Lycopene is an important natural carotenoid,and is widely found in human tissues and blood,with the prevention of many types of cancer,anti-aging effect.It can inhibit the proliferation of cancer cells of oral malignancy,and increasing of gap-junction communication between cells to prevent oral cancer,and also can inhibit the development of colorectal cancer by reducing the activity of matrix metalloproteinase.It plays the role of anti-metastasis through enhancing the expression of anti-metastatic gene nm23-H1.

Recently,many studies find that microRNA plays a key role in the development,diagnosis,treatment and prevention of gastric cancer.It is confirmed that miR-451 can be used as a useful biomarker for the screening of gastric cancer.Studies also show that miR-203 and miR-21 may become diagnostic markers of gastric cancer.MicroRNA can play the role of oncogenes,and also can play the role of tumor suppressor gene,such as miR-141 plays the role of tumor suppressor gene through inhibition of the positive fibroblast growth factor receptor 2 gene expression.

In this article, a method to determine the content of liquiritin, ammonium glycyrrhetate, costunolide and dehydrocostus lactone in Mongolia medicine Ga Ri Di-13 pill was established. The chromatographic condition for liquiritin and ammonium glycyrrhetate was listed as the below: with Dimma column (250 mm×4.6 mm, 5μm) as stationary phase; with acetonitrile (A)-0.4%phosphoric acid (B) as mobile phase; gradient elution: 0-10 min (16%-18% A), 10-30 min (18% A), 30-40 min (18%-27% A), 40-85 min (27%-45% A), and 85-86 min (45%-16%A); column temperature was set at 30℃; detection wavelength was 237 nm; and flow velocity was 1 mL·min-1. The chromatographic column condition for costunalide and dehydrocostus lactone was listed as the below: with Dimma column (250 mm×4.6 mm, 5μm) as stationary phase; with acetonitrile-water (65:35) as mobile phase; detection wavelength was 225 nm; column temperature was set at 30℃ and flow velocity was 1.0 mL·min-1. The linearity ranges of liquiritin, ammonium glycyrrhetate, costunolide, and dehydrocostus lactone were 0.1-1.2μg (r=0.999 9), 0.341-4.092μg (r=1.000 0), 0.12-1.2μg (r=1.000 0), and 0.106-1.06 (r=1.000 0), respectively; the average recovery rates were 97.07%, 100.13%, 98.44%, and 98.90%, respectively; the RSD were 1.00%, 1.84%, 2.21% and 3.38%,respectively. This method is specificity and reproducible, and can be used to determination of liquiritin, ammonium glycyrrhetate, costunolide and dehydrocostus lactone in Mongolia medicine Ga Ri Di -13 pill.

After many targeted drugs have entered into the treatment guideline or all stage clinical trial of non-small-cell lung cancer (NSCLC), many researchers undertake deep investigation on the role of molecular gene marker in targeted therapy of NSCLC. It is a good outset that mutation detection of epidermal growth factor receptor (EGFR) gene direct the selection of EFGR tyrosine kinase inhibitors (EGFR-TKI). To choose individualized therapy regimens according to molecular gene marker will be the important research direction, but also be the critical measure to improve the management level of NSCLC and prolong patient"s survival. Accompanied with deep investigation of targeted therapy, there will be more molecular gene markers to direct the formulation of individualized therapy regimens.

Objective To study the effects of high cholesterol diet and activated macrophages (M) on glycoprotein secretion from the gallbladder tissue of guniea pig.Method Forty guniea pigs were randomized into group A fed with ordinary diet and group B fed with a diet containing 1.2% cholesterol for one week.Glycoprotein secretion from guinea pig gallbladder was observed in tissue culture using ~3H-glucesamine as a precursor,and in the meantime,with hydrocortisone and activated M to understand the effects on glycoprotein synthesis and secre- tion function of gallbladder epithelium.Results The activity of peritoneal M was significantly increased in guinea pigs fed with high cholesterol diet.High cholesterol diet induced significant release of ~3H-glucosamine-labeled gly- coprotein into the tissue culture medium as compared with the control level of guinea pig fed with normal diet.The gallbladder tissues were co-cultured for 16 hours with peritoneal M of guinea pig fed with high cholesterol diet. Mucin secretion had an evident increase compared with the controls (with the peritoneal M of guinea pig fed with normal diet at 10~4,10~6 cell/ml).Hydrocortisone (10~(-6),10~(-5),10~(-4)mol/l) caused a reversible dose-dependent inhibition on glycoprotein secretion from the gallbadder tissues of guinea pig fed with high cholesterol diet.Hydro- cortisone (10~(-4)mol/l) also inhibited the stimulatory effect of M activated by high cholesterol diet on glycoprotein hypersecretion in the gallbladder tissues of guinea pig fed with ordinary diet.Conclusion (1) High cholesterol diet can induce the increase of glycoprotein secretion from gallbladder tissues of guinea pig;(2) M can be actvi- ated by high cholesterol diet,which stimulates glycoprotein secretion from the gallbladder tissues of guinea pig. Considering the results of experiment using an animal gallbladder stone model,these findings suggest that the hy- persecretion of glycuprotein from guinea pig gallbladder tissue may be related to guinea pig M activated by high cholesterol diet and stimulated to release TNF,IL-I,etc.

Objective To explore the feasibility of Ilizarov technique in managing large tibia defects combined with soft tissue defects.Methods A total of 24 patients with large tibial defects combined with soft tissue defects caused by compound open tibial fractures were fixed with Ilizarov technique from September 2003 to September 2010.All patients belonged to open tibial fractures,including 20 patients with Gustilo type Ⅲ B and four with Gustilo type Ⅲ C.After debridement,the soft tissue defect areas was 10 cm ×6 cm and the bone defect was(8 ±4)cm.Fifteen patients with tibial defects ＜5 cm were treated with one stage debridement,fibula resection and tibial defect end compression.The other nine patients with tibial defect ＞ 5 cm were managed by one stage debridement,bone transport and bone lengthening.Then,15 patients were treated with one stage debridement,wound closure or wound reduction,bone grafting treatment and second stage cleansing of the incarcerated skin and fracture end.Results All patients were followed up for average 14 months(10-24 months),which showed reconstruction of the bone defects,restoration of the limb length,fracture healing and less than 2 cm difference between health limb and contralateral limb.One patient experienced common peroneal nerve palsy after operation,but recovered three months later.Of all,19 patients recovered without extra surgery,three restored with skin graft and two received skin flap.Conclusion Ilizarov technique is an effective option for treating the tibial defects combined with soft tissue defects at one stage.

Objective To investigate the clinical effects of synthetic medical calcium phosphate and calcium sulfate as the bone replacements for bone defects.MethodsA total of 85 bone trauma patients combined with bone defects treated between February 2000 and October 2011 were randomized to calcium phosphate group ( n =43 ) and calcium sulfate group ( n =42 ) according to bone substitutes.The patients had orthopedic conventional treatment and then received external fixation ( external fixators,plasters) or internal fixation (plates,needles).Forty-three patients in the calcium phosphate group were all treated with medical calcium phosphate and 42 patients in the calcium sulfate group were all treated with medical calcium sulfate.Postoperative complications,fracture healing time and bone substitute absorption time of the two groups were observed and compared.ResultsAfter artificial bone filling for bone defects,all fractures were healed,with average healing time of 12.5 weeks ( range,9-17 weeks) in the calcium phosphate group and 11.3 weeks (range,7-15 weeks) in the calcium sulfate group.No complications or abnormal reactions occurred in the calcium sulfate group,whereas four patients with incision exudation was founded in the calcium phosphate group,and was cured after dressing change.The average follow-up period was 54.5 weeks (range,14-70 weeks).Average absorption time of bone substitute for the calcium phosphate group was 7.3 weeks,longer than that for the calcium sulfate group (P ＜ 0.05 ).Conclusions Medical calcium phosphate and calcium sulfate artificial bones can both be used as bone substitutes and are characterized by convenience,safety and good healing under the auxiliary of internal and external fixators.The calcium sulfate is sturdier and has relatively stronger supporting ability than the calcium phosphate.As the bone substitute,the calcium sulfate has more precise clinical effects,fewer complications and shorter healing time than the calcium phosphate.

Objective To explore the safety and feasibility of operation procedure improvement and process optimization for pancreatoduodenectomy.Methods The effect of the duation time of opertion and postoperative complications of operation procedure improvement and process optimization for pancreatoduodenectomy in 98 patients in the Tumour Hospital Affiliated to Zhengzhou University from August 2008 to May 2015 were retrospective analyzed.Results The duation time of opertion was 145-205 min,average time was 175 min.There were 5 cases (5.1％) of bile leakage,6 case (6.12％) of pancreatic fistula and 4 cases (4.08％) of hemorrhage,and laparotomy revealed that one of them with pancreas-intestinal anastomotic hemorrhage,the haemorrhage stop after sufficient drainage,but due to infection,organ failure,this case was dead half a year after the operation.The remaining patients recovered uneventfully through open drainage,partial flush,somatostatin application,and other conservative treatment.Conclusion The improved pancreatoduodenectomy can shorten operation time and reduce operation complication,is a safe and feasible method,and worthy of clinical popularization.

Objective To design a virtual console for the movable high frequency X-ray machine system type MME2002. Methods An experimental circuit was designed to simulate the real circuit and realized the serial communication between microcomputer and MCU (micro control unit). The simulating software was designed in Visual Basic 6.0, using MSComm to control serial communication. It simulated the functions of the initial console, including setting parameters, sending commands to the machine and storing and transferring the best data of a special condition by file operation in VB. Results The console was designed based on PC. Conclusion This console can replace the console using circuit boards and keys. It can be combined with the X-ray machine systems' image collection software, making the machine's operation more easy and convenient.

Objective To study the preparation and the physicochemical characteristics of glycoprotein from Crassostrea gigas.Methods Oyster collected in Qingdao was taken as the experiment materials,and was extracted with H2O in low temperature.The extracts were purified using Sephacryl s-100 HR gel chromatography and HPLC.Three glycoprotein F22,F33,F42 were finally isolated from the oyster extracts.Results The physicochemical characteristics of glycoprotein were studied.The results showed that the pI value of F22 was 5.5,relative molecular mass of F22 was 34230Da,the protein contents of F22,F33,F42 were 34.1%,19.7% and 12.5%,the saccharide contents of F22,F33,F42 were 35.9%,28.8% and 40%.Conclusion The research result would be used for the study of bioactive component from Crassostrea gigas.