1.Clinical study on cilostazol combined with pancreatic kininogenase in treatment of LEADDP
Journal of Endocrine Surgery 2011;05(3):197-199,211
Objective To compare clinical effects between cilostazol(CLT)alone and cilostazol combined with pancreatic kininogenase(PK)on lower extremity arterial disease in diabetic patient(LEADDP).Methods Patients with LEADDP were randomly divided into 2 groups:single medication group or the control group(53 cases)and combination group or the treatment group(53 cases).Sugar-reducing medicines were given before and after treatment to keep a steady blood glucose level.The control group took CLT orally 100 mg each time,twice a day.Besides receiving the same dose of CLT,the treatment group took 120 tablet of PK on an empty stomach,three times a day,with the duration of 3 months.Clinical symptoms,ankle/brachial index(ABI),blood rheology,nailfold microcirculation,intimal medial thickness(IMT)and plaque thickness were compared between the 2 groups after treatment.Results Symptoms in both groups improved after treatment.ABI was elevated significantly compared to that before treatment(P<0.05)and the change was more obvious in combination group.The difference of ABI after treatment between the 2 groups had statistical significance(P<0.05).IMT of the right foot dorsal artery,and left and right posterior tibial artery increased significantly compared to that before treatment(P<0.05).Plaque thickness decreased compared to that before treatment(P<0.01)and the difference between before and after treatment in combination group was significantly greater than that in single medication group(P<0.05).Conclusion Cilostazol combined with pancreatic kininogenase has better clinical effects than cilostazol alone in treatment of patients with LEADDP.
2.Clinical effect of intra-articular injection of sodium hyaluronate combined with rehabilitation training in treatment of knee osteoarthritis in the elderly
Chinese Journal of Geriatrics 2011;30(5):384-386
Objective To observe the clinical effect of intra-articular injection of sodium hyaluronate combined with rehabilitation training in treatment of knee osteoarthritis, and their effects on β endorphin and substance P levels in plasma. Methods All 106 cases with knee osteoarthritis were divided into treatment group and control group. Treatment group were treated by intra-articular injection of sodium hyaluronate combined with rehabilitation training, and control group underwent rehabilitation training only. Knee joint function was evaluated using Lysholm scores before and six weeks after treatment, and at 3-month and 6-month follow-up. Meanwhile, the concentrations of β endorphin and substance P in plasma were tested in the two groups. Results Compared with pre- treatment, the Lysholm scores were obviously increased after 6-week treatment, at 3-month and 6-month follow-up in both groups, with more obvious increase in treatment versus control group (P<0.01). The Lysholm scores were higher in treatment group than in control group (P<0.01). The concentration of β endorphin in plasma was significantly increased, and the substance P concentration in plasma was decreased after treatment in both groups. There were also significant differences in concentrations of β endorphin and substance P in plasma between treatment group and control group (P<0.01). Conclusions Intra-articular injection of sodium hyaluronate combined with rehabilitation training is effective in improving knee joint function in elderly patients, in relieving pain and in enhancing joint movement.
3.Role of Serum Creatinine,Cystatin C,Abbreviated MDRD and Le Bricon Formula in the Evaluation of Renal Function after Renal Transplantation
Journal of Modern Laboratory Medicine 2014;(5):97-99,103
Objective To investigate diagnostic value of the serum creatinine (Scr),Cystatin C (Cys C),MDRD and Le Bricon formula in the renal function after renal transplantation.Methods The total renal glomerular filtration rate (GFR)of 5 7 ca-ses of renal transplantation patients and 48 healthy control group were determined using 99mTc-DTPA from 2012 September to 2014 June.Scr,Cys C and calculate the estimated glomerular filtration rate of MDRD and Le Bricon,correlation analysis by Spearman,and use receiver operating characteristic curve (ROC)to evaluate the diagnosis value.Results DTPA,Scr, Cys C,MDRD and Le Bricon intransplantation group was 60.69±13.52 ml/min/1.73m2,1.34±0.28 mg/dl,1.42±0.15 mg/dl,66.97±15.85 ml/min/1.73m2 and 59.93±10.23 ml/min/1.73 m2 ,respectively.Control group were 90.12±12.61 ml/min/1.73m2 ,0.97±0.09 mg/dl,0.86±0.2 mg/dl,87.13±18.29 ml/min/1.73m2 and 94.80±18.73 ml/min/1.73m2 , respectively.There were statistically significant (t=0.237~1.35,P< 0.01)among groups.Scr,Cys C,MDRD and Le Bri-con between DTPA were different correlation (r=-0.76~0.82,P<0.01).The area under ROC curve were 0.856,0.973, 0.814 and 0.971,respectively.Conclusion Scr,Cys C,MDRD,Le Bricon have some predictive value to chronic kidney dis-ease in all patients after renal transplant recipients,but Cys C and Le Bricon were superior to the Scr and MDRD equation.
4.Expression of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in Thyroid Carcinoma Tissue and Their Concentrations in Serum
Chinese Journal of Bases and Clinics in General Surgery 2003;0(04):-
Objective To explore the protein expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1), and to investigate their relationships between their serum concentration before operation and the infiltration and metastasis of thyroid carcinoma. Methods The protein expressions of MMP-9 and TIMP-1 in 32 cases of thyroid carcinomas, 23 cases of adjacent tissues and 30 cases of benign hyperplastic lesions were measured by using immunohistochemistry. The preoperative serum concentrations of MMP-9 and TIMP-1 in 21 cases of thyroid carcinomas and 19 cases of benign hyperplastic lesions were determined by enzyme-linked immunosorbent assay. Results The positive expression rates of MMP-9 and TIMP-1 in tumor tissues were significantly higher (75.0%,56.3%)than those in adjacent tissues and benign hyperplastic lesions (30.4%, 21.7%; 26.7%, 23.3%) P
5.Aplication effect of fiber bronchoscope examination in the treatment of respiratory internal medicine emer-gency and its effect on the quality of life of patients
Chinese Journal of Primary Medicine and Pharmacy 2015;(13):2025-2027
Objective To study the aplication effect of fiber bronchoscope examination in the treatment of respiratory internal medicine emergency and its effect on the quality of life of patients.Methods 156 patients with emergency were randomly divided into the observation group and control group,each group in 78 cases.The control group was given routine treatment plan according to the primary disease type which was the respiratory treatment of the corresponding diseases,the observation group in the control group was added fiberoptic bronchoscopy based on the treatment.Compared blood clinical curative effect,heart rate and quality of life score changes of the two groups of patients after treatment.Results The total effective rate of the observation group was 94.87%,which was significantly higher than 81.01% of the control group(χ2 =6.28,P <0.05).The pH,PaO2 and SaO2 in the observation group after treatment were(7.35 ±0.04),(73.23 ±9.11)mmHg and(92.33 ±4.32)%,which were significantly higher than the corresponding index in the control group(t =3.97,6.08,4.96,all P <0.05);while PaCO2 and HR were (51.53 ±9.24)mmHg,(91.69 ±12.42)times/min,which were significantly lower than the corresponding index in the control group(t =6.73,5.13,all P <0.05);The quality of life score in the observation group was(54.04 ± 10.94)points,which was significantly lower than(77.23 ±12.32)points of the control group(t =5.86,P <0.05). Conclusion Fiberoptic bronchoscopy for respiratory internal medicine emergency treatment can significantly improve the clinical curative effect of treating disease and patient quality of life.
6.MAGI1 inhibits cancer cell migration and invasion of hepatocellular carcinoma via regulating PTEN
Journal of Central South University(Medical Sciences) 2011;36(5):381-385
Objective To explore the biological function and molecular mechanism of membrane associated guanylate kinase,WW and PDZ domain containing 1 (MAGI1) in hepatocellular carcinoma.Methods HepG2MAGI1 stable cell line was constructed by transfecting HepG2 cells with pcDNA3.1-MAGI1 plasmid.Wound healing and invasion assay were performed to compare the migration and invasion ability of HepG2MAGI1 and HepG2 cells.Furthermore,the expression of MAGI1 and phosphatase and tensin homolog deleted on chromosome ten (PTEN) was also examined by Western blot and the relationship was analyzed.Results The wound healing assay showed that the closure of HepG2MAGI1 cells was significantly slower than that of HepG2 cells [(90±10)% vs.(50±15)%,P<0.05],and the invasion assay showed that the number of HepG2MAGI1 cells that passed through the matrigel was fewer than HepG2 cells (68±18 vs.150±30,P<0.05).The protein expression level of PTEN was significantly elevated in HepG2MAGI1 cells compared with HepG2 cells (1.40±0.32 vs.0.28±0.15,P<0.05).MAGI1 and PTEN protein expression levels were positively correlated (r=0.913,P<0.01).Conclusion MAGI1 may inhibit the cancer cell migration and invasion of hepatocellular carcinoma via regulating PTEN.
7.Observation of thyrohyoid muscle change after cutting the recurrent laryngeal nerve in dog
Chinese Archives of Otolaryngology-Head and Neck Surgery 2001;8(1):48-51
Objective.To observation the change of thyrohyoid muscle(THM)after recurrent laryngeal nerve(RLN)has been cutting off.The animal experimental results were reported.Method.The animal experimental model of RLN paralytic was completed in 9 dogs.The specimens of THM were treated by histochemiscal staining after 2,4,6,8,10,20 day operation respectively and the diameter and areas of three types muscle fibers were determined by microscope.Results.The diameters and areas about 3 types muscle fibers of THM descended after operation,the value of them reached the minimum simutaneously,then recovered step by step.Conclusion.The changes of THM was related to operative trauma and nutrition disorder probably,while it was not related to RLN cutting.
8.Effects of perioperative hypothermia on patients’ vital signs during TURP
Zhiming BAI ; Zhenxiang LIU ; Chong ZHANG
Chinese Journal of Urology 2001;0(03):-
Objective To evaluate the effects of unwarmed and warmed irrigation fluid on patients’ vital signs during transurethral resection of the prostate (TURP),and to investigate the related factors affecting the safety of TURP. Methods A total of 100 patients were randomly assigned to receive unwarmed (21℃,n=40)or warmed (37 ℃,n=60)irrigation fluid during TURP.Pre-operatively,their age,body weight,IPSS,ratios of heart, brain, lung complications,intra-operative anesthesia modes,irrigation time, irrigation fluid amount,resected prostate weight and blood transfusion amount were not significantly different between the 2 groups. Intra-operatively,their mean arterial pressure (MAP),heart rate(HR),body temperature(T),blood oxygen saturation (SaO 2) and osmotic pressure of plasma (Osm) were observed dynamically based on the operating time. Results In patients of unwarmed group,after 45 min in the process of TURP,on an average MAP was decreased by 7.6 mm Hg (1 mm Hg=0.133 kPa;F=1.334,P=0.262);HR was reduced by 21.6/min (P
9.Advance in research on anti-tumor of endostatin
Zhiming ZHANG ; Jingping YU ; Suping SUN
Journal of International Oncology 2011;38(5):345-348
Endostatin is an antitumor molecular targeting angiogenesis of tumor and plays an inhibitory role in tumorigenesis through inhibiting pathological angiogenesis, proliferation and metastasis of tumor. As increasing drugs for targeting therapy aim in clinic, endostatin has become one of hot spots of research on combined treatment of cancer in recent years.
10.Characteristics of obesity and role of ERK1/2 and p38MAPK in produetion of adipose tissue in rat with metabolic syndrome
Yaping ZHANG ; Zhiming ZHU ; Zhencheng YAN
Medical Journal of Chinese People's Liberation Army 2001;0(08):-
Objective To observe the characteristics of obesity in rats with metabolic syndrome (MS) and to study the role of ERK1/2 and p38MAPK in the production of adipose tissues. Methods The metabolic parameters, weight of adipose tissue, waist circumference (WC), Lee's index, distribution area of adipocytes and the number of big adipocytes in the rats were measured. Adipose tissues and liver were histopathologically observed. ERK1/2 and p38MAPK of adipose tissue were determined using Western blot. Results (1) The blood pressure, fasting insulin level, LDL-C, TG and FFA in rats with MS were markedly higher than that in control rats (P