This paper describes virtual instrumentation and its most representative developing environment-LabVIEW. The use of LabVIEW to program custom functions ,such as signal acquisition ,processing ,system control ,in biomedical research ,is also introduced.

Objective To study the apoptosis of breast cancer cells induced by green tea and the preventive effect of green tea on cancer.Methods Catecholamine,the main components of green tea was added into human breast cancer cell line(MDA-MB-231) with different concentrations,and then human breast cancer cell line was measured by MTT assay,comet assay,flow cytometry and caspase-3 activity assay respectively.Results After treatment with 0.2mmol/L EGCG for 48 h,the cell proliferation was inhibited in the experimental group in MTT assay[minimum absorbance value (0.391±0.041),t=4.223 P<0.01].In comet assay,cells treated with 0.2mmol/L EGCG for 48 h in the experimental group showed a fairly long tail[control group average value (4.92±0.64)μm,the experimental group average value (18.76±1.37)μm,P=0.003].The rate of cell apoptosis increased significantly by testing MDA-MB-231 exposed to EGCG with flow cytometry.The apoptotic rate of the cells exposed to 0.2mmol/L EGCG for 48 h in the experimental group was (29.370±1.485)(t=11.125,P<0.01).EGCG induced apoptosis and caspase-3 activity rate was dependent on time and dose.The OD value of caspase-3 observed by the colorimetric method in cells exposed to 0.2mmol/L EGCG for 48 h in the experimental group was (0.144±0.045)(t=5.321,P<0.01).Conclusion EGCG may play a role in the treatment and prevention of breast cancer by affecting apoptosis.

The placement of stent in coronary artery reduces the restenosis rate of percutaneous transluminal coronary angioplasty(PTCA),but the restenosis has perplexed us so far. May be a novel drug coated stent will bring us a new hope in the coming years.

Objective To investigate change of TLR4 in OLETF (Otsuka Long-Evans Tokushima fatty) rats with in-sulin resistance (IR), and to study the effect of pioglitazone (PIO) on the expression of TLR4, and to explore the possible mechanisms of the PIO reducing the risk of cardiovascular diseases. Methods Twenty four OLETF rats were fed with high-fat diet for 20 weeks to establish the IR model then they were randomly assigned into two groups:the model group (group M), in which the rats were fed with high-fat diet;the PIO group (group P), in which the rats were fed with PIO in addition to high-fat diet . Control group include 12 OLETF rats fed with normal diet (group NC). After 20 weeks of drug intervention, plasma levels of FINS (Fasting INSulin), FBG (Fasting Blood Glucose), blood lipid, IL-18 and TLR4 were assessed in every group. Results Comparing with group NC, FBG, Blood lipid, IL-18 and TLR4 were significantly increased in group M(P＜0.05 or P＜0.01), comparing with group M, FBG, Blood lipid were improved in group P, and serum IL-18, TLR4 were signifi-cantly lower in the group P than that in group M(P＜0.05 or P＜0.01). Conclusion TLR4 may be involved in IR by pro-moting inflammatory response, and PIO can significantly improve IR and inflammatory, and reduce the risk of cardiovascular diseases by inhibiting the expression of TLR4.

BACKGROUND:There are many multimodal analgesia schemes in perioperative period of total knee arthroplasty, but there is no ideal scheme. OBJECTIVE:To explore the effects of multi-mode and preemptive analgesia on analgesic effect after total knee arthroplasty. METHODS:120 patients with severe osteoarthritis who underwent unilateral knee arthroplasty were enroled in this study. According to different analgesic effects, the patients could be divided into four groups (n=30). In the control group, no corresponding analgesic measures were found before and during replacement. In the preemptive analgesia group, celecoxib was oraly taken before replacement. In the cocktail analgesia group, cocktail was periarticularly injected during replacement. In the multimodal combined analgesia group, celecoxib was oraly taken before replacement + cocktail was periarticularly injected during replacement. After replacement, intravenous patient-controled analgesia pump was applied in each group. Active flexion range-of-motion, visual analogue scale score in the resting and active states and knee Keen Society Score were measured at various time points after total knee arthroplasty in four groups. Adverse reactions were recorded after replacement. RESULTS AND CONCLUSION:Active flexion range-of-motion, visual analogue scale score in the resting and active states and knee Keen Society Score were better in the preemptive analgesia, cocktail analgesia and multimodal combined analgesia groups than in the control group at various time points after replacement (alP < 0.05). Above indexes were better in the multimodal combined analgesia group than in the preemptive analgesia and cocktail analgesia groups (alP < 0.05). No significant difference in above indexes was detected between the preemptive analgesia and cocktail analgesia groups (alP > 0.05). No significant difference in the number of cases affecting nausea and vomiting was detected at 2 weeks after replacement in the four groups (P > 0.05). There was no deep venous thrombosis of double lower limbs or necrosis and infection of incision. These findings suggest that the effects of preemptive analgesia before total knee arthroplasty, local injection analgesia during replacement, and the combined analgesia of intravenous patient-controled analgesia pump after replacement were ideal. Adverse reactions did not increase, and the operation was safe. At present, multimodal combined analgesia has been accepted by us, but to achieve truly painless results after total knee arthroplasty stil needs more efforts.

Objective To analyze the relationship between the distribution of visceral adipose and cardiovascular damage in the patients with metabolic syndrome. Methods 108 in-patients were categorized according to the diseases, they were suffering from into metabolic syndrome group (MS, 70 cases), essential hypertension group (EH, 22 cases), and type 2 diabetes mellitus group (T2DM, 16 cases). The areas of both visceral adipose (VA) and subcutaneous adipose (SA) were measured for all the three groups, and then VA/SA was calculated. The relationship of the above variables with left ventricular mass (LVM), LVM-index (LVMI) intima-medial thickness (IMT) of carotid artery, and myocardial ischemia was analysed. Results Compared with T2DM and EH groups, the area with VA was significantly larger in MS group (P

Objective To investigate the relationship between the distribution of abdominal adipose tissue and metabolic abnormality in the patients with metabolic syndrome (MS). Methods 108 patients (MS 70 cases, essential hypertension (EH) 22 cases and type Ⅱ diabetes mellitus (T2DM) 16 cases) were enrolled in this study. The visceral adipose (VA) and subcutaneous adipose (SA) were measured with computerized tomography (CT), the ratio of VA/SA was calculated and the correlation between them was analyzed. Results (1) The VA value in MS group was significantly higher compared to both T2DM and EH groups (P

Objective To optimize the method for total DNA extraction and RAPD analysis of Nervilia fordii (NF),and to study the genetic diversity of different breeds of NF,the substitute of NF and the fake of NF at molecular level.Methods We used low-pH extraction medium with high salt to extract total DNA,and used randomly amplified polymorphic DNAs (RAPD) to select polymorphism primer from 49 random primers.Twenty-two kinds of NF samples were analyzed by RAPD,and classified by SPSS.Genetic diversity were estimated by Shonnon's index and Nei's index.Results A higher puritiy of DNA can be obtained from fresh HF than that from medical materials.We selected 19 polymorphism primers for the cluster analysis of fresh NF and dried medical material.For medical materials of NF,the distance of amplification band of small-leave breed is close to that of middle-size leave breed,but is far away from that of,big-leave breed and Nervilia plicata.For the fresh materials of NF,there breeds of Nervilia fordii can be classified into one kind,the distance of fresh NF band is far from that of Nervilia plicata,Pachyrhizus erosus and the cultured breed,and more far awary from that of Plantago asiatica and Centella asiatica.Shonnon's genetic diversity is 0.463,Nei's genetic diversity is 0.267.Intra-population genetic variation is obvious compared to iner-population genetic variation.The estimated gene flow from Gst (Nm) is 0.94.Conclusion The molecular difference in different breeds of Nervilia Fordii can be used to identify Nervilia Fordii.The genetic diversity of Nervilia fordii is mainly caused by the geography environment.

Objective To investigate the potential factors which have impact on the working space for surgeons in gasless laparoscopic cholecystectomy and to probe into the specifications of gasless surgeries.Methods Clinical data of 49 cases of gasless laparoscopic cholecystectomy between March 2007 and July 2010 were summarized.The impacts of body mass index,bowel preparation and means of anesthesia on operation time and conversion to laparotomy were analyzed.Results For patients with body mass index ≤25and ＞ 25,operation times were (43.0 ± 5.4) min,and (52.8 ± 7.4 ) min,respectively,and rates of conversion to laparotomy were 0/34 and 4/15,respectively.Both indices in the first group were higher.For patients with and without bowel preparation,operation times were (44.5 ± 5.4) min and (46.1 ± 8.0)min,respectively,and rates of conversion to laparotomy were 2/26 and 2/23,respectively.For patients with epidural anesthesia and lumbar/epidural anesthesia,operation times were (46.5 ± 7.9) min and (44.1 ±6.8) min,respectively,and rates of conversion to laparotomy were 2/24 and 2/25,respectively.Conclusions The operation time of gasless laparoscopic cholecystectomy could be affected by many factors,such as body mass index.Gasless operations can be improved by evaluating the conditions of patients correctly and by selecting suitable operation methods,anesthesia means and bowel preparation.

Objective To summery the value of treatment for pancreatic benign tumor following the organ preserve principle.Methods The clinical data of 77 patients with pancreatic benign tumor at the General Hospital of Tianjin Medical University from Jan 2002 to Jan 2012 was analyzed.All patients were divided into traditional pancreatic surgical treatment group (n=24) which include pancreaticduodenectomy(PD) and pancreatic body and tail resection plus spleenectomy(DP) and treatment following organ preserve principle (n =53).Results There are tumor evacuation,segmentectomy,pancreatic head resection with duodenum preserving and pancreatic body and tail resection with spleen preserving in the group of treatment following organ preserve principle.Pathology diagnosis showed insulinoma 30 cases,mucinous cystadenoma 16,serous cystadenoma 10,intraductal papillary mucinous tumor 17,solid pseudopseudopapillary tumor 3 and 1 case of pancreatic intraductal hyperplasia.The rate of pancreatic leakage were 26.3％ (5/19) in traditional surgical treatment group and 24.3％ (9/37) in group of treatment following organ preserving respectively.There was no significant difference between these two groups(P＞0.05).The rate of new development diabetis and aggrasive of the preexist diabetis post the pancreatic surgery were 20.8 ％ (5/24),20 ％ (1/5),13.2(7/53),30 ％ (3/10)respectively.There was no significant difference between these two groups(P=0.485,P=1.000).Conclusions The surgical treatment following the organ preserving is a safe and effective procedure for the patients with pancreatic benign tumor.This new method can preserve the pancreatic parenchymal maximally,avoid the extra-and endo-secrete function loss and preserving the function of spleen.