Objective To apply the IQM quality data of the GEM 3000 blood gas analyzer to evaluate the instrument performance and to compared it with the traditional evaluation method .Methods The precision and accuracy were calculated by using the IQM data ,the external quality control substance was adopted to verify the main performance indexes of the blood gas analyzer .Results The precision of PH ,PCO2 and PO2 calculated by using the IQM data was 0 .02% ,2 .16% and 0 .63% ,which by the traditional e-valuation method was 0 .07% ,1 .68% and 1 .28% respectively ,the accuracy of PH ,PCO2 and PO2 calculated by using the IQM data was 0 .00% ,1 .15% and 0 .49% ,which by the traditional evaluation method was 0 .11% ,2 .91% and 1 .07% respectively .Conclu-sion The intelligent quality management can provide objective and reliable indicators for the performance evaluation of the blood gas analyzer ,and compared with the traditional performance evaluation scheme which is more simple and convenient to operate .

Objective To investigate prevalence of resistance genes for β-lactams in clinically isolated multidrug-resistant Acinetobacter baumannii (MDRAB)in a hospital.Methods 22 clinically isolated MDRAB strains were performed antimicrobial susceptibility testing,resistance genes forβ-lactams (TEM,SHV,CTX-M,PER,DHA, IMP ,VIM ,SIM,OXA-23,OXA-24,and OXA-58)in these strains were detected with polymerase chain reaction. Results The resistant rates of 22 isolates of MDRAB to ceftazidime,cefotaxime,cefepime,gentamycin,amikacin, ciprofloxacin,and compound sulfamethoxazole were all 100.00%;to imipenem,meropenem,and cefoperazone/sul-bactam were 50.00%,40.91 %,and 31 .82% respectively,intermediated rates were 31 .82%,36.36%,and 31 .82% respectively.The carriage rates of OXA-23,TEM,IMP ,and PER were 100.00% (n =22),72.73 %(n=16),54.55% (n = 12),and 18.18% (n =4)respectively.Detection results of SHV,CTX-M,DHA,VIM , SIM,OXA-24,and OXA-58 were all negative.Conclusion Carriage of IMP ,TEM,PER,and OXA-23 resistance genes are the major resistance mechanisms of MDRAB to β-lactamase antimicrobial agents in this hospital.

Infantile diarrhea is pediatric clinical common diseases. In this paper, according to the related literature in recent years, the effect mechanism of massage treatment of infantile diarrhea, according to syndrome differentiation treatment based on different massage for parting with Chinese medicine and massage, acupuncture and so on has carried on the induction.

This paper elaborates on the significance of'birth,growth,transformation,harvest,store'from the theory of TCM fi ve elements.Then it mainly discusses the harmonious development of five aspects of TCM science in research,education,market,hospitals and infrastructure from the view of respective functions of'birth,growth,transformation,harvest,store'and the TCM idea that conforming to certain climate can help people keep healthy.Finally,the paper indicates that it is necessary for the government to guide and adjust the development of TCM in different stage from a dynamic view.

Objective To observe the therapeutic effect of porcine myocardium against porcine myocardial infarction (MI) by porcine myocardial infarction model with connexin-43/zonula occludens-1 (CX43/ZO-1) double gene induced pluripotent stem cells (iPS) transplantation.Methods Porcine myocardial infarction model was established by balloon occlusion.One week later,1640 culture medium,CX43 gene ips cells and CX43/ZO-1 gene ips cells were injected into the different coronary arteries.After 3 months,electron microscopy and color Doppler ultrasonography were used to evaluate the curative effect.Results Compared to ips group of CX43 and 1640 culture group,in CX43/ZO-1 gene ips group,electron microscope showed a large number of island-like cardiomyocytes,blood vessels and intact intercalated discs in the infarct area and normal myocardial junction area structure,color Doppler echocardiography showed left ventricular end diastolic diameter reduced,and ejection fraction was significantly increased (P ＜ 0.05).Conclusions Transfection of CX43/ZO-1 double gene ips cells can promote cardiomyocyte regeneration and cardiac function recovery in porcine myocardial infarction model.

This paper discussed the existing problems of Information System ( LIS) from six aspects, inclu-ding:the security and the expansion of the function, protection of patients′privacy, the establishment of emergency response mechanisms, authenticity and accuracy of data and the risks for auto-approval rules, etc.The authors also analysed the necessity of its rectification from the perspective of ethics, proposed improvement programme and suggestions: strengthening the safety management systems and data, ensuring special check the confidentiality of the project, protect the patient′s privacy;setting up emergency mechanism;hospital based on the actual workload and resources to determine the LIS function module; regularly checking the test data, to avoid occupational inju-ries;continuously improving the rigour of automatic approval rules.

Objecitve To observe the diffusion and distribution of imported Oncomdania snails in non-endemic areas of schistosomiasis.so as to provide scientific basis for developing the scheme of snail surveillance.Methods Oncomelania snails from the msrshland of Dongting Lake were imported in a waste land in March,2008,Qingxin County,which belonged to non-endemic areas of schistosomiasis.and their diffusion and distribution condition were observed from May to December in the same year.Resuits A total of 1 600 Oncomelania snails were imported.The occurrence rate of frame with snails in sunny days and cloudy rainy days were22.88%and 29.24%,respectively,and there was a statistically significant difference between them(χ~2=4.113,P=0.043).Those in waste Land and the sides/bottoms of the ditches were 9.38%and 35.99%,respectively,and the difference between them also showed a statistical significance(χ~2=67.246,P<0.001).The average density of living snails in sunny days and cloudy rainy days were 0.79 and 1.11 No./0.1 m~2,respectively,and those in waste land and the sides/bottoms of the ditches were0.11 and 1.34 No./0.1 m~2.The furthest diffusion distance of snailswas 4.5 m.Conclusions The detective opportunity of imported snails is relatively high in cloudy rainy days,which mainly distribute at the sides/bottoms of ditches and diffuse along the ditches.

Objective To identify an suspected Vibrio cholerae isolated from Xiangyang Central Hospital and characterize the strain in terms of antibiotic resistance and relevant virulence genes.Methods Pathogen identification and susceptibility testing were completed with MicroScan WalkAway 40 Automated Microbiology System.Slide agglutination was used for serotyping. PCR and sequencing technology were employed for 16s RNA gene analysis.PCR technique was used to detect six major viru-lence genes.Results This suspectedVibrio cholerae isolate was confirmed as non-O1 and non-O139 Vibrio cholerae .Suscep-tibility testing results indicated that the strain was sensitive to ampicillin,chloramphenicol,trimethoprim-sulfamethoxazole, and tetracycline.16s RNA gene sequence analysis showed 100% homologous with the registered sequence in National Center for Biotechnology Information database.Virulence genes rtxC and toxR were identified.The other virulence genes such as tcpAET,ctxA,hlyA,and tcpACL were negative.Conclusions This suspected Vibrio cholerae isolate is confirmed as non-O1 and non-O139 Vibrio cholerae .The pathogenic factors may be related to the virulence genes rtxC and toxR.

Objective To investigate the clinical significance of 18 F?FDG PET/CT in assessment of Tomita score in spinal metastases. Methods From October 2012 to October 2015, a total of 77 patients (38 males, 39 females;average age:56.5 years) with suspicious spinal metastasis tumors underwent 18F?FDG PET/CT scan and were included in this retrospective study. Spinal metastasis were confirmed by pa?thology. Each patient was evaluated by the scoring system of Tomita according to the grade malignancy of pri?mary lesions and metastasis of organs and bones in PET/CT. Results Seventy?two patients were osteolytic metastases. Among them, only 23 patients received operative procedure. There were 2 patients with osteoblas?tic metastases, and 3 patients with both osteolytic and osteoblastic lesions. The average Tomita score of opera?tion group was 5.7. Two to three scores were assessed in 2 patients, 4-5 scores in 9 patients, 6-7 scores in 9 patients, and 8-10 scores in 3 patients. The average score of non?operation group was 8.8. One case got 2-3 scores, 2 patients got 4-5, 18 patients got 6-7, and 33 patients got 8-10. Conclusion PET/CT is a convenient and effective tool for assessment of Tomita score in patients with spinal metastases.

OBJECTIVE To study the correlated factors in acquired infection of liver cirrhosis and offer more reliable theory of treatment for clinic. METHODS The liver cirrhosis history of 302 cases was retrospectively analyzed and reviewed. RESULTS From them 43 cases acquired infection during hospitalization, with 61 case-times of infection, the rate of hospital acquired infection was 14.2%, the rate of case-times of infection was 20.20%. Nineteen cases were died and the mortality rate was 44.19%, Among the 259 non-infected patients only 5 cases were died, the mortality rate was 1.93%, the difference was significant (P