1.Influence of evidence-based nursing on compliance and quality of life of patients with coronary heart disease undergoing percutaneous coronary intervention
Chinese Journal of Practical Nursing 2011;27(22):1-3
Objective To discuss influence of evidence-based nursing on compliance and quality of life of patients with coronary heart disease after percutaneous coronary intervention (PCI). Methods From January to September, 2010, 86 patients with coronary heart disease undergoing percutaneous coronary intervention therapy were randomly divided into the study group and the control group with 43 patients in each group. Two groups of patients underwent percutaneous coronary intervention therapy and routine care after related check-up, and the study group was given comprehensive and systemic evidence-based nursing intervention on the basis of routine care. Medical compliance and life quality and other clinical indicators of the two groups of patients were comparatively analyzed. Results Compared with the control group, fully compliance rate of the study group was significantly higher, and partical compliance rate decreased significantly. At the same time, score of neurological function, psychological function, social function and other aspects of material life were significantly increased in the study group. Conclusions Application of evidence- based nursing has significant meaning for improving compliance and life quality of coronary heart disease patients undergoing percutaneous coronary intervention, which is worthy of further clinical promotion and application.
2.The effect of TFu on the apoptosis and proliferation of FRH-0201 cholangiocarcinoma cell line
Chinese Journal of Current Advances in General Surgery 1999;0(04):-
Objective:To investigate the effect of TFu on FRH-0201 cell and to test new drugs for the chemotherapy of cholangiocarcinoma.Methods:Morphological changes were observed by light microscopy and fluorescence microscopy.The effect of TFu on FRH-0201 cell was examined by clone formation.Cell cycle and apoptosis were examined by flow-cytometric analysis.DNA ladder was used to detect apoptosis.To compare the drug susceptibility of TFu with 5-Fu and several other common drugs by MTT colorimetric assay.Results:TFu significantly inhibited the proliferation and clone formation of FRH-0201 cell line within concentration of 0.016~4mmol/L.The inhibition ratio was dependent on ascending concentration and time,and that is higher than 5-Fu with the same concentration.The morphological character of apoptosis was observed.Flow cytometric analysis indicated that TFu induced a G_1 phase arrest and apoptosis more than 5-Fu.ADM,DDP and TFu were the sensitive drugs to FRH-0201 cel line,and the combination of them can reach the highest inhibition ratio.Conclusion:These results suggests that TFu can more significantly inhibited the proliferation of FRH-0201 cell line than 5-Fu.The mechanism may be related to the cell cycle arrest in association with apoptosis,which may provide a new approach for treating cholangiocarcinoma.
3.Pinggan Yishen Ditan Decoction for Hypertensive Obesity:A Clinical Observation of 31 Cases
Journal of Traditional Chinese Medicine 1993;0(02):-
Objective To observe the therapeutic effect of Pinggan Yishen Ditan Decoction (Liver-Calming Kidney-Boosting Phlegm-Flushing Decoction) for hypertensive obesity and its possible mechanism.Methods Sixty cases of hypertensive obesity were randomly divided into Chinese medicine group (Chinese medicine was added on the basis of treatment in control group) and control group.The treatment course lasted for 8 weeks.Blood pressure (BP),body weight,waist circum-ference(WC),waist-to-hip ratio(WHR),metabolism parameters,fasting insulin(FINS) and Leptin were observed and compared before and after treatment.Results There was significant difference in SBP,DBP,BMI,WC,WHR,FPG,TC,TG,HDL-C,LDL-C,FINS,Leptin and ISI after treatment in Chinese medicine group (P
4.Concentration Determination of m-Nisoldipine in the Plasma of Rhesus Monkeys by RP-HPLC
China Pharmacy 1991;0(02):-
OBJECTIVE: To establish a RP-HPLC method for concentration determination of m-nisoldipine (m-Nis) in the plasma of rhesus monkeys.METHODS: The analysis was performed on a C18 column at 30℃ with a mobile phase of methanol-water (63∶37) at a flow rate of 1.0ml/min.The detection wavelength was 236nm.The sensitivity was 0.05AUFS and the internal standard was nimoldipine.RESULTS: The detectable concentration of m-Nis showed good linearity in the range of 1.75~ 448?g/L.The average recovery of extraction was 84.1%(RSD=4.09%), and the average recovery of method was 98.7%(RSD=2.76%).The processed plasma samples remained stable in quality after having been stored for 7 days, and the plasma remained stable in quality through repeated freeze thawing or stored at low temperature.CONCLUSION: The present method is highly sensitive and reproducible, as well as convenient, which can be used for concentration determination of m-Nis in the plasma of rhesus monkeys.
5.Diagnosis of Low-tension CT and MRI on Carcinoma Around Vater’s Ampulla
Journal of Practical Radiology 2001;0(07):-
Objective To investigate diagnostic value of low-tension computed tomography(CT) and magnetic resonance imaging(MRI) on carcinoma around Vater’s ampulla.Methods The data of CT and MRI of 30 patients with carcinoma around Vater’s ampulla confirmed by pathology were analysed retrospectively.Results 30 patients all had different degree obstruction of low part of biliary tract. CT or MRI showed the location, size and relationship with abjacent tissue of carcinoma in 26 patients, and the finger-stall sign of the descending part of the duodenum in 18 patients among them. Space occupying lesion of carcinoma was found dimly in 4 patients, no space occupying lesion of carcinoma was found in 3 patients.Conclusion The finger-stall sign of the descending part of the duodenum and finding of mass of ampulla are characteristic findings of carcinoma around Vaterys ampulla, low-tension CT and MRI are valuable methods of diagnosis of carcinoma around Vatery’s ampulla.
6.Comparison of Suicide Attempters and Matched Normal Control
Zhiqing WANG ; Anwen WANG ; Yongche XU
Chinese Mental Health Journal 2002;0(12):-
Objective:To compare suicide attempters with community-based controls for more specific prevention strategies.Method:From the list of suicide attempters of three general hospitals 2 years before, 363 of 786 attempters (48.5%) were found. We collected a control group matched for sex, age and location of residence and compared the life events for the past two years, life quality, family cohesion and adaptability of the past month, and impulsivity and aggressiveness.Result:The two groups were similar in sex, age, family income, marital status, occupation and health insurance status. But the suicide attempt group had less education, had more prominent impulsive and aggressive in traits, had higher levels of hopelessness, more negative life events and greater distress from negative life events in the two years after their attempt. They had poorer life quality and lower family cohesion and adaptability for the past month. They had also more severe depressive symptoms in the prior week.Conclusion:Two years after their suicide attempt, the attempters still have substantial differences from normal controls. They are a distinct high-risk subgroup that deserves specific interventions.
7.Construction of cDNA library of human epithelial ovarian carcinoma tissue
Ling WANG ; Zhiqing LIANG ; Guangwu XIONG
Journal of Third Military Medical University 2003;0(22):-
Objective To construct a cDNA library of human epithelial ovarian carcinoma tissue for screening ovarian carcinoma specific-antigen.Methods The total RNA was separated from human epithelial ovarian carcinoma tissue.The mRNA from total RNA was isolated to synthesize the first and second strand cDNA.The ds-cDNA termini were blunted with pfu DNA polymerase.The blunted cDNAs were added EcoR Ⅰ adaptor and then digested by XhoⅠ.Small cDNA molecules(less than 400 bp) were removed through size fraction.After the cDNAs were ligated into ZAP expression vector,the ligated products were packaged in vitro and the bacteriophage particles infected the host strains XL1-Blue MRF′.Results The efficiency of the primary library was 5.5?10~(6)pfu/ml and the amplified library was 3.0?10~(11)pfu/ml with 96% clones positive.The average length of the inserted fragment was over 1 kb.Conclusion The quality of the constructed human epithelial ovarian carcinoma tissue cDNA library is excellent and helpful to screen ovarian carcinoma specific-antigen.
8.Investigation on Epidemiology and Relativity between TCM Types and Influencing Factors to Hypertension Fatness in Zhejiang province
Zikuang WANG ; Zhiqing CHENG ; Xin QI
Journal of Zhejiang Chinese Medical University 2006;0(02):-
[objective]Get to know something about middle-aged and senile hypertension fat people in Zhejiang province,analyse the relativity between TCM types and factors influencing hypertension fatness(HF),offer foundation to the prevention and treatment of HF with TCM. [Method]Take hypertension people over 40 years old as subjects in some communities of urban districts of Hangzhou city,Shaoxing city,Quzhou city and Wenzhou city in Zhejiang province;by filling in the table of“investigation table on epidemiology of community hypertension”,study the disease characteristics,distribution of TCM types of hypertensive fatness and the relativity between factors influencing hypertensive fatness and TCM types.[Result](1)In 3795 hypertension patients investigated,1444 are of hypertensive fatness,occupying 36.33%;among which,785 are overweighted,659 fat,798 central fat,646 peripheral fat,632 class I hypertension,559 class Ⅱ,253 class Ⅲ.(2)On TCM types,most are of accumulation of sputum wetness,occupying 50.07%,25.00%,13.99% and 11.94% for Yin deficiency and overwhelming Yang,deficiency of Yin and Yang and overwhelming liver fire respectively.(3)There’s relationship between ages,education,blood pressure classes and TCM types(P
9.Analysis of Medical Electrical Equipment for General Safety Requirements and the Environmental Requirements Test
Zhiqing ZHANG ; Jiangong WANG ; Zhihong LIU
Chinese Medical Equipment Journal 2004;0(08):-
According to the Common Specification of Medical Electrical Equipment with GB 9706.1—2007 and the Environment Requirements and Testing Ways of Medical Electrical Equipment with GB/T 14710—1993,the solving methods in the electrical examination are put forward. Application of the practical method of operation,reasonable and effective protective measures to address the medical electrical equipment for general safety requirements and the environmental requirements test and the actual operation encountered some problems. The medical electrical equipment has been designed and tested in scientific and accurate electrical testing,electric shock protection,environmental testing,and the results of the operation specification.
10.Isolation, Culture and Characterization of Endothelial Cells from Capillary Lymphantic Vessels of Human Skin
Ling WANG ; Zhiqing LIANG ; Yudi LI
Journal of Practical Obstetrics and Gynecology 2010;26(3):196-198,前插1
Objective:Human capillary lymphatic endothelial cells (LECs) were isolated and cultured to assist further investigation of the function of lymphatic vessels generation during cancer metastasis. Methods:Human skin was digested by type I collagenase. Cells were isolated using magnetic beads which were marked by monoclonal antibodies against the extracellular domain of VEGFR-3, and purified by cloning col-umn. The morphology and structure of cells were observed by microscopy. Immunophenotype was identified by immunofluorescence. Cell growth curve was recorded to measure the effect of VEGF-C protein. Results:LECs exhibited the typical cobblestone morphology as monolayer growth pattem under microscopy. Enlarged nucleus and rich cytoplasm with bubbles were found under electromicroscopy. LECs specific markers inclu-ding VEGFR-3, LYVE-1, Podoplaninand D2-40 all were positive. VIII factor as specific marker of blood ves-sel endothelium cells (BVECs) was negative. VEGF-C induced a marked increase of cell proliferation. Con-clusions:Human dermal LECs could be harvested successfully using collagenase digestion, immunomagnet-ic beads sorting and clonic column purification.