Objective To study and find the reason and treatment way of severe encephalocele after operation on the brain. Me thods 15 cases who have severe encephalocele were reviewed retrospec tively.Results Among 15 cases, 12 cases have been cured,3 c ases died with severe pulmonary infection.Conclusion The re asons of severe encephalocele are hydrocephalus?cerebral edema and intracranial infection and it is useful to utilize medicine and other ways to lighten hydroc ephalus?cerebral edema and intracranial infection for the patients who have sev ere encephalocele.

Ohiective To establish an in vitro test system for testing CMV specific immune response. Methods Human embryonic lung fibroblasts(HELF)infected with CMV-AD169 and a mutant strain RV-TB40E-4, respectively, were stained with anti-HLA-A * 0201-PE and then measured for the expression of HLA-A * 0201. The infected HELF were incubated with individual pp65 peptide NLVPMVATV and cultured together with PBMC,then intracellular IFN-γ was tested by flow cytometry(FCM). Results The expression of HLA-A * 0201 was markedly suppressed on AD169-infected fibroblasts, while it was not affected on RV-TB40E-4-infected cells. PBMC showing no response to AD169-infected cells responded significantly to RV-TB40E-4-infected cells. The HELF infected with AD169 and loaded with external peptides induced a significantly stronger response which was the sum of the induced response ratios of the infected HELF and the uninfected HELF loaded with peptides. Conclusion CMV-AD169 downregulates the expression of MHC Ⅰ, while it does not decrease the capacity of cells to present external peptides. RV-TB40E-4,a CMV mutant in which the gene of US2-6/US11 has been deleted, does not affect MHC Ⅰ expression, and therefore represents a more suitable tool to explore CMV-specific immune response.

This article summarizes transforming growth factor-β1 in the primary hepatocellular carcinoma's occurrence and development, and carries on the summary to its possible mechanisms, and explains the research progress and the hot spot at present, then discusses the questions.

Objective To analyze gene expression profile for exploring the function and regulatory network of differ-entially expressed genes in prostate cancer by bioinformatics. Methods The data of gene expression profile in prostate cancer were obtained from GEO database. R software and affy, limma, pheatmap, ggplot2 and other R packages were applied for data mining and bioinformatics analysis. Combined with DAVID and GeneMANIA , dif-ferentially expressed genes and their regulatory networks were annotated. Results These differentially expressed genes with statistical significance were 56 genes, 15 upregulated genes, 41 downregulated genes;these genes were enriched into different subgroups. cav1, slc16a2, cav2, slc16a5, magi2, ptrf, pdlim5, lmod1 and abcc6 were en-riched into the cell membrane component subgroup ofcell component category. cav1, cav2 and ptrf regulated the function of caveolae, they may play an important role in the occurrence and development of prostate cancer. Conclusion Differentially expressed genes between prostate cancer and adjacent tissues assemble a complex regu-latory network. Bioinformatics is a tool for data mining of the regulatory network , which provides ideas and data for the molecular mechanisms in prostate cancer.

Objective To study the influence of aldehydes and tannic acid on soft capsules, approach how to prevent soft capsules from delayed disintegration. Methods Add aldehydes and tannic acid to soft capsules material ingredient, to proceed accelerating stability trial and test the disintegration time. Result Little increase of aldehydes amount had great effect on the disintegration time of soft capsules, different aldehydes and tannic acid had different effect on the disintegration time, adding some organic acid such as fumaric acid to the material would improve disintegrating property. Conclusion The amount of aldehydes and tannic acid contained in the material and ingredient should be controlled strictly when prepare soft capsules so that the disintegration time of capsules could be complying with the Pharmacopeia.

Objective To evaluate the laparoscopy in the diagnosis and treatment for acute abdominal emergency. Methods A retrospective analysis was conducted on clinical data of 35 patients with an acute abdomen of unknown causes diagnosed and treated under laparoscope from December 1999 to April 2003 in this hospital. Results Diagnosis was confirmed by laparoscopy in all the 35 cases. Laparoscopic operation was successfully completed in 28 cases, whereas a conversion to open surgery was required in 7 cases, in which the site of incision was under the guidance of laparoscopic observations. No surgery-related deaths or wound-related complications occurred. The mean operative time was 48 min (range, 30~72 min), and the mean hospital stay was 5.8 days (range, 3~9 days). Conclusions Laparoscopy is not only a safe and accurate diagnostic method, but also a good procedure for treatment.

Objective To investigate the relationship between galectin-3 and tumour metastasis, and the future prospect of galectin-3 in clinic.Methods Related articles were reviewed. Results Galectin-3, a member of the ?-galactoside-binding proteins, is expressed widely in epithelial and immune cells, and interacts with intracellular glycoproteins, cell surface molecules and extracellular matrix proteins. Galectin-3 is involved in various biological phenomena including cell growth, adhesion, differentiation, angiogenesis and apoptosis, and is associated with invasion and metastasis of tumour. Conclusion Because of the correlation between galectin-3 and tumour invasion and metastasis, galectin-3 may act as the diagnostic marker for tumour metastasis and one of the target proteins for cancer treatment.

Objective To investigate the molecular mechanism of peritoneal dissemination of gastric cancer. Methods Literatures in recent years about mechanisms of peritoneal metastasis in gastric cancer were reviewed and summarized.Results Peritoneal metastasis related to viability of cancer cells and peritoneal characteristics. Moreover, it is necessary that many adhesive moleculars, protein hydrolase, cell factors and vascular factors involved in peritoneal metastasis.Conclusion Peritoneal metastasis of gastric cancer was induced by multiple factors together.

Objective To investigate the correlation between resistin,adiponectin and the blood stasis syndrome in diabetic and primary hypertension patients.Method The primary hypertension and diabetes patients were drawn vein blood to make plasma and serum.Enzyme linked immunosorbent assay(ELISA) method was used in checking serum adiponectin and resistin.Radioimmuoassay method was applied in checking serum insulin,leptin,ET and tumor necrosis factor(TNF).The fasting blood sugar,total cholesterol(TC),triamid-glycerin(TG),high dense lipoprotein-cholesterol(HDL) and low dense lipoprotein-cholesterol(LDL) were detected by the chemi-enzymic method.The relation between the indexes and blood stasis syndrome was analyzed by SAS9.1 for windows.Results The detectable rate of the blood stasis syndrome in the diabetic patients was 0.423,while 0.246 in the primary hypertension patients.The difference was significant in statistics.Incidence of blood stasis syndrome increased with the course of illness.Compared with the non-blood stasis syndrome group,the content of leptin,ET and TNF in the blood stasis syndrome group significantly raised,but the content of adiponectin and insulin diminished.The content of resistin did not have obviously change.Compared with the primary hypertension patients,the level of leptin,adiponectin,resistin,insulin in the diabetic patients raised,but there wasn't significant difference in the level of ET and TNF.Conclusions There is negative correlation between adiponectin and the blood stasis syndrome.The link between obesity and the blood stasis syndrome may be through fat factor regulating vasoactive factor.The blood stasis syndrome caused by obesity maybe have nothing to do with the consistency of resistin.

Objective To compare the clinical efficiency between laparoscopic enterodialysis and open enterodialysis. Methods Clinical data of 25 cases of adhesive intestinal obstruction treated by laparoscopic enterodialysis (Laparoscopic Group) from December 1999 to December 2002 were retrospectively reviewed and compared with clinical records of 23 cases receiving open enterodialysis (Open Group) in the same period. The operating time, intra-operative blood loss, incidence of complications, postoperative recovery time of bowel movement and length of hospital stay of the two groups were compared, respectively. Results In the Laparoscopic Group operation was successfully accomplished in 23 cases while a conversion to open surgery was required in 2 cases. Of the Laparoscopic Group and the Open Group, the operation time was (58.3?8.1) min and (84.0?7.5) min (t=11.383, P=0.000), respectively; the intra-operative blood loss was (31.4?5.1) ml and (192.6?26.4) ml (t=29.995, P=0.000), respectively; the postoperative hospital stay was (4.1?1.4) days and (9.7?2.0) days (t=11.413, P=0.000), respectively; the postoperative recovery time of bowel function was (19.6?2.2) hours and (49.0?8.8) hours (t=16.207, P=0.000), respectively and the postoperative complications were seen in 1 case and 9 cases (?2=6.960, P=0.008), respectively. Conclusions Compared with open enterodialysis, laparoscopic enterodialysis has advantages of short operation time, less blood loss, rapid recovery and fewer complications.