Lycopene is an important natural carotenoid,and is widely found in human tissues and blood,with the prevention of many types of cancer,anti-aging effect.It can inhibit the proliferation of cancer cells of oral malignancy,and increasing of gap-junction communication between cells to prevent oral cancer,and also can inhibit the development of colorectal cancer by reducing the activity of matrix metalloproteinase.It plays the role of anti-metastasis through enhancing the expression of anti-metastatic gene nm23-H1.

Recently,many studies find that microRNA plays a key role in the development,diagnosis,treatment and prevention of gastric cancer.It is confirmed that miR-451 can be used as a useful biomarker for the screening of gastric cancer.Studies also show that miR-203 and miR-21 may become diagnostic markers of gastric cancer.MicroRNA can play the role of oncogenes,and also can play the role of tumor suppressor gene,such as miR-141 plays the role of tumor suppressor gene through inhibition of the positive fibroblast growth factor receptor 2 gene expression.

The paper explains factors that restricting the development of the mobile healthcare information system in small and medi -um hospitals , summarizes strategies for constructing the mobile healthcare information system in small and medium hospitals , and analy-zes advantages of the Android system as the development platform of the healthcare information system .On the basis of above , it designs a model suitable for the mobile healthcare information system in small and medium hospitals and introduces the system in details including the design principles , structure and service modes for clients , etc.

Objective To design and prepare recombinant mutant human high mobility group box 1 protein (HMGB1s) that can combine with HMGB1 receptors but cannot produce inflammatory effect, and accordingly lead to the creation of a new potential agent for anti-inflammatory therapy. Methods This experiment was based on successful clone and expression of human HMGB1.Six mutant HMGB1 cDNA were designed and constructed by one step inverse PCR. They were cloned into prokaryotic expressive vector pQE80L and followed with production of mutant HMGB1s and identification by Western blotting. Results Six mutant proteins were designed and constructed into prokaryotic expressive vector pQE80L. The recombinant HMGB1 proteins were obtained and identified by Western blotting. Conclusion Human HMGB1 mutants have been successfully constructed and the expression and characterization of intent proteins are identified. It will lay a foundation for further study on biological functions of HMGB1.

Objective To analyze the relationship between the distribution of visceral adipose and cardiovascular damage in the patients with metabolic syndrome. Methods 108 in-patients were categorized according to the diseases, they were suffering from into metabolic syndrome group (MS, 70 cases), essential hypertension group (EH, 22 cases), and type 2 diabetes mellitus group (T2DM, 16 cases). The areas of both visceral adipose (VA) and subcutaneous adipose (SA) were measured for all the three groups, and then VA/SA was calculated. The relationship of the above variables with left ventricular mass (LVM), LVM-index (LVMI) intima-medial thickness (IMT) of carotid artery, and myocardial ischemia was analysed. Results Compared with T2DM and EH groups, the area with VA was significantly larger in MS group (P

Objective To investigate the relationship between the distribution of abdominal adipose tissue and metabolic abnormality in the patients with metabolic syndrome (MS). Methods 108 patients (MS 70 cases, essential hypertension (EH) 22 cases and type Ⅱ diabetes mellitus (T2DM) 16 cases) were enrolled in this study. The visceral adipose (VA) and subcutaneous adipose (SA) were measured with computerized tomography (CT), the ratio of VA/SA was calculated and the correlation between them was analyzed. Results (1) The VA value in MS group was significantly higher compared to both T2DM and EH groups (P

Objective To study the clinical effect of microsurgical treatment via lateral fissure approach on hypertensive basal ganglia hemorrhage.Methods Small bone flap via lateral fissure approach were applied to remove basal ganglia hematoma.Thirty two cases were recruited in the retrospective analysis.Results By head CT scan follow-up 24 h post operation,over 80％ percent of hematoma was successfully removed in 28 cases and over 40％ of hematoma was resided in 4 case.Patients were followed up for 3 - 6 months,according to the ADL (ADL)classification,the outcomes were:Grade Ⅰ in 4 cases( 12.5％ ),grade Ⅱ in 13 cases(40.6％ ),grade Ⅲ in 12 cases (37.5％)and grade Ⅳ in 3 cases (9.4％).No death occurred.Conclusion Small window approach on lateral fissure for microsurgical operation of hypertensive basal ganglia hemorrhage is a less invasive procedure with good surgical exposure and homeostasis.Neurological function was well recovered after surgery.

Objective To investigate the effect of p15~(INK4B)(p15)gene transfection on the proliferation of pancreatic cancer cell line BxPC3.Methods In p15 transfection group.pCDNA3.1(+)p15 was transfected into BxPC3 cell by the vector of Lipofectamine2000.In empty plasmid transfection group, pCDNA3.1(+)neo was transfected into BxPC3 cell with the sa/ne method as a blank control group.In non-transfection group.the BxPC3 cell was not transfected as a negative control group.The p15 mRNA expressions were assayed by RT-PCR,and p15 protein expressions were assayed by Western blot.The proliferation was determined by MTY assay,ultra-structure changes were measured by transmission electron microscope.Cell cycle and apoptosis were measured by flow cytometry.Results In the pCDNA3.1(+)p15 transfeetion group,the expression of p15 mRNA and protein were resumed.Since the 2nd day of culture,the growth of pCDNA3.1(+)p15 transfeetion group was inhibited,till the 7th day,the inhibitory rate was 47.9%,G_0/G_1 Dhase cell accounted for(61.56±3.96)% of all the cells,which was significantly higher than(47.44±6.35)%ofthe black control groups and(49.22±7.23)%0f tlle negative control group(P<0.05).G_1apoptosis peak occurred and the apoptosis rate was(5.27±1.04)%in pCDNA3.1(+)p15 transfection group,which was significantly higher than(0.11±0.06)% of the black control groups and(0.09±0.07)% of the negative control group(P<0.05).Apoptosis was also observed by transmission electron microscope in the pCDNA3.1(+)-p15 transfection group cells.Conclusions After p15 gene transfection,BLPC3 cell proliferation could be significantly inhibited and apoptosis could be induced.

Objective To analyze the clinical characteristics and therapeutic effects of cutaneous metastasis from cholangiocarcinoma. Methods From 2006 to 2009, clinical data of 6 patients found with cutaneous metastasis from cholangiocarcinoma were reported. Perineural and vascular invasion were all found in the primary cholangiocarcinoma in these 6 cases with cutaneous metastasis and results were compared with 60 cases of cholangiocarcinoma without cutaneous metastasis. Follow-up to the 6 patients with cutaneous metastases was conducted at an interval of three months. Results One patient with mixed hepatocellular and cholangiocellular carcinoma developed cutaneous metastatic tumor and the pathological diagnosis was cholangiocellular carcinoma. Three patients with hilar cholangiocarcinoma and two patients with distal cholangiocarcinoma presented cutaneous metastases. All six cases of the primary cancers and cutaneous metastases were diagnosed as moderately- poorly differentiated adenocarcinoma. And there was perineural and vascular invasion in the primary tumors. Perineural and vascular invasion of primary cancer was found in 17of 60 patients with cholangiocarcinoma and without cutaneous metastasis. The cutaneous metastasis was positively correlated to the perineural and vascular invasion in the primary tumor (χ2 = 12. 7288, P ＜0. 001 ). Two of 6 cases presenting solitary nodular cutaneous metastasis received skin tumor excision. These two patients survived 8 and 10 months respectively. Among four patients with multiple cutaneous metastases,two cases received chemotherapy and survived 3 and 6 months respectively, the other two cases refusing treatment survived 3 and 4 months respectively. Conclusions Cutaneous metastasis usually occurred at cholangiocarcinoma with lower differentation and perineural and vascular invasion in the primary tumor.Solitary nodular cutaneous metastasis could be excised, multiple cutaneous metastases had poor prognosis.

Objective To evaluate the radiological signs of synovial chondromatosis and the diagnostic value of radiology.Methods 36 cases with synovial chondromatosis confirmed by histology were retrospectively analyzed.All 36 cases underwent radiography and 13 of them underwent CT examinations,10 of them underwent MRI.Results The knee in 22 cases,hip in 5 cases,ankle in 3 cases,shoulder in 2 cases,elbow in 2 cases,carpal joint in one case and temporomandibular joint in one case were involved in 36 cases.Of them,one joint involved in 31 cases and bilateral knee joints involved in 5 cases.Multiple calcareous loose bodies with different sized(from several diameter to 2.7 cm)were seen inside or surrounding the joints on X-ray and CT images.In 10 cases with MR examination,the calcareous nodules were low signal intensity on both T_1WI and T_2WI in 8 cases.In other 2 cases,the center of calcareous nodules showed as high signal intensity while the rim showed as low signal intensity.Conclusion X-ray,CT and MRI are of significant value in diagnosis of synovial chondromatosis.