Benign or low-grade malignant tumor of bones neighbouring a joint can be treated by segmental resection and the use of an allograft for reconstruction. Fourteen patients have been treated in this way since 1977, with success in 12 cases and failure in 2 because of infection.Allograft was obtained from fresh cadaver and stored in deep-frozen state (-170C). Within 3 weeks to 6 months, the biological vigor of the bone tissue could be retained and its antigenicity destroyed, so that no immunological reaction took place after replacing the joint and the allograft survived.Eleven patients have been followed up from one and one-half years to five years In the 7 cases with giant-cell tumor at the proximal end of tibia, their range of movement has been attained around 90 degrees. Of the two patients with os-teogenic sarcoma, one has been followed up for one and a half years, and the other for three years. There appears no evidence of local recurrence or distant metastasis in both. The affected limbs are all capable of weight-bearing and locoma-tion.

The traditional treatment for primary malignant tumors involving the innominate bone is hemi-pelvictomy.In recent years, under the control of chemotherapy or radiation, partial or complete resection of innominate bone with lower extremities maintained has been reported.Since 1983, five patients in our hospital have undergone resection of hemi-pelves and femoral heads, and have been inserted by titanium-alloy hemi-pelves and total hip prostheses.The operations were uneventful.Among the five patients, the first and the second were diagnosed as condrosar-coma. Before and after operations, the two patients were treated with a large dosage of chemotherapy and had no reactions.During the follow-up for two years and a half. They were pain-free in affected limbs and could walk with canes.The other three patients diagnosed as giant-cell tumors were followed up from half a year to one year, and they all could walk with crutches.Based on the five cases, we think that in some very carefully selected cases, a limb-saving procedure-inserting a prosthesis after performing hemi-pelvictomy-can lead to a good function and maintain the limb length.

OBJECTIVE To study the correlated factors in acquired infection of liver cirrhosis and offer more reliable theory of treatment for clinic. METHODS The liver cirrhosis history of 302 cases was retrospectively analyzed and reviewed. RESULTS From them 43 cases acquired infection during hospitalization, with 61 case-times of infection, the rate of hospital acquired infection was 14.2%, the rate of case-times of infection was 20.20%. Nineteen cases were died and the mortality rate was 44.19%, Among the 259 non-infected patients only 5 cases were died, the mortality rate was 1.93%, the difference was significant (P

This paper discussed the existing problems of Information System ( LIS) from six aspects, inclu-ding:the security and the expansion of the function, protection of patients′privacy, the establishment of emergency response mechanisms, authenticity and accuracy of data and the risks for auto-approval rules, etc.The authors also analysed the necessity of its rectification from the perspective of ethics, proposed improvement programme and suggestions: strengthening the safety management systems and data, ensuring special check the confidentiality of the project, protect the patient′s privacy;setting up emergency mechanism;hospital based on the actual workload and resources to determine the LIS function module; regularly checking the test data, to avoid occupational inju-ries;continuously improving the rigour of automatic approval rules.

Our experiment aims to compare the effects of "energy-containing lactated Ringer's solution"(EL-RS) with those of "lactated Ringer's solution" (LRS) as an initial resuscitation fluid for the treatment of traumatic shock in rabbits. Deep shock animal models were produced in 74 adult rabbits using the method of pincers pressure and Wiggers' hemorrhagic shock method to produce crush injury of the soft tissue on the thigh and comminuted fracture of the femur. Results showed that blood pressure of the experimental group rose more rapidly than control group and the survival time of the former were longer than the later. The lowering of serum lactate and blood pH etc. showed a similar difference. It is suggested that ELRS is more efficient than LRS for the treatment of traumatic shock in rabbits.

Objective:To analyze the changes in T lymphocyte subsets, B lymphocytes and NK cells in children with active tuberculosis (TB) and their clinical significance.Methods:T lymphocyte subsets, B lymphocytes and NK cells in peripheral blood samples of 106 patients with acute TB (TB group) and 106 healthy children (healthy control group) were detected by flow cytometry and compared between different groups.Results:The percentages of CD3 + T, CD4 + T and NK cells as well as the CD4 +/CD8 + T cell ratio were significantly lower in the TB group than in the healthy control group ( Z=-3.783, P=0.000; Z=-5.401, P=0.000; Z=-3.434, P=0.001; Z=-2.014, P=0.044). The percentages of double negative T (DNT) and B cells in the TB group were significantly higher than those in the healthy control group ( Z=2.765, P=0.006; Z=6.880, P=0.000). No significant difference in the percentage of CD8 + T or double positive T (DPT) cells was observed between the two groups ( P>0.05). The expression of peripheral lymphocyte subsets varied in TB children of different age groups (0-<3, 3-<6, 6-<10 and 10-<16 years old). There were significant differences in CD3 + T, DNT and B cells among the four age groups ( H=10.081, P=0.018; H=14.583, P=0.002; H=8.498, P=0.037). The percentage of CD4 + T cells was significantly lower in children with extrapulmonary TB than in those with pulmonary TB ( Z=-3.068, P=0.002). No statistically significant difference in other lymphocyte subsets was found between children with extrapulmonary and pulmonary TB ( P>0.05). Conclusions:Tuberculosis could lead to immune dysfunction in children. Dynamic monitoring of the changes in peripheral lymphocyte subsets in children with TB could be conducive to better assessment of immune status and providing personalized treatment.

Objective:To explore the effect of goal-directed fluid therapy (GDFT) on the gastrointestinal function of patients after laparoscopic radical resection of cervical cancer.Methods:A total of 60 patients who were scheduled for laparoscopic radical resection of cervical cancer in Shanxi Provincial People's Hospital from October 2016 to September 2018 were selected. They were randomly divided into observation group and control group by random number table method, with 30 cases in each group. Patients in the observation group received GDFT, they were connected to the Flotrac/Vigile monitoring system, and the fluid supplementation was guided according to the changes in mean arterial pressure (MAP), stroke volume variability (SVV) and cardiac index, the goal was to maintain MAP≥60 mmHg (1 mmHg = 0.133 kPa), SVV≤13%, and cardiac index 2.5-4.0 L·min -1·m -2. The conventional fluid therapy was applied in the control group, and the liquid's input speed was adjusted according to the changes of MAP and central venous pressure (CVP) which were respectively maintained at 60-110 mmHg and 8-12 cmH 2O (1 cmH 2O = 0.098 kPa). The crystal/colloid input, bleeding volume and urine output were recorded. The first bowel sounds recovery time, exhaust time, postoperative hospitalization time, and the incidence of nausea and vomiting after surgery were recorded. Arterial blood and central venous blood were drawn before anesthesia induction and 12, 24 and 36 hours after operation to determine the concentrations of arterial blood lactate and central venous oxygen saturation (ScvO 2) as well as intestinal type fatty acid binding protein (IFABP). Results:Compared with the control group, the urine output was increased ( t = -7.738, P < 0.01), the crystal input was reduced ( t = -13.439, P < 0.01), the colloid input was increased ( t = -8.360, P < 0.01), the recovery time of first bowel sounds after surgery was shortened ( t = 6.694, P < 0.01), the exhaust time was shortened ( t = -10.326, P < 0.01), and the time of postoperative hospitalization was shortened ( t = -7.377, P < 0.01). The incidence of nausea and vomiting in the observation group were 10.0% (3/30) and 6.7% (2/30), which were lower than 33.3% (10/30) and 26.7% (8/30) in the control group ( χ2 = 4.812, P = 0.028; χ2 = 4.320, P = 0.038). Compared with the control group, the concentration of IFABP in the observation group was reduced at 12 h ( t = 2.983, P = 0.004), 24 h ( t = 6.452, P < 0.01), and 36 h ( t = -3.880, P < 0.01) after surgery; the concentration of lactate in the observation group was reduced at 12 h ( t = -7.377, P < 0.01), 24 h ( t = -6.036, P < 0.01), and 36 h ( t = -8.933, P < 0.01) after surgery; the value of ScvO 2 in the observation group was increased at 12 h ( t = 2.710, P = 0.009) and 24 h ( t = 2.387, P = 0.020) after surgery. Conclusion:GDFT can maintain the balance between oxygen delivery and oxygen consumption in the gastrointestinal mucosa cells, which can promote the recovery of gastrointestinal function of patients undergoing laparoscopic radical resection of cervical cancer.

ObjectiveTo investigate the effects of bone marrow stem cells autologous transplantation after acute myocardial infarction on local mRNA expression of SDF-1,Kit ligand,E-selectin,ICAM-1and VCAM-1.Methods Acute myocardial infarction models were established in 10 swine,which were randomized divided into placebo group (5 swine) and transplantation group (5 swine).After six months,hearts of placebo group and early transplantation group were harvested and mRNA expression of SDF-1,Kit ligand,E-selectin,ICAM-1 and VCAM-1 in infarcted myocardial region was detected.Results The mRNAs of SDF-1,Kit ligand,E-selection,ICAM-1 and VCAM-1 were expressed in the infarcted myocardial region of transplantation group six months after myocardial infarction,but it was not found in the placebo group.ConclusionsBone marrow autologous transplantation after acute myocardial infarction can promote local mRNA expression of SDF-1,Kit ligand,E-selectin,ICAM-1 and VCAM-1,which may be due to the paracrine effects of stem cells.

Objective To explore the possibility of guiding clinical fast diagnosis and rational use of drugs by rapid detection of plasma digoxin concentration(PDC) with i4000.Methods The plasma samples of 132 patients were collected and digoxin concentration was detected by i4000,and the relationship between PDC and clinical effect was analyzed.Then,the regularities of distribution of digoxin concentration also analyzed according to the age and PDC.The patients whose PDC beyond the effective concentration 0.8 ～ 2.0 μg/L,were treated with adjusted dose respectively according to the circumstance and continuous monitoring of PDC.Results In 132 cases,PDC of 106 patients within the therapeutic dose,accounted for 80.30％,and the total effective rate was 86.36％ after treatment.The effective rates in ＜0.8μg/L group,0.8 ～2.0μg/L group and ＞2.0μg/L group were 10.91％,95.28％and 75.00％,respectively.After dosage adjusted for 1 1 cases with PDC ＜ 0.8μg/L and 4 cases ＞ 2.0μg/L,the PDC returned to the effective concentration.The PDC in over 60 years old group was higher than that in 50 ～ 60 years old group.Poisoning symptoms occurred in 7 cases,and symptoms disappeared through adjustment dosages.Conclusion The PDC detection by Abbott i4000 is rapid and easy to operate,and the result is accurate and reliable.

Sciatic neurectomy was performed on the right limb in 30 rats divided into three groups. On the first postoperative days, stainless steel capacitor plates were applied parallel to each other over the midtibial region of the right, neurectomized limb m each animal in the latter two groups, which were stimulated continuously with a 60kHz sinawave signal delivering 5-10V from peak to peak. The first group was served as controls and received no electrical stimulation. At the completion of 12d of electrical stimulation, all rats were sacrificed. A statistically significant enhancement of wet weight, dry weight, ashed weight, ultimate strength and bone density occurred in the stimulated, denervated tibiae of the experimental animals compared with the nonstimulated, denervated tibiae of the control animals. The results show that a sciatic neurectomized osteoporosis was prevented in the tibiae by a capacitively coupled electrical field.