OBJECTIVE:To observe the efficacy and safety of Zhenqi fuzheng granule combined with dasatinib in the treat-ment of BCR/ABL positive leukemia. METHODS:40 patients with BCR-ABL positive chronic myeloid leukemia or PH-positive acute lymphoblastic leukemia were randomly divided into control group(20 cases)and observation group(20 cases). Control group received Dasatinib tablet,CML patients in chronic period received 100 mg,once a day,orally,morning or evening;CML patients in acceleration period and blastic period received 140 mg,once a day,orally,morning or evening,swallow whole,no cut or crushed. Observation group additionally received 15 g of Zhenqi fuzheng granule,twice a day. The treatment course for both groups was 24 months. Hematologic response,cytogenetic response and molecular biological response in 2 groups were observed, fatality rate,mean follow-up time and the incidence of adverse reactions were followed-up. RESULTS:CCR in observation group was significantly higher than control group,mean follow-up time was longer than control group,CCR median time was significant-ly shorter than control group,the incidence of adverse reactions and fatality rate were significantly lower than control group,the differences were statistically significant(P<0.05). There were no significant differences in the cytogenetic response and molecular biological response in 2 groups(P>0.05). CONCLUSIONS:Zhenqi fuzheng granule combined with dasatinib is superior to dasat-inib alone in proving hematologic response and safety in the treatment of BCR-ABL positive leukemia,and similar in cytogenetic response and molecular biological response.

Objective To study the expression of LIVIN and PTEN in human gastric cancer and evaluate their clinical significance. Methods The expressions of LIVIN and PTEN were detected by immunohistochemical method in the tissues of 65 primary gastric cancer and 15 normal gastric mucosae. Results The positive expression rate of LIVIN in gastric cancer tissues was significantly higher than that in normal tissues [73.85%(48165) vs 26.67%(4/15)], and the positive expression rate of PTEN was obviously lower than that in normal tissues [30.77%(20/65) vs 66.67%(10/15)](P < 0.05). The positive expressions of LIVIN and PTEN were closely correlated with tumor depth of gastric cancer, histological differentiation grade, lymph node metastasis and TNM stage. The expressions of LIVIN and PTEN had a significant positive correlation (r = -0.362,P =0.003). Conclusion LIVIN and PTEN may play important roles in carcinogenesis of gastric cancer, which may be considered as reliable markers in clinical diagnosis and the prognosis of gastric cancer.

ObjectiveTo evaluate the surgical management regarding acute duodenal ulcer perforation in adolescence.Methods24 cases underwent simple neoplasty with postoperative oral Losec for 3 months,13 cases did subtotal gastrectomy, 3 cases did vagotomy with partial gastrectomy, 5 cases did neoplasty with super-selective vagotomy.ResultsThere was no mortality, patients were followed up for 24 months. Complication rate in neoplasty group was 8.3%, and in gastrectomy group was 38.1% (P0.05).Weight gaining in neoplasty group is better than in gastrectomy group( P

Objective To improve the diagnosis and treatment of liver trauma. Methods We retrospectively analyzed different therapeutic means on liver trauma including 79 cases with operative treatment and 27 cases with nonoperative treatment. Results In nonoperative treatment group, 25 cases were cured, 2 cases died. In operative treatment group, 73 cases were cured, 6 cases died. Cure rate was 92.5%(98/106), mortality was 7.5%(8/106). 5 cases died of major blood vessels rupture, 3 cases died on multiple organ function failure. Postoperative complications included 3 cases of subphrenic infection, 10 cases of hepatic abscess, 8 cases of pleural hydrops, 7 cases of incisional infection. Conclutions Type I of liver trauma can be treated by nonoperation, type Ⅱ～Ⅳ of blunt liver trauma can be treated by nonoperation on the condition of intensive monitoring. Type Ⅱ～ Ⅵ of liver trauma should be operated on emergently in case of massive intraabdominal bleeding and combined organ injury.

Objective To explore the relationship between the pathologic features of the cancer and postoperative recurrence in patients with early stage gastric carcinoma.Methods 139 patients with early stage gastric carcinoma underwent radical resection in our hospital from 1984 to 1995. All cases were followed up for more than 5 years. ? 2 test was used for statistical analysis.Results 7 cases died of hematogenic metastasis, 3 cases died of lymph node metastasis, and stump carcinoma was found in the other 3 cases. The recurrent rate was significantly higher in submucosal tumors (14.1%) than in mucosal tumors (1.9%), in lymphatic and vascular vessel invasion-positive cases (37.5%) than in negative cases (7.6%), in lymph node positive (31.3%) than in negative (6.5%), in synchronous multiple gastric cancer (33.3%) than in solitary tumors (7.9%)(P

Objective To study the expression of high molecular weight cytokeratin (CK34βE12) in breast carcinoma and the relationship between the high molecular weight cytokeratin expression and patient's prognosis. Methods We detected the high molecular weight cytokeratin expression in 100 patient's of breast cancer tissues and paracancerous breast tissue in 30 cases immunohistochemically. The relationship between the expression of high molecular weight cytokeratin in breast cancer tissues and age, menstrual status, estrogen receptor expression, progesterone receptor expression, histology grading, TNM staging, pathology type, and patient survival was analyzed. Results (1) There was no significant correlation between high molecular weight cytokeratin expression of breast -cancer tissues and age, menstrual status, estrogen receptor expression, progesterone receptor expression (all P>0.05). But the correlation was statistically significant between that expression and histology grading, TNM staging, pathology category (all P<0.05). (2) By univariate analysis, the 5-year disease-free survival rate in patients with negative expression was lower than those with positive expression(P<0.05). By multivariate analysis, the expression of high molecular weight cytokeratin was unfavorably related with tumor free survival. Conclusion The expression of high molecular weight cytokeratin in breast carcinoma tissues was correlated with biologic malignancies of breast cancer, the negative high molecular weight cytokeratin expression of breast cancer tissues predicts poor prognosis.

Benign biliary strictures(BBS) have different etiologies, and the most common causes are strictures secondary to iatrogenic and ischemic injury after hepatobiliary and transplantation surgery. Fibroblast proliferating activity, extracellular matrix overdeposition and scar proliferation are closely related to BBS. The mechanism of development of BBS involves a variety of cells, cytokines and extracellular matrix. In recent years, biological treatment is emerging as an effective option for BBS, but the clinical application is not yet mature and the curative effect needs to be evaluated further in the future.

This article summarizes the investigation on infrasound at home and abroad and introduces such issues about infrasound as the effects of infrasound on cardio-vascular system, nervous system, audition and vision, its application to medical device as well as its safe-threshold. Being inaudible and with a mechanism of bio-resonance, infrasound has a brilliant perspective when applied to biomedicine.

Objective To investigate the relationship between different types of acute ischemic stroke in elderly patients and serum oxidized low-density lipoprotein (OX-LDL),blood sugar,C-reactive protein(CRP) and interleukin 6 (IL-6) levels.Methods 100 patients with acute cerebral infarction (group A) and 30 heathy patients (group B)were investigated.The levels of serum OX-LDL,blood sugar,CRP and IL-6 were detected by automatic biochemical analysis.Results The levels of OX-LDL(627.2 ± 174.7 ) μg/L,blood sugar ( 8.8 ± 1.7 ) mmol/L,CRP ( 107.5 ±19.4)mg/L and IL-6(79.5 ± 12.1 )ng/L in patients of group A were significantly higher than group B[ OX-LDL(506.1 ± 152.4) μg/L,blood sugar(6.9 ± 1.5) mmol/L,CRP(87.2 ± 13.6) mg/L and IL-6(58.5 ± 10.3) ng/L](t=2.74,3.18,2.61,2.91,allP＜0.05).Conclusion Elderly patients with acute cerebral infarction were closely related to the elevated levels of plasma OX-LDL,blood sugar,CRP and IL-6.Increased plasma OX-LDL was a risk factor for the main artery atherosclerosis type,and hyperglycemia was primary factor for the small artery atherosclerosis type of acute cerebral infarction.

Objective To investigate the effect of c-fos antisense oligonucleotide on the phenotypes of vascular smooth muscle cells in venous autograftt. Methods The external jugular veins were grafted into abdominal aortic arteries in 20 Wistar rats which were divided into test group and control group randomly. The anastomosis and transplanted veins were coated with c-fos antisense oligonucleotide glue gel in the test group while the control group were merely coated with glue gel. The autografted veins were removed and measured by means of pathology and immunohistochemistry two weeks later. Meanwhile the conversion status of the vascular smooth muscle cells were observed with electron microscope. Results The myo-endothelial structure was observed clearly in test group while it was obscure in control group; the expression of c-fos、c-myc、PCNA in vascular smooth muscle cells was significantly decreased in test group. Conclusions The c-fos antisense oligonucleotide can influence the phenotypes of the vascular smooth muscle cells in autografted veins and inhibit the cells’ proliferation. All these indicate that it is a prospective genetic prophylactic therapy for the restenosis of autografted veins.