AIM:To investigate the effect of different oxygen concentrations on the differentiation of marrow stroma cells into osteoblasts and to evaluate the expression of Cbf?1 /Runx2,bone-morphogenesis protein 2 (BMP2) and peroxisome proliferator-activated receptor ?2 (PPAR-?2) in bone marrow stromal cells. METHODS:The bone marrow stomal cells obtained from 4-month-old female SD rats were cultured in growth medium and were used between passages 3 to 5. The cells were divided randomly into 4 groups,each group has 8 samples. The cells in all 4 groups were used for the following experiments after cultured with different oxygen concentrations for 3 d in osteoblastic differentiation medium:total cellular RNA was isolated using total RNA kit; RT -PCR was performed to detect the mRNA expression of Cbf?1 /Runx2,BMP2 and PPAR?2. The protein expression of Cbf?1 /Runx2 and BMP2 was assayed by Western blotting. RESULTS:Compared to the cells in normoxia condition (20% ),the mRNA and protein expressions of Runx2 were enhanced significantly,the mRNA expression of BMP2 was also enhanced significantly,the protein expression of BMP2 increased and the mRNA expression of PPAR?2 decreased significantly in the cells cultured with lower oxygen concentrations. The lower oxygen con-centration was in the culture,the more Runx2 mRNA,BMP2 mRNA,BMP2 and Runx2 protein were expressed. On the contrary,hypoxia significantly decreased the expression of PPAR?2 mRNA in bone marrow stronmal cells and the lower the oxy-gen concentration was used,the less expression of PPAR?2 mRNA was achieved. CONCLUSION:Hypoxia promotes the mRNA and protein expressions of Runx2 and BMP2,also significantly decreases the expression of PPAR?2 mRNA in bone marrow stronmal cells in an oxygen concentration dependent manner,indicating that hypoxia significantly stimulates the differentiation of bone marrow stromal cells into osteoblasts instead of lipocytes.

OBJECTIVETo evaluate the clinical feasibility of laparoscope-assisted D2 radical total gastrectomy for gastric cancer.

METHODSFrom March 2011 to March 2013, 216 gastric cancer patients underwent D2 radical total gastrectomy in our hospital, including 118 laparoscopic and 98 open procedures. The intraoperative and postoperative outcomes between the two groups were compared.

RESULTSCompared with open surgery group, laparoscopic group had longer operation time [(253.9 ± 26.1) min vs. (206.2 ± 23.9) min, P<0.01], less intraoperative blood loss [(138.4 ± 34.0) ml vs. (266.3 ± 58.7) ml, P<0.01], shorter postoperative hospital stay [(10.3 ± 1.4) d vs. (13.9 ± 2.0) d, P<0.01], and lower morbidity of postoperative complication [16.1% (19/118) vs. 29.6% (29/98), P<0.05]. There were no significant differences in the number of lymph node removed [(26.2 ± 6.2 vs. 27.6 ± 5.6)], postoperative morbidity of complication [1.7% (2/118) vs. 2.0% (2/98)], and 2-year survival rate(99.0% vs. 98.6%) between the two groups (all P>0.05).

CONCLUSIONAs compared to open surgery, laparoscope-assisted D2 radical total gastrectomy is safe, feasible with the advantage of rapid postoperative recovery.

Aged ; Female ; Gastrectomy ; methods ; Humans ; Laparoscopy ; Laparotomy ; Male ; Middle Aged ; Retrospective Studies ; Stomach Neoplasms ; surgery ; Treatment Outcome

Objective To evaluate the clinical feasibility of laparoscope-assisted D2 radical total gastrectomy for gastric cancer. Methods From March 2011 to March 2013, 216 gastric cancer patients underwent D2 radical total gastrectomy in our hospital, including 118 laparoscopic and 98 open procedures. The intraoperative and postoperative outcomes between the two groups were compared. Results Compared with open surgery group, laparoscopic group had longer operation time [(253.9 ± 26.1) min vs. (206.2±23.9) min, P<0.01], less intraoperative blood loss[(138.4±34.0) ml vs. (266.3± 58.7) ml, P<0.01], shorter postoperative hospital stay [(10.3±1.4) d vs. (13.9±2.0) d, P<0.01], and lower morbidity of postoperative complication[16.1%(19/118) vs. 29.6%(29/98), P<0.05]. There were no significant differences in the number of lymph node removed [(26.2 ±6.2 vs. 27.6 ±5.6)], postoperative morbidity of complication [1.7%(2/118) vs. 2.0%(2/98)], and 2-year survival rate (99.0% vs. 98.6%) between the two groups (all P>0.05). Conclusion As compared to open surgery, laparoscope-assisted D2 radical total gastrectomy is safe, feasible with the advantage of rapid postoperative recovery.

Objective To evaluate the clinical feasibility of laparoscope-assisted D2 radical total gastrectomy for gastric cancer. Methods From March 2011 to March 2013, 216 gastric cancer patients underwent D2 radical total gastrectomy in our hospital, including 118 laparoscopic and 98 open procedures. The intraoperative and postoperative outcomes between the two groups were compared. Results Compared with open surgery group, laparoscopic group had longer operation time [(253.9 ± 26.1) min vs. (206.2±23.9) min, P<0.01], less intraoperative blood loss[(138.4±34.0) ml vs. (266.3± 58.7) ml, P<0.01], shorter postoperative hospital stay [(10.3±1.4) d vs. (13.9±2.0) d, P<0.01], and lower morbidity of postoperative complication[16.1%(19/118) vs. 29.6%(29/98), P<0.05]. There were no significant differences in the number of lymph node removed [(26.2 ±6.2 vs. 27.6 ±5.6)], postoperative morbidity of complication [1.7%(2/118) vs. 2.0%(2/98)], and 2-year survival rate (99.0% vs. 98.6%) between the two groups (all P>0.05). Conclusion As compared to open surgery, laparoscope-assisted D2 radical total gastrectomy is safe, feasible with the advantage of rapid postoperative recovery.