Objective To analyze the efficiency of current outpatient billing service quantitatively.Methods The author applied mathematical model and arithmetic of the queuing theory to estimate the selective sample of gynecologic outpatient charge.The average arrival rate of patient and average charge were counted.By using the queuing theory and relevant assisted program,we got the corresponding evaluation index.Results It is reasonable for the charge of current gynecologic outpatient.Conclusion It is feasible to apply queuing theory to quantitative analysis on outpatient charge service in the hospital.

There are many options on the No.1 Military Medical Project database backup in the Hospital Information System.The DataGuard of Oracle8i is one of them.This article introduces the concepts and principles of DataGuard,and briefly describes the implementation procedure and some attention-deserved problems.DataGuard is compared with traditional method for data back-up,especially the important improvement based on Oracle7 and Standby.

Genome ; Humans ; Precision Medicine

Objective To investigate the inhibitory effect of SFAS on the growth of human hepatic carcinoma cell line SMMC-7721 in vitro and discuss its mechanism. Methods The inhibitory fraction of SMMC-7721 cells was measured by MTT assay, and both of the Annexin V and TUNEL assays were used to determine the apoptotic proportion. Results The value of the control group was (0.385?0.03), and that of the SFAS groups (250 ?g/ml, 500 ?g/ml, 1 mg/ml and 2 mg/ml) were (0.31?0.019), (0.199?0.022), (0.150?0.033) and (0.048?0.026) respectively, with significant difference statistically ( P

OBJECTIVE:To investigate osteogenic and chondrogenic differentiation of adipose-derived stem cells following a short time induction with bone morphogenetic protein 2(BMP-2) or BMP-7.METHODS:The subcutaneous adipose tissue was obtained from adult New Zealand rabbits under aseptic condition,and cultured in vitro with collagenase digestion.All cells were divided into 3 groups:in the BMP-2 group,cells were cultured with medium containing 0.1 g/L vitamin C,10 mmol/L?-sodium glycerophosphate and 10?g/L BMP-2 for 10 minutes,followed by 4-14 days inoculation with density of 18?104 cells per pore.In the BMP-7 group,cells were cultured with BMP-7 with the same methods as BMP-2 group.The cells were cultured with simple culture medium in the control group.RESULTS:Compared to the control group,the number of adipose-derived stem cells,protein level and ALalkaline phosphatase(ALP) activity of BMP-2 and BMP-7 groups was up-regulated 1.78-1.79,1.15-1.95,and 32-40 times,respectively.At days 4 after a 15 minutes induction,the runx-2 gene expression,osteopontin gene,and biglycan gene were increased 1.9,2.2 and 1.3-1.7 folds than that of the control group.Meantime,only the biglycan gene expression was increased 1.3-1.7 folds in the BMP-7 group,the runx-2 gene expression,osteopontin gene was not changed.At day 14 after a 15 minutes induction,there was no alter of runx-2,osteopontin,biglycan,as well as aggrecan gene expression in the BMP-2 group;while down-regulated runx-2,osteopontin,biglycan 1.8,5.0 and 1.7 folds,with increased aggrecan gene in the BMP-7 group.CONCLUSION:Following a short time induction,BMP-2 can stimulate runx-2 and osteogenic expression at 4 days after re-culture,whereas BMP-7 down-regulate genes expression at days 14,yet down-regulate aggrecan mRNA expression.

Aging is a biological process and state characterized by morphological change, hypofunction and metabolic disorder, often accompanied by severe degenerative diseases, including amyotrophy, atherosclerosis, neurodegeneration, and so on. Age-related diseases not only decrease the life quality of the elderly, but also impose great burden on family and society. As population aging, we are increasingly coming to realize that the longevity we want should be healthy aging but not simple increase of lifespan. By researching on the yeast, C. elegans, drosophila, mice and humans, we get to know the mechanisms of aging and methods for anti-aging, such as dietary restriction and use of anti-aging drugs like rapamycin. In this review, we summarized mainly the studies on mechanisms of aging and elaborate associated anti-aging strategies and underlying mechanisms.

Objective To observe the clinical effect of resin adsorbing devices combined with hemodialysis in the treatment for renal osteopathy in chronic renal failure patients undergoing maintenance hemodialysis.Methods Fifty two patients undergoing maintenance hemodialysis for at least 2 years,admitted from January 2004 to October 2006,were divided into three groups:group A,resin adsorbing devices combined with hemodialysis group(HP+HD);group B,hemodiafiltration group(HDF+HD);group C,active vitamin D peroral group(Oral vitamin D+HD).The changes in serum parathyroid hormone(iPTH)and phosphorus,urea nitrogen(BUN),creatinine(SCr),hemoglobin(Hb)were compared among the three groups before and after the treatment.Results No significant difference was found in BUN,SCr,and Hb contents among the three groups before and after the treatment.Compared with the data before the treatment,iPTH and phosphorus were decreased significantly after 12 weeks of treatment in group A(P

Objective To establish a stable transplanted model of Vx 2 carcinoma in rabbit from three different transplanted methods, and analyze the DSA (digital substraction angiography) features of the implanted tumors. Methods 60 white rabbits of New Zealand were divided into 3 groups at random, 20 for each group. In the first group, Vx 2 carcinoma cells (about 5?10 7 )were infused via hepatic artery into the rabbit liver. For the second group, Vx 2 carcinoma cells (about 5?10 7 ) were inplanted into the left lobe of the liver via laparotomic route and in the 3rd group with the same route as the 2nd group, a piece of tumor tissue (contained about 10 6 10 8 carcinoma cells) were implanted into the left lobe of the liver. And then a comparative observation was performed in 3 groups included:1. the successful rate of implanted tumors; 2. the volume change and the growth rate of implanted tumors; 3. the biological features of implanted tumors in histopathology and electronic microscope photographs and DSA features of implanted tumors. Results The successful rates of transplanted tumors in the three groups were 7/20, 10/20 and 19/20 respectively. The successful rate of the 3rd group was the highest of the 3( P

Objective To study MRI manifestation of lymphoma of bone marrow. Methods Ten patients(8 males and 2 females) with lymphoma of bone were performed MRI. Two cases were primary (1 HD and 1 NHL) and 8 were secondary NHL. Results (1)Location: All cases involved axial bones; (2)Number: 9 cases involved multiple bones, only 1 primary NHL involved solitary bone; (3)Signal Intensity: The marrow infiltration were showed low and high signal intensity on T 1WI and T 2WI respectively; (4)Shape: 9 cases were showed multiple focal features, another one showed diffuse changes; (5)Pathological Fracture: 3 cases associated with pathological fractures of vertebrae. Conclusion The sensitivity of MRI was excellent for detecting marrow infiltration of lymphoma, but the specificity was poor.

BACKGROUND: As indicated by clinical research, if cerebral infarction could be effectively treated at early stage, especially normal specific therapy provided within 6 hours or even earlier after attack, the prognosis would be significantly better than delayed therapy. However, it is still unclear that whether the changes of cellular apoptosis-inducing or -inhibiting indicators could be used as criteria in the judgment of prognosis.OBJECTIVE: To test the content of cell apoptotic factor and to investigate the prognosis in hospitalized patients with cerebral infarction who received treatment at different time for further verification of the therapeutic timing for the disease.DESIGN: A same term randomized controlled study based on patients.SETTING: Department of neurology of a general hospital of a military area command of Chinese PLA.PARTICIPANTS: Totally 144 male patients admitted in the Second Department of Neurology, General Hospital of Jinan Military Area Command of Chinese PLA between 2000 and 2002 were divided into four groups including 6 hours, 24 hours, 72 hours and 96 hours group according to different time of therapy provided.METHODS: Oral administration of 400 mg Lumbrokinase, 2 tablets of heparin sodium, 60 mg of nimodipine, and 100 mg of vitamin E, three times a day. 150 mL of normal saline(NS) containing 52.5 mg of Ginkgo biloba L extractive(Jin Na Duo) and 150 mL of NS containing 10 mL of Cerebroprotein Hydeolysate were used through intravenous drop once a day. Ten days were set as one therapeutic course and 2 courses were given. 200 g/L of mannite was given to dehydrate for patients with large area infarction(＞ 7 cm2) . Platelet membrane Fas, Apo2.7 and Bcl-2 percentage and prognostic assessment were tested in patients of four groups before and after therapy.MAIN OUTCOME MEASURES: Peripheral platelet membrane Fas,Apo2.7 and Bcl-2 percentage in patients of different group and prognosis evaluation.RESULTS: Percentage of platelet membrane Fas, Apo2.7 and Bcl-2 of 6 hours group was significantly higher or lower after therapy than before therapy ( P ＜ 0.05 ), and moreover, the difference with other groups was significant( P ＜ 0.05), As revealed in the analysis of prognosis, the effectiveness of patients who received therapy within 6 hours was significantly better than that of 96 hours group and the mortality reduced significantly.CONCLUSION: Normal hospitalizing therapy provided within 6 hours after attack could surely improve the prognosis and reduce the disability rate, and the abnormity and extent in Fas, Apo2.7 and Bcl-2 are closely correlated with prognosis.