Objective To explore the role of new emergency mode "Green track" in treatment on traffic injuries. Methods The data of 2 947 patients with traffic injuries rescued by prehospital emergency "green track" and inhospital emergency treatment from March 1998 to January 2009 were retrospectively studied in aspects of prehospital and inhospital treatment methods and their results. Results Of all patients, 2 089 patients were treated surgically and 858 patients treated conservatively, in which 2 736 patients (92.8%) were cured but 211 (7.2%) died, with mortality rate of 7.2%. Head, face and limbs were the main injury parts. Of all, 1 874 patients (63.6% ) were admitted into the hospital within one hour after injury. Conclusions The new emergency mode "Green track" , either prehospital or inhospital, can markedly improve success rate of treatment for traffic injuries, increase survival rate and is of importance to be clinically applied.

In order to study the association between lower urinary tract infection and cystitis glandularis (CG), 120 cases of CG were diagnosed by cystoscopic biopsy in the suspicious foci of the bladder. Among them, 72 cases were subjected to bacterial counting culture of urine and microscopic examination of urinary sediment, and 60 cases to fluorescence quantitative polymerase chain reaction (FQ-PCR) assay to detect HPV, CMV and HSV DNA in urine samples. In the 72 cases of CG, the positive rate of bacterial counting culture of urine was 15.3% (11/72), and gray zone rate was 18.1% (13/72). 31.9% (23/72) patients were positive in bacterioscopy of urinary sediment. There was statistically significant difference as compared with the control group (P<0.01). Only 4 of 60 urine samples were positive by FQ-PCR in detection of the three viruses mentioned above with the positive rate being 6.67%. Compared with the control group, there was no significant difference (P>0.05). It was concluded that the genesis of CG was closely correlated with the chronic lower urinary tract infection, especially caused by Esch coli.
Cystitis/etiology ; Cystitis/microbiology ; Cystitis/*pathology ; *Escherichia coli Infections ; Metaplasia ; Urinary Bladder/microbiology ; Urinary Bladder/*pathology ; Urinary Tract Infections/complications ; Urinary Tract Infections/*microbiology ; Urinary Tract Infections/pathology

Objective To investigate the ability of auto-dermis grafts in the prevention of epidural fibrosis and adhesions, and provide reference for clinical application. Methods Five Tibet mini-pigs were used and laminectomy was performed in lumbar 2 and 4 segment. Dura was exposed and covered with auto-genous dermis grafts in lumbar 2 (the experimental group), without grafts in lumbar 4 (the autogeoous blank group). The specimens were observed grossly and histologically at 2, 4, 6, 8 and 10 weeks postoperatively. Epidural scar amount and adhere range was evaluated by modified Robertson scoring method and analyzed by SPSS 13.0. No sear and adhesion, 0 point; small amount scar and light-range adhesion, 1 point; medium sear and midrange adhesion, 2 pionts; bulk scar ang high-range adhesion, 3 pionts. Results All grafted dermis survived and thicken obviously than dermis in body surface (P< 0.05). Hair growth, sebaceous gland cyst and sweat cystogenesis were not observed. A larvaceous dissection abruption plane, little cicatricial tis-sue and adhesions rarefaction between dermis and dura were observed in the experimental group. Massive ei-catricial tissue from erector spinae filled in cavitas epiduralis was observed and dura was separated difficult-ly from cicatricial tissue in the autogeous blank group. Epidural scar amount and adhesion range in the ex-perimental group was significantly lower than the blank group (P< 0.05). Conclusion Dermis is a kind of favourable biomaterial with restraining cicatrisation and isolation barrier. Autogenous dermis grafts has the best effect on preventing peridural cicatrisation and adhesion after laminectomy.

Objective To explore the curative effect of family -based socialization management on patients with hypertension in community .Methods A total of 116 patients with hypertension were randomly divided into ob-servation group and control group , 58 patients in each group .Both groups received conventional drug treatment .The control group was given routine chronic disease management and the observation group implemented social manage -ment(patients, families, doctors).Blood pressure(BP),lifestyle and psychological condition were evaluated , and the serum triglyceride ( TG) , low density lipoprotein ( LDL) and homocysteic acid ( Hcy) were detected after one year . Results Blood pressure of patients was dropped significantly in the two groups , as compared with the control group . The BP of patients in the observation group was declined more obviously , the BP up to standard rates , control rates and treatment compliance were raised , the mode of life and mental status were improved obviously , and the serum contents of TG, LDL and Hcy were decreased significantly (p<0.05).Conclusion The use of hypotensorscombined with fam-ily-based social management can effectively help patients to control their blood pressure , raise treatment compli-ance, establish a healthy life style and improve their mental status .The mode is worth of spreading in community .

Objective To determine the significance and expression of S100A9 and NMP238 in cervical carcinoma with different concurrent chemoradiotherapy sensitivities. Methods Fresh carcinoma tissues were collected from untreated cervical carcinoma patients and preserved at -80 ℃. The tissues were classified into 2 groups:a high sensitivity group (HS) and a low sensitivity group(LS) according to their response to concurrent chemoradiotherapy. Protein was separated by 2-dimensional gel electrophoresis (2-DE). Peptide mass fingerprintings (PMF) were acquired by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and the proteins were identified by data searching in the Mascot-database. Differential expressed proteins were assayed by Western blot and immunohistochemistry.Results Most of the gels were clear and were successfully and reproductively analyzed. Intensity and rate of S100A9 expression were higher in the HS group than in the LS group,and those of NMP238 expression were higher in the LS group than in the HS group. Conclusion S100A9 and NMP238 expression is associated with concurrent chemoradiotherapy sensitivity in cervical carcinoma.

Objective To investigate the proteomics differences between the high-sensitivity(HS) and the low-sensitivity(LS)groups of cervical carcinoma treated by concurrent chemoradiotherapy,and to confirm the sensitivity associated proteins in intermediate stage and advanced cervical carcinoma.Methods Fresh carcinoma tissues were collected from 10 untreated cervical carcinoma patients.According to the response to concurrent chemoradiotherapy,the tissues were classified into HS group and LS group.In the first part of our experiment,protein separation was performed using two-dimensional gel electrophoresis(2-DE)with Amersham 18 am linear pH 3-10 immobilized pH gradient(IPG)strips.The images of the gels were analyzed by PD-quest 7.0 software to find the differentially expressed protein-spots in each group.Then the differentially expressed protein-spots were incised from the gels and digested by trypsin.The peptide mass flngerprintings(PMF)was acquired by matrix assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS).The proteins were identified by data searched in the Mascot-database.Two differentially expressed proteins were assayed by western blot and immunohistochemical methods.Results Most of the gels were clear and successfully analyzed by PD-quest 7.0 software.Most of the protein-spots concentrated on the area of 20-100 KDa(Mw)and pH4-8.The average number of the protein-spots was 781±74 in HS group and 766±52 in LS group.The match rate was 87.6%between the two groups.Eight proteins highly in HS group but lowly expressed in LS group included hemoglobin subunit beta,caspase-14 precursor,calmodulin-like,S100-A9 protein(MRP-14),galectin-7,HSKERC4,keratin 19 and actin.Ten proteins highly in LS group but lowly expression in HS group included anti HBs antibody light-chain Fab,laminB1,WARS protein,flavin reductase,glutamate dehydrogenase 1,nuclear matrix protein 238,retinal dehydrogenase 1,AFl 65172,subunit of replicative DNA polymerase and HSP70.The higher expression of HSP70 in LS group and galectin7 in HS groups were further confirmed by western blot and immunohistochemical method.Conclusions The 2-DE gels images are successfully acquired from high-sensitivity group and low-sensitivity group of intermediate stage and advanced cervical carcinoma tissues treated by concurrent chemoradiotherapy.Some differentially expressed proteins between the two groups can be further confirmed by western blot and immunohistochemical method.

BACKGROUND: The existing electrocardiogram(ECG)measurement strongly depends on medical professionals and inefficient high-intensity,or relies on automatic identification method which is not accurately enough.Thus,this is difficult to meet high-speed testing,accurate results and ease application for common people.OBJECTIVE: To develop a new method that was simple and efficient to apply and very easy to learn.METHODS: Algorithms were programmed and test software was developed by delphi7.0.ECG was drawn on screen.The apex,the starting point and the ending point as well as the J-point of each ECG wave were clicked by mouse or stylus.Then the wave parameters and an initial diagnosis could be quickly obtained by test software.RESULTS AND CONCLUSION: The parameters of ECG waveform such as wave height,wave time,PR interval,ST segment,QT segment,PP/RR time,cardiac electrical axis and so on could be accurately measured,and heart rate,heart rhythm and the deflection of cardiac electrical axis could be diagnosed correctly.The method was simple to learn and easy to imply,and it was also efficient,quick and accurate.Thus,it could greatly improve the efficiency of measurement and analysis for specialists,and could meet application requirements of general medicals and ordinary people.

【Objective】To observe the effect of Buzhong Yiqi Wan(BYW)on thyroid hormones in rat model of spleen-asthenia.【Methods】Thirty-four SD male rats were randomized into 3 groups:normal control group(N=10),spleen-asthenia model group(N=12),BYW group(N=12).Except the normal control group,the rats in other groups were given the decoction of Radix et Rhizoma Rhei(4 g?d-1 for each rat)to induce spleen asthenia,and meanwhile,BYW group reveived BYW(3.9 g?kg-1?d-1).The treatment lasted 20 days.Radioimmunoassay was used to detect serum levels of triiodothyronine(T3),thyroxine(T4),reverse triiodothyronine(rT3)and thyroid stimulating hormone(TSH).Meanwhile,the body weight,spleen and thymus weight as well as their ratio with the body weight were also observed.【Results】Serum T3 and T4 levels,spleen and thymus weight as well as their ratio with the body weight were decreased in the model group as compared with those in the normal control group(P

Physical continuity of bone is damaged following bone defects. The use of exogenous bone morphogenetic protein (BMP) treatment depends on appropriate carrier, which releases exogenous BMP and other growth factor into the bone defect site, inducing new bone formation and achieving therapeutic purposes. There are many carrier materials for BMP, including inorganic material, synthetic polymers, biological material, and composites of these three materials. But all have some deficiencies. So many researchers have optimized them by combined, physical and chemical methods to improve the bone inductive activity. BMP, which is released from carrier or local gene, can repair bone defect effectively, affording a new way for bone defect repairing.

Objective To investigate the MSCT(multi-slice computed tomography) manifestation of different kinds of malignant tumor in Renal Sinus.Methods MSCT data of 31 patients with diferent kinds of malignent tumor in Renal Sinus were analyzed retrospectively.Results In our series,15 cases were renal pelvic carcinoma,and central mass in renal sinus with light or middle ehancement were revealed in their MSCT investigation,as well as pelvic filling defect and hydronephrosis to some extent were found in secretory phase of enhanced MSCT.10 cases were renal cell carcinoma with renal sinus invasion,and their MSCT muti-planar reconstruction showed the mass mainly located in renal parenchymal,with dramatical and heterogenous CT enhancement mostly,besides local oppression & latral destruction of pelvic wall were caused by renal pelvic invasion.2 cases leiomyosaocoma in renal sinus were big and had a sharp edge,after adminisration they demonstrated dramatical and heterogenous CT enhancement.3 cases were lymphnode metastasis located in renal sinus or renal gate,appeared as nodular lesion with CT enhancement,and hydronephrosis could be exsisted if renal pelvic were obstructed.1 case were retroperitoneal lymphoma,MSCT muti-planar recon-struction revealed a large retroperitoneal mass derectly invaded into renal sinus,with light or middle homogenous ehancement.Conclusion MSCT has advantages of high speed scan,excellent discrimination,and aplenty post-process technique,and is of diagonotic value to malignant tumor in renal sinus.