAs were selected,who received surgical treatment at our Hospital.Their medical records and MRI radiological films were reviewed.The relationship between the characteristics of PAs and the visual deterioration were analyzed.Neuro-ophthalmological findings were elevated with the relationship between the tumor and the supraelinoidal segment (C2 segment) of the internal carotid artery(ICA).Resuits On the coronal MR images,C2 segments of 28 sides(29.2%) were involved by the tumors whose value of VL in the ipsilateral eyes averaged 0.80±0.20 (range from 0.40 to 1.00).In the rest 68 sides (70.8%) without C2 segments involved,the valHe of VL in the correspondent eyes averaged 0.30±0.30 (range from 0.00 to 1.00).The difference was statistically significant (P<0.05).Conclusions If the C2 segment of ICA is invaded,the worse visual acuity would appear.which might be due to the markedly involved chiasmal arteries.

Objective To investigate the differential diagnosis of Reye syndrome and the characteristics of primary carnitine deficiency,and to provide diagnostic strategy for similar cases.Methods There was a case presented with fever,poor response,convulsions and hepatomegaly hospitalized in Wuhan Children's Hospital,and the clinical manifestations were described,the physical examination was comprehensively conducted,the auxiliary examination results were recorded,some pediatric specialists from ICU,neurology department,genetic metabolic department,digestive system department were invited to discuss the case.The treatment was adjusted according to the suggested opinions;the treatment effects and the final diagnosis were tracked.Results The primary diagnosis of the case was central nervous system infection or toxic encephalopathy at the time of admission,but Reye syndrome could not be excluded.Although the cerebrospinal fluid test and brain MRI examination detected nothing abnormal,liver function suggested alanine aminotrans ferase ALT increase,blood sugar decrease,the liver volume increase,which was detected by liver ultrasound.Blood amino acids examination revealed the carnitine level decreased,and it was confirmed as primary carnitine deficiency in the end.L-carnitine was used to treat the disease,and its effect was good.Conclusions Great importance should be attached to children with onset age,physical check-up,and multidisciplinary cooperation.Use monism to explain the illness and the auxiliary inspection as far as possible,so that it can get early diagnosis and treatment,and the outcome is good.

AIM:To observe the effect of Huayu Qutan Decocion on patients with cerebral infarction. METHODS: 63 patients with cerebral infarction were randomized into treatment group and control group.The treatment group was given symptomatic therapy and Huayu Qutan Decoction.The control group was given symptomatic therapy and Aspirin tablets.After 14 days of treatment,the changes in score of the nerve function defect grades,the ability of activities of daily living and blood viscosity were observed. RESULTS: After a comparison between two groups,the symptomatic improvement of treatment group was more better than that in control group (P

Objective To compare the reliability of modificatory method and traditional method in preparing ischemic stroke rat model in rehabilitation period.Methods 173 female SD rats were divided into traditional method group(n=63),modificatory method group(n=109) and control group(n=37).In the traditional method group,the thread embolus was inserted through the left external carotid artery(ECA),and then left ECA and pterygopalatine artery(PPA) were ligated.In the modificatory method group,the thread embolus was inserted through the left internal carotid artery(ICA) and only left common carotid artery(CCA) was ligated.In the control group,the thread embolus was not inserted,only CCA was ligated.Results There was no significant difference in neurofunction score between the modificatory method group and the traditional method group(P>0.05).The operating time was significantly shortened(P<0.01) and the survival time was significantly prolonged in the modificatory method group compared with that of the traditional method group(P<0.01).The two-month survival rate was 54.13% in the modificatory method group and 31.75% in the traditional method group receptively,the former was significantly longer than the latter(P<0.01).Conclusion The ischemic stroke model established by modificatory methods is not only precisely and reliable but also save operating time and improve survival rate of the animals compared with the traditional method.The main death causes of animals are large-area cerebral edema and electrolyte imbalance after operation.