ObjectiveTo investigate the context-sensitive half-times of etomidate. Methods Twenty patients ( ASA Ⅰ - Ⅱ ) underwent selective thoracic operation were divided into 2 groups by random digits table with 10 cases each,the patients were allocated to receive either continuous infusion etomidate 10μg/(kg·min) for 1 h(Gl group) or continuous infusion etomidate 10 μg/(kg·min) for 2 h(G2 group) of maintenance anesthesia. The samples were collected at the following time points: 1 min before stop infusion,2,5, 10,15,20,30,40,50 and 60 min after stop infusion. The plasma concentrations of etomidate were measured by high-performance liquid chromatography and the changes of the context-sensitive half-times of etomidate were calculated. ResultsThe context-sensitive half-times of etomidate was ( 17.1 ± 13.1 )min in G1 group and (21.7 ± 15.1 ) min in G2 group. There was no significant difference between two groups (P＞0.05). ConclusionThe context-sensitive half-times of etomidate is short and its pharmacokinetic profile is suitable for continuous infusion.

The number of IPFI has increased in recent years because of the increasing of immunocompromised hosts due to abuse of antibiotics,glucocorticoids,immunosuppressants and all kinds of invasive operations,organ transplants.The mortality of IPFI remains highly because it's difficult to early diagnosis.Histopathologic biopsy remains as the gold standard for IPFI diagnosis although limited by materials.Imaging manifestations are nonspecific but have important suggestive values to some kinds of fungus.Direct examination is quickly but it's difficult to distinguish strain.Fungal culture can guide the treatment but its positive rate is low and the distinguish for contamination,infection and colonization is hard.Serological test has high sensitivity and specificity,but it cannot distinguish strain and it can yield false positives and false negative.Molecular biology is one of rapid developing methods,but its application is limited due to lack of standardized procedures and standards to assess its results,furthermore it can yield false positives.The proper diagnosis for IPFI relies on the comprehensive assessment combing with advantages and disadvantages of various methods.

Objective　To explore an objective and precise method of measuring the angles of human ears.Methods　Thirty cases (60 ears) were measured by CT.They were performed in position of Frankfort horizontal plane.Results　Auriculo-cranial is 45±14 degrees,cephaloconchal is 83±13 degrees,scaphaconchal is 92±4 degrees,and they are symmetric on both sides.The differences of the angles between men and women are not obvious,except auriculo-cranial angle.Conclusion　The measurement of human auricular angles with CT is a useful and practical method in anthropology and aesthetic medicine.

Bnip3,a kind of mitochondrion apoptosis gene,belongs to the Bcl-2 gene family and BH3-only subfamily.It is one of the downstream response factors of hypoxia inducible factor (HIF).Bnip3 can induce cell apoptosis and autophagy under the oxygen deficit condition.Many studies show that Bnip3 is closely related to the occurrence,development and treatment of tumors.Molecular targeting treatment aimed at Bnip3 combined with chemoradiotherapy has preferable application foreground in tumor therapy.

0 05); At the 7th day, T?RⅠ mRNAs expression was decreased( P

Objective To study the adverse effect of amatoxins on DNA in the histiocytes of rats and the antagonism of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA). Methods SD rats were randomly divided into three groups, the number of males and females was the same. The rats in the experimental groups were respectively treated with amatoxins(0.1 mg/kg bw, i.p.) and amatoxins added EPA, DHA (both were 10 mg/kg bw, i.p.) for 10 days. Single cell gel-electrophoresis was employed to test the damage of DNA in the histiocytes. Results In the group treated with amatoxins, DNA damage in the peripheral lymphocyte, hepatocytes, lung cells ,brain cells was more seriously compared with the control, but no significant DNA damage was seen in the group treated with amatoxins added EPA, DHA. Conclusion Amatoxins may induce DNA damage and eicosapentaenoic acid, docosahexaenoic acid have an antagonism to the adverse effect of amatoxins.

0 05); T?RI mRNA expression was increased at the 1st, 3rd and 7th day, while T?RⅡ mRNA expression was increased at 1st and 3rd , and decreased at the 7th day, compared with normal and sham operation group (P

Objective To investigate the in vitro effect of sirolimus on the apoptosis of a cutaneous squamous cell carcinoma cell line, Colo-16 cells. Methods Cultured Colo-16 cells were treated with different concentrations (50, 100, 150, 200 nmol/L) of sirolimus for various durations ( 12, 24, 48, 72 hours). Subse-quently, cell proliferation was detected by MTT assay, and cell apoptosis by Annexin V-FITC and PI double staining. Morphological changes of the cells were observed with Hoechst 33258 fluorescent staining. Total RNA was extracted from Colo-16 cells treated with sirolimus for 48 hours, and subjected to reverse tran-scription (RT)-PCR for the detection of mRNA expression of B cell lymphoma/leukmia-2 (Bcl-2) and Bcl-2-associated X Protein (Bax). Results Sirolimus inhibited the proliferation of Colo-16 cells in a time-and dose-dependent fashion. The early apoptosis rate was 7.26%±0.26%, 8.34% ±0.19%, 9.86%±0.14%, 11.92% ±0.15% in Colo-16 cells treated with sirolimus of 50, 100, 150, and 200 nmol/L, respectively, signifi-candy higher than that in untreated cells (1.53%±0.09%, P < 0.05); a positive correlation was observed between the apoptosis rate and concentrations of sirolimus (r = 0.955, P = 0.000). Typical morphological changes of apoptosis, such as chromatin condensation and margination as well as nuclear fragmentation were observed by fluorescence staining. After treatment with sirolimus for 48 hours, a significant decrease was observed in the mRNA expression of Bcl-2, while an increase in that of Bax was noticed. Conclusion Sirolimus could induce Colo-16 cells apoptosis in vitro, which may be associated with the modulation of expression of apoptosis-regnlating genes, such as Bcl-2 and Bax.

Objective To investigate the effects of Lycopene ( LP) on immune function of ovarian cancer rats′and its mechanism .Metho ds Sixty ovarian cancer rat models made by injecting ovarian cancer cell line NUTU-19 were divided into five groups randomly:model group,Cisplatin group(2 mg/kg)and LP(40,20,10 mg/kg)group,n =12;the LP and Cisplatin were given by intraperitoneal injection ,once every two days for 5 times.The rats general states and tumor growth conditions were observed ,the tumor tissue morphological changes was observed by H&E staining;the tumor weight ,inhibition rate,spleen lymphocyte transformation rate were de -tected;and the content of IL -2 and TNF-αin serum were detected .The present of CD4 +,CD8 +in blood were detected and the ratio of CD 4 +/CD8 +was calculated .Results The food situation of the rats in LP groups was improved,tumor was reduced,activity and hardness were poorer ,necrosis presented ,tumor cell shrinkage and oth-er pathological morphological changes appeared ,especially the rats in LP 40 mg/kg group .Compared with model group,the tumor weight in LP(40,20 mg/kg)groups was decreased,the inhibition rate and the spleen lymphocyte transformation rate were increased;the contents of IL-2 and TNF-αwere decreased;the CD8+present was de-creased,while the CD4 +present in LP 40 mg/kg group was increased,the ratio of CD4 +/CD8 +was increased. All of the difference above were significant (P<0.05,P<0.01).Conclusion LP can effectively improve the immune function of ovarian cancer rat;which perhaps relates to its effects of reducing the inflammatory cytokines levels and increasing the ratio of CD 4 +/CD8 +.