AIM: The objective of this study was to test the hypothesis that parthenolide suppresses ischemia-induced neuroinflammation in the MCAO model of adult rat. METHODS: MCAO rats were treated i.p. with parthenolide (500 μg/kg). Brain sections were analyzed for BrdU, BrdU-DCX, BrdU-Tuj-1, BrdU-MAP-2 and BrdU-GFAP staining. Total protein was extracted from ischemic striatum, and Western blot was used to determine TNF-α expression. RESULTS: Cerebral ischemia increases expression of TNF-α in the ischemic striatum. Parthenolide suppressed the expression of TNF-α and enhances the proliferation of newborn cells in the ischemic striatum. The cell number of BrdU~+-DCX~+, BrdU~+-Tuj-1~+, and BrdU~+-MAP-2~+ is increased in the ischemic striatum after parthenolide treatment at 3 d, 7 d or 28 d after MCAO. Furthermore, parthenolide depressed the cell number of BrdU~+-GFAP~+ in the ischemic striatum at 3 d, 7 d and 28 d after MCAO. CONCLUSION: Parthenolide inhibits neuroinflammation induced by cerebral ischemia and promotes neurogenesis in the ischemic striatum. Further study of the effects of parthenolide on inflammatory gene expression using model animal systems as described here are critical to elucidating their mechanisms of action.

Fluorescence probe Fluo-3AM can be luminary in confocal microscope after combining Ca2+,So nomadic Ca2+ in cytoplasm can be measured and tested by this way.Some confocal laser scan images are obtained after calcium in muscle fibers signed with F1uo-3AM. Then mapping function is used to convert the gray level image into colorful image by pseudo-color enhancement theory in digital image.Compared to the original gray level image,the processed pseudo-color image could be more easily distinguished.So the methods had a great value in Ca2+ detection fields.

With a PII-300 computer, an intelligentized microcomputer system for HBV marker detection and reporting is developed in this paper, which is based on Windows operating system and accomplished through Visual Basic6.0. More than 10, 000 cases have proved that the result from the system accords with the one from artificial detection completely. This system solves the problems in hospital laboratory such as high labor intensity, low working efficiency and high error rate, and thus a quantitative detection method for HBV marker comes into existence.

With a PII-300 computer, a microcomputer system for SCCA marker detection is developed in this paper, which is based on Windows operating system and Visual Basic 6.0. More than 1,000 cases have proved that the results from the system completely accord with the one from artificial detection. With this system, such disadvantages are conquered as high labor intensity, low working efficiency and high error rate, and a quantitative detection method for SCCA marker comes into existence.

This paper gives the techniques and steps of creating remote control instruments system using Windows API functions,and explains its hardware and software structures.The whole system is proved to be stable and reliable by experiment.It can provide a new experimental method for biomedical research,which enable the construction of multifunctional experiment platform.

Clinical neuroanatomy is a bridge between basic medicine and clinic. To resolve the deficiency existing in PBL teaching,we improved the basic teaching installation and teaching practice and evaluation system. The results indicated that we had gotten satisfactory effect by using teaching method of clinical neuroanatomy

The nonlinear wavelet method is used to detect ECG signal. The ECG signal disrupted by high-frequency noise is decomposed with a dyadic Daubechies wavelet according to Mallat algorithm. The principle of the nonlinear wavelet detection method is discussed. Along with the Lipschitz criterion, it is applied to denoising the disrupted ECG signal. The ECG signal is effectively recovered while denoised.

In allusion to high labor intensity, low working efficiency and high error rate in hospital laboratory, a detection system of the enzyme-linked analyzer based on Multiscan EX instrument is developed. Being easy to operate, the detection system proves effective in enhancing working efficiency. It deserves popularizing because of its comprehensive applications to the detection.

OBJECTIVE: To determine the type and carrier rate of deafness-related variants in Dongguan, China. METHODS: A total of 16 182 subjects were screened. Heel blood samples were collected from newborns, while peripheral venous blood samples were collected from the remainders. For each individual, 100 variations of 18 deafness susceptibility genes were detected. RESULTS: In total 1631 deafness-related variants (including 5 homozygous mutations) were detected, which gave a detection rate of 10.08%. The detection rate of SLC26A4 gene variants was the highest (845 cases, 5.22%), which was followed by GJB2 (673 cases, 4.16%), GJB3 (100 cases, 0.62%), TMC1 (12 cases, 0.07%), and MYO15A (1 case, 0.01%). The detection rate for GJB2 c.235delC variant was the highest (524 cases, 3.24%), which was followed by SLC26A4 IVS7-2A>G variant (270 cases, 1.67%). Thirty three individuals (0.20%) carried two variants at the same time, 7 of them (0.04%) carried compound heterozygous variants of the same gene. CONCLUSION To expand the range of screening can help with determination of the carrier status and provision of early intervention and genetic counseling for the examinees.
China ; DNA Mutational Analysis ; Deafness ; genetics ; Genes ; Genetic Counseling ; Genetic Predisposition to Disease ; Genetic Testing ; Genetic Variation ; Humans ; Infant, Newborn ; Mutation ; RNA, Ribosomal