Objective To investigate pathogenic factors,prevention approaches and therapeutic methods of neurosurgical postoperative intracranial infection.Methods A total of 89 cases were selected as our subjects from 1432 cases ho had received neurosurgical operation in the First People's Hospital of Shangqiu from April 2009 to April 2012.The clinical data of 89 cases with intracranial infection were retrospectively analyzed.Chisquare test was selected to analyze the factors which might cause infection.Effect of decision criteria was chosen to evaluate the cure effect.Results The infection rate was related to the approach to the post fossa,operation periods above 4 h,ventricular drainage,long indwelling drainage and cerebrospinal fluid (CSF) leakage (P ＜0.05 or P ＜0.01).However,the infection rate was not related to age,sex and application of antibiotics before the operation (P ＞0.05).As to effects,73 cases (82.02％) were cured,and 7 cases (7.87％) were showed sort of effects,as well as 8 cases(8.99％) were improved a little and 1 case(1.12％) with invalid.Conclusion It is important to adopt appropriate operation method,by decreasing operation time,preventing drainage from pollution carefully in order to prevent and decrease the intracranial infection after craniotomy,prevention CSF leakage and proper treatment could effectively cure intracranial infection.

Objective To investigate the clinical effects of early cranioplasty and ventriculoperitoneal shunt in the treatment of traumatic brain injury.Methods 70 patients with traumatic brain injury were randomly divided into two groups.35 cases in the control group were treated with ventriculoperitoneal shunt,and received cranioplasty postoperative 3-6 months.35 patients in study group received early cranioplasty and ventriculoperitoneal shunt in 2-3 months after treatment.The therapeutic effects of the two groups were observed.Results After GCS,the excellent and good rate of study group was 71.4％,which was significantly higher than 57.2％ of the control group (x2 =7.47,P ＜ 0.05).The good rate of the study group was significantly higher than that of the control group (x2 =8.35,P ＜0.05).The moderate disability rate of the study group was significantly lower than that of the control group (x2 =7.33,P ＜0.05).The incidence rate of complications in the study group was 14.3％,which was significantly lower than 34.3％ in the control group (x2 =7.35,P ＜ 0.05).Conclusion Early cranioplasty and ventriculoperitoneal shunt therapy in the treatment of patients with traumatic brain injury can effectively improve clinical recovery of the patients,and reduce the postoperative complications.

Tuo-Fawas one of three internal treatment rules in traditional Chinese medicine (TCM) surgery. It had been widely used in the treatment of superficial suppurative diseases, visceral suppurative diseases and other diseases. This article was aimed to clarify the action mechanism ofTuo-Fa for carrying forward better services in the clinic. This article reviewed and recapitulated related literatures on experimental researches ofTuo-Fa in recent years. It mainly showed the research status on anti-infection, anti-tumor and anti-gastric ulcer withTuo-Fa and its prescriptions. It summarized the existed shortcomings, which was that experimental researches on the action mechanism ofTuo-Fa was still on the initial stage with a few references and obviously lagged behind clinical applications. However, some achievements were also made. It had initially revealed the action mechanism ofTuo-Fa in treatment of pyogenic infection. It also had research on action mechanism of anti-tumor and anti-gastric ulcer treatment. It developed new field for the clinical practice. Finally, the research on action mechanism ofTuo-Fa and future prospects were expressed.

AIM: To study the protective effect of ci stanche deserticola Y. C Ma. on thymocytes in septic rats. METHODS: Cecum ligation perforation (CLP) was used to induce sep sis. Treatment group was treated with cistanche deserticola Y. C Ma. (1.25 g?kg -1?d -1, ig) for 14 days before CLP. Animals were killed 12 h or 24 h after CLP an d thmocyte s were collected. The ratio of thmocyte apoptosis and mitochondrial membrane pot ential were determined by the flow cytometry. The ATP activity was detected by s pectrophotography. RESULTS: The rate of thmocyte apoptosis significantly increased 12 h after CLP. The ATP activity decreased 24 h after CLP was significant. The extract of desert living cistanche effectively repressed the apoptosis of th ymocytes and maintained the mitochondrial membrane potential. CONCLUSIONS: The extract of cistanche deserticola Y. C Ma. p rotects thymocytes against apoptosis induced by sepsis. Maintaining of mitochond rial membrane potential may be the protective mechanism.

Atopic dermatitis is a common chronic recurrent atopic constitution-associated inflammatory skin disease mainly characterized by eczematous lesions and itching. Its etiology and pathogenesis are still not very clear, and there are many controversies about its origin, definition, clinical phenotypes and diagnostic criteria. This review analyzes and discusses these controversies in order to provide a meaningful reference for the diagnosis and treatment of atopic dermatitis.

Objective To investigate the correlation of clinical stage and treatment plan with prognosis in incidental prostatic carcinoma.Methods From 2004 to 2010,a total of 1076 patients diagnosed as BPH underwent transurethral plasma kinetic enucleation of prostate (TUPKEP) in our hospital,and their clinical data were respectively analyzed.Among them,42 cases were found to have incidental prostatic carcinoma.The correlation of clinical stage and treatment plan with prognosis in incidental prostatic carcinoma were observed.Results Among 1076 BPH patients undergoing TUPKEP operation,42 cases (3.9％) aged 56-88 years were found to have incidental prostatic carcinoma,which all displayed as prostatic adenocarcinoma including 18 cases at T1 a stage and 24 cases at T1b stage.10 cases received endocrinal therapy,14 cases received bilateral orchiectomy,14 cases underwent radical prostectomy and 4 cases were treated with watchful waiting.Patients were followed up for 24 to 96 months.The survival rates were 100.0％ and the rates of progression were 0.0％,20.0％,0.0％ in T1 a patients received watchful waiting,androgen-deprivation therapy and radical prostectomy,respectively.Total survival rate was 100.0％ and total rate of progression was 11.1％ in T1a patients.The survival rates were 71.4％,100.0％ and the rates of progression were 42.9％,0.0％ in T1 b patients received androgen-deprivation therapy and radical prostectomy,respectively.Total survival rate was 83.3 ％ and total rate of progression was 25.0 ％ in T1 b patients.Compared with T1 b patients,the total survival rate was higher (x2=18.19,P＜0.01) and the rate of progression was lower in T1 a patients (x2 =6.52,P＜0.05).Conclusions The survival rate in T1 a patients accepted androgen-deprivation therapy is similar to that in T1 a patients with watchful waiting.Compared with T1a patients,the survival rate is lower but the rate of progression is higher in T1 b patients received active androgen-deprivation therapy.The survival rate is higher in T1 b patients treated with radical prostectomy than in those received other treatments.Watchful waiting is acceptable for T1 a patients.T1 b patients should be treated with radical prostectomy for a better effect.

Objective To study the effect of a selective COX-2 inhibitor Celecoxib on cell proliferation and apoptosis of human coloretal cancer cell line HT-29 to seek an effective and safe drug for colon cancer chemoprevention. Methods Using MTT assay, flow cytometry(FCM), acridine orange and ethidium bromide staining, the effect of celecoxib on the proliferation and apoptosis of HT-29 cells were investigated. Results The growth of HT-29 cells was inhibited by celecoxib in a dose- and time- dependent manners. FCM analysis showed that the treated HT-29 cells had typical Sub-G 1 peak, the apoptotic rate of which was (7 31?2 37)%～(48 3?2 86)%. The cell ratio of G 0/G 1 phase increased, whereas the cell ratio of S and G 2/M phases decreased after treatment, which was in a dose-dependent manner as well. The treated HT-29 cells exhibited some morphological features of apoptosis, including cell shrinkage, nuclear condensation, DNA fragmentation, and the formation of apoptosis bodies, the apoptotic index of which was in a dose- and time- dependent manners. Conclusions Celecoxib inhibited proliferation and induced apoptosis of human colorectal cancer cell line HT-29, which may be related to blocking the cell cycle progress of HT-29 cells.

OBJECTIVE:To determine the contents of fleroxacin and tinidazole in compound fleroxacin suppository METH_ODS:RP-HPLC was used,the detecting conditions:ODS-C18 column;mobile phase,0 05mol/L lemon acid-acetonitrile(65∶35),was modulated to pH=4 0 with triethylamine;flow rate 0 8ml/min;external standard method;detective wavelength 300nm RESULTS:Linear ranges were 9 92～79 36?g/ml(for fleroxacin) 20 32～162 56?g/ml(for tinidazole) respectively wh_ere the peak areas were correlative with the concentrations,r=0 9 974 and 0 9 995 The average recoveries were 101 6% and 100 1% and RSDs was 1 4% and 1 2% respectively CONCLUSION:This detecting method is simple,rapid,accurate and suitable for determination of the contents of this preparation

Objective To investigate the residual functions in the hepatic lobe with deprivation of bile duct by observation of histomorphological and functional changes in the hepatic cells in a rat model. Methods The right hepatic bile ducts of rats were obstructed with cyanoacrylate and then ligated two weeks after the left and caudate lobes were removed. The lobus quadratus hepatis served as self-control with or without portal vein ligation. The venous blood from individualized liver lobe was examined and histological observation was done to examine the histomorphological and functional changes in the hepatic cells. Results Compared to the controls of both sham operation and untreated lobe, the hepatic lobe with bile ducts deprived did not undergo atrophy significantly with few ultrastructural changes. The hepatocytes of the hepatic lobe with bile duct deprived contained plenty of mitochondria, ribosomes and rough endoplasmic reticulum when observed under transmission electron microscope (TEM). The results of periodic acid-Schiff staining revealed normal glycogen metabolism in the hepatic cells. No significant decrease was detected in the serum albumin and fibrinogen concentrations by examination of blood collected from vein of this liver lobe with deprivation of bile ducts. Conclusions The liver lobes with bile ducts deprived retain most of the liver functions such as protein synthesis and nutrition metabolism, and show no atrophy during the observation period. It is suggested that the hepatic lobes with bile duct deprived still possess the value of preservation. The present study has founded a theoretic basis for remaining the remaining liver lobes with bile duct deprived or ligated in some emergency and specific clinical situations.

Objective To investigate the effect of indomethacin on the protein expression profile of colorectal cancer cell line HCT116 xenografts in nude mice, and to screen the indomethacin anticancer-related proteins. Methods The nude mice with colorectal cancer cell line HCT116 xenograft were treated with or without indomethacin, and then the total proteins extracted from xenografts were separated and visualized by immobilized pH gradient two-dimensional gel (2-DE) electrophoresis. The differential expression proteins between the experimental and control groups were analyzed using PDQuest image analysis software, and identified by peptide mass fingerprinting based on matrix-assisted laser desorption ionization time of flight mass spectrometry and database searching. Results The 2-DE patterns with high resolution and reproducibility were obtained, displayed about 1100 protein spots in each gel, and the matching rate in the two groups was 96. 0% and 93. 6% , respectively. Thirty-one differentially expressed protein spots were found between the two groups, of which 25 spots decreased and 6 increased in the expression levels in the experimental group than in the control group. Eighteen peptide mass fingerprinting maps were obtained, and twelve proteins including galectin-1, annexin and trancription factor, were primarily identified after database searching, most of which were involved in tumor cell proliferation, invasion, apoptosis and immunity. Conclusions Indomethacin exerts its inhibiting effects on colorectal cancer through regulating multiple proteins expression directly or indirectly. The data will be helpful to elucidate the mechanism inhibiting cancer by indomethacin, and to find new targets of chemotherapeutic drugs.