Objective:To investigate the important relations between Helicobacter pylori (H.pylori) infection and serum gastrin level and nitric oxide synthase (NOS) activity in order to look for a method treating diseases associated with H.pylori.Methods:Before and after triple therapy against H.pylori with omeprazole 20mg twice a day,clarithromycin 0.25,and metronidazole 0.2 3 times a day for two weeks,fifty-nine with active DU and H.pylori positive,and 39 cases with non-ulcer dyspepsia as control were determined for NOS activity of gastric mucosa and fast serum gastrin level.Results:Patients with DU activity and H. pylori infection,before treatment,had an increased in NOS activity of gastric mucosa and fast serum gastrin level than those after treatment and the control,there were great importance( P

The comprehension of the current status and summary of the advantages vs disadvantages of different interventional methods for the treatment of tumor were concluded in the article.Although all these methods have improved the efficiency of tumor therapy,they are still having a lot of space for further development.The author gives a new concept of the molecular interventional therapy of tumor.

Objective To investigate the changes in the expression of nerve growth factor (NGF) and brain-derived neurotrophic (BDNF) in the dorsal root ganglion (DRG) and spinal dorsal horn following peripheral inflammatory hyperalgesia induced by formalin injected into the plantar region of the right hindpaw in rats. Methods Twenty-four adult SD rats weighing 250-400 g were studied. Peripheral inflammatory hyperalgesia was induced by an intraplantar injection of 5% formalin into right hindpaw. The animals were randomly divided into 4 groups (n=6 in each group), group A:control; group B:2 h after formalin injection; group C:24 h after formalin injection and group D: 48 h after formalin injection. Pain response was recorded. The expression of NGF and BDNF in the inflamed skin, DRG and spinal dorsal horn was detected using immuno-histochemistry technique and image analysis method. Results There were significant bi-phasic nociceptive responses induced by intraplantar injection of formalin in rats. The expression of NGF started to increase 2 h after injection, in the inflamed skin, ipsilateral DRG and dorsal horn neurons and reached the peak level at 24 h after injection. The expression of BDNF in ipsilateral DRG and dorsal horn neurons was unchanged 2 h after injection and started to increase at 24 h after injection. There was a positive correlateion between the expression of NGF and BDNF in ipsilateral DRG neurons.Conclusion NGF is not only an important transmitter in the peripheral mechanicsm of inflammatory pain but also involved in central sensitization through facilitating expression of BDNF in dorsal horn neurons.

Objective To study the susceptible drugs for Fusarium , and provide references for clinical treatment. Methods 40 F.solani and 6 F.moniliforme isolates were examined for the susceptibility to 7 antifungal drugs : AmB, ketoconazoie, miconazole, itraconazole, fluconazole , 5 Fc, and terbinafin by broth microdilution of M38 P recommended by NCCLS. Results 40 F.solani :MIC50 AmB 4 ?g/ml, ketoconazoie 16 ?g/ml, fluconazole 64 ?g/ml, itraconazole 16 ?g/ml, 5 Fc 64 ?g/ml, miconazole 16 ?g/ml, terbinafin 0.25 ?g/ml. MIC90: In addition to AmB and terbinafin , others antifungal agents were similar to MIC50, AmB 8 ?g/ml, terbinafin 4 ?g/ml. 6 F.moniliforme: except AmB and fluconazole were similar to MIC50 of 40 F.solani , others were lower than F.solani MIC50. Conclusion The MIC of terbinafin MIC is lower than that of others drugs, it could be used in the treatment of infections coused by Fusarium sp .

Acupuncture Therapy ; Aged ; Hiccup ; therapy ; Humans ; Male

Objective To evaluate the relationship between pharmacodynamics of sufentanil-induced respiratory depression and age factors. Methods Forty ASA Ⅰ or Ⅱ patients scheduled for elective abdominal surgery were randomly divided into 2 groups according to the age: young and middle-aged group (25-64 yr, group M) and elderly group (65-80 yr, group E). EC50 was determined by up-and-down sequential trail. The initial target effect-site concentration (Ce) of sufentanil was set at 0.40 and 0.35 μg/ml in group M and E respectively.Each time Ce decreased/increased by 10% in the next patient depending on whether or not the respiratory depression occurred. Respiratory depression was defined as VT ≤ 5 ml/kg, RR ≤ 8 bpm/min, SpO2 ≤ 94%,PET CO2 ≥ 55 mm Hg, and/or apnea ≥ 15 s. Results The EC50 and 95 % confidence interval of sufentanil causing respiratory depression were 0.61 (0.54-0.70) μg/ml and 0.41 (0.38-0.45) μg/ml in group M and E respectively with the significant difference. Conclusion The efficacy of sufentanil-induced respiratory depression is related to age factors and the elderly patients are more sensitive to sufentanil-induced respiratory depression.

Objective To study the feasibility and indication of twice chemonucleolysis with collagenase for the treatment of lumbar disc herniation.Methods Eighty two patients of lumber disc herniation were treated with twice collagenase chemonucleolysis.All patients were followed up for 3 to 12 months and then the clinical results were assessed according to the Macnab criteria retrospectively.Results Eighty two cases were followed up from 3 to 12 months postoperatively.Fifty one cases were excellent,13 cases good,8 as fair and 10 were poor.The rate of excellent plus good reached 78%,the effective rate was 88%.Conclusion Twice chemonucleolysis with strict indications together with prevention of allergic reaction before,during and after the operation;is safe and effective for treating lumbar disc herniation.(J Intervent Radiol,2007,16:258-259)

Objective To investigate the changes of carotid, lipidemia and fibrinogen in type 2 diabetes mellitus patients complicated with cerebral infarction (DCI). Methods 58 cases of DCI (DCI group) were enrolled in this study, and 36 cases of cerebral infarction without diabetes mellitus served as control group. The location, number and nature of carotid atherosclerotic plaque and inner diameter of carotid artery were assessed by Doppler ultrasonography in all the patients, as well as blood lipid, blood glucose and fibrinogen were evaluated. Results In DCI group, the rate of carotid atherosclerotic plaque was 79.3%, the IMT and inner diameter of common carotid artery in DCI group were significantly different from control group (all (P

Objective To assess the therapeutic effect of oxygen-ozone combined collagenase injection for the treatment of lumbar disc herniation.Methods Oxygen-ozone and collagenase were injected into the lumbar disc or outside the lumbar disc to treat the lumbar disc herniation.After the treatment,the patients were followed-up for 6~12 months,then the results were assessed according to the Macnab criteria.Results Of 183 patients,the effect was excellent in 144,good in 18,fair in 16 and poor in 5.The curative rate was 88.5%,the effective rate was 97.3%.No serious complications occurred in this group.Conclusion Oxygen-ozone combined collagenase injection is very effective,safe,and cheap method for treating lumbar disc herniation.

Objective To review the distribution and shifting trends of cultured bacteria from the aqueous humor and the vitreous body. Methods A retrospective analysis on distribution of Gram′s stain, the distribution and change of isolates was performed in 522 specimens (aqueous humor,261 and vitreous body,261) of patients with suspected endophthalmitis during a 10 year period (1989 1998). Results The positive cultures were 119 (aqueous humor,44 and vitreous body,75) of 522 specimens. The average positive rate was 22.8%. Gram positive cocci constituting 45.4%(54) of total isolates followed by Gram negative bacilli,34.5%(41);Gram positive bacilli, 20.2%(24). In the positive bacterial cultures, enterobacteriaceae was the most common isolate, 18.5%, and the next was micrococcus, 16.0%; coagulase negative staphylococcus,12.6%; and pseudomonas,10.9%.Comparing the data from 1989 through 1993 with the data from 1994 through 1998, the frequency of Gram positive cocci had no significant change, while the frequency of Gram positive bacilli was decreased and the percentage of Gram′s negative bacilli was increased. Conclusions Gram positive cocci and Gram negative bacilli are the predominant pathogens of bacterial endophthalmitis. The percentage of Gram′s negative bacilli has increased for 5 years. It is very important to comprehend the distribution and shifting trends of these pathogenic bacteria for diagnosis, prevention and treatment of bacterial endophthalmitis.