60mg /kg?d ip, of paeonol were given to rats for 15 days. The myocardial ischemia reperfusion injury model was produced by occluding the left coronary artery and releasing the occlusion in rats. This significantly decreased myocardial Ca++-ATPase activitics and CH/PL radical, FFA content, improved mitochondrial membrane fluidity and kept them away from oxygen free radical damage. These results indicated that paeonol has membrane protective effect on myocardial ischemia rcperfusion injury probably by inhibiting the oxygen free radicals and subsequent lipid peroxidation.

Objective To compare the differences of infect rates and genotype distribution of 21 kinds of human papillomavir-us (HPV)in women cervical cells in Quanzhou and Wuhan regions.Methods Collected gynecological clinic of 10 068 women in cervical cell specimens from November 2012 to January 2014 in Affiliated Quanzhou First Hospital of Fujian Med-ical University and Renmin Hospital of Wuhan University,of which 6 823 cases in Quanzhou,Wuhan region 3 245 cases were detected to identify HPV genotypes by using nucleic acid hybridization technique(HybriMax).Results 2 439 in 10 068 samples of cervical cells were detected to be infected HPV with overall infection rate of 24.23%(2 439/10 068).The overall HPV infection rate in Quanzhou was 22.61% (1 543/6 823),and 27.61% (896/3 245)in Wuhan.The single HPV infection rate in Quanzhou was 17.28% (1 179/6 823),and 19.88% (645/3 245)in Wuhan.The multiple HPV infection rate in Quanzhou was 5.33% (364/6 823),and 7.73% (251/3 245)in Wuhan (The values of P<0.05).The five most common HPV genetypes in Quanzhou was HPV-16,HPV-52,HPV-58,HPV-11 and HPV-CP8304,respectively,in Wuhan was HPV-16,HPV-52,HPV-58,HPV-33 and HPV-18,respectively.Conclusion The overall,single and multiple HPV infection rates in Wuhan were all markedly higher than those in Quanzhou (the values of P<0.05).The most common HPV gene-types in two regions were different.

Objective The purpose of this study was to understand the pathogenic mechanisms of enterococcal hemolysin and establish the foundation to diagnose and control relevant diseases.Methods Take 30 clean grade ICR mice were randomly divided into ten groups,each 6,1 to 4 groups according to 10-fold gradient amount per 1 ml intraperitoneal injection of 5× 107 cfu/ml~5×1010 cfu/ml of EF A7,group 5 as a control group injected 0.5 ml saline,take another 30 clean grade ICR mice,intraperitoneal injection of 5×107 cfu/ml~5×1010 cfu/ml of EF A7 cyl mutant by the same measures,compared to the original strains of mice infected with mutant strain LD50,and a week after infection mortality was observed in mice daily. Another seven groups of mice with the same bacterial concentration (5×109 cfu/ml)were injected intraperitoneally EF A7 and EF A7 cyl mutant,after infection 6 h,12 h and 24 h comparing the number of leukocytes in peripheral blood of mice,re-spectively,the concentration of TNF-αin the acute phase of cytokines.Results In the mouse peritonitis models the 50% le-thal dose (LD50)of EF A7 cyl mutant was 100 times lower than enterococcus faecalis EF A7.The differences of the survival percentage of EF A7 cyl mutant groups and EF A7 groups have significance (P<0.05).As the changes in boold leukocytes numbers and the TNF-αlevel.Conclusion Cyl gene is probably a major virulence factor of Enterococcus and plays an impor-tant role in the mouse peritonitis model.

Objective To evaluate the short-term outcomes of minimally invasive percutaneous plate osteosynthesis(MIPPO) combined with locking compression plate(LCP) in the treatment of distal tibial fractures.Methods From June 2004 to March 2006,16 patients with distal tibial fractures were treated using MIPPO combined with LCP in our department.According to the AO classification,there were 7 cases of type 43A1,5 type 43A3,2 type 43B1 and 2 type 43C3.After reduction of tibial fracture with three methods,LCP was inserted and fixed with locking screw.All the cases were followed up periodically after the operation.Results All the cases were followed up for 5 to 20 months(mean,11.5 months).Primary wound healing was achieved in 16 patients.No complications,such as non-union,abnormal union and breakage of the plate,occurred in the cases.Callus formation was seen on X-rays at 7.6 weeks(4 to 12 weeks) after the surgery,when the patients began part weight-bearing exercises.The bone union was detected at 16 weeks(8 to 20 weeks),when the patients began full weight bearing.3 patients felt uncomfortable at the distal tibia around the implant.According to the AOFAS Ankle-Hindfoot Scale,14 cases(87.5%) were excellent and 2(12.5%) were good.Conclusions MIPPO combined with LCP technique is an effective treatment for the fractures of the distal tibia.This minimally invasive method can achieve stable fixation,promote bone healing,and permit early functional rehabilitation.

Objective To investigate the clinical significance of 18 F?FDG PET/CT in assessment of Tomita score in spinal metastases. Methods From October 2012 to October 2015, a total of 77 patients (38 males, 39 females;average age:56.5 years) with suspicious spinal metastasis tumors underwent 18F?FDG PET/CT scan and were included in this retrospective study. Spinal metastasis were confirmed by pa?thology. Each patient was evaluated by the scoring system of Tomita according to the grade malignancy of pri?mary lesions and metastasis of organs and bones in PET/CT. Results Seventy?two patients were osteolytic metastases. Among them, only 23 patients received operative procedure. There were 2 patients with osteoblas?tic metastases, and 3 patients with both osteolytic and osteoblastic lesions. The average Tomita score of opera?tion group was 5.7. Two to three scores were assessed in 2 patients, 4-5 scores in 9 patients, 6-7 scores in 9 patients, and 8-10 scores in 3 patients. The average score of non?operation group was 8.8. One case got 2-3 scores, 2 patients got 4-5, 18 patients got 6-7, and 33 patients got 8-10. Conclusion PET/CT is a convenient and effective tool for assessment of Tomita score in patients with spinal metastases.

Objective To evaluate the curative effects of surgical treatment of unstable clavicular fractures. Methods From August 2000 to April 2005, 50 cases of clavicular fracture in our department underwent open reduction and internal fixation with reconstruction plate or clavicular hook plate or 1/3 tubiform titanium plate. According to Robinson’s classification, 12 of them were identified as type-2B1, 24 as type-2B2, 1 as type-3A1 and 13 as type-3B2. 13 cases of them had other injuries. 37 patients were fixated with a reconstruction plate. Clavicular hook plates were applied in 12 cases. One patient was fixated with the 1/3 tubiform titanium plate. The curative effects were evaluated on the basis of healing time, quality of healing and function of the shoulder. Results All the 50 patients were followed up. The mean follow-up was 19 months (2 to 57). All the clavicular fractures achieved bony union. The mean healing time was 2.5 months (2 to 4). 3 patients experienced malunion. The functions of the shoulder were excellent in 32 cases, good in the other 18 patients. The total clinical efficacy was evaluated as excellent in 22 patients, good in 23 and fair in 5. Conclusions The type-2B and type-3B fractures in Robinson’s classification belong to unstable clavicular fractures. The patients treated by operation can recover normal functions soon without considerable complications.

Objective To discuss the indications, surgical techniques and outcome of less invasive stabilization system (LISS) in treatment of special proximal femoral fractures. Methods Twenty-eight patients with special proximal femoral fractures were treated with LISS from June 2005 to December 2007. All fractures could not be reduced by close reduction with traction table. There were eight patients with in-tertrochanterie fractures ( including type 31-A2.2 fractures in three, type 31-A2.3 in two, type 31-A3.1 in three and type 31-A3.3 in two according to AO classification), 19 with subtrochanteric fractures (including type ⅡA fractures in three, type ⅢB in one and type Ⅴ in 15 according to Seinsheimer classification) and one with peripheral fractures ( type Vancouver B1 fracture) of the femoral prosthesis. The data about opera-tion duration, intraoperative blood loss, postoperative complications, fracture healing time and postoperative hip function score were recorded. Results The operation lasted for mean 88 minutes (45-120 minutes), with intra-operative blood loss of average 320 ml (50 -700 ml). There occurred deep vein thrombosis of lower limb in two patients, myocardial infarction in one and lower digestive tract bleeding in one after oper-ation, with no incision complications. One patient with breast cancer was died of brain metastasis after op-eration and three lost follow-up. Other 24 patients were followed up for mean 18 months (6-33 months), which showed that 23 patients got fracture healing 2-5 months ( average 3 months) postoperatively but that one patient with type Seinsheimer V fracture had breakage of proximal locking screws six months postopera-tively because of nonunion of subtrochanteric fracture. The Harris score of the hip was 70-99 points ( aver-age 84 points). Conclusions Reverse femoral LISS has advantages of minimal invasion, easy opera-tion, stable fixation, high degree of safety and minor complications for treatment of special proximal femoral fractures. Skillful reduction, correct positioning of guide wire into hole A and avoidance of immediate weight bearing are key to success of the operation.

Objecitve To observe the diffusion and distribution of imported Oncomdania snails in non-endemic areas of schistosomiasis.so as to provide scientific basis for developing the scheme of snail surveillance.Methods Oncomelania snails from the msrshland of Dongting Lake were imported in a waste land in March,2008,Qingxin County,which belonged to non-endemic areas of schistosomiasis.and their diffusion and distribution condition were observed from May to December in the same year.Resuits A total of 1 600 Oncomelania snails were imported.The occurrence rate of frame with snails in sunny days and cloudy rainy days were22.88%and 29.24%,respectively,and there was a statistically significant difference between them(χ~2=4.113,P=0.043).Those in waste Land and the sides/bottoms of the ditches were 9.38%and 35.99%,respectively,and the difference between them also showed a statistical significance(χ~2=67.246,P<0.001).The average density of living snails in sunny days and cloudy rainy days were 0.79 and 1.11 No./0.1 m~2,respectively,and those in waste land and the sides/bottoms of the ditches were0.11 and 1.34 No./0.1 m~2.The furthest diffusion distance of snailswas 4.5 m.Conclusions The detective opportunity of imported snails is relatively high in cloudy rainy days,which mainly distribute at the sides/bottoms of ditches and diffuse along the ditches.

OBJECTIVE:To discuss clinical efficacy and safety of leuprolide acetate in the treatment of endometriosis. METH-ODS:66 endometriosis patients were divided into observation group(37 cases)and control group(29 cases)according to random number table method. Observation group was treated with Leuprolide acetate microsphere for injection,3.75 mg/time,on the upper arm,abdomen or buttocks since the first-fifth day of menstrual cycle(administration time was available at hysterectomized patients disposal),and then every 4 weeks;one administration was recognized as a treatment course. Control group was treated with Ethi-nyl estradiol cyproterone tablet orally,1 tablets per day for 3 weeks,one week drug withdrawal,as a treatment course. 2 groups were given 3-5 courses of treatment according to their tolerance or the improvement of clinical symptoms. The improvement of clini-cal symptoms were compared between 2 groups,and the levels of FSH,LH and E2 were compared before and after treatment;the occurrence of ADR and the time of the return of menses were recorded. RESULTS:2 patients in observation group and 1 patient in control group withdrew from the test. After treatment,total effective rate was 97.14% in observation group,which was significant-ly higher than 75.00% in control group,with statistical significance (P<0.05). Before treatment,there was no statistical signifi-cance in the levels of FSH,LH and E2 between 2 groups (P>0.05). After treatment,the levels of FSH,LH and E2 were de-creased significantly,and the observation group was more significant than the control group,with statistical significance (P<0.05). Total incidence of ADR was 20.00% in observation group,compared to control group (21.42%),there was no statistical significance (P>0.05). The time of the return of menses in observation group was (89.75 ± 3.34) d after drug withdrawal,com-pared to control group [(88.46±2.94)d],there was no statistical significance(P>0.05). CONCLUSIONS:Leuprolide acetate can effectively improve clinical symptoms of patients with endometriosis,and reduce the levels of FSH,LH and E2 with slight ADR, and it doesn’t influence the time of the return of menses.

Objective To discuss the selections of surgical treatment for lower cervical spine fracture and dislocation and the treatment outcome.Methods Clinical data of 50 cases of lower cervical spine fracture and dislocation were analyzed retrospectively.There were 37 males and 13 females at mean age of 41 years (range,20-80 years).Forty-four cases underwent anterior fusion,but 4 with ankylosing spondylitis and 2 with extremely unstable cervical spine were treated with combined posterior fusion.Two cases sustaining ankylosing spondylitis and being unable to be operated via the anterior approach due to the maximum neck flexion limits were treated with posterior decompression and fusion.Two cases developed delayed fracture and underwent posterior release prior to the anterior fixation and fusion.Two cases underwent a second surgery with the posterior release,anterior fusion and posterior fusion performed successively.Neurological performance was evaluated using the American Spinal Injury Association (ASIA)scale.Results All the patients were followed up for mean 28 months (range,12-48 months).Implant loosening and redislocation occurred in one ankylosing spondylitis case 2 months after anterior fusion.Other 49 cases achieved bony fusion in 6 months.Neurological function showed no recovery in complete spinal cord injury cases,but improved for average ASIA 1-2 grades for incomplete spinal cord injury cases.Conclusions Anterior surgery provides good reduction and neurological improvement in treatment of lower cervical spine fracture and dislocation.For extremely unstable cases or difficult anterior reduction cases,posterior surgery or combined anterior/posterior surgery should be considered.