BACKGROUND: The root canal filling materials available have different degrees of noxious stimulation to periapical tissue and cannot induce growth and regeneration of bone at the periapical region. Moreover, the operation of canal filling is not easily controlled.OBJECTIVE: To investigate biocompatibility and osteoconductivity between periapical tissue and compound coral paste after root canal filling.DESIGN: Controlled observation.SETTING: Department of Stomatology in the Renmin Hospital of Wuhan University.MATERIALS: The experiment was carried out in the Key Laboratory for Oral Biomedical Engineering by the State Ministry of Education, School of Stomatology in Wuhan University from September 2002 to January 2006. Eight adult female mongrel dogs, weighed 15 kg, were offered by the Wuhan General Hospital of Guangzhou Military Area Command of Chinese PLA (SYXK-2003-0007). Each dog contained 32 root canals, and totally 256 root canals were utilized in this study.METHODS: Experimental animals underwent general anesthesia by the intravenous injection of sodium pentobarbital (30 mg/kg), then tracheal intubation was utilized for the root canal filling, and the canals were randomly distributed into two groups: experimental group (n=128) with compound coral paste (coral 40 g and iodoform 8 g, offered by the Key Laboratory for Oral Biomedical Engineering by the State Ministry of Education, School of Stomatology in Wuhan University); control group (n=128) with gutta-percha point+zinc oxide-eugenol sealer. One animal was sacrificed by anesthetic overdose at 2, 4, 12 and 24 weeks after root canal filling, dental film by X-ray and light microscope were used for the examination.MAIN OUTCOME MEASURES: ①Periapical inflammation: Cells were counted in a high power field, and three levels were defined as mild (＜100 cells/mm2), moderate (100-200 cells/mm2) and severe (＞200 cells/mm2).②destruction and regeneration of periapical tissues.③bone substitute of compound coral paste in periapical regions.RESULTS: Eight mongrel dogs were all involved in the result analysis.①Periapical shadow by X-ray film: Distinct shadow at root apex area can not be observed in the experimental group at each stage. While two cases appeared the root apex shadow in the control group at 12 and 24 weeks, respectively.②Histopathological observation: At 2 weeks after root filling,inflammatory cell infiltration was observed in each group, which was dominantly neutrocyte. There was a mild inflammation in experimental group and a moderate inflammation in control group. Four weeks after root filling, there were focal inflammatory cells infiltrated around the coral particles in the experimental group, but in the control group there were a great deal of inflammatory cells in periapical tissue. Twelve weeks after root filling, in experimental group, there was no inflammatory cell infiltration was observed, the deposition of bone combined tightly with coral particles was detected, and apical foramen became smaller;, but in the control group, there were still inflammatory cells circumvohiting the gutta-percha point. After 24 weeks, coral particles was not observed at the root filling region in the experimental group, and they were replaced by a great deal deposition of bones, root foramens were sealed completely and grew into root canal wall. Root apex was coated with fibrous tissue in the control group.CONCLUSION: This compound coral paste shows good compatibility after filling, promotes the osteoconductivity, and seals the root foremen, so it can be used as a root canal filling material.

ObjectiveTo testify the therapeutic efficacy of acupuncture in treating diarrhea-predominant irritable bowel syndrome (D-IBS) based on brain-gutinteraction.MethodEighty D-IBS patients were randomized into brain-gut interacted acupuncture group (treatment group) and a group with acupoints selected based on conventional syndrome differentiation (control group), 40 in each group. The treatment group was intervened by acupuncture by selecting the thirteen ghost acupoints (invented by SUN Si-miao) plus Tianshu (ST25), Dachangshu (BL25), etc., while the control group was by acupuncture based on conventional point selection, including Zusanli (ST36),Tianshu (ST25), Dachangshu (BL25), Ganshu (BL18), Pishu (BL20), etc., three times a week, and 10 sessions as a treatment course. The clinical efficacies were evaluated after 2 treatment courses.ResultThe two groups were generally equal to each other incomparing each dimension of the Short-form Health Survey (SF-36) before treatment, while the scores were increased after treatment in both groups; the treatment group was significantly superior to the controlgroup in comparing the scores ofrole-physical,bodily pain, general health, vitality, social role functioning, and reported health transition from the SF-36 (P<0.05).ConclusionAcupuncture based on brain-gut interaction can produce a more significant effect in treating D-IBS compared to conventional acupuncture.

BACKGROUND:Spinal internal fixation is widely used in the treatment of lumbar spondylolisthesis, with the purpose of lumbar fusion, reconstruction of lumbar sagittal sequences, relieving spinal cord or nerve root compression. OBJECTIVE:To review the biocompatibility of lumbar spinal implants applied in the treatment of degenerative lumbar spondylolisthesis. METHODS:A computer-based retrieval of PubMed and CNKI was performed for relevant articles published from January 2000 to January 2015. The keywords were biocompatibility, degenerative lumbar spondylolisthesis, implant in English and Chinese, respectively, which appeared in the title and abstract. RESULTS AND CONCLUSION:Lumbar spinal implants commonly used include pedicle screws and interbody fusion cages. The fusion cages are mainly classified as biological fusion cage, metal fusion cage, composite fusion cage and absorbable fusion cage. Internal fixation implants made of different materials have different biocompatibilities, but also develop different stabilities. With the development of bionics, 3D printing technology, biomechanics and materials science, lumbar spinal implants wil have better biocompatibility, bone substitute capability and biomechanical properties.

Recent studies have demonstrated that GFI1B is most prominently expressed in cells of the fetal and adult erythroid and megakaryocytic lineages. In 2006,it is first reported that GFI1B is highly expressed in acute myeloid leukemia, especially in erythroleukaemia and megakaryocytic leukaemia.This report provides theoretical basis for diagnosis and differential diagnosis of subtypes of acute myeloid leukaemia.In this review,we covered the expression of GFI1B in leukemia,T-cell lymphoma,myelodysplastic syndrome and so on.

Objective To investigate the therapeutic effect of Ganglioside(GM1) on acute cerebral infarction.Methods 60 cases with acute cerebral infarction were divided into two groups randomly.At the basic of routin treatment for the twe gyoups,GM1 group was treated with GM1 40 mg/d for 14 days and control group was treated with Citicoline 0.75 g/d for 14 days.The neurological deficit scores(NDS),activities of daily living(ADL) and adverse reactions were evaluated.Results After treatment,the scores of NDS and ADL were singnificantly decreased in twe groups than before treatment((P

Objective To analyze and compare the values of applying color Doppler ultrasound in the diag -nosis of testicular torsio and acute epididymo -orchitis.Methods 90 males in our hospital were selected and divided into the testicular torsion group and acute epididymo -orchitis group ,color Doppler ultrasound was applied in the two groups.Results After examination by color Doppler ultrasound ,it showed that there were significant differences in blood supply of testicle ,epididymis between the two groups ,the diagnostic result was accurate with statistical signifi-cance(χ2 =11.408,11.944,P<0.05).Conclusion It can highlight the images and blood supply features of testic-ular torsion and acute epididymo -orchitis that applying color Doppler ultrasound ,and as an economic ,practical ,accu-rate and non-invasive examination ,it can diagnose testicular torsion and acute epididymo -orchitis accurately ,so it's the preference for testicular torsio and acute epididymo -orchitis.

0.05 ).The occlusal caries (73.08%) was more than the interproximal(50.00%).Conclusion:The prevalence of dental caries in the people at the Neolithic Age was lower than that in modern people.

Objective:To isolate and purify glucocorticoid receptor(GR) of rat liver with HPHIC and verify the existence of the low affinity GR as the further evidence for theoretical basis of large dose glucocorticoid treatment.Methods:The supernatant of the rat liver was performed with Pseudoscatchard assay and HPHIC analysis after ultracentrifuge.SDS PAGE and Western blot were used to identify GR.Results:At the standard atmosphere,when the temperature was at 0 4℃,only one peak appeared at 9 11 min.When the temperature was at 25℃ and the concentration of 3H Dex exceeded 50 nmol/L,two peaks appeared at 3 7 min and 9 11 min.The protein was verified with SDS PAGE and Western blot, its molecular weight was 60 000,which could be bound with monoclonal antibody of GR.Conclusion:Two peaks of GR appears with HPHIC analysis, suggesting they may be high and low affinity receptor.Further researches are necessary to ascertain it. [

Objective To observe the clinical effect of resin adsorbing devices combined with hemodialysis in the treatment for renal osteopathy in chronic renal failure patients undergoing maintenance hemodialysis.Methods Fifty two patients undergoing maintenance hemodialysis for at least 2 years,admitted from January 2004 to October 2006,were divided into three groups:group A,resin adsorbing devices combined with hemodialysis group(HP+HD);group B,hemodiafiltration group(HDF+HD);group C,active vitamin D peroral group(Oral vitamin D+HD).The changes in serum parathyroid hormone(iPTH)and phosphorus,urea nitrogen(BUN),creatinine(SCr),hemoglobin(Hb)were compared among the three groups before and after the treatment.Results No significant difference was found in BUN,SCr,and Hb contents among the three groups before and after the treatment.Compared with the data before the treatment,iPTH and phosphorus were decreased significantly after 12 weeks of treatment in group A(P

To observe the effects of melatonin on postacyclin concentration, nitric oxidase activity(NOS) and inducible nitric oxidase(iNOS) mRNA expression in calf aortic endothelial cells in medium with high concentration of glucose, endothelial cells of calf thoracic arota were cultured and divided into five groups. Group A was a low-glucose control with 5.5mmol/L glucose in medium. Group B was a high-glucose control with 33mmol/L glucose in medium. Cells of group C was cultured with 33mmol/L glucose and 10 -13mol/L melatonin, those of group D was cultured with 33mmol/L glucose and 10 -9mol/L melatonin, and those of group E was cultured with 33mmol/L glucose and 10 -5mol/L melatonin. The concentration of 6-keto-PGF 1?, metabolizing product of PGI 2, in the culture medium was measured with radio-immunological assay. NOS activity was determined with colorimetry and iNOS mRNA was measured with semi-quantitative RT-PCR. 6-keto-PGF 1? concentration, NOS activity and iNOS mRNA expression in group A were significantly lower than those in group B(P0.05). 6-keto-PGF 1? concentration of group D and group E was significantly higher than that in group B(P