Psychological problems interrupt the study and life of overseas students, and even lead to a crisis. Based on a study on the results of questionnaires for undergraduate overseas students' on-campus lives in Chongqing Medical Universities,solitude,frustration and depression,the three most popular psychological problems of overseas students in medical universities are analyzed and countermeasures are put forward.

Objective To understand the professional distribution of different mental illness,to explore the relationship between mental illness and professional distribution for mental illness early prevention,early intervention and rehabilitation therapy.Methods From January 1,2003 to December 31,2007,mental illness patients'clinical data of psychiatric hospital in Taiyuan city area and psychiatric residency in Taiyuan city hospital were retrospectively analyzed.This paper reviewed diagnosis by a psychiatrist unified training according to the international statistical clas-sification of diseases and related health problems(ICD -10)mental and behavior disorders diagnostic point of classifi-cation standards to ensure that the total effective medical record information collected 16 495 cases(group);17 039 114 cases of control group in the same period of Taiyuan and Taiyuan city public security bureau to provide permanent population statistics data.SPSS 13.0 software was used for statistical analysis of the two data.Results Prevalence of mental illness during he occupation of the population of 0.968%,the average age of 38.11 years,and students around the age of 18 retirees,in the 65 years and other occupations in 30 to 40 years old,these were three good age stages of mental illness,the age of the unemployed(including the unemployed,the unemployed and the unemployed)preva-lence was highest,accounting for 4.223%.Workers and farmers followed,business,service providers and students prevalence was lower.In the case group and control group cadres was statistically significant difference in proportion of occupational composition(U =2.67,P <0.01),there was not statistically significant difference in retirees (U =1.14, P >1.14);Workers(U =52.20,P <52.20),farmers(U =15.25,P <15.25 ),unemployed (U =106.16,P <0.001),the case group was significantly higher than the control group,the difference was statistically significant;Pro-fessional and technical personnel(U =13.97,P <0.001 ),business services personnel(U =30.39,P <30.39), student(U =62.94,P <62.94),and other professional(U =23.88,P <0.001)cases constitute a significantly lower than the control group,the difference was statistically significant.Conclusion The mental illness and the profession have certain relevance,different careers or age prevalence and kinds of disease have obvious difference.

Objective Hair follicle stem cells (HFSCs) can be cultured in vti ro to differentiate into chondrocytes, osteoblasts and adipocytes .The aim of the article was to investigate the culture method of HFSCs and their adipogenic and osteogenic differentiation potentials. Methods Microdissection and two-step enzyme digestion were applied to obtain HFSCs , and HFSCs are purified by differ-ential adhesion method.The cells were cultured in keratinocyte serum-free medium obtaining 10% fetal bovine serum.Flow cytometry was used to detect CD34、β1-integrin and CK15.The third-generation HFSCs were cultured by osteogenic inductor and adipogenic induc-tor respectively . Results Cultured HFSCs were homogeneous in shape , bearing the property of great refraction and typical cobble-stone-morph.By flow cytometry, the expression rates of CD34,β1-integrin and CK15 were 59.6%, 97.0%and 61.1%.Stem cells were in good growing condition from the third-generation cell growth curve.21 days after being induced by osteogenic inductor, calcified nodules appeared after alizarin red staining, while 14 days after being induced by adipogenic inductor, intracellular lipid droplets could be observed after oil red O staining. Conclusion Cultured HFSCs are chaterized by strong growth capacity and multiple differentiation potential .

BACKGROUND:The hair fol icle stem cells are usual cultured in Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12 containing 10%fetal bovine serum. Recently, keratinocyte serum-free medium has been used to culture hair fol icle stem cells.
OBJECTIVE:To observe the effects of three different culture media on the proliferation and purity of hair fol icle stem cells.
METHODS:After sterling, the skin of the rat’s beard was cut off, and the vibrissa fol icles were dissected under a stereocroscope. The fol icles were digested by Dispase Ⅱ firstly and by the mixture of trypsin and EDTA secondly. cellsuspension was divided into three pieces based on the average number of cells and respectively cultured by keratinocyte serum-free medium, Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12 containing 10%fetal bovine serum and keratinocyte serum-free medium containing 10%fetal bovine serum. Hair fol icle stem cells were selected by rapid adherence on col agen type Ⅳ, and passaged.
RESULTS AND CONCLUSION:Cultured hair fol icle stem cells spread to the third generation, and trypan blue assay showed there was no difference in cellsurvival rate among the three groups (P>0.05). cellCounting Kit 8 assay showed that the cells grew slowly in the three groups in the first 2 days. At day 4, the hair fol icle stem cells grew into the growth logarithmic phase, and the proliferative activity was ranked as fol ows:keratinocyte serum-free medium+10%fetal bovine serum>Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12+10%fetal bovine serum>keratinocyte serum-free medium with a significant difference between the three groups (P<0.05). Flow cytometric examination showed the expression of CD34 in the keratinocyte serum-free medium+10%fetal bovine serum group was lower than that in the keratinocyte serum free medium group (P<0.05). The expressions of CD34, CK15 andβ1-integrin (CD29) in the Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12+10%fetal bovine serum were lower than those in the other two groups (P<0.05). These findings indicate that we can obtain higher purity hair fol icle stem cells in the keratinocyte serum-free medium+10%fetal bovine serum than in the Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12+10%fetal bovine serum.

ObjectiveTo explore a procedure to correct the multiple facial deformities by using the expanded frontal flap combined with the axial flaps.MethodsAccording to the face deformity we used the rectangle 100-350 ml expanders behind the frontal hairline,after finishing the tissue expanding,adopted two or three axial flaps based on the supraoribital,supratrochlear or temporal vessels. ResultsA total of 13 cases were treated with this approach.7 eases were nose and lip defect reconstruction after burn,in which temporal vessel-based flap was used in 2 cases and supraoribital or supratrochlear vessel-based flaps in 5 cases.The other 6 cases were nasal reconstruction combined with the frontal defect correction by using random flaps,including 2 cases of pigmented nevus,1 neurofibroma,and 3 burn scars.All the flaps survived and satisfactory appearance was obtained.Conclusions The expanded frontal flap combined with axial flap based on multiple vessels is a good approach to correct the multiple facial deformities.

Objective To approach the operative effect of dural bilaminar saturation in frontal -temporal skull defect repairs after standard decompressive craniectomy.Methods Fifty-two patients with titanium cranioplas-ty in frontal-temporal skull defect were retrospectively analyzed;27 of them adopted dural bilaminar saturation in the first standard decompressive craniectomy(test group);25 of them adopted dural monostratal saturation in the first operation (control group).The operation time,blood loss,postoperative complications in the two groups were compared. The mean operation time:the test group was (65.00 ±8.15)min and the control group was (83.00 ± 10.25)min,there was statistically significant differences between the two groups(t =7.059,P<0.05);average amount of bleeding:the test group was (55 ±8)mL and the control group was (79 ±12)mL,there was statistically significant differences between the two groups (t=8.541,P<0.05);postoperative complications:adverse reactions were noted in 4 patients of test group (14.8%),5 cases of control group (20%),there was no significant differences between the two groups (χ2 =0.016,P>0.05).Conclusion Skull repairs in second standard decompressive crani-ectomy by dural bilaminar saturation,can be shortened the operation time and lessened amount of bleeding.

Objective To explore the risk factors affecting prognosis of invasive fungal infection .Methods The clinical data of 208 hospitalized patients diagnosed with invasive fungal infection in Sun Yat-sen memorial hospital from 2007 to 2011 were reviewed . The factors affecting prognosis were determined by univariate and logistic regression analysis .Results The fatality rate of adult inpa-tient with invasive fungal infection was 21%.The univariate analysis showed that difference of seven indicators was significant between the survive group and death group:advanced age (over 60 years old), hospitalization time,fasting plasma glucose level, hypoalbumin-emia, using more than two kinds of broad spectrum antibiotic for more than two weeks , kinds of invasive operations , and multiple organ dysfunction syndrome ( MODS) .Binary logistic regression showed that the difference of five indicators was significant between the two groups:advanced age (over 60 years old), hypoalbuminemia, using more than two kinds of broad spectrum antibiotic for more than two weeks, kinds of invasive operations , and multiple organ dysfunction syndrome .Conclusions The indicators such as advanced age (over 60 years old), hypoalbuminemia, the use of more than two kinds of broad spectrum antibiotic for more than two weeks , kinds of invasive operations , and multiple organ dysfunction syndrome might be independent risk factors of death in patients with invasive fungal infection.Early medical intervention should be made in clinical work , in order to reduce fatality rate of patients with invasive fungal in-fection.

Objective To investigate the effect of simulated 400 m He-O2 saturation diving on the pulmonary oxidative-antioxidative system in rats.Methods SD rats were exposed to 4.1 MPa He-O2 mixture for 24 h in an animal chamber. Normal air pressure was used in the control group .The histopathology , contents of malondialdehyde ( MDA) , 8-hydroxy-2′-deoxyguanosine(8-OHdG),and glutathione(GSH) and activities of superoxide dismutase (SOD) and glutathione peroxi-dase( GSH-Px) in pulmonary tissues were determined .Results No difference was found during pathological examination or in MDA, 8-OHdG and SOD activity.GSH and GSH-Px activity were decreased significantly (P<0.05) after hyperbaric exposure.Conclusion Exposure to 4.1 MPa He-O2 for 24 h may promote oxidative stress and reduce antioxidative capacity rather than cause obvious oxidative damage in rat lungs .

Background Mitochondria are the primary sites for ROS production within cells,Tempol(4-Hydroxy 2,2,6,6,tetramethyl piperidine)is a classic compounds targeting ROS scavengers in mitochondria.Objective To investigate the effects of Tempol on aortic function and remodeling in renovascular hypertensive rats.Methods The 2 kindey 1 clip hypertensive model was established in 24 male Wista rats and randomized to untreated hypertensive rats(n=6) or treated with Tempol(1 mmol/L) in drinking water(n=6) for 8 weeks.BP blood plasma angiotensinⅡ(AngⅡ),nitric oxide(NO),8-iso-PGF_(2?) level were determined.Isometric tension change of aortic rings were recorded;RT-PCR were used to measure the expression of NADPH p22 phox mRNA of aorta.Results Hypertensive rats had highter BP,AngⅡ,8-iso-PGF_(2?),media wall,media wall/lumen(W/L)(P

Objective: To analyze the current situation and characteristics of poor vision of students aged 7-18 in 26 ethnic minorities in China, and to provide scientific basis for the vision prevention and treatment measures for minority students. Methods: The 2014 national survey report on student physique and health was used to collect the data of 80 766 primary and secondary school students of poor vision selected from 26 ethnic minorities, The Chi-square test was used to compare and analyze the visual acuity of Han and Chinese students. Results: The rate of poor eyesight among primary and secondary school students of ethnic minorities was 39.17%. The girls were reported with higher rates of visual impairment than boys, and the difference was highly significant(χ2=1 127.28, P<0.01). High school girls (16-18 years old ) had the highest rate of visual impairment, reaching 63.13%. The rate of vision failure was the lowest among boys in the lower grades of primary school (7 to 12 years old), amounting to 20.50%. The detection rate of poor vision in all ethnic groups ranged from 14.68% to 62.94%. Among them, the top five groups with the highest detection rate of poor vision were Tibetan(62.94%), Zhuang(62.39%), Korean(57.92%), Hui(56.48%) and Naxi(51.35%). The five lowest ethnic groups were the aquarium(14.68%), Wa(17.96%), Kyrgyz(18.30%), Li(19.22%) and Hhani(22.30%). The differences among most ethnic groups were highly significant(χ2=7 910.34, P<0.01). Conclusion The vision level of 26 minority students in China is lower than that of the Han. But there are significant ethnic differences. While intervening in Tibet，Zhuang and other key ethnic students, we should pay attention to gender differences and strengthen the prevention and control of vision of students from all ethnic groups.